Abstract

We report on the application of an optically tunable coherent white light source, based on the supercontinuum generation from microstructured optical fibres, to fluorescence lifetime imaging (FLIM) microscopy and Foerster resonance energy transfer (FRET). A prototype lens based on monotonic longitudinal chromatic aberration is used for tuning the supercontinuum wavelengths from 400 to 1000 nm and acts as an axial monochromator, suitable for fibre delivery in confocal microscopy.

© 2010 OSA

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  1. J. R. Lakowicz, Principles of Fluorescence Spectroscopy, 3rd Edition (Springer, 2006).
  2. E. A. Jares-Erijman and T. M. Jovin, “Imaging molecular interactions in living cells by FRET microscopy,” Curr. Opin. Chem. Biol. 10(5), 409–416 (2006).
    [CrossRef] [PubMed]
  3. D. M. Owen, E. Auksorius, H. B. Manning, C. B. Talbot, P. A. A. de Beule, C. Dunsby, M. A. A. Neil, and P. M. W. French, “Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source,” Opt. Lett. 32(23), 3408–3410 (2007).
    [CrossRef] [PubMed]
  4. G. McConnell, “Confocal laser scanning fluorescence microscopy with a visible continuum source,” Opt. Express 12(13), 2844–2850 (2004).
    [CrossRef] [PubMed]
  5. H. N. Paulsen, K. M. Hilligsøe, J. Thøgersen, S. R. Keiding, and J. J. Larsen, “Coherent anti-Stokes Raman scattering microscopy with a photonic crystal fiber based light source,” Opt. Lett. 28(13), 1123–1125 (2003).
    [CrossRef] [PubMed]
  6. J. A. Palero, V. O. Boer, J. C. Vijverberg, H. C. Gerritsen, and H. J. C. M. Sterenborg, “Short-wavelength two-photon excitation fluorescence microscopy of tryptophan with a photonic crystal fiber based light source,” Opt. Express 13(14), 5363–5368 (2005).
    [CrossRef] [PubMed]
  7. C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004).
    [CrossRef]
  8. D. M. Grant, J. McGinty, E. J. McGhee, T. D. Bunney, D. M. Owen, C. B. Talbot, W. Zhang, S. Kumar, I. Munro, P. M. P. Lanigan, G. T. Kennedy, C. Dunsby, A. I. Magee, P. Courtney, M. Katan, M. A. A. Neil, and P. M. W. French, “High speed optically sectioned fluorescence lifetime imaging permits study of live cell signaling events,” Opt. Express 15(24), 15656–15673 (2007).
    [CrossRef] [PubMed]
  9. P. Blandin, S. Lévêque-Fort, S. Lécart, J. C. Cossec, M.-C. Potier, Z. Lenkei, F. Druon, and P. Georges, “Time-gated total internal reflection fluorescence microscopy with a supercontinuum excitation source,” Appl. Opt. 48(3), 553–559 (2009).
    [CrossRef] [PubMed]
  10. A. Gurtler, J. Gilijamse, A. Wetzels, L. Noordam, E. Sali, and M. Bellini, “Frequency selection of supercontinuum ultrashort pulses using a Fresnel zone plate,” Opt. Commun. 270(2), 336–339 (2007).
    [CrossRef]
  11. K. Shi, P. Li, S. Yin, and Z. Liu, “Chromatic confocal microscopy using supercontinuum light,” Opt. Express 12(10), 2096–2101 (2004).
    [CrossRef] [PubMed]
  12. G. McConnell, S. Poland, and J. M. Girkin, “Fast wavelength multiplexing of a white-light supercontinuum using a digital micromirror device for improved three-dimensional fluorescence microscopy,” Rev. Sci. Instrum. 77(1), 013702 (2006).
    [CrossRef]
  13. F. Quercioli, B. Tiribilli, and G. Molesini, “Optical Surface profile transducer,” Opt. Eng. 27, 135–142 (1988).
  14. B. J. Bacskai, J. Skoch, G. A. Hickey, R. Allen, and B. T. Hyman, “Fluorescence resonance energy transfer determinations using multiphoton fluorescence lifetime imaging microscopy to characterize amyloid-beta plaques,” J. Biomed. Opt. 8(3), 368–375 (2003).
    [CrossRef] [PubMed]
  15. C. Biskup, L. Kelbauskas, T. Zimmer, K. Benndorf, A. Bergmann, W. Becker, J. P. Ruppersberg, C. Stockklausner, and N. Klöcker, “Interaction of PSD-95 with potassium channels visualized by fluorescence lifetime-based resonance energy transfer imaging,” J. Biomed. Opt. 9(4), 753–759 (2004).
    [CrossRef] [PubMed]

