Abstract

We investigated the possibility of using standard commercial multimode fibers (MMF), Corning SMF28 fibers, to deliver picosecond excitation lasers for coherent anti-Stokes Raman scattering (CARS) imaging. We theoretically and/or experimentally analyzed issues associated with the fiber delivery, such as dispersion length, walk-off length, nonlinear length, average threshold power for self-phase modulations, and four-wave mixing (FWM). These analyses can also be applied to other types of fibers. We found that FWM signals are generated in MMF, but they can be filtered out using a long-pass filter for CARS imaging. Finally, we demonstrated that MMF can be used for delivery of picosecond excitation lasers in the CARS imaging system without any degradation of image quality.

© 2010 OSA

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  1. J. X. Cheng and X. S. Xie, “Coherent anti-Stokes Raman scattering microscopy: instrumentation, theory, and applications,” J. Phys. Chem. B 108(3), 827–840 (2004).
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    [CrossRef] [PubMed]
  3. C. L. Evans, E. O. Potma, M. Puoris’haag, D. Côté, C. P. Lin, and X. S. Xie, “Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy,” Proc. Natl. Acad. Sci. U.S.A. 102(46), 16807–16812 (2005).
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  6. M. Müller and A. Zumbusch, “Coherent anti-Stokes Raman scattering microscopy,” ChemPhysChem 8(15), 2156–2170 (2007).
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    [CrossRef] [PubMed]
  8. J.-X. Cheng, A. Volkmer, L. D. Book, and X. S. Xie, “Multiplex coherent anti-Stokes Raman scattering microspectroscopy and study of lipid vesicles,” J. Phys. Chem. B 106(34), 8493–8498 (2002).
    [CrossRef]
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  15. E. Laemmel, M. Genet, G. Le Goualher, A. Perchant, J. F. Le Gargasson, and E. Vicaut, “Fibered confocal fluorescence microscopy (Cell-viZio) facilitates extended imaging in the field of microcirculation. A comparison with intravital microscopy,” J. Vasc. Res. 41(5), 400–411 (2004).
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2010 (1)

2009 (1)

A. Downes, R. Mouras, and A. Elfick, “A versatile CARS microscope for biological imaging,” J. Raman Spectrosc. 40(7), 757–762 (2009).
[CrossRef]

2008 (1)

C. L. Evans and X. S. Xie, “Coherent Anti-Stokes Raman Scattering Microscopy: Chemical Imaging for Biology and Medicine,” Ann. Rev. Anal. Chem. 1(1), 883–909 (2008).
[CrossRef]

2007 (3)

J. X. Cheng, “Coherent anti-stokes Raman scattering microscopy,” Appl. Spectrosc. 61(9), 197–208 (2007).
[CrossRef] [PubMed]

M. Müller and A. Zumbusch, “Coherent anti-Stokes Raman scattering microscopy,” ChemPhysChem 8(15), 2156–2170 (2007).
[CrossRef] [PubMed]

C. L. Evans, X. Xu, S. Kesari, X. S. Xie, S. T. Wong, and G. S. Young, “Chemically-selective imaging of brain structures with CARS microscopy,” Opt. Express 15(19), 12076–12087 (2007).
[CrossRef] [PubMed]

2006 (3)

2005 (4)

C. L. Evans, E. O. Potma, M. Puoris’haag, D. Côté, C. P. Lin, and X. S. Xie, “Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy,” Proc. Natl. Acad. Sci. U.S.A. 102(46), 16807–16812 (2005).
[CrossRef] [PubMed]

U. Sharma, N. M. Fried, and J. U. Kang, “All-fiber common-path optical coherence tomography: sensitivity optimization and system analysis,” IEEE J. Sel. Top. Quantum Electron. 11(4), 799–805 (2005).
[CrossRef]

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, “Fiber-optic fluorescence imaging,” Nat. Methods 2(12), 941–950 (2005).
[CrossRef] [PubMed]

Z. Wang, J. R. Heflin, R. Stolen, and S. Ramachandran, “Analysis of optical response of long period fiber gratings to nm-thick thin-film coating,” Opt. Express 13(8), 2808–2813 (2005).
[CrossRef] [PubMed]

2004 (2)

