Abstract

Fluorescence Correlation Spectroscopy (FCS) in cells often suffers from artifacts caused by bright aggregates or vesicles, depletion of fluorophores or bleaching of a fluorescent background. The common practice of manually discarding distorted curves is time consuming and subjective. Here we demonstrate the feasibility of automated FCS data analysis with efficient rejection of corrupted parts of the signal. As test systems we use a solution of fluorescent molecules, contaminated with bright fluorescent beads, as well as cells expressing a fluorescent protein (ICA512-EGFP), which partitions into bright secretory granules. This approach improves the accuracy of FCS measurements in biological samples, extends its applicability to especially challenging systems and greatly simplifies and accelerates the data analysis.

© 2010 Optical Society of America

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    [CrossRef]
  5. T. Dertinger, V. Pacheco, I. von der Hocht, R. Hartmann, I. Gregor, and J. Enderlein, "Two-Focus Fluorescence Correlation Spectroscopy: A New Tool for Accurate and Absolute Diffusion Measurements," ChemPhysChem 8(3), 433-443 (2007).
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  6. S. Kim, K. Heinze, and P. Schwille, "Fluorescence correlation spectroscopy in living cells," Nat. Methods 4(11), 963-974 (2007).
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  7. K. Bacia, S. Kim, and P. Schwille, "Fluorescence cross-correlation spectroscopy in living cells," Nat. Methods 3(2), 83-89 (2006).
    [CrossRef]
  8. J. Ries and P. Schwille, "New Concepts for Fluorescence Correlation Spectroscopy on Membranes," Phys. Chem. Chem. Phys. 10(24), 3487-3497 (2008).
    [CrossRef]
  9. S. R. Yu,M. Burkhardt, M. Nowak, J. Ries, Z. Petr’asek, S. Scholpp, P. Schwille, and M. Brand, "Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing molecules," Nature 461(7263), 533-536 (2009).
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  12. M. Asfari, D. Janjic, P. Meda, G. Li, P. A. Halban, and C. B. Wollheim, "Establishment of 2-mercaptoethanoldependent differentiated insulin-secreting cell lines," Endocrinology 130(1), 167-178 (1992).
    [CrossRef]
  13. M. Trajkovski, H. Mziaut, A. Altkruger, J. Ouwendijk, K. P. Knoch, S. Muller, and M. Solimena, "Nuclear translocation of an ICA512 cytosolic fragment couples granule exocytosis and insulin expression in beta-cells," J. Cell. Biol. 167(6), 1063-1074 (2004).
    [CrossRef]
  14. C. C. Guet, L. Bruneaux, T. L. Min, D. Siegal-Gaskins, I. Figueroa, T. Emonet, and P. Cluzel, "Minimally invasive determination of mRNA concentration in single living bacteria," Nucleic Acids Res. 36(12), e73 (2008).
    [CrossRef]
  15. G. Meacci, J. Ries, E. Fischer-Friedrich, N. Kahya, P. Schwille, and K. Kruse, "Mobility of Min-proteins in Escherichia coli measured by fluorescence correlation spectroscopy," Phys. Biol. 3(4), 255-263 (2006).
    [CrossRef]

2009 (2)

S. R. Yu,M. Burkhardt, M. Nowak, J. Ries, Z. Petr’asek, S. Scholpp, P. Schwille, and M. Brand, "Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing molecules," Nature 461(7263), 533-536 (2009).
[CrossRef]

A. Tcherniak, C. Reznik, S. Link, and C. F. Landes, "Fluorescence correlation spectroscopy: criteria for analysis in complex systems," Anal. Chem. 81(2), 746-754 (2009).
[CrossRef]

2008 (2)

J. Ries and P. Schwille, "New Concepts for Fluorescence Correlation Spectroscopy on Membranes," Phys. Chem. Chem. Phys. 10(24), 3487-3497 (2008).
[CrossRef]

C. C. Guet, L. Bruneaux, T. L. Min, D. Siegal-Gaskins, I. Figueroa, T. Emonet, and P. Cluzel, "Minimally invasive determination of mRNA concentration in single living bacteria," Nucleic Acids Res. 36(12), e73 (2008).
[CrossRef]

2007 (2)

T. Dertinger, V. Pacheco, I. von der Hocht, R. Hartmann, I. Gregor, and J. Enderlein, "Two-Focus Fluorescence Correlation Spectroscopy: A New Tool for Accurate and Absolute Diffusion Measurements," ChemPhysChem 8(3), 433-443 (2007).
[CrossRef]

S. Kim, K. Heinze, and P. Schwille, "Fluorescence correlation spectroscopy in living cells," Nat. Methods 4(11), 963-974 (2007).
[CrossRef]

