Abstract

In this work we systematically explored performance of an EM-CCD as a detector for spatially resolved total internal reflection image correlation spectroscopy (TIR-ICS) with respect to adjustable parameters. We show that variations in the observation volume (pixel binning) can be well described by a simple structural term ω. To test the sensitivity of camera-based TIR-ICS we measured diffusion coefficients and particle numbers (PN) of fluorescent probes of different sizes (Fluorospheres, GFP and labeled antibodies) at varying viscosities, concentrations, and sampling rates. TIR-ICS allowed distinguishing between different probe concentrations with differences in PN of 5% and differences of 6% in D by acquiring only 15 independent measurement runs.

© 2010 OSA

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2008 (2)

J. Langowski, “Protein-protein interactions determined by fluorescence correlation spectroscopy,” Methods Cell Biol. 85, 471–484 (2008).
[CrossRef]

J. R. Unruh and E. Gratton, “Analysis of molecular concentration and brightness from fluorescence fluctuation data with an electron multiplied CCD camera,” Biophys. J. 95(11), 5385–5398 (2008).
[CrossRef]

2007 (6)

B. Kannan, L. Guo, T. Sudhaharan, S. Ahmed, I. Maruyama, and T. Wohland, “Spatially resolved total internal reflection fluorescence correlation microscopy using an electron multiplying charge-coupled device camera,” Anal. Chem. 79(12), 4463–4470 (2007).
[CrossRef]

K. Bacia and P. Schwille, “Fluorescence correlation spectroscopy,” Methods Mol. Biol. 398, 73–84 (2007).
[CrossRef]

S. A. Kim, K. G. Heinze, and P. Schwille, “Fluorescence correlation spectroscopy in living cells,” Nat. Methods 4(11), 963–973 (2007).
[CrossRef]

T. Dertinger, V. Pacheco, I. von der Hocht, R. Hartmann, I. Gregor, and J. Enderlein, “Two-focus fluorescence correlation spectroscopy: a new tool for accurate and absolute diffusion measurements,” ChemPhysChem 8(3), 433–443 (2007).
[CrossRef]

N. L. Thompson and B. L. Steele, “Total internal reflection with fluorescence correlation spectroscopy,” Nat. Protoc. 2(4), 878–890 (2007).
[CrossRef]

E. Haustein and P. Schwille, “Fluorescence correlation spectroscopy: novel variations of an established technique,” Annu. Rev. Biophys. Biomol. Struct. 36(1), 151–169 (2007).
[CrossRef]

2006 (2)

O. Kochubey, A. Majumdar, and J. Klingauf, “Imaging clathrin dynamics in Drosophila melanogaster hemocytes reveals a role for actin in vesicle fission,” Traffic 7(12), 1614–1627 (2006).
[CrossRef]

B. Kannan, J. Y. Har, P. Liu, I. Maruyama, J. L. Ding, and T. Wohland, “Electron multiplying charge-coupled device camera based fluorescence correlation spectroscopy,” Anal. Chem. 78(10), 3444–3451 (2006).
[CrossRef]

2005 (4)

K. Hassler, T. Anhut, R. Rigler, M. Goesch, and T. Lasser, “High count rates with total internal reflection fluorescence correlation spectroscopy,” Biophys J . 88(1), L01–3 (2005).
[CrossRef]

K. Hassler, M. Leutenegger, P. Rigler, R. Rao, R. Rigler, M. Gösch, and T. Lasser, “Total internal reflection fluorescence correlation spectroscopy (TIR-FCS) with low background and high count-rate per molecule,” Opt. Express 13(19), 7415–7423 (2005).
[CrossRef]

C. J. Merrifield, D. Perrais, and D. Zenisek, “Coupling between clathrin-coated-pit invagination, cortactin recruitment, and membrane scission observed in live cells,” Cell 121(4), 593–606 (2005).
[CrossRef]

D. Loerke, M. Wienisch, O. Kochubey, and J. Klingauf, “Differential control of clathrin subunit dynamics measured with EW-FRAP microscopy,” Traffic 6(10), 918–929 (2005).
[CrossRef]

2004 (1)

2001 (1)