J. X. Cheng and X. S. Xie, “Coherent anti-Stokes Raman scattering microscopy: instrumentation, theory, and applications,” J. Phys. Chem. B 108(3), 827–840 (2004).
[CrossRef]

E. Laemmel, M. Genet, G. Le Goualher, A. Perchant, J. F. Le Gargasson, and E. Vicaut, “Fibered confocal fluorescence microscopy (Cell-viZio) facilitates extended imaging in the field of microcirculation. A comparison with intravital microscopy,” J. Vasc. Res. 41(5), 400–411 (2004).
[CrossRef] [PubMed]

2003 (1)

X. Nan, J. X. Cheng, and X. S. Xie, “Vibrational imaging of lipid droplets in live fibroblast cells with coherent anti-Stokes Raman scattering microscopy,” J. Lipid Res. 44(11), 2202–2208 (2003).
[CrossRef] [PubMed]

2002 (2)

J.-X. Cheng, A. Volkmer, L. D. Book, and X. S. Xie, “Multiplex coherent anti-Stokes Raman scattering microspectroscopy and study of lipid vesicles,” J. Phys. Chem. B 106(34), 8493–8498 (2002).
[CrossRef]

M. Müller and J. M. Schins, “Imaging the Thermodynamic State of Lipid Membranes with Multiplex CARS Microscopy,” J. Phys. Chem. B 106(14), 3715–3723 (2002).
[CrossRef]

1999 (1)

1997 (1)

G. J. Tearney, M. E. Brezinski, B. E. Bouma, S. A. Boppart, C. Pitris, J. F. Southern, and J. G. Fujimoto, “In vivo endoscopic optical biopsy with optical coherence tomography,” Science 276(5321), 2037–2039 (1997).
[CrossRef] [PubMed]

1992 (1)

K. Inoue, “Four-wave mixing in an optical fiber in the zero-dispersion wavelength region,” J. Lightwave Technol. 10(11), 1553–1561 (1992).
[CrossRef]

1987 (1)

P. L. Baldeck and R. R. Alfano, “Intensity effects on the stimulated four photon spectra generated by picosecond pulses in optical fibers,” J. Lightwave Technol. 5(12), 1712–1715 (1987).
[CrossRef]

1975 (1)

R. H. Stolen, “Phase-matched-stimulated four-photon mixing in silica-fiber waveguides,” IEEE J. Quantum Electron. 11(3), 100–103 (1975).
[CrossRef]

Alfano, R. R.

P. L. Baldeck and R. R. Alfano, “Intensity effects on the stimulated four photon spectra generated by picosecond pulses in optical fibers,” J. Lightwave Technol. 5(12), 1712–1715 (1987).
[CrossRef]

Baldeck, P. L.

P. L. Baldeck and R. R. Alfano, “Intensity effects on the stimulated four photon spectra generated by picosecond pulses in optical fibers,” J. Lightwave Technol. 5(12), 1712–1715 (1987).
[CrossRef]

Balu, M.

Book, L. D.

J.-X. Cheng, A. Volkmer, L. D. Book, and X. S. Xie, “Multiplex coherent anti-Stokes Raman scattering microspectroscopy and study of lipid vesicles,” J. Phys. Chem. B 106(34), 8493–8498 (2002).
[CrossRef]

Boppart, S. A.

G. J. Tearney, M. E. Brezinski, B. E. Bouma, S. A. Boppart, C. Pitris, J. F. Southern, and J. G. Fujimoto, “In vivo endoscopic optical biopsy with optical coherence tomography,” Science 276(5321), 2037–2039 (1997).
[CrossRef] [PubMed]

Bouma, B. E.

G. J. Tearney, M. E. Brezinski, B. E. Bouma, S. A. Boppart, C. Pitris, J. F. Southern, and J. G. Fujimoto, “In vivo endoscopic optical biopsy with optical coherence tomography,” Science 276(5321), 2037–2039 (1997).
[CrossRef] [PubMed]

Brezinski, M. E.

G. J. Tearney, M. E. Brezinski, B. E. Bouma, S. A. Boppart, C. Pitris, J. F. Southern, and J. G. Fujimoto, “In vivo endoscopic optical biopsy with optical coherence tomography,” Science 276(5321), 2037–2039 (1997).
[CrossRef] [PubMed]

Chen, Z.