2006 (2)

K. Bacia, S. Kim, and P. Schwille, "Fluorescence cross-correlation spectroscopy in living cells," Nat. Methods 3(2), 83-89 (2006).
[CrossRef]

G. Meacci, J. Ries, E. Fischer-Friedrich, N. Kahya, P. Schwille, and K. Kruse, "Mobility of Min-proteins in Escherichia coli measured by fluorescence correlation spectroscopy," Phys. Biol. 3(4), 255-263 (2006).
[CrossRef]

2004 (1)

M. Trajkovski, H. Mziaut, A. Altkruger, J. Ouwendijk, K. P. Knoch, S. Muller, and M. Solimena, "Nuclear translocation of an ICA512 cytosolic fragment couples granule exocytosis and insulin expression in beta-cells," J. Cell. Biol. 167(6), 1063-1074 (2004).
[CrossRef]

2003 (1)

K. Bacia and P. Schwille, "A dynamic view of cellular processes by in vivo fluorescence auto-and crosscorrelation spectroscopy," Methods 29(1), 74-85 (2003).
[CrossRef]

2001 (1)

1992 (1)

M. Asfari, D. Janjic, P. Meda, G. Li, P. A. Halban, and C. B. Wollheim, "Establishment of 2-mercaptoethanoldependent differentiated insulin-secreting cell lines," Endocrinology 130(1), 167-178 (1992).
[CrossRef]

1974 (1)

E. L. Elson and D. Magde, "Fluorescence correlation spectroscopy. I. Conceptual basis and theory," Biopolymers 13 (1), 1-27 (1974).
[CrossRef]

Altkruger, A.

M. Trajkovski, H. Mziaut, A. Altkruger, J. Ouwendijk, K. P. Knoch, S. Muller, and M. Solimena, "Nuclear translocation of an ICA512 cytosolic fragment couples granule exocytosis and insulin expression in beta-cells," J. Cell. Biol. 167(6), 1063-1074 (2004).
[CrossRef]

Asfari, M.

M. Asfari, D. Janjic, P. Meda, G. Li, P. A. Halban, and C. B. Wollheim, "Establishment of 2-mercaptoethanoldependent differentiated insulin-secreting cell lines," Endocrinology 130(1), 167-178 (1992).
[CrossRef]

Bacia, K.

K. Bacia, S. Kim, and P. Schwille, "Fluorescence cross-correlation spectroscopy in living cells," Nat. Methods 3(2), 83-89 (2006).
[CrossRef]

K. Bacia and P. Schwille, "A dynamic view of cellular processes by in vivo fluorescence auto-and crosscorrelation spectroscopy," Methods 29(1), 74-85 (2003).
[CrossRef]

Brand, M.

S. R. Yu,M. Burkhardt, M. Nowak, J. Ries, Z. Petr’asek, S. Scholpp, P. Schwille, and M. Brand, "Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing molecules," Nature 461(7263), 533-536 (2009).
[CrossRef]

Bruneaux, L.

C. C. Guet, L. Bruneaux, T. L. Min, D. Siegal-Gaskins, I. Figueroa, T. Emonet, and P. Cluzel, "Minimally invasive determination of mRNA concentration in single living bacteria," Nucleic Acids Res. 36(12), e73 (2008).
[CrossRef]

Burkhardt, M.

S. R. Yu,M. Burkhardt, M. Nowak, J. Ries, Z. Petr’asek, S. Scholpp, P. Schwille, and M. Brand, "Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing molecules," Nature 461(7263), 533-536 (2009).
[CrossRef]

Cluzel, P.

C. C. Guet, L. Bruneaux, T. L. Min, D. Siegal-Gaskins, I. Figueroa, T. Emonet, and P. Cluzel, "Minimally invasive determination of mRNA concentration in single living bacteria," Nucleic Acids Res. 36(12), e73 (2008).
[CrossRef]

Dertinger, T.

T. Dertinger, V. Pacheco, I. von der Hocht, R. Hartmann, I. Gregor, and J. Enderlein, "Two-Focus Fluorescence Correlation Spectroscopy: A New Tool for Accurate and Absolute Diffusion Measurements," ChemPhysChem 8(3), 433-443 (2007).
[CrossRef]

Elson, E. L.

E. L. Elson and D. Magde, "Fluorescence correlation spectroscopy. I. Conceptual basis and theory," Biopolymers 13 (1), 1-27 (1974).
[CrossRef]

Emonet, T.