2009 (1)

2007 (3)

2006 (2)

G. McConnell, S. Poland, and J. M. Girkin, “Fast wavelength multiplexing of a white-light supercontinuum using a digital micromirror device for improved three-dimensional fluorescence microscopy,” Rev. Sci. Instrum. 77(1), 013702 (2006).
[CrossRef]

E. A. Jares-Erijman and T. M. Jovin, “Imaging molecular interactions in living cells by FRET microscopy,” Curr. Opin. Chem. Biol. 10(5), 409–416 (2006).
[CrossRef] [PubMed]

2005 (1)

2004 (4)

C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004).
[CrossRef]

C. Biskup, L. Kelbauskas, T. Zimmer, K. Benndorf, A. Bergmann, W. Becker, J. P. Ruppersberg, C. Stockklausner, and N. Klöcker, “Interaction of PSD-95 with potassium channels visualized by fluorescence lifetime-based resonance energy transfer imaging,” J. Biomed. Opt. 9(4), 753–759 (2004).
[CrossRef] [PubMed]

K. Shi, P. Li, S. Yin, and Z. Liu, “Chromatic confocal microscopy using supercontinuum light,” Opt. Express 12(10), 2096–2101 (2004).
[CrossRef] [PubMed]

G. McConnell, “Confocal laser scanning fluorescence microscopy with a visible continuum source,” Opt. Express 12(13), 2844–2850 (2004).
[CrossRef] [PubMed]

2003 (2)

H. N. Paulsen, K. M. Hilligsøe, J. Thøgersen, S. R. Keiding, and J. J. Larsen, “Coherent anti-Stokes Raman scattering microscopy with a photonic crystal fiber based light source,” Opt. Lett. 28(13), 1123–1125 (2003).
[CrossRef] [PubMed]

B. J. Bacskai, J. Skoch, G. A. Hickey, R. Allen, and B. T. Hyman, “Fluorescence resonance energy transfer determinations using multiphoton fluorescence lifetime imaging microscopy to characterize amyloid-beta plaques,” J. Biomed. Opt. 8(3), 368–375 (2003).
[CrossRef] [PubMed]

1988 (1)

F. Quercioli, B. Tiribilli, and G. Molesini, “Optical Surface profile transducer,” Opt. Eng. 27, 135–142 (1988).

Allen, R.

B. J. Bacskai, J. Skoch, G. A. Hickey, R. Allen, and B. T. Hyman, “Fluorescence resonance energy transfer determinations using multiphoton fluorescence lifetime imaging microscopy to characterize amyloid-beta plaques,” J. Biomed. Opt. 8(3), 368–375 (2003).
[CrossRef] [PubMed]

Auksorius, E.

Bacskai, B. J.

B. J. Bacskai, J. Skoch, G. A. Hickey, R. Allen, and B. T. Hyman, “Fluorescence resonance energy transfer determinations using multiphoton fluorescence lifetime imaging microscopy to characterize amyloid-beta plaques,” J. Biomed. Opt. 8(3), 368–375 (2003).
[CrossRef] [PubMed]

Becker, W.