Cheng, J. X.

J. X. Cheng, “Coherent anti-stokes Raman scattering microscopy,” Appl. Spectrosc. 61(9), 197–208 (2007).
[CrossRef] [PubMed]

J. X. Cheng and X. S. Xie, “Coherent anti-Stokes Raman scattering microscopy: instrumentation, theory, and applications,” J. Phys. Chem. B 108(3), 827–840 (2004).
[CrossRef]

X. Nan, J. X. Cheng, and X. S. Xie, “Vibrational imaging of lipid droplets in live fibroblast cells with coherent anti-Stokes Raman scattering microscopy,” J. Lipid Res. 44(11), 2202–2208 (2003).
[CrossRef] [PubMed]

Cheng, J.-X.

H. Wang, T. B. Huff, and J.-X. Cheng, “Coherent anti-Stokes Raman scattering imaging with a laser source delivered by a photonic crystal fiber,” Opt. Lett. 31(10), 1417–1419 (2006).
[CrossRef] [PubMed]

J.-X. Cheng, A. Volkmer, L. D. Book, and X. S. Xie, “Multiplex coherent anti-Stokes Raman scattering microspectroscopy and study of lipid vesicles,” J. Phys. Chem. B 106(34), 8493–8498 (2002).
[CrossRef]

Cheung, E. L. M.

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, “Fiber-optic fluorescence imaging,” Nat. Methods 2(12), 941–950 (2005).
[CrossRef] [PubMed]

Cocker, E. D.

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, “Fiber-optic fluorescence imaging,” Nat. Methods 2(12), 941–950 (2005).
[CrossRef] [PubMed]

Côté, D.

C. L. Evans, E. O. Potma, M. Puoris’haag, D. Côté, C. P. Lin, and X. S. Xie, “Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy,” Proc. Natl. Acad. Sci. U.S.A. 102(46), 16807–16812 (2005).
[CrossRef] [PubMed]

Downes, A.

A. Downes, R. Mouras, and A. Elfick, “A versatile CARS microscope for biological imaging,” J. Raman Spectrosc. 40(7), 757–762 (2009).
[CrossRef]

Elfick, A.

A. Downes, R. Mouras, and A. Elfick, “A versatile CARS microscope for biological imaging,” J. Raman Spectrosc. 40(7), 757–762 (2009).
[CrossRef]

Evans, C. L.

C. L. Evans and X. S. Xie, “Coherent Anti-Stokes Raman Scattering Microscopy: Chemical Imaging for Biology and Medicine,” Ann. Rev. Anal. Chem. 1(1), 883–909 (2008).
[CrossRef]

C. L. Evans, X. Xu, S. Kesari, X. S. Xie, S. T. Wong, and G. S. Young, “Chemically-selective imaging of brain structures with CARS microscopy,” Opt. Express 15(19), 12076–12087 (2007).
[CrossRef] [PubMed]

F. Ganikhanov, C. L. Evans, B. G. Saar, and X. S. Xie, “High-sensitivity vibrational imaging with frequency modulation coherent anti-Stokes Raman scattering (FM CARS) microscopy,” Opt. Lett. 31(12), 1872–1874 (2006).
[CrossRef] [PubMed]

F. Légaré, C. L. Evans, F. Ganikhanov, and X. S. Xie, “Towards CARS Endoscopy,” Opt. Express 14(10), 4427–4432 (2006).
[CrossRef] [PubMed]

C. L. Evans, E. O. Potma, M. Puoris’haag, D. Côté, C. P. Lin, and X. S. Xie, “Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy,” Proc. Natl. Acad. Sci. U.S.A. 102(46), 16807–16812 (2005).
[CrossRef] [PubMed]

Flusberg, B. A.

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, “Fiber-optic fluorescence imaging,” Nat. Methods 2(12), 941–950 (2005).
[CrossRef] [PubMed]

Fried, N. M.

U. Sharma, N. M. Fried, and J. U. Kang, “All-fiber common-path optical coherence tomography: sensitivity optimization and system analysis,” IEEE J. Sel. Top. Quantum Electron. 11(4), 799–805 (2005).
[CrossRef]

Fujimoto, J. G.

