Abstract

Classically, optical systems are considered to have a fundamental resolution limit due to wave nature of light. This article presents a novel method for observing sub-wavelength features in a conventional optical microscope using linear optics. The operation principle is based on a random and time varying flow of nanoparticles moving in proximity to the inspected sample. Those particles excite the evanescent waves and couple them into harmonic waves. The sub-wavelength features are encoded and later on digitally decoded by proper image processing of a sequence of images. The achievable final resolution limit corresponds to the size of the nanoparticles. Experimental proof of principle validation of the technique is reported.

© 2010 OSA

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2009 (2)

G. Maire, F. Drsek, J. Girard, H. Giovannini, A. Talneau, D. Konan, K. Belkebir, P. C. Chaumet, and A. Sentenac, “Experimental demonstration of quantitative imaging beyond Abbe’s limit with optical diffraction tomography,” Phys. Rev. Lett. 102(21), 213905 (2009).
[CrossRef] [PubMed]

Z. Zalevsky, E. Fish, N. Shachar, Y. Vexberg, V. Mico, and J. Garcia, “Super-resolved imaging with randomly distributed, time- and size-varied particles,” J. Opt. A, Pure Appl. Opt. 11(8), 085406 (2009).
[CrossRef]

2008 (4)

2007 (3)

K. Fujita, M. Kobayashi, S. Kawano, M. Yamanaka, and S. Kawata, “High-resolution confocal microscopy by saturated excitation of fluorescence,” Phys. Rev. Lett. 99(22), 228105 (2007).
[CrossRef]

S. Bretschneider, C. Eggeling, and S. W. Hell, “Breaking the diffraction barrier in fluorescence microscopy by optical shelving,” Phys. Rev. Lett. 98(21), 218103 (2007).
[CrossRef] [PubMed]

Y. Kuznetsova, A. Neumann, and S. R. J. Brueck, “Imaging interferometric microscopy-approaching the linear systems limits of optical resolution,” Opt. Express 15(11), 6651–6663 (2007).
[CrossRef] [PubMed]

2006 (5)

S. A. Alexandrov, T. R. Hillman, T. Gutzler, and D. D. Sampson, “Synthetic aperture fourier holographic optical microscopy,” Phys. Rev. Lett. 97(16), 168102 (2006).
[CrossRef] [PubMed]

M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods 3(10), 793–796 (2006).
[CrossRef] [PubMed]

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

S. T. Hess, T. P. K. Girirajan, and M. D. Mason, “Ultra-high resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J. 91(11), 4258–4272 (2006).
[CrossRef] [PubMed]

O. Gliko, G. D. Reddy, B. Anvari, W. E. Brownell, and P. Saggau, “Standing wave total internal reflection fluorescence microscopy to measure the size of nanostructures in living cells,” J. Biomed. Opt. 11(6), 064013 (2006).
[CrossRef]

2005 (5)

V. Westphal and S. W. Hell, “Nanoscale resolution in the focal plane of an optical microscope,” Phys. Rev. Lett. 94(14), 143903 (2005).
[CrossRef] [PubMed]

M. G. L. Gustafsson, “Nonlinear structured-illumination microscopy: wide-field fluorescence imaging with theoretically unlimited resolution,” Proc. Natl. Acad. Sci. U.S.A. 102(37), 13081–13086 (2005).
[CrossRef] [PubMed]

E. Baleine and A. Dogariu, “Variable coherence scattering microscopy,” Phys. Rev. Lett. 95(19), 193904 (2005).
[CrossRef] [PubMed]

T. Kalkbrenner, U. Håkanson, A. Schädle, S. Burger, C. Henkel, and V. Sandoghdar, “Optical microscopy via spectral modifications of a nanoantenna,” Phys. Rev. Lett. 95(20), 200801 (2005).
[CrossRef] [PubMed]

Y. Garini, B. J. Vermolen, and I. T. Young, “From micro to nano: recent advances in high-resolution microscopy,” Curr. Opin. Biotechnol. 16(1), 3–12 (2005).
[CrossRef] [PubMed]

2003 (1)

W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21(11), 1369–1377 (2003).
[CrossRef] [PubMed]

2001 (1)

D. Toomre and D. J. Manstein, “Lighting up the cell surface with evanescent wave microscopy,” Trends Cell Biol. 11(7), 298–303 (2001).
[CrossRef] [PubMed]

2000 (3)

M. G. L. Gustafsson, “Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy,” J. Microsc. 198(2), 82–87 (2000).
[CrossRef] [PubMed]

J. T. Frohn, H. F. Knapp, and A. Stemmer, “True optical resolution beyond the Rayleigh limit achieved by standing wave illumination,” Proc. Natl. Acad. Sci. U.S.A. 97(13), 7232–7236 (2000).
[CrossRef] [PubMed]

T. D. Lacoste, X. Michalet, F. Pinaud, D. S. Chemla, A. P. Alivisatos, and S. Weiss, “Ultrahigh-resolution multicolor colocalization of single fluorescent probes,” Proc. Natl. Acad. Sci. U.S.A. 97(17), 9461–9466 (2000).
[CrossRef] [PubMed]