C. Biskup, L. Kelbauskas, T. Zimmer, K. Benndorf, A. Bergmann, W. Becker, J. P. Ruppersberg, C. Stockklausner, and N. Klöcker, “Interaction of PSD-95 with potassium channels visualized by fluorescence lifetime-based resonance energy transfer imaging,” J. Biomed. Opt. 9(4), 753–759 (2004).
[CrossRef] [PubMed]

Bellini, M.

A. Gurtler, J. Gilijamse, A. Wetzels, L. Noordam, E. Sali, and M. Bellini, “Frequency selection of supercontinuum ultrashort pulses using a Fresnel zone plate,” Opt. Commun. 270(2), 336–339 (2007).
[CrossRef]

Benndorf, K.

C. Biskup, L. Kelbauskas, T. Zimmer, K. Benndorf, A. Bergmann, W. Becker, J. P. Ruppersberg, C. Stockklausner, and N. Klöcker, “Interaction of PSD-95 with potassium channels visualized by fluorescence lifetime-based resonance energy transfer imaging,” J. Biomed. Opt. 9(4), 753–759 (2004).
[CrossRef] [PubMed]

Bergmann, A.

C. Biskup, L. Kelbauskas, T. Zimmer, K. Benndorf, A. Bergmann, W. Becker, J. P. Ruppersberg, C. Stockklausner, and N. Klöcker, “Interaction of PSD-95 with potassium channels visualized by fluorescence lifetime-based resonance energy transfer imaging,” J. Biomed. Opt. 9(4), 753–759 (2004).
[CrossRef] [PubMed]

Biskup, C.

C. Biskup, L. Kelbauskas, T. Zimmer, K. Benndorf, A. Bergmann, W. Becker, J. P. Ruppersberg, C. Stockklausner, and N. Klöcker, “Interaction of PSD-95 with potassium channels visualized by fluorescence lifetime-based resonance energy transfer imaging,” J. Biomed. Opt. 9(4), 753–759 (2004).
[CrossRef] [PubMed]

Blandin, P.

Boer, V. O.

Bunney, T. D.

Cossec, J. C.

Courtney, P.

Davis, D. M.

C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004).
[CrossRef]

de Beule, P. A. A.

Druon, F.

Dunsby, C.

Elson, D. S.

C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004).
[CrossRef]

French, P. M. W.

Galletly, N.

C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004).
[CrossRef]

Georges, P.

Gerritsen, H. C.

Gilijamse, J.

A. Gurtler, J. Gilijamse, A. Wetzels, L. Noordam, E. Sali, and M. Bellini, “Frequency selection of supercontinuum ultrashort pulses using a Fresnel zone plate,” Opt. Commun. 270(2), 336–339 (2007).
[CrossRef]

Girkin, J. M.

G. McConnell, S. Poland, and J. M. Girkin, “Fast wavelength multiplexing of a white-light supercontinuum using a digital micromirror device for improved three-dimensional fluorescence microscopy,” Rev. Sci. Instrum. 77(1), 013702 (2006).
[CrossRef]

Grant, D. M.

Gurtler, A.

A. Gurtler, J. Gilijamse, A. Wetzels, L. Noordam, E. Sali, and M. Bellini, “Frequency selection of supercontinuum ultrashort pulses using a Fresnel zone plate,” Opt. Commun. 270(2), 336–339 (2007).
[CrossRef]

Hickey, G. A.

B. J. Bacskai, J. Skoch, G. A. Hickey, R. Allen, and B. T. Hyman, “Fluorescence resonance energy transfer determinations using multiphoton fluorescence lifetime imaging microscopy to characterize amyloid-beta plaques,” J. Biomed. Opt. 8(3), 368–375 (2003).
[CrossRef] [PubMed]

Hilligsøe, K. M.

Hyman, B. T.