G. J. Tearney, M. E. Brezinski, B. E. Bouma, S. A. Boppart, C. Pitris, J. F. Southern, and J. G. Fujimoto, “In vivo endoscopic optical biopsy with optical coherence tomography,” Science 276(5321), 2037–2039 (1997).
[CrossRef] [PubMed]

Ganikhanov, F.

Genet, M.

E. Laemmel, M. Genet, G. Le Goualher, A. Perchant, J. F. Le Gargasson, and E. Vicaut, “Fibered confocal fluorescence microscopy (Cell-viZio) facilitates extended imaging in the field of microcirculation. A comparison with intravital microscopy,” J. Vasc. Res. 41(5), 400–411 (2004).
[CrossRef] [PubMed]

Heflin, J. R.

Horiguchi, T.

Huff, T. B.

Inoue, K.

K. Inoue, “Four-wave mixing in an optical fiber in the zero-dispersion wavelength region,” J. Lightwave Technol. 10(11), 1553–1561 (1992).
[CrossRef]

Jung, J. C.

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, “Fiber-optic fluorescence imaging,” Nat. Methods 2(12), 941–950 (2005).
[CrossRef] [PubMed]

Kang, J. U.

U. Sharma, N. M. Fried, and J. U. Kang, “All-fiber common-path optical coherence tomography: sensitivity optimization and system analysis,” IEEE J. Sel. Top. Quantum Electron. 11(4), 799–805 (2005).
[CrossRef]

Kesari, S.

Kurokawa, K.

Laemmel, E.

E. Laemmel, M. Genet, G. Le Goualher, A. Perchant, J. F. Le Gargasson, and E. Vicaut, “Fibered confocal fluorescence microscopy (Cell-viZio) facilitates extended imaging in the field of microcirculation. A comparison with intravital microscopy,” J. Vasc. Res. 41(5), 400–411 (2004).
[CrossRef] [PubMed]

Le Gargasson, J. F.

E. Laemmel, M. Genet, G. Le Goualher, A. Perchant, J. F. Le Gargasson, and E. Vicaut, “Fibered confocal fluorescence microscopy (Cell-viZio) facilitates extended imaging in the field of microcirculation. A comparison with intravital microscopy,” J. Vasc. Res. 41(5), 400–411 (2004).
[CrossRef] [PubMed]

Le Goualher, G.

E. Laemmel, M. Genet, G. Le Goualher, A. Perchant, J. F. Le Gargasson, and E. Vicaut, “Fibered confocal fluorescence microscopy (Cell-viZio) facilitates extended imaging in the field of microcirculation. A comparison with intravital microscopy,” J. Vasc. Res. 41(5), 400–411 (2004).
[CrossRef] [PubMed]

Légaré, F.

Lin, C. P.

C. L. Evans, E. O. Potma, M. Puoris’haag, D. Côté, C. P. Lin, and X. S. Xie, “Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy,” Proc. Natl. Acad. Sci. U.S.A. 102(46), 16807–16812 (2005).
[CrossRef] [PubMed]

Liu, G.

Miyajima, Y.

Mouras, R.

A. Downes, R. Mouras, and A. Elfick, “A versatile CARS microscope for biological imaging,” J. Raman Spectrosc. 40(7), 757–762 (2009).
[CrossRef]

Müller, M.

M. Müller and A. Zumbusch, “Coherent anti-Stokes Raman scattering microscopy,” ChemPhysChem 8(15), 2156–2170 (2007).
[CrossRef] [PubMed]

M. Müller and J. M. Schins, “Imaging the Thermodynamic State of Lipid Membranes with Multiplex CARS Microscopy,” J. Phys. Chem. B 106(14), 3715–3723 (2002).
[CrossRef]

Nakajima, K.

Nan, X.

X. Nan, J. X. Cheng, and X. S. Xie, “Vibrational imaging of lipid droplets in live fibroblast cells with coherent anti-Stokes Raman scattering microscopy,” J. Lipid Res. 44(11), 2202–2208 (2003).
[CrossRef] [PubMed]

Ohashi, M.

Perchant, A.

E. Laemmel, M. Genet, G. Le Goualher, A. Perchant, J. F. Le Gargasson, and E. Vicaut, “Fibered confocal fluorescence microscopy (Cell-viZio) facilitates extended imaging in the field of microcirculation. A comparison with intravital microscopy,” J. Vasc. Res. 41(5), 400–411 (2004).
[CrossRef] [PubMed]

Pitris, C.