1999 (1)

M. G. L. Gustafsson, D. A. Agard, and J. W. Sedat, “I5M: 3D widefield light microscopy with better than 100 nm axial resolution,” J. Microsc. 195(1), 10–16 (1999).
[CrossRef] [PubMed]

1994 (1)

1992 (1)

S. Hell and E. H. K. Stelzer, “Fundamental improvement of resolution with a 4Pi-confocal fluorescence microscope using two-photon excitation,” Opt. Commun. 93(5-6), 277–282 (1992).
[CrossRef]

1991 (1)

E. Betzig, J. K. Trautman, T. D. Harris, J. S. Weiner, and R. L. Kostelak, “Breaking the diffraction barrier: optical microscopy on a nanometric scale,” Science 251(5000), 1468–1470 (1991).
[CrossRef] [PubMed]

1986 (1)

1966 (1)

1873 (1)

E. Abbe, “Beiträge zur Theorie des Mikroskops und der mikroskopischen Wahrnehmung,” Archiv für Mikroskopische Anatomie 9(1), 413–418 (1873).
[CrossRef]

Abbe, E.

E. Abbe, “Beiträge zur Theorie des Mikroskops und der mikroskopischen Wahrnehmung,” Archiv für Mikroskopische Anatomie 9(1), 413–418 (1873).
[CrossRef]

Agard, D. A.

L. Shao, B. Isaac, S. Uzawa, D. A. Agard, J. W. Sedat, and M. G. L. Gustafsson, “I5S: wide-field light microscopy with 100-nm-scale resolution in three dimensions,” Biophys. J. 94(12), 4971–4983 (2008).
[CrossRef] [PubMed]

M. G. L. Gustafsson, D. A. Agard, and J. W. Sedat, “I5M: 3D widefield light microscopy with better than 100 nm axial resolution,” J. Microsc. 195(1), 10–16 (1999).
[CrossRef] [PubMed]

Alexandrov, S. A.

S. A. Alexandrov, T. R. Hillman, T. Gutzler, and D. D. Sampson, “Synthetic aperture fourier holographic optical microscopy,” Phys. Rev. Lett. 97(16), 168102 (2006).
[CrossRef] [PubMed]

Alivisatos, A. P.

T. D. Lacoste, X. Michalet, F. Pinaud, D. S. Chemla, A. P. Alivisatos, and S. Weiss, “Ultrahigh-resolution multicolor colocalization of single fluorescent probes,” Proc. Natl. Acad. Sci. U.S.A. 97(17), 9461–9466 (2000).
[CrossRef] [PubMed]

Anvari, B.

O. Gliko, G. D. Reddy, B. Anvari, W. E. Brownell, and P. Saggau, “Standing wave total internal reflection fluorescence microscopy to measure the size of nanostructures in living cells,” J. Biomed. Opt. 11(6), 064013 (2006).
[CrossRef]

Baleine, E.

E. Baleine and A. Dogariu, “Variable coherence scattering microscopy,” Phys. Rev. Lett. 95(19), 193904 (2005).
[CrossRef] [PubMed]

Bates, M.

M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods 3(10), 793–796 (2006).
[CrossRef] [PubMed]

Beck, M.

Belkebir, K.

G. Maire, F. Drsek, J. Girard, H. Giovannini, A. Talneau, D. Konan, K. Belkebir, P. C. Chaumet, and A. Sentenac, “Experimental demonstration of quantitative imaging beyond Abbe’s limit with optical diffraction tomography,” Phys. Rev. Lett. 102(21), 213905 (2009).
[CrossRef] [PubMed]

Betzig, E.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

E. Betzig, J. K. Trautman, T. D. Harris, J. S. Weiner, and R. L. Kostelak, “Breaking the diffraction barrier: optical microscopy on a nanometric scale,” Science 251(5000), 1468–1470 (1991).
[CrossRef] [PubMed]

Bonifacino, J. S.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

Bretschneider, S.

S. Bretschneider, C. Eggeling, and S. W. Hell, “Breaking the diffraction barrier in fluorescence microscopy by optical shelving,” Phys. Rev. Lett. 98(21), 218103 (2007).
[CrossRef] [PubMed]

Brownell, W. E.

O. Gliko, G. D. Reddy, B. Anvari, W. E. Brownell, and P. Saggau, “Standing wave total internal reflection fluorescence microscopy to measure the size of nanostructures in living cells,” J. Biomed. Opt. 11(6), 064013 (2006).
[CrossRef]

Brueck, S. R. J.

Burger, S.

T. Kalkbrenner, U. Håkanson, A. Schädle, S. Burger, C. Henkel, and V. Sandoghdar, “Optical microscopy via spectral modifications of a nanoantenna,” Phys. Rev. Lett. 95(20), 200801 (2005).
[CrossRef] [PubMed]

Chaumet, P. C.

G. Maire, F. Drsek, J. Girard, H. Giovannini, A. Talneau, D. Konan, K. Belkebir, P. C. Chaumet, and A. Sentenac, “Experimental demonstration of quantitative imaging beyond Abbe’s limit with optical diffraction tomography,” Phys. Rev. Lett. 102(21), 213905 (2009).
[CrossRef] [PubMed]

Chemla, D. S.