B. J. Bacskai, J. Skoch, G. A. Hickey, R. Allen, and B. T. Hyman, “Fluorescence resonance energy transfer determinations using multiphoton fluorescence lifetime imaging microscopy to characterize amyloid-beta plaques,” J. Biomed. Opt. 8(3), 368–375 (2003).
[CrossRef] [PubMed]

Jares-Erijman, E. A.

E. A. Jares-Erijman and T. M. Jovin, “Imaging molecular interactions in living cells by FRET microscopy,” Curr. Opin. Chem. Biol. 10(5), 409–416 (2006).
[CrossRef] [PubMed]

Jovin, T. M.

E. A. Jares-Erijman and T. M. Jovin, “Imaging molecular interactions in living cells by FRET microscopy,” Curr. Opin. Chem. Biol. 10(5), 409–416 (2006).
[CrossRef] [PubMed]

Katan, M.

Keiding, S. R.

Kelbauskas, L.

C. Biskup, L. Kelbauskas, T. Zimmer, K. Benndorf, A. Bergmann, W. Becker, J. P. Ruppersberg, C. Stockklausner, and N. Klöcker, “Interaction of PSD-95 with potassium channels visualized by fluorescence lifetime-based resonance energy transfer imaging,” J. Biomed. Opt. 9(4), 753–759 (2004).
[CrossRef] [PubMed]

Kennedy, G. T.

Klöcker, N.

C. Biskup, L. Kelbauskas, T. Zimmer, K. Benndorf, A. Bergmann, W. Becker, J. P. Ruppersberg, C. Stockklausner, and N. Klöcker, “Interaction of PSD-95 with potassium channels visualized by fluorescence lifetime-based resonance energy transfer imaging,” J. Biomed. Opt. 9(4), 753–759 (2004).
[CrossRef] [PubMed]

Kumar, S.

Lanigan, P. M. P.

D. M. Grant, J. McGinty, E. J. McGhee, T. D. Bunney, D. M. Owen, C. B. Talbot, W. Zhang, S. Kumar, I. Munro, P. M. P. Lanigan, G. T. Kennedy, C. Dunsby, A. I. Magee, P. Courtney, M. Katan, M. A. A. Neil, and P. M. W. French, “High speed optically sectioned fluorescence lifetime imaging permits study of live cell signaling events,” Opt. Express 15(24), 15656–15673 (2007).
[CrossRef] [PubMed]

C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004).
[CrossRef]

Larsen, J. J.

Lécart, S.

Lenkei, Z.

Lévêque-Fort, S.

Li, P.

Liu, Z.

Magee, A. I.

Manning, H. B.

McCann, F.

C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004).
[CrossRef]

McConnell, G.

G. McConnell, S. Poland, and J. M. Girkin, “Fast wavelength multiplexing of a white-light supercontinuum using a digital micromirror device for improved three-dimensional fluorescence microscopy,” Rev. Sci. Instrum. 77(1), 013702 (2006).
[CrossRef]

G. McConnell, “Confocal laser scanning fluorescence microscopy with a visible continuum source,” Opt. Express 12(13), 2844–2850 (2004).
[CrossRef] [PubMed]

McGhee, E. J.

McGinty, J.

D. M. Grant, J. McGinty, E. J. McGhee, T. D. Bunney, D. M. Owen, C. B. Talbot, W. Zhang, S. Kumar, I. Munro, P. M. P. Lanigan, G. T. Kennedy, C. Dunsby, A. I. Magee, P. Courtney, M. Katan, M. A. A. Neil, and P. M. W. French, “High speed optically sectioned fluorescence lifetime imaging permits study of live cell signaling events,” Opt. Express 15(24), 15656–15673 (2007).
[CrossRef] [PubMed]

C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004).
[CrossRef]

Molesini, G.

F. Quercioli, B. Tiribilli, and G. Molesini, “Optical Surface profile transducer,” Opt. Eng. 27, 135–142 (1988).

Munro, I.