T. E. Starr and N. L. Thompson, “Total internal reflection with fluorescence correlation spectroscopy: combined surface reaction and solution diffusion,” Biophys. J. 80(3), 1575–1584 (2001).
[CrossRef]

1999 (1)

S. E. Sund, J. A. Swanson, and D. Axelrod, “Cell membrane orientation visualized by polarized total internal reflection fluorescence,” Biophys. J. 77(4), 2266–2283 (1999).
[CrossRef]

1998 (1)

B. C. Lagerholm and N. L. Thompson, “Theory for ligand rebinding at cell membrane surfaces,” Biophys. J. 74(3), 1215–1228 (1998).
[CrossRef]

1997 (1)

P. Schwille, F. J. Meyer-Almes, and R. Rigler, “Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution,” Biophys. J. 72(4), 1878–1886 (1997).
[CrossRef]

1996 (1)

M. Eigen, “Prionics or the kinetic basis of prion diseases,” Biophys. Chem. 63(1), A1–A18 (1996).
[CrossRef]

1995 (1)

B. R. Terry, E. K. Matthews, and J. Haseloff, “Molecular characterisation of recombinant green fluorescent protein by fluorescence correlation microscopy,” Biochem. Biophys. Res. Commun. 217(1), 21–27 (1995).
[CrossRef]

1974 (2)

D Madge, E. L. Elson, and W. W. Webb, “Fluorescence correlation spectroscopy. I. Conceptual basis and theory,” Biopolymers 13(1), 1–27 (1974).
[CrossRef]

D. Magde, E. L. Elson, and W. W. Webb, “Fluorescence correlation spectroscopy. II. An experimental realization,” Biopolymers 13(1), 29–61 (1974).
[CrossRef]

Ahmed, S.

B. Kannan, L. Guo, T. Sudhaharan, S. Ahmed, I. Maruyama, and T. Wohland, “Spatially resolved total internal reflection fluorescence correlation microscopy using an electron multiplying charge-coupled device camera,” Anal. Chem. 79(12), 4463–4470 (2007).
[CrossRef]

Anhut, T.

K. Hassler, T. Anhut, R. Rigler, M. Goesch, and T. Lasser, “High count rates with total internal reflection fluorescence correlation spectroscopy,” Biophys J . 88(1), L01–3 (2005).
[CrossRef]

Axelrod, D.

S. E. Sund, J. A. Swanson, and D. Axelrod, “Cell membrane orientation visualized by polarized total internal reflection fluorescence,” Biophys. J. 77(4), 2266–2283 (1999).
[CrossRef]

Bacia, K.

K. Bacia and P. Schwille, “Fluorescence correlation spectroscopy,” Methods Mol. Biol. 398, 73–84 (2007).
[CrossRef]

Buschmann, V.

Dertinger, T.

T. Dertinger, V. Pacheco, I. von der Hocht, R. Hartmann, I. Gregor, and J. Enderlein, “Two-focus fluorescence correlation spectroscopy: a new tool for accurate and absolute diffusion measurements,” ChemPhysChem 8(3), 433–443 (2007).
[CrossRef]

Ding, J. L.

B. Kannan, J. Y. Har, P. Liu, I. Maruyama, J. L. Ding, and T. Wohland, “Electron multiplying charge-coupled device camera based fluorescence correlation spectroscopy,” Anal. Chem. 78(10), 3444–3451 (2006).
[CrossRef]

Eigen, M.

M. Eigen, “Prionics or the kinetic basis of prion diseases,” Biophys. Chem. 63(1), A1–A18 (1996).
[CrossRef]

Elson, E. L.

D. Magde, E. L. Elson, and W. W. Webb, “Fluorescence correlation spectroscopy. II. An experimental realization,” Biopolymers 13(1), 29–61 (1974).
[CrossRef]

D Madge, E. L. Elson, and W. W. Webb, “Fluorescence correlation spectroscopy. I. Conceptual basis and theory,” Biopolymers 13(1), 1–27 (1974).
[CrossRef]

Enderlein, J.

T. Dertinger, V. Pacheco, I. von der Hocht, R. Hartmann, I. Gregor, and J. Enderlein, “Two-focus fluorescence correlation spectroscopy: a new tool for accurate and absolute diffusion measurements,” ChemPhysChem 8(3), 433–443 (2007).
[CrossRef]

Goesch, M.