T. D. Lacoste, X. Michalet, F. Pinaud, D. S. Chemla, A. P. Alivisatos, and S. Weiss, “Ultrahigh-resolution multicolor colocalization of single fluorescent probes,” Proc. Natl. Acad. Sci. U.S.A. 97(17), 9461–9466 (2000).
[CrossRef] [PubMed]

Cox, I. J.

Davidson, M. W.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

Dogariu, A.

E. Baleine and A. Dogariu, “Variable coherence scattering microscopy,” Phys. Rev. Lett. 95(19), 193904 (2005).
[CrossRef] [PubMed]

Drsek, F.

G. Maire, F. Drsek, J. Girard, H. Giovannini, A. Talneau, D. Konan, K. Belkebir, P. C. Chaumet, and A. Sentenac, “Experimental demonstration of quantitative imaging beyond Abbe’s limit with optical diffraction tomography,” Phys. Rev. Lett. 102(21), 213905 (2009).
[CrossRef] [PubMed]

Eggeling, C.

S. Bretschneider, C. Eggeling, and S. W. Hell, “Breaking the diffraction barrier in fluorescence microscopy by optical shelving,” Phys. Rev. Lett. 98(21), 218103 (2007).
[CrossRef] [PubMed]

Ferreira, C.

Fiolka, R.

Fish, E.

Z. Zalevsky, E. Fish, N. Shachar, Y. Vexberg, V. Mico, and J. Garcia, “Super-resolved imaging with randomly distributed, time- and size-varied particles,” J. Opt. A, Pure Appl. Opt. 11(8), 085406 (2009).
[CrossRef]

Frohn, J. T.

J. T. Frohn, H. F. Knapp, and A. Stemmer, “True optical resolution beyond the Rayleigh limit achieved by standing wave illumination,” Proc. Natl. Acad. Sci. U.S.A. 97(13), 7232–7236 (2000).
[CrossRef] [PubMed]

Fujita, K.

K. Fujita, M. Kobayashi, S. Kawano, M. Yamanaka, and S. Kawata, “High-resolution confocal microscopy by saturated excitation of fluorescence,” Phys. Rev. Lett. 99(22), 228105 (2007).
[CrossRef]

Garcia, J.

Z. Zalevsky, E. Fish, N. Shachar, Y. Vexberg, V. Mico, and J. Garcia, “Super-resolved imaging with randomly distributed, time- and size-varied particles,” J. Opt. A, Pure Appl. Opt. 11(8), 085406 (2009).
[CrossRef]

Z. Zalevsky, E. Saat, S. Orbach, V. Mico, and J. Garcia, “Exceeding the resolving imaging power using environmental conditions,” Appl. Opt. 47(4), A1–A6 (2008).
[CrossRef] [PubMed]

García, J.

Garini, Y.

Y. Garini, B. J. Vermolen, and I. T. Young, “From micro to nano: recent advances in high-resolution microscopy,” Curr. Opin. Biotechnol. 16(1), 3–12 (2005).
[CrossRef] [PubMed]

Giovannini, H.

G. Maire, F. Drsek, J. Girard, H. Giovannini, A. Talneau, D. Konan, K. Belkebir, P. C. Chaumet, and A. Sentenac, “Experimental demonstration of quantitative imaging beyond Abbe’s limit with optical diffraction tomography,” Phys. Rev. Lett. 102(21), 213905 (2009).
[CrossRef] [PubMed]

Girard, J.

G. Maire, F. Drsek, J. Girard, H. Giovannini, A. Talneau, D. Konan, K. Belkebir, P. C. Chaumet, and A. Sentenac, “Experimental demonstration of quantitative imaging beyond Abbe’s limit with optical diffraction tomography,” Phys. Rev. Lett. 102(21), 213905 (2009).
[CrossRef] [PubMed]

Girirajan, T. P. K.

S. T. Hess, T. P. K. Girirajan, and M. D. Mason, “Ultra-high resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J. 91(11), 4258–4272 (2006).
[CrossRef] [PubMed]

Gliko, O.

O. Gliko, G. D. Reddy, B. Anvari, W. E. Brownell, and P. Saggau, “Standing wave total internal reflection fluorescence microscopy to measure the size of nanostructures in living cells,” J. Biomed. Opt. 11(6), 064013 (2006).
[CrossRef]

Gustafsson, M. G. L.

L. Shao, B. Isaac, S. Uzawa, D. A. Agard, J. W. Sedat, and M. G. L. Gustafsson, “I5S: wide-field light microscopy with 100-nm-scale resolution in three dimensions,” Biophys. J. 94(12), 4971–4983 (2008).
[CrossRef] [PubMed]

M. G. L. Gustafsson, “Nonlinear structured-illumination microscopy: wide-field fluorescence imaging with theoretically unlimited resolution,” Proc. Natl. Acad. Sci. U.S.A. 102(37), 13081–13086 (2005).
[CrossRef] [PubMed]

M. G. L. Gustafsson, “Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy,” J. Microsc. 198(2), 82–87 (2000).
[CrossRef] [PubMed]

M. G. L. Gustafsson, D. A. Agard, and J. W. Sedat, “I5M: 3D widefield light microscopy with better than 100 nm axial resolution,” J. Microsc. 195(1), 10–16 (1999).
[CrossRef] [PubMed]

Gutzler, T.