G. J. Tearney, M. E. Brezinski, B. E. Bouma, S. A. Boppart, C. Pitris, J. F. Southern, and J. G. Fujimoto, “In vivo endoscopic optical biopsy with optical coherence tomography,” Science 276(5321), 2037–2039 (1997).
[CrossRef] [PubMed]

Piyawattanametha, W.

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, “Fiber-optic fluorescence imaging,” Nat. Methods 2(12), 941–950 (2005).
[CrossRef] [PubMed]

Potma, E. O.

M. Balu, G. Liu, Z. Chen, B. J. Tromberg, and E. O. Potma, “Fiber delivered probe for efficient CARS imaging of tissues,” Opt. Express 18(3), 2380–2388 (2010).
[CrossRef] [PubMed]

C. L. Evans, E. O. Potma, M. Puoris’haag, D. Côté, C. P. Lin, and X. S. Xie, “Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy,” Proc. Natl. Acad. Sci. U.S.A. 102(46), 16807–16812 (2005).
[CrossRef] [PubMed]

Puoris’haag, M.

C. L. Evans, E. O. Potma, M. Puoris’haag, D. Côté, C. P. Lin, and X. S. Xie, “Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy,” Proc. Natl. Acad. Sci. U.S.A. 102(46), 16807–16812 (2005).
[CrossRef] [PubMed]

Ramachandran, S.

Saar, B. G.

Schins, J. M.

M. Müller and J. M. Schins, “Imaging the Thermodynamic State of Lipid Membranes with Multiplex CARS Microscopy,” J. Phys. Chem. B 106(14), 3715–3723 (2002).
[CrossRef]

Schnitzer, M. J.

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, “Fiber-optic fluorescence imaging,” Nat. Methods 2(12), 941–950 (2005).
[CrossRef] [PubMed]

Sharma, U.

U. Sharma, N. M. Fried, and J. U. Kang, “All-fiber common-path optical coherence tomography: sensitivity optimization and system analysis,” IEEE J. Sel. Top. Quantum Electron. 11(4), 799–805 (2005).
[CrossRef]

Shiraki, K.

Southern, J. F.

G. J. Tearney, M. E. Brezinski, B. E. Bouma, S. A. Boppart, C. Pitris, J. F. Southern, and J. G. Fujimoto, “In vivo endoscopic optical biopsy with optical coherence tomography,” Science 276(5321), 2037–2039 (1997).
[CrossRef] [PubMed]

Stolen, R.

Stolen, R. H.

R. H. Stolen, “Phase-matched-stimulated four-photon mixing in silica-fiber waveguides,” IEEE J. Quantum Electron. 11(3), 100–103 (1975).
[CrossRef]

Tearney, G. J.

G. J. Tearney, M. E. Brezinski, B. E. Bouma, S. A. Boppart, C. Pitris, J. F. Southern, and J. G. Fujimoto, “In vivo endoscopic optical biopsy with optical coherence tomography,” Science 276(5321), 2037–2039 (1997).
[CrossRef] [PubMed]

Tromberg, B. J.

Vicaut, E.

E. Laemmel, M. Genet, G. Le Goualher, A. Perchant, J. F. Le Gargasson, and E. Vicaut, “Fibered confocal fluorescence microscopy (Cell-viZio) facilitates extended imaging in the field of microcirculation. A comparison with intravital microscopy,” J. Vasc. Res. 41(5), 400–411 (2004).
[CrossRef] [PubMed]

Volkmer, A.

J.-X. Cheng, A. Volkmer, L. D. Book, and X. S. Xie, “Multiplex coherent anti-Stokes Raman scattering microspectroscopy and study of lipid vesicles,” J. Phys. Chem. B 106(34), 8493–8498 (2002).
[CrossRef]

Wang, H.

Wang, Z.

Wong, S. T.

Xie, X. S.