K. Hassler, T. Anhut, R. Rigler, M. Goesch, and T. Lasser, “High count rates with total internal reflection fluorescence correlation spectroscopy,” Biophys J . 88(1), L01–3 (2005).
[CrossRef]

Gösch, M.

Gratton, E.

J. R. Unruh and E. Gratton, “Analysis of molecular concentration and brightness from fluorescence fluctuation data with an electron multiplied CCD camera,” Biophys. J. 95(11), 5385–5398 (2008).
[CrossRef]

Gregor, I.

T. Dertinger, V. Pacheco, I. von der Hocht, R. Hartmann, I. Gregor, and J. Enderlein, “Two-focus fluorescence correlation spectroscopy: a new tool for accurate and absolute diffusion measurements,” ChemPhysChem 8(3), 433–443 (2007).
[CrossRef]

Guo, L.

B. Kannan, L. Guo, T. Sudhaharan, S. Ahmed, I. Maruyama, and T. Wohland, “Spatially resolved total internal reflection fluorescence correlation microscopy using an electron multiplying charge-coupled device camera,” Anal. Chem. 79(12), 4463–4470 (2007).
[CrossRef]

Har, J. Y.

B. Kannan, J. Y. Har, P. Liu, I. Maruyama, J. L. Ding, and T. Wohland, “Electron multiplying charge-coupled device camera based fluorescence correlation spectroscopy,” Anal. Chem. 78(10), 3444–3451 (2006).
[CrossRef]

Hartmann, R.

T. Dertinger, V. Pacheco, I. von der Hocht, R. Hartmann, I. Gregor, and J. Enderlein, “Two-focus fluorescence correlation spectroscopy: a new tool for accurate and absolute diffusion measurements,” ChemPhysChem 8(3), 433–443 (2007).
[CrossRef]

Haseloff, J.

B. R. Terry, E. K. Matthews, and J. Haseloff, “Molecular characterisation of recombinant green fluorescent protein by fluorescence correlation microscopy,” Biochem. Biophys. Res. Commun. 217(1), 21–27 (1995).
[CrossRef]

Hassler, K.

K. Hassler, M. Leutenegger, P. Rigler, R. Rao, R. Rigler, M. Gösch, and T. Lasser, “Total internal reflection fluorescence correlation spectroscopy (TIR-FCS) with low background and high count-rate per molecule,” Opt. Express 13(19), 7415–7423 (2005).
[CrossRef]

K. Hassler, T. Anhut, R. Rigler, M. Goesch, and T. Lasser, “High count rates with total internal reflection fluorescence correlation spectroscopy,” Biophys J . 88(1), L01–3 (2005).
[CrossRef]

Haustein, E.

E. Haustein and P. Schwille, “Fluorescence correlation spectroscopy: novel variations of an established technique,” Annu. Rev. Biophys. Biomol. Struct. 36(1), 151–169 (2007).
[CrossRef]

Heinze, K. G.

S. A. Kim, K. G. Heinze, and P. Schwille, “Fluorescence correlation spectroscopy in living cells,” Nat. Methods 4(11), 963–973 (2007).
[CrossRef]

Kannan, B.

B. Kannan, L. Guo, T. Sudhaharan, S. Ahmed, I. Maruyama, and T. Wohland, “Spatially resolved total internal reflection fluorescence correlation microscopy using an electron multiplying charge-coupled device camera,” Anal. Chem. 79(12), 4463–4470 (2007).
[CrossRef]

B. Kannan, J. Y. Har, P. Liu, I. Maruyama, J. L. Ding, and T. Wohland, “Electron multiplying charge-coupled device camera based fluorescence correlation spectroscopy,” Anal. Chem. 78(10), 3444–3451 (2006).
[CrossRef]

Kim, S. A.

S. A. Kim, K. G. Heinze, and P. Schwille, “Fluorescence correlation spectroscopy in living cells,” Nat. Methods 4(11), 963–973 (2007).
[CrossRef]

Klingauf, J.