S. A. Alexandrov, T. R. Hillman, T. Gutzler, and D. D. Sampson, “Synthetic aperture fourier holographic optical microscopy,” Phys. Rev. Lett. 97(16), 168102 (2006).
[CrossRef] [PubMed]

Håkanson, U.

T. Kalkbrenner, U. Håkanson, A. Schädle, S. Burger, C. Henkel, and V. Sandoghdar, “Optical microscopy via spectral modifications of a nanoantenna,” Phys. Rev. Lett. 95(20), 200801 (2005).
[CrossRef] [PubMed]

Harris, T. D.

E. Betzig, J. K. Trautman, T. D. Harris, J. S. Weiner, and R. L. Kostelak, “Breaking the diffraction barrier: optical microscopy on a nanometric scale,” Science 251(5000), 1468–1470 (1991).
[CrossRef] [PubMed]

Hell, S.

S. Hell and E. H. K. Stelzer, “Fundamental improvement of resolution with a 4Pi-confocal fluorescence microscope using two-photon excitation,” Opt. Commun. 93(5-6), 277–282 (1992).
[CrossRef]

Hell, S. W.

S. Bretschneider, C. Eggeling, and S. W. Hell, “Breaking the diffraction barrier in fluorescence microscopy by optical shelving,” Phys. Rev. Lett. 98(21), 218103 (2007).
[CrossRef] [PubMed]

V. Westphal and S. W. Hell, “Nanoscale resolution in the focal plane of an optical microscope,” Phys. Rev. Lett. 94(14), 143903 (2005).
[CrossRef] [PubMed]

S. W. Hell and J. Wichmann, “Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy,” Opt. Lett. 19(11), 780–782 (1994).
[CrossRef] [PubMed]

Henkel, C.

T. Kalkbrenner, U. Håkanson, A. Schädle, S. Burger, C. Henkel, and V. Sandoghdar, “Optical microscopy via spectral modifications of a nanoantenna,” Phys. Rev. Lett. 95(20), 200801 (2005).
[CrossRef] [PubMed]

Hess, H. F.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

Hess, S. T.

S. T. Hess, T. P. K. Girirajan, and M. D. Mason, “Ultra-high resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J. 91(11), 4258–4272 (2006).
[CrossRef] [PubMed]

Hillman, T. R.

S. A. Alexandrov, T. R. Hillman, T. Gutzler, and D. D. Sampson, “Synthetic aperture fourier holographic optical microscopy,” Phys. Rev. Lett. 97(16), 168102 (2006).
[CrossRef] [PubMed]

Isaac, B.

L. Shao, B. Isaac, S. Uzawa, D. A. Agard, J. W. Sedat, and M. G. L. Gustafsson, “I5S: wide-field light microscopy with 100-nm-scale resolution in three dimensions,” Biophys. J. 94(12), 4971–4983 (2008).
[CrossRef] [PubMed]

Kalkbrenner, T.

T. Kalkbrenner, U. Håkanson, A. Schädle, S. Burger, C. Henkel, and V. Sandoghdar, “Optical microscopy via spectral modifications of a nanoantenna,” Phys. Rev. Lett. 95(20), 200801 (2005).
[CrossRef] [PubMed]

Kawano, S.

K. Fujita, M. Kobayashi, S. Kawano, M. Yamanaka, and S. Kawata, “High-resolution confocal microscopy by saturated excitation of fluorescence,” Phys. Rev. Lett. 99(22), 228105 (2007).
[CrossRef]

Kawata, S.

K. Fujita, M. Kobayashi, S. Kawano, M. Yamanaka, and S. Kawata, “High-resolution confocal microscopy by saturated excitation of fluorescence,” Phys. Rev. Lett. 99(22), 228105 (2007).
[CrossRef]

Knapp, H. F.

J. T. Frohn, H. F. Knapp, and A. Stemmer, “True optical resolution beyond the Rayleigh limit achieved by standing wave illumination,” Proc. Natl. Acad. Sci. U.S.A. 97(13), 7232–7236 (2000).
[CrossRef] [PubMed]

Kobayashi, M.

K. Fujita, M. Kobayashi, S. Kawano, M. Yamanaka, and S. Kawata, “High-resolution confocal microscopy by saturated excitation of fluorescence,” Phys. Rev. Lett. 99(22), 228105 (2007).
[CrossRef]

Konan, D.

G. Maire, F. Drsek, J. Girard, H. Giovannini, A. Talneau, D. Konan, K. Belkebir, P. C. Chaumet, and A. Sentenac, “Experimental demonstration of quantitative imaging beyond Abbe’s limit with optical diffraction tomography,” Phys. Rev. Lett. 102(21), 213905 (2009).
[CrossRef] [PubMed]

Kostelak, R. L.