C. C. Guet, L. Bruneaux, T. L. Min, D. Siegal-Gaskins, I. Figueroa, T. Emonet, and P. Cluzel, "Minimally invasive determination of mRNA concentration in single living bacteria," Nucleic Acids Res. 36(12), e73 (2008).
[CrossRef]

Enderlein, J.

T. Dertinger, V. Pacheco, I. von der Hocht, R. Hartmann, I. Gregor, and J. Enderlein, "Two-Focus Fluorescence Correlation Spectroscopy: A New Tool for Accurate and Absolute Diffusion Measurements," ChemPhysChem 8(3), 433-443 (2007).
[CrossRef]

Ferri, F.

Figueroa, I.

C. C. Guet, L. Bruneaux, T. L. Min, D. Siegal-Gaskins, I. Figueroa, T. Emonet, and P. Cluzel, "Minimally invasive determination of mRNA concentration in single living bacteria," Nucleic Acids Res. 36(12), e73 (2008).
[CrossRef]

Fischer-Friedrich, E.

G. Meacci, J. Ries, E. Fischer-Friedrich, N. Kahya, P. Schwille, and K. Kruse, "Mobility of Min-proteins in Escherichia coli measured by fluorescence correlation spectroscopy," Phys. Biol. 3(4), 255-263 (2006).
[CrossRef]

Gregor, I.

T. Dertinger, V. Pacheco, I. von der Hocht, R. Hartmann, I. Gregor, and J. Enderlein, "Two-Focus Fluorescence Correlation Spectroscopy: A New Tool for Accurate and Absolute Diffusion Measurements," ChemPhysChem 8(3), 433-443 (2007).
[CrossRef]

Guet, C. C.

C. C. Guet, L. Bruneaux, T. L. Min, D. Siegal-Gaskins, I. Figueroa, T. Emonet, and P. Cluzel, "Minimally invasive determination of mRNA concentration in single living bacteria," Nucleic Acids Res. 36(12), e73 (2008).
[CrossRef]

Halban, P. A.

M. Asfari, D. Janjic, P. Meda, G. Li, P. A. Halban, and C. B. Wollheim, "Establishment of 2-mercaptoethanoldependent differentiated insulin-secreting cell lines," Endocrinology 130(1), 167-178 (1992).
[CrossRef]

Hartmann, R.

T. Dertinger, V. Pacheco, I. von der Hocht, R. Hartmann, I. Gregor, and J. Enderlein, "Two-Focus Fluorescence Correlation Spectroscopy: A New Tool for Accurate and Absolute Diffusion Measurements," ChemPhysChem 8(3), 433-443 (2007).
[CrossRef]

Heinze, K.

S. Kim, K. Heinze, and P. Schwille, "Fluorescence correlation spectroscopy in living cells," Nat. Methods 4(11), 963-974 (2007).
[CrossRef]

Janjic, D.

M. Asfari, D. Janjic, P. Meda, G. Li, P. A. Halban, and C. B. Wollheim, "Establishment of 2-mercaptoethanoldependent differentiated insulin-secreting cell lines," Endocrinology 130(1), 167-178 (1992).
[CrossRef]

Kahya, N.

G. Meacci, J. Ries, E. Fischer-Friedrich, N. Kahya, P. Schwille, and K. Kruse, "Mobility of Min-proteins in Escherichia coli measured by fluorescence correlation spectroscopy," Phys. Biol. 3(4), 255-263 (2006).
[CrossRef]

Kim, S.

S. Kim, K. Heinze, and P. Schwille, "Fluorescence correlation spectroscopy in living cells," Nat. Methods 4(11), 963-974 (2007).
[CrossRef]

K. Bacia, S. Kim, and P. Schwille, "Fluorescence cross-correlation spectroscopy in living cells," Nat. Methods 3(2), 83-89 (2006).
[CrossRef]

Knoch, K. P.

M. Trajkovski, H. Mziaut, A. Altkruger, J. Ouwendijk, K. P. Knoch, S. Muller, and M. Solimena, "Nuclear translocation of an ICA512 cytosolic fragment couples granule exocytosis and insulin expression in beta-cells," J. Cell. Biol. 167(6), 1063-1074 (2004).
[CrossRef]

Kruse, K.

G. Meacci, J. Ries, E. Fischer-Friedrich, N. Kahya, P. Schwille, and K. Kruse, "Mobility of Min-proteins in Escherichia coli measured by fluorescence correlation spectroscopy," Phys. Biol. 3(4), 255-263 (2006).
[CrossRef]

Landes, C. F.

A. Tcherniak, C. Reznik, S. Link, and C. F. Landes, "Fluorescence correlation spectroscopy: criteria for analysis in complex systems," Anal. Chem. 81(2), 746-754 (2009).
[CrossRef]

Li, G.