O. Kochubey, A. Majumdar, and J. Klingauf, “Imaging clathrin dynamics in Drosophila melanogaster hemocytes reveals a role for actin in vesicle fission,” Traffic 7(12), 1614–1627 (2006).
[CrossRef]

D. Loerke, M. Wienisch, O. Kochubey, and J. Klingauf, “Differential control of clathrin subunit dynamics measured with EW-FRAP microscopy,” Traffic 6(10), 918–929 (2005).
[CrossRef]

Kochubey, O.

O. Kochubey, A. Majumdar, and J. Klingauf, “Imaging clathrin dynamics in Drosophila melanogaster hemocytes reveals a role for actin in vesicle fission,” Traffic 7(12), 1614–1627 (2006).
[CrossRef]

D. Loerke, M. Wienisch, O. Kochubey, and J. Klingauf, “Differential control of clathrin subunit dynamics measured with EW-FRAP microscopy,” Traffic 6(10), 918–929 (2005).
[CrossRef]

Kuricheti, K. K.

Lagerholm, B. C.

B. C. Lagerholm and N. L. Thompson, “Theory for ligand rebinding at cell membrane surfaces,” Biophys. J. 74(3), 1215–1228 (1998).
[CrossRef]

Langowski, J.

J. Langowski, “Protein-protein interactions determined by fluorescence correlation spectroscopy,” Methods Cell Biol. 85, 471–484 (2008).
[CrossRef]

Lasser, T.

K. Hassler, M. Leutenegger, P. Rigler, R. Rao, R. Rigler, M. Gösch, and T. Lasser, “Total internal reflection fluorescence correlation spectroscopy (TIR-FCS) with low background and high count-rate per molecule,” Opt. Express 13(19), 7415–7423 (2005).
[CrossRef]

K. Hassler, T. Anhut, R. Rigler, M. Goesch, and T. Lasser, “High count rates with total internal reflection fluorescence correlation spectroscopy,” Biophys J . 88(1), L01–3 (2005).
[CrossRef]

Leutenegger, M.

Liu, P.

B. Kannan, J. Y. Har, P. Liu, I. Maruyama, J. L. Ding, and T. Wohland, “Electron multiplying charge-coupled device camera based fluorescence correlation spectroscopy,” Anal. Chem. 78(10), 3444–3451 (2006).
[CrossRef]

Loerke, D.

D. Loerke, M. Wienisch, O. Kochubey, and J. Klingauf, “Differential control of clathrin subunit dynamics measured with EW-FRAP microscopy,” Traffic 6(10), 918–929 (2005).
[CrossRef]

Madge, D

D Madge, E. L. Elson, and W. W. Webb, “Fluorescence correlation spectroscopy. I. Conceptual basis and theory,” Biopolymers 13(1), 1–27 (1974).
[CrossRef]

Magde, D.

D. Magde, E. L. Elson, and W. W. Webb, “Fluorescence correlation spectroscopy. II. An experimental realization,” Biopolymers 13(1), 29–61 (1974).
[CrossRef]

Majumdar, A.

O. Kochubey, A. Majumdar, and J. Klingauf, “Imaging clathrin dynamics in Drosophila melanogaster hemocytes reveals a role for actin in vesicle fission,” Traffic 7(12), 1614–1627 (2006).
[CrossRef]

Maruyama, I.

B. Kannan, L. Guo, T. Sudhaharan, S. Ahmed, I. Maruyama, and T. Wohland, “Spatially resolved total internal reflection fluorescence correlation microscopy using an electron multiplying charge-coupled device camera,” Anal. Chem. 79(12), 4463–4470 (2007).
[CrossRef]

B. Kannan, J. Y. Har, P. Liu, I. Maruyama, J. L. Ding, and T. Wohland, “Electron multiplying charge-coupled device camera based fluorescence correlation spectroscopy,” Anal. Chem. 78(10), 3444–3451 (2006).
[CrossRef]

Matthews, E. K.

B. R. Terry, E. K. Matthews, and J. Haseloff, “Molecular characterisation of recombinant green fluorescent protein by fluorescence correlation microscopy,” Biochem. Biophys. Res. Commun. 217(1), 21–27 (1995).
[CrossRef]

Merrifield, C. J.