E. Betzig, J. K. Trautman, T. D. Harris, J. S. Weiner, and R. L. Kostelak, “Breaking the diffraction barrier: optical microscopy on a nanometric scale,” Science 251(5000), 1468–1470 (1991).
[CrossRef] [PubMed]

Kuznetsova, Y.

Lacoste, T. D.

T. D. Lacoste, X. Michalet, F. Pinaud, D. S. Chemla, A. P. Alivisatos, and S. Weiss, “Ultrahigh-resolution multicolor colocalization of single fluorescent probes,” Proc. Natl. Acad. Sci. U.S.A. 97(17), 9461–9466 (2000).
[CrossRef] [PubMed]

Lindwasser, O. W.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

Lippincott-Schwartz, J.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

Lukosz, W.

Maire, G.

G. Maire, F. Drsek, J. Girard, H. Giovannini, A. Talneau, D. Konan, K. Belkebir, P. C. Chaumet, and A. Sentenac, “Experimental demonstration of quantitative imaging beyond Abbe’s limit with optical diffraction tomography,” Phys. Rev. Lett. 102(21), 213905 (2009).
[CrossRef] [PubMed]

Manstein, D. J.

D. Toomre and D. J. Manstein, “Lighting up the cell surface with evanescent wave microscopy,” Trends Cell Biol. 11(7), 298–303 (2001).
[CrossRef] [PubMed]

Mason, M. D.

S. T. Hess, T. P. K. Girirajan, and M. D. Mason, “Ultra-high resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J. 91(11), 4258–4272 (2006).
[CrossRef] [PubMed]

Michalet, X.

T. D. Lacoste, X. Michalet, F. Pinaud, D. S. Chemla, A. P. Alivisatos, and S. Weiss, “Ultrahigh-resolution multicolor colocalization of single fluorescent probes,” Proc. Natl. Acad. Sci. U.S.A. 97(17), 9461–9466 (2000).
[CrossRef] [PubMed]

Mico, V.

Z. Zalevsky, E. Fish, N. Shachar, Y. Vexberg, V. Mico, and J. Garcia, “Super-resolved imaging with randomly distributed, time- and size-varied particles,” J. Opt. A, Pure Appl. Opt. 11(8), 085406 (2009).
[CrossRef]

Z. Zalevsky, E. Saat, S. Orbach, V. Mico, and J. Garcia, “Exceeding the resolving imaging power using environmental conditions,” Appl. Opt. 47(4), A1–A6 (2008).
[CrossRef] [PubMed]

Micó, V.

Neumann, A.

Olenych, S.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

Orbach, S.

Patterson, G. H.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

Pinaud, F.

T. D. Lacoste, X. Michalet, F. Pinaud, D. S. Chemla, A. P. Alivisatos, and S. Weiss, “Ultrahigh-resolution multicolor colocalization of single fluorescent probes,” Proc. Natl. Acad. Sci. U.S.A. 97(17), 9461–9466 (2000).
[CrossRef] [PubMed]

Reddy, G. D.

O. Gliko, G. D. Reddy, B. Anvari, W. E. Brownell, and P. Saggau, “Standing wave total internal reflection fluorescence microscopy to measure the size of nanostructures in living cells,” J. Biomed. Opt. 11(6), 064013 (2006).
[CrossRef]

Rust, M. J.

M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods 3(10), 793–796 (2006).
[CrossRef] [PubMed]

Saat, E.

Saggau, P.

O. Gliko, G. D. Reddy, B. Anvari, W. E. Brownell, and P. Saggau, “Standing wave total internal reflection fluorescence microscopy to measure the size of nanostructures in living cells,” J. Biomed. Opt. 11(6), 064013 (2006).
[CrossRef]

Sampson, D. D.

S. A. Alexandrov, T. R. Hillman, T. Gutzler, and D. D. Sampson, “Synthetic aperture fourier holographic optical microscopy,” Phys. Rev. Lett. 97(16), 168102 (2006).
[CrossRef] [PubMed]

Sandoghdar, V.

T. Kalkbrenner, U. Håkanson, A. Schädle, S. Burger, C. Henkel, and V. Sandoghdar, “Optical microscopy via spectral modifications of a nanoantenna,” Phys. Rev. Lett. 95(20), 200801 (2005).
[CrossRef] [PubMed]

Schädle, A.

T. Kalkbrenner, U. Håkanson, A. Schädle, S. Burger, C. Henkel, and V. Sandoghdar, “Optical microscopy via spectral modifications of a nanoantenna,” Phys. Rev. Lett. 95(20), 200801 (2005).
[CrossRef] [PubMed]

Sedat, J. W.

L. Shao, B. Isaac, S. Uzawa, D. A. Agard, J. W. Sedat, and M. G. L. Gustafsson, “I5S: wide-field light microscopy with 100-nm-scale resolution in three dimensions,” Biophys. J. 94(12), 4971–4983 (2008).
[CrossRef] [PubMed]

M. G. L. Gustafsson, D. A. Agard, and J. W. Sedat, “I5M: 3D widefield light microscopy with better than 100 nm axial resolution,” J. Microsc. 195(1), 10–16 (1999).
[CrossRef] [PubMed]

Sentenac, A.