D. M. Grant, J. McGinty, E. J. McGhee, T. D. Bunney, D. M. Owen, C. B. Talbot, W. Zhang, S. Kumar, I. Munro, P. M. P. Lanigan, G. T. Kennedy, C. Dunsby, A. I. Magee, P. Courtney, M. Katan, M. A. A. Neil, and P. M. W. French, “High speed optically sectioned fluorescence lifetime imaging permits study of live cell signaling events,” Opt. Express 15(24), 15656–15673 (2007).
[CrossRef] [PubMed]

C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004).
[CrossRef]

Neil, M. A. A.

Noordam, L.

A. Gurtler, J. Gilijamse, A. Wetzels, L. Noordam, E. Sali, and M. Bellini, “Frequency selection of supercontinuum ultrashort pulses using a Fresnel zone plate,” Opt. Commun. 270(2), 336–339 (2007).
[CrossRef]

Onfelt, B.

C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004).
[CrossRef]

Owen, D. M.

Palero, J. A.

Paulsen, H. N.

Poland, S.

G. McConnell, S. Poland, and J. M. Girkin, “Fast wavelength multiplexing of a white-light supercontinuum using a digital micromirror device for improved three-dimensional fluorescence microscopy,” Rev. Sci. Instrum. 77(1), 013702 (2006).
[CrossRef]

Potier, M.-C.

Quercioli, F.

F. Quercioli, B. Tiribilli, and G. Molesini, “Optical Surface profile transducer,” Opt. Eng. 27, 135–142 (1988).

Requejo-Isidro, J.

C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004).
[CrossRef]

Ruppersberg, J. P.

C. Biskup, L. Kelbauskas, T. Zimmer, K. Benndorf, A. Bergmann, W. Becker, J. P. Ruppersberg, C. Stockklausner, and N. Klöcker, “Interaction of PSD-95 with potassium channels visualized by fluorescence lifetime-based resonance energy transfer imaging,” J. Biomed. Opt. 9(4), 753–759 (2004).
[CrossRef] [PubMed]

Sali, E.

A. Gurtler, J. Gilijamse, A. Wetzels, L. Noordam, E. Sali, and M. Bellini, “Frequency selection of supercontinuum ultrashort pulses using a Fresnel zone plate,” Opt. Commun. 270(2), 336–339 (2007).
[CrossRef]

Shi, K.

Skoch, J.

B. J. Bacskai, J. Skoch, G. A. Hickey, R. Allen, and B. T. Hyman, “Fluorescence resonance energy transfer determinations using multiphoton fluorescence lifetime imaging microscopy to characterize amyloid-beta plaques,” J. Biomed. Opt. 8(3), 368–375 (2003).
[CrossRef] [PubMed]

Sterenborg, H. J. C. M.

Stockklausner, C.

C. Biskup, L. Kelbauskas, T. Zimmer, K. Benndorf, A. Bergmann, W. Becker, J. P. Ruppersberg, C. Stockklausner, and N. Klöcker, “Interaction of PSD-95 with potassium channels visualized by fluorescence lifetime-based resonance energy transfer imaging,” J. Biomed. Opt. 9(4), 753–759 (2004).
[CrossRef] [PubMed]

Talbot, C. B.

Thøgersen, J.

Tiribilli, B.

F. Quercioli, B. Tiribilli, and G. Molesini, “Optical Surface profile transducer,” Opt. Eng. 27, 135–142 (1988).

Treanor, B.

C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004).
[CrossRef]

Vijverberg, J. C.

Wetzels, A.

A. Gurtler, J. Gilijamse, A. Wetzels, L. Noordam, E. Sali, and M. Bellini, “Frequency selection of supercontinuum ultrashort pulses using a Fresnel zone plate,” Opt. Commun. 270(2), 336–339 (2007).
[CrossRef]

Yin, S.

Zhang, W.

Zimmer, T.