C. J. Merrifield, D. Perrais, and D. Zenisek, “Coupling between clathrin-coated-pit invagination, cortactin recruitment, and membrane scission observed in live cells,” Cell 121(4), 593–606 (2005).
[CrossRef]

Meyer-Almes, F. J.

P. Schwille, F. J. Meyer-Almes, and R. Rigler, “Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution,” Biophys. J. 72(4), 1878–1886 (1997).
[CrossRef]

Pacheco, V.

T. Dertinger, V. Pacheco, I. von der Hocht, R. Hartmann, I. Gregor, and J. Enderlein, “Two-focus fluorescence correlation spectroscopy: a new tool for accurate and absolute diffusion measurements,” ChemPhysChem 8(3), 433–443 (2007).
[CrossRef]

Perrais, D.

C. J. Merrifield, D. Perrais, and D. Zenisek, “Coupling between clathrin-coated-pit invagination, cortactin recruitment, and membrane scission observed in live cells,” Cell 121(4), 593–606 (2005).
[CrossRef]

Rao, R.

Rigler, P.

Rigler, R.

K. Hassler, M. Leutenegger, P. Rigler, R. Rao, R. Rigler, M. Gösch, and T. Lasser, “Total internal reflection fluorescence correlation spectroscopy (TIR-FCS) with low background and high count-rate per molecule,” Opt. Express 13(19), 7415–7423 (2005).
[CrossRef]

K. Hassler, T. Anhut, R. Rigler, M. Goesch, and T. Lasser, “High count rates with total internal reflection fluorescence correlation spectroscopy,” Biophys J . 88(1), L01–3 (2005).
[CrossRef]

P. Schwille, F. J. Meyer-Almes, and R. Rigler, “Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution,” Biophys. J. 72(4), 1878–1886 (1997).
[CrossRef]

Schwille, P.

S. A. Kim, K. G. Heinze, and P. Schwille, “Fluorescence correlation spectroscopy in living cells,” Nat. Methods 4(11), 963–973 (2007).
[CrossRef]

E. Haustein and P. Schwille, “Fluorescence correlation spectroscopy: novel variations of an established technique,” Annu. Rev. Biophys. Biomol. Struct. 36(1), 151–169 (2007).
[CrossRef]

K. Bacia and P. Schwille, “Fluorescence correlation spectroscopy,” Methods Mol. Biol. 398, 73–84 (2007).
[CrossRef]

P. Schwille, F. J. Meyer-Almes, and R. Rigler, “Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution,” Biophys. J. 72(4), 1878–1886 (1997).
[CrossRef]

Starr, T. E.

T. E. Starr and N. L. Thompson, “Total internal reflection with fluorescence correlation spectroscopy: combined surface reaction and solution diffusion,” Biophys. J. 80(3), 1575–1584 (2001).
[CrossRef]

Steele, B. L.

N. L. Thompson and B. L. Steele, “Total internal reflection with fluorescence correlation spectroscopy,” Nat. Protoc. 2(4), 878–890 (2007).
[CrossRef]

Sudhaharan, T.

B. Kannan, L. Guo, T. Sudhaharan, S. Ahmed, I. Maruyama, and T. Wohland, “Spatially resolved total internal reflection fluorescence correlation microscopy using an electron multiplying charge-coupled device camera,” Anal. Chem. 79(12), 4463–4470 (2007).
[CrossRef]

Sund, S. E.

S. E. Sund, J. A. Swanson, and D. Axelrod, “Cell membrane orientation visualized by polarized total internal reflection fluorescence,” Biophys. J. 77(4), 2266–2283 (1999).
[CrossRef]

Swanson, J. A.

S. E. Sund, J. A. Swanson, and D. Axelrod, “Cell membrane orientation visualized by polarized total internal reflection fluorescence,” Biophys. J. 77(4), 2266–2283 (1999).
[CrossRef]

Terry, B. R.

B. R. Terry, E. K. Matthews, and J. Haseloff, “Molecular characterisation of recombinant green fluorescent protein by fluorescence correlation microscopy,” Biochem. Biophys. Res. Commun. 217(1), 21–27 (1995).
[CrossRef]

Thompson, N. L.