C. L. Evans and X. S. Xie, “Coherent Anti-Stokes Raman Scattering Microscopy: Chemical Imaging for Biology and Medicine,” Ann. Rev. Anal. Chem. 1(1), 883–909 (2008).
[CrossRef]

C. L. Evans, X. Xu, S. Kesari, X. S. Xie, S. T. Wong, and G. S. Young, “Chemically-selective imaging of brain structures with CARS microscopy,” Opt. Express 15(19), 12076–12087 (2007).
[CrossRef] [PubMed]

F. Ganikhanov, C. L. Evans, B. G. Saar, and X. S. Xie, “High-sensitivity vibrational imaging with frequency modulation coherent anti-Stokes Raman scattering (FM CARS) microscopy,” Opt. Lett. 31(12), 1872–1874 (2006).
[CrossRef] [PubMed]

F. Légaré, C. L. Evans, F. Ganikhanov, and X. S. Xie, “Towards CARS Endoscopy,” Opt. Express 14(10), 4427–4432 (2006).
[CrossRef] [PubMed]

C. L. Evans, E. O. Potma, M. Puoris’haag, D. Côté, C. P. Lin, and X. S. Xie, “Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy,” Proc. Natl. Acad. Sci. U.S.A. 102(46), 16807–16812 (2005).
[CrossRef] [PubMed]

J. X. Cheng and X. S. Xie, “Coherent anti-Stokes Raman scattering microscopy: instrumentation, theory, and applications,” J. Phys. Chem. B 108(3), 827–840 (2004).
[CrossRef]

X. Nan, J. X. Cheng, and X. S. Xie, “Vibrational imaging of lipid droplets in live fibroblast cells with coherent anti-Stokes Raman scattering microscopy,” J. Lipid Res. 44(11), 2202–2208 (2003).
[CrossRef] [PubMed]

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Figures (6)

Fig. 1
Fig. 1

Schematic of our CARS microscopy system with (a) pump and Stokes beams coupling into a fiber by a microscopy objective and collimated by a microscopy objective; (b) Configurations of the CARS microscope.

Fig. 2
Fig. 2

Simulated (a) dispersion length LD as a function of wavelength (black curve, tp = 5ps) and pulse width (red curve, λ = 817nm), respectively; (b) the shortest and longest Lw as a function of Raman shift with respective to Stokes wave (1064nm); (c) nonlinear length LNL (black curves for 817nm, green curves for 1064nm) and average threshold power P (red curve for 817nm, blue curve for 1064nm) as a function of the mode diameter.

Fig. 3
Fig. 3

(a) Normalized measured autocorrelation function curves of the pump (817nm, 50mW) and the Stokes (1064nm, 50mW) waves at two different conditions: 1. direct output from OPO or laser, and 2. After passing through 2-meter SMF28; (b) Normalized measured cross-correlation intensity curves before and after the pump (817nm) and Stokes (1064nm) waves passed though a 2-meter SMF28.

Fig. 4
Fig. 4

(a) Normalized measured pump (817nm) wave spectra as a function of power in SMF28; (b) Normalized measured Stokes (1064nm) wave spectra as a function of power in SMF28; (c) Normalized measured FWM (663nm) wave spectrum output from SMF28.

Fig. 5
Fig. 5

(a) Brightfield image of the well-cleaved proximal end of a 1-meter long SMF28; CARS images from proximal end of the 1-meter SMF28 at (b) 817nm + 1064nm and well-cleaved distal end suspending in air; (c) 817nm only and well-cleaved distal end suspending in air; (d) 817nm + 1064nm and well-cleaved distal end immersing in water; (e) 817nm + 1064nm and well-cleaved distal end immersing in oil; (f) 817nm + 1064nm and bad-cleaved/bad-cut distal end suspending in air.

Fig. 6
Fig. 6

CARS images were captured by CARS system with delivery of ps lasers through SMF28. Forward (a) and Epi (b) CARS images of 10μm polystyrene beads spin-coated on a glass slide, scale bar is 5μm. Epi CARS images of (c) mouse kidney and (d) mouse ear, scale bar is 10μm.

Equations (4)

Equations on this page are rendered with MathJax. Learn more.

L D = ( 2 π c λ 2 ) ( t P 2 D ) ,
L W = t P v g 1 ( λ 1 ) v g 1 ( λ 2 ) ,
L N L = π D e f f 2 λ f P t P 4 n 2 P a v e ,
P 2 π = π λ f P t P D e f f 2 4 n 2 L ,

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