C. Biskup, L. Kelbauskas, T. Zimmer, K. Benndorf, A. Bergmann, W. Becker, J. P. Ruppersberg, C. Stockklausner, and N. Klöcker, “Interaction of PSD-95 with potassium channels visualized by fluorescence lifetime-based resonance energy transfer imaging,” J. Biomed. Opt. 9(4), 753–759 (2004).
[CrossRef] [PubMed]

Appl. Opt. (1)

Curr. Opin. Chem. Biol. (1)

E. A. Jares-Erijman and T. M. Jovin, “Imaging molecular interactions in living cells by FRET microscopy,” Curr. Opin. Chem. Biol. 10(5), 409–416 (2006).
[CrossRef] [PubMed]

J. Biomed. Opt. (2)

B. J. Bacskai, J. Skoch, G. A. Hickey, R. Allen, and B. T. Hyman, “Fluorescence resonance energy transfer determinations using multiphoton fluorescence lifetime imaging microscopy to characterize amyloid-beta plaques,” J. Biomed. Opt. 8(3), 368–375 (2003).
[CrossRef] [PubMed]

C. Biskup, L. Kelbauskas, T. Zimmer, K. Benndorf, A. Bergmann, W. Becker, J. P. Ruppersberg, C. Stockklausner, and N. Klöcker, “Interaction of PSD-95 with potassium channels visualized by fluorescence lifetime-based resonance energy transfer imaging,” J. Biomed. Opt. 9(4), 753–759 (2004).
[CrossRef] [PubMed]

J. Phys. D Appl. Phys. (1)

C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004).
[CrossRef]

Opt. Commun. (1)

A. Gurtler, J. Gilijamse, A. Wetzels, L. Noordam, E. Sali, and M. Bellini, “Frequency selection of supercontinuum ultrashort pulses using a Fresnel zone plate,” Opt. Commun. 270(2), 336–339 (2007).
[CrossRef]

Opt. Eng. (1)

F. Quercioli, B. Tiribilli, and G. Molesini, “Optical Surface profile transducer,” Opt. Eng. 27, 135–142 (1988).

Opt. Express (4)

Opt. Lett. (2)

Rev. Sci. Instrum. (1)

G. McConnell, S. Poland, and J. M. Girkin, “Fast wavelength multiplexing of a white-light supercontinuum using a digital micromirror device for improved three-dimensional fluorescence microscopy,” Rev. Sci. Instrum. 77(1), 013702 (2006).
[CrossRef]

Other (1)

J. R. Lakowicz, Principles of Fluorescence Spectroscopy, 3rd Edition (Springer, 2006).

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Figures (5)

Fig. 1
Fig. 1

Experimental set-up.

Fig. 2
Fig. 2

SC spectrum plotted on logarithmic scale for a pumping wavelength of 800 nm. The red line shows the zero dispersion wavelength (670 nm).

Fig. 3
Fig. 3

a) Plot of the monochromator lens and ray tracing of two spectral components, red line: 1000 nm, blue line: 400 nm, made by CODE V optical design program; b) filtering action of the monochromator lens: the black line indicates the SC spectrum of the NL-1.7-670 MOF, while the color curves show the filtered spectral lines at the wavelengths listed in Table 1; c) monotonical increase of the BFL with wavelength: continuous line: calculated, red squares: experimental.

Fig. 4
Fig. 4

RGB confocal image of a single BPAE cell: blue: DAPI stained nucleus excited at 405 nm; red: MitoTracker® Red CMXRos stained mitrochondria, excited at 550 nm; green: BODIPY® FL phallacidin stained F-actin filaments, excited at 490 nm.

Fig. 5
Fig. 5

Lifetime image in false colours corresponding to lifetime values in picoseconds (left) showed in the lifetime histogram (right): a) Cell only transfected with ECFP at room temperature, b) Cell transfected with ECFP and EYFP showing FRET.

Tables (1)

Tables Icon

Table 1 Diffraction limited spot, Back Focal Length (BFL), on-axis wave rms, Strehl ratio and measured FWHM at some selected wavelengths

Metrics