G. Maire, F. Drsek, J. Girard, H. Giovannini, A. Talneau, D. Konan, K. Belkebir, P. C. Chaumet, and A. Sentenac, “Experimental demonstration of quantitative imaging beyond Abbe’s limit with optical diffraction tomography,” Phys. Rev. Lett. 102(21), 213905 (2009).
[CrossRef] [PubMed]

Shachar, N.

Z. Zalevsky, E. Fish, N. Shachar, Y. Vexberg, V. Mico, and J. Garcia, “Super-resolved imaging with randomly distributed, time- and size-varied particles,” J. Opt. A, Pure Appl. Opt. 11(8), 085406 (2009).
[CrossRef]

Shao, L.

L. Shao, B. Isaac, S. Uzawa, D. A. Agard, J. W. Sedat, and M. G. L. Gustafsson, “I5S: wide-field light microscopy with 100-nm-scale resolution in three dimensions,” Biophys. J. 94(12), 4971–4983 (2008).
[CrossRef] [PubMed]

Sheppard, C. J. R.

Sougrat, R.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

Stelzer, E. H. K.

S. Hell and E. H. K. Stelzer, “Fundamental improvement of resolution with a 4Pi-confocal fluorescence microscope using two-photon excitation,” Opt. Commun. 93(5-6), 277–282 (1992).
[CrossRef]

Stemmer, A.

R. Fiolka, M. Beck, and A. Stemmer, “Structured illumination in total internal reflection fluorescence microscopy using a spatial light modulator,” Opt. Lett. 33(14), 1629–1631 (2008).
[CrossRef] [PubMed]

J. T. Frohn, H. F. Knapp, and A. Stemmer, “True optical resolution beyond the Rayleigh limit achieved by standing wave illumination,” Proc. Natl. Acad. Sci. U.S.A. 97(13), 7232–7236 (2000).
[CrossRef] [PubMed]

Talneau, A.

G. Maire, F. Drsek, J. Girard, H. Giovannini, A. Talneau, D. Konan, K. Belkebir, P. C. Chaumet, and A. Sentenac, “Experimental demonstration of quantitative imaging beyond Abbe’s limit with optical diffraction tomography,” Phys. Rev. Lett. 102(21), 213905 (2009).
[CrossRef] [PubMed]

Toomre, D.

D. Toomre and D. J. Manstein, “Lighting up the cell surface with evanescent wave microscopy,” Trends Cell Biol. 11(7), 298–303 (2001).
[CrossRef] [PubMed]

Trautman, J. K.

E. Betzig, J. K. Trautman, T. D. Harris, J. S. Weiner, and R. L. Kostelak, “Breaking the diffraction barrier: optical microscopy on a nanometric scale,” Science 251(5000), 1468–1470 (1991).
[CrossRef] [PubMed]

Uzawa, S.

L. Shao, B. Isaac, S. Uzawa, D. A. Agard, J. W. Sedat, and M. G. L. Gustafsson, “I5S: wide-field light microscopy with 100-nm-scale resolution in three dimensions,” Biophys. J. 94(12), 4971–4983 (2008).
[CrossRef] [PubMed]

Vermolen, B. J.

Y. Garini, B. J. Vermolen, and I. T. Young, “From micro to nano: recent advances in high-resolution microscopy,” Curr. Opin. Biotechnol. 16(1), 3–12 (2005).
[CrossRef] [PubMed]

Vexberg, Y.

Z. Zalevsky, E. Fish, N. Shachar, Y. Vexberg, V. Mico, and J. Garcia, “Super-resolved imaging with randomly distributed, time- and size-varied particles,” J. Opt. A, Pure Appl. Opt. 11(8), 085406 (2009).
[CrossRef]

Webb, W. W.

W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21(11), 1369–1377 (2003).
[CrossRef] [PubMed]

Weiner, J. S.

E. Betzig, J. K. Trautman, T. D. Harris, J. S. Weiner, and R. L. Kostelak, “Breaking the diffraction barrier: optical microscopy on a nanometric scale,” Science 251(5000), 1468–1470 (1991).
[CrossRef] [PubMed]

Weiss, S.

T. D. Lacoste, X. Michalet, F. Pinaud, D. S. Chemla, A. P. Alivisatos, and S. Weiss, “Ultrahigh-resolution multicolor colocalization of single fluorescent probes,” Proc. Natl. Acad. Sci. U.S.A. 97(17), 9461–9466 (2000).
[CrossRef] [PubMed]

Westphal, V.

V. Westphal and S. W. Hell, “Nanoscale resolution in the focal plane of an optical microscope,” Phys. Rev. Lett. 94(14), 143903 (2005).
[CrossRef] [PubMed]

Wichmann, J.

Williams, R. M.

W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21(11), 1369–1377 (2003).
[CrossRef] [PubMed]

Yamanaka, M.