N. L. Thompson and B. L. Steele, “Total internal reflection with fluorescence correlation spectroscopy,” Nat. Protoc. 2(4), 878–890 (2007).
[CrossRef]

T. E. Starr and N. L. Thompson, “Total internal reflection with fluorescence correlation spectroscopy: combined surface reaction and solution diffusion,” Biophys. J. 80(3), 1575–1584 (2001).
[CrossRef]

B. C. Lagerholm and N. L. Thompson, “Theory for ligand rebinding at cell membrane surfaces,” Biophys. J. 74(3), 1215–1228 (1998).
[CrossRef]

Unruh, J. R.

J. R. Unruh and E. Gratton, “Analysis of molecular concentration and brightness from fluorescence fluctuation data with an electron multiplied CCD camera,” Biophys. J. 95(11), 5385–5398 (2008).
[CrossRef]

von der Hocht, I.

T. Dertinger, V. Pacheco, I. von der Hocht, R. Hartmann, I. Gregor, and J. Enderlein, “Two-focus fluorescence correlation spectroscopy: a new tool for accurate and absolute diffusion measurements,” ChemPhysChem 8(3), 433–443 (2007).
[CrossRef]

Webb, W. W.

D. Magde, E. L. Elson, and W. W. Webb, “Fluorescence correlation spectroscopy. II. An experimental realization,” Biopolymers 13(1), 29–61 (1974).
[CrossRef]

D Madge, E. L. Elson, and W. W. Webb, “Fluorescence correlation spectroscopy. I. Conceptual basis and theory,” Biopolymers 13(1), 1–27 (1974).
[CrossRef]

Weston,, K. D.

Wienisch, M.

D. Loerke, M. Wienisch, O. Kochubey, and J. Klingauf, “Differential control of clathrin subunit dynamics measured with EW-FRAP microscopy,” Traffic 6(10), 918–929 (2005).
[CrossRef]

Wohland, T.

B. Kannan, L. Guo, T. Sudhaharan, S. Ahmed, I. Maruyama, and T. Wohland, “Spatially resolved total internal reflection fluorescence correlation microscopy using an electron multiplying charge-coupled device camera,” Anal. Chem. 79(12), 4463–4470 (2007).
[CrossRef]

B. Kannan, J. Y. Har, P. Liu, I. Maruyama, J. L. Ding, and T. Wohland, “Electron multiplying charge-coupled device camera based fluorescence correlation spectroscopy,” Anal. Chem. 78(10), 3444–3451 (2006).
[CrossRef]

Zenisek, D.

C. J. Merrifield, D. Perrais, and D. Zenisek, “Coupling between clathrin-coated-pit invagination, cortactin recruitment, and membrane scission observed in live cells,” Cell 121(4), 593–606 (2005).
[CrossRef]

Anal. Chem. (2)

B. Kannan, J. Y. Har, P. Liu, I. Maruyama, J. L. Ding, and T. Wohland, “Electron multiplying charge-coupled device camera based fluorescence correlation spectroscopy,” Anal. Chem. 78(10), 3444–3451 (2006).
[CrossRef]

B. Kannan, L. Guo, T. Sudhaharan, S. Ahmed, I. Maruyama, and T. Wohland, “Spatially resolved total internal reflection fluorescence correlation microscopy using an electron multiplying charge-coupled device camera,” Anal. Chem. 79(12), 4463–4470 (2007).
[CrossRef]

Annu. Rev. Biophys. Biomol. Struct. (1)

E. Haustein and P. Schwille, “Fluorescence correlation spectroscopy: novel variations of an established technique,” Annu. Rev. Biophys. Biomol. Struct. 36(1), 151–169 (2007).
[CrossRef]

Appl. Spectrosc. (1)

Biochem. Biophys. Res. Commun. (1)

B. R. Terry, E. K. Matthews, and J. Haseloff, “Molecular characterisation of recombinant green fluorescent protein by fluorescence correlation microscopy,” Biochem. Biophys. Res. Commun. 217(1), 21–27 (1995).
[CrossRef]

Biophys J (1)