K. Fujita, M. Kobayashi, S. Kawano, M. Yamanaka, and S. Kawata, “High-resolution confocal microscopy by saturated excitation of fluorescence,” Phys. Rev. Lett. 99(22), 228105 (2007).
[CrossRef]

Young, I. T.

Y. Garini, B. J. Vermolen, and I. T. Young, “From micro to nano: recent advances in high-resolution microscopy,” Curr. Opin. Biotechnol. 16(1), 3–12 (2005).
[CrossRef] [PubMed]

Zalevsky, Z.

Zhuang, X.

M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods 3(10), 793–796 (2006).
[CrossRef] [PubMed]

Zipfel, W. R.

W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21(11), 1369–1377 (2003).
[CrossRef] [PubMed]

Appl. Opt. (1)

Archiv für Mikroskopische Anatomie (1)

E. Abbe, “Beiträge zur Theorie des Mikroskops und der mikroskopischen Wahrnehmung,” Archiv für Mikroskopische Anatomie 9(1), 413–418 (1873).
[CrossRef]

Biophys. J. (2)

S. T. Hess, T. P. K. Girirajan, and M. D. Mason, “Ultra-high resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J. 91(11), 4258–4272 (2006).
[CrossRef] [PubMed]

L. Shao, B. Isaac, S. Uzawa, D. A. Agard, J. W. Sedat, and M. G. L. Gustafsson, “I5S: wide-field light microscopy with 100-nm-scale resolution in three dimensions,” Biophys. J. 94(12), 4971–4983 (2008).
[CrossRef] [PubMed]

Curr. Opin. Biotechnol. (1)

Y. Garini, B. J. Vermolen, and I. T. Young, “From micro to nano: recent advances in high-resolution microscopy,” Curr. Opin. Biotechnol. 16(1), 3–12 (2005).
[CrossRef] [PubMed]

J. Biomed. Opt. (1)

O. Gliko, G. D. Reddy, B. Anvari, W. E. Brownell, and P. Saggau, “Standing wave total internal reflection fluorescence microscopy to measure the size of nanostructures in living cells,” J. Biomed. Opt. 11(6), 064013 (2006).
[CrossRef]

J. Microsc. (2)

M. G. L. Gustafsson, D. A. Agard, and J. W. Sedat, “I5M: 3D widefield light microscopy with better than 100 nm axial resolution,” J. Microsc. 195(1), 10–16 (1999).
[CrossRef] [PubMed]

M. G. L. Gustafsson, “Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy,” J. Microsc. 198(2), 82–87 (2000).
[CrossRef] [PubMed]

J. Opt. A, Pure Appl. Opt. (1)

Z. Zalevsky, E. Fish, N. Shachar, Y. Vexberg, V. Mico, and J. Garcia, “Super-resolved imaging with randomly distributed, time- and size-varied particles,” J. Opt. A, Pure Appl. Opt. 11(8), 085406 (2009).
[CrossRef]

J. Opt. Soc. Am. (1)

J. Opt. Soc. Am. A (1)

Nat. Biotechnol. (1)

W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21(11), 1369–1377 (2003).
[CrossRef] [PubMed]

Nat. Methods (1)

M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods 3(10), 793–796 (2006).
[CrossRef] [PubMed]

Opt. Commun. (1)

S. Hell and E. H. K. Stelzer, “Fundamental improvement of resolution with a 4Pi-confocal fluorescence microscope using two-photon excitation,” Opt. Commun. 93(5-6), 277–282 (1992).
[CrossRef]

Opt. Express (2)

Opt. Lett. (2)

Phys. Rev. Lett. (7)

S. A. Alexandrov, T. R. Hillman, T. Gutzler, and D. D. Sampson, “Synthetic aperture fourier holographic optical microscopy,” Phys. Rev. Lett. 97(16), 168102 (2006).
[CrossRef] [PubMed]

E. Baleine and A. Dogariu, “Variable coherence scattering microscopy,” Phys. Rev. Lett. 95(19), 193904 (2005).
[CrossRef] [PubMed]

T. Kalkbrenner, U. Håkanson, A. Schädle, S. Burger, C. Henkel, and V. Sandoghdar, “Optical microscopy via spectral modifications of a nanoantenna,” Phys. Rev. Lett. 95(20), 200801 (2005).
[CrossRef] [PubMed]

G. Maire, F. Drsek, J. Girard, H. Giovannini, A. Talneau, D. Konan, K. Belkebir, P. C. Chaumet, and A. Sentenac, “Experimental demonstration of quantitative imaging beyond Abbe’s limit with optical diffraction tomography,” Phys. Rev. Lett. 102(21), 213905 (2009).
[CrossRef] [PubMed]

K. Fujita, M. Kobayashi, S. Kawano, M. Yamanaka, and S. Kawata, “High-resolution confocal microscopy by saturated excitation of fluorescence,” Phys. Rev. Lett. 99(22), 228105 (2007).
[CrossRef]

V. Westphal and S. W. Hell, “Nanoscale resolution in the focal plane of an optical microscope,” Phys. Rev. Lett. 94(14), 143903 (2005).
[CrossRef] [PubMed]

S. Bretschneider, C. Eggeling, and S. W. Hell, “Breaking the diffraction barrier in fluorescence microscopy by optical shelving,” Phys. Rev. Lett. 98(21), 218103 (2007).
[CrossRef] [PubMed]

Proc. Natl. Acad. Sci. U.S.A. (3)

T. D. Lacoste, X. Michalet, F. Pinaud, D. S. Chemla, A. P. Alivisatos, and S. Weiss, “Ultrahigh-resolution multicolor colocalization of single fluorescent probes,” Proc. Natl. Acad. Sci. U.S.A. 97(17), 9461–9466 (2000).
[CrossRef] [PubMed]

M. G. L. Gustafsson, “Nonlinear structured-illumination microscopy: wide-field fluorescence imaging with theoretically unlimited resolution,” Proc. Natl. Acad. Sci. U.S.A. 102(37), 13081–13086 (2005).
[CrossRef] [PubMed]

J. T. Frohn, H. F. Knapp, and A. Stemmer, “True optical resolution beyond the Rayleigh limit achieved by standing wave illumination,” Proc. Natl. Acad. Sci. U.S.A. 97(13), 7232–7236 (2000).
[CrossRef] [PubMed]

Science (2)

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

E. Betzig, J. K. Trautman, T. D. Harris, J. S. Weiner, and R. L. Kostelak, “Breaking the diffraction barrier: optical microscopy on a nanometric scale,” Science 251(5000), 1468–1470 (1991).
[CrossRef] [PubMed]

Trends Cell Biol. (1)

D. Toomre and D. J. Manstein, “Lighting up the cell surface with evanescent wave microscopy,” Trends Cell Biol. 11(7), 298–303 (2001).
[CrossRef] [PubMed]

Other (3)

D. Semwogerere, and E. R. Weeks, Confocal Microscopy, Encyclopedia of Biomaterials and Biomedical Engineering, (Taylor & Francis, 2005).

Z. Zalevsky, D. Mendlovic, and A. W. Lohmann, Optical systems with improved resolving power, Progress in Optics, ed., E. Wolf, (Elsevier, 2000) Vol 40, Ch. 4.

Z. Zalevsky, and D. Mendlovic, Optical Super Resolution, (Springer, New York, 2002).

Supplementary Material (1)

» Media 1: AVI (8023 KB)     

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Figures (6)

Fig. 1
Fig. 1

Experimental layout. (a) Olympus BX51 Microscope station, (b) 60X water immersion lens with NA = 1, and (c) water droplet containing gold nanoparticles flowing in proximity to the inspected sample.

Fig. 2
Fig. 2

SEM image of the fabricated resolution test target (up), and magnification of the elements S4-S5-S6 marked with a white square in the upper image (down).

Fig. 3
Fig. 3

Plot of the accumulative portion for the coverage of the field of view versus the number of frames that were in use.

Fig. 4
Fig. 4

(Media 1) (a) Diffraction limited image of the nanoparticles distribution, and (b) one diffraction limited image of the resolution target (with the particles) captured by the camera. (c). The movie presenting the flow of the nanoparticles on top of the inspected resolution target.

Fig. 5
Fig. 5

(a) Diffraction limited image of the resolution test target obtained when averaging the entire set of captured images, and (b) super resolved reconstruction containing sub-wavelength details.

Fig. 6
Fig. 6

Comparison of averaged plots of unresolved (solid line) and super resolved (dashed line) elements S4, S5 and S6 in the resolution test target.

Equations (7)

Equations on this page are rendered with MathJax. Learn more.

x ' s ( x ' ) f ( x ' , t ) p ( x x ' ) d x '
r ( x ) = t [ x ' s ( x ' ) f ( x ' , t ) p ( x x ' ) d x ' ] f ˜ ( x , t ) d t
t f ( x ' , t ) f ˜ ( x , t ) d t = 1 2 π σ exp ( ( x ' x ) 2 2 σ 2 ) + κ
lim σ 0 1 2 π σ exp ( ( x ' x ) 2 2 σ 2 ) = δ ( x ' x )
r ( x ) = x ' s ( x ' ) [ t f ˜ ( x ' , t ) f ( x , t ) d t ] p ( x x ' ) d x ' = x ' s ( x ' ) 1 2 π σ exp ( ( x ' x ) 2 2 σ 2 ) p ( x x ' ) d x ' + κ x ' s ( x ' ) p ( x x ' ) d x ' = s ( x ) [ 1 2 π σ exp ( x 2 2 σ 2 ) p ( x ) ] + κ x ' s ( x ' ) p ( x x ' ) d x '
r ( x ) = x ' s ( x ' ) [ t f ˜ ( x ' , t ) f ( x , t ) d t ] p ( x x ' ) d x ' = x ' s ( x ' ) δ ( x ' x ) p ( x x ' ) d x ' + κ x ' s ( x ' ) p ( x x ' ) d x ' = s ( x ) p ( 0 ) + κ x ' s ( x ' ) p ( x x ' ) d x '
η ( N ) = η ( N 1 ) ( 1 β ) + β                       N 2 η ( 1 ) = β

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