K. Hassler, T. Anhut, R. Rigler, M. Goesch, and T. Lasser, “High count rates with total internal reflection fluorescence correlation spectroscopy,” Biophys J . 88(1), L01–3 (2005).
[CrossRef]

Biophys. Chem. (1)

M. Eigen, “Prionics or the kinetic basis of prion diseases,” Biophys. Chem. 63(1), A1–A18 (1996).
[CrossRef]

Biophys. J. (5)

T. E. Starr and N. L. Thompson, “Total internal reflection with fluorescence correlation spectroscopy: combined surface reaction and solution diffusion,” Biophys. J. 80(3), 1575–1584 (2001).
[CrossRef]

P. Schwille, F. J. Meyer-Almes, and R. Rigler, “Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution,” Biophys. J. 72(4), 1878–1886 (1997).
[CrossRef]

B. C. Lagerholm and N. L. Thompson, “Theory for ligand rebinding at cell membrane surfaces,” Biophys. J. 74(3), 1215–1228 (1998).
[CrossRef]

J. R. Unruh and E. Gratton, “Analysis of molecular concentration and brightness from fluorescence fluctuation data with an electron multiplied CCD camera,” Biophys. J. 95(11), 5385–5398 (2008).
[CrossRef]

S. E. Sund, J. A. Swanson, and D. Axelrod, “Cell membrane orientation visualized by polarized total internal reflection fluorescence,” Biophys. J. 77(4), 2266–2283 (1999).
[CrossRef]

Biopolymers (2)

D Madge, E. L. Elson, and W. W. Webb, “Fluorescence correlation spectroscopy. I. Conceptual basis and theory,” Biopolymers 13(1), 1–27 (1974).
[CrossRef]

D. Magde, E. L. Elson, and W. W. Webb, “Fluorescence correlation spectroscopy. II. An experimental realization,” Biopolymers 13(1), 29–61 (1974).
[CrossRef]

Cell (1)

C. J. Merrifield, D. Perrais, and D. Zenisek, “Coupling between clathrin-coated-pit invagination, cortactin recruitment, and membrane scission observed in live cells,” Cell 121(4), 593–606 (2005).
[CrossRef]

ChemPhysChem (1)

T. Dertinger, V. Pacheco, I. von der Hocht, R. Hartmann, I. Gregor, and J. Enderlein, “Two-focus fluorescence correlation spectroscopy: a new tool for accurate and absolute diffusion measurements,” ChemPhysChem 8(3), 433–443 (2007).
[CrossRef]

Methods Cell Biol. (1)

J. Langowski, “Protein-protein interactions determined by fluorescence correlation spectroscopy,” Methods Cell Biol. 85, 471–484 (2008).
[CrossRef]

Methods Mol. Biol. (1)

K. Bacia and P. Schwille, “Fluorescence correlation spectroscopy,” Methods Mol. Biol. 398, 73–84 (2007).
[CrossRef]

Nat. Methods (1)

S. A. Kim, K. G. Heinze, and P. Schwille, “Fluorescence correlation spectroscopy in living cells,” Nat. Methods 4(11), 963–973 (2007).
[CrossRef]

Nat. Protoc. (1)

N. L. Thompson and B. L. Steele, “Total internal reflection with fluorescence correlation spectroscopy,” Nat. Protoc. 2(4), 878–890 (2007).
[CrossRef]

Opt. Express (1)

Traffic (2)

D. Loerke, M. Wienisch, O. Kochubey, and J. Klingauf, “Differential control of clathrin subunit dynamics measured with EW-FRAP microscopy,” Traffic 6(10), 918–929 (2005).
[CrossRef]

O. Kochubey, A. Majumdar, and J. Klingauf, “Imaging clathrin dynamics in Drosophila melanogaster hemocytes reveals a role for actin in vesicle fission,” Traffic 7(12), 1614–1627 (2006).
[CrossRef]

Other (2)

N. L. Thompson, Fluorescence Correlation Spectroscopy, in Topics in Fluorescence Spectroscopy, (Plenum Press, New York, 1991).

A. Stroebel, O. Welzel, J. Kornhuber, and T. W. Groemer, “Background determination-based detection of scattered peaks,” Microsc. Res. Tech.; published online (2010).

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