M. Asfari, D. Janjic, P. Meda, G. Li, P. A. Halban, and C. B. Wollheim, "Establishment of 2-mercaptoethanoldependent differentiated insulin-secreting cell lines," Endocrinology 130(1), 167-178 (1992).
[CrossRef]

Link, S.

A. Tcherniak, C. Reznik, S. Link, and C. F. Landes, "Fluorescence correlation spectroscopy: criteria for analysis in complex systems," Anal. Chem. 81(2), 746-754 (2009).
[CrossRef]

Magatti, D.

Magde, D.

E. L. Elson and D. Magde, "Fluorescence correlation spectroscopy. I. Conceptual basis and theory," Biopolymers 13 (1), 1-27 (1974).
[CrossRef]

Meacci, G.

G. Meacci, J. Ries, E. Fischer-Friedrich, N. Kahya, P. Schwille, and K. Kruse, "Mobility of Min-proteins in Escherichia coli measured by fluorescence correlation spectroscopy," Phys. Biol. 3(4), 255-263 (2006).
[CrossRef]

Meda, P.

M. Asfari, D. Janjic, P. Meda, G. Li, P. A. Halban, and C. B. Wollheim, "Establishment of 2-mercaptoethanoldependent differentiated insulin-secreting cell lines," Endocrinology 130(1), 167-178 (1992).
[CrossRef]

Min, T. L.

C. C. Guet, L. Bruneaux, T. L. Min, D. Siegal-Gaskins, I. Figueroa, T. Emonet, and P. Cluzel, "Minimally invasive determination of mRNA concentration in single living bacteria," Nucleic Acids Res. 36(12), e73 (2008).
[CrossRef]

Muller, S.

M. Trajkovski, H. Mziaut, A. Altkruger, J. Ouwendijk, K. P. Knoch, S. Muller, and M. Solimena, "Nuclear translocation of an ICA512 cytosolic fragment couples granule exocytosis and insulin expression in beta-cells," J. Cell. Biol. 167(6), 1063-1074 (2004).
[CrossRef]

Mziaut, H.

M. Trajkovski, H. Mziaut, A. Altkruger, J. Ouwendijk, K. P. Knoch, S. Muller, and M. Solimena, "Nuclear translocation of an ICA512 cytosolic fragment couples granule exocytosis and insulin expression in beta-cells," J. Cell. Biol. 167(6), 1063-1074 (2004).
[CrossRef]

Nowak, M.

S. R. Yu,M. Burkhardt, M. Nowak, J. Ries, Z. Petr’asek, S. Scholpp, P. Schwille, and M. Brand, "Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing molecules," Nature 461(7263), 533-536 (2009).
[CrossRef]

Ouwendijk, J.

M. Trajkovski, H. Mziaut, A. Altkruger, J. Ouwendijk, K. P. Knoch, S. Muller, and M. Solimena, "Nuclear translocation of an ICA512 cytosolic fragment couples granule exocytosis and insulin expression in beta-cells," J. Cell. Biol. 167(6), 1063-1074 (2004).
[CrossRef]

Pacheco, V.

T. Dertinger, V. Pacheco, I. von der Hocht, R. Hartmann, I. Gregor, and J. Enderlein, "Two-Focus Fluorescence Correlation Spectroscopy: A New Tool for Accurate and Absolute Diffusion Measurements," ChemPhysChem 8(3), 433-443 (2007).
[CrossRef]

Petr’asek, Z.

S. R. Yu,M. Burkhardt, M. Nowak, J. Ries, Z. Petr’asek, S. Scholpp, P. Schwille, and M. Brand, "Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing molecules," Nature 461(7263), 533-536 (2009).
[CrossRef]

Reznik, C.

A. Tcherniak, C. Reznik, S. Link, and C. F. Landes, "Fluorescence correlation spectroscopy: criteria for analysis in complex systems," Anal. Chem. 81(2), 746-754 (2009).
[CrossRef]

Ries, J.

S. R. Yu,M. Burkhardt, M. Nowak, J. Ries, Z. Petr’asek, S. Scholpp, P. Schwille, and M. Brand, "Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing molecules," Nature 461(7263), 533-536 (2009).
[CrossRef]

J. Ries and P. Schwille, "New Concepts for Fluorescence Correlation Spectroscopy on Membranes," Phys. Chem. Chem. Phys. 10(24), 3487-3497 (2008).
[CrossRef]

G. Meacci, J. Ries, E. Fischer-Friedrich, N. Kahya, P. Schwille, and K. Kruse, "Mobility of Min-proteins in Escherichia coli measured by fluorescence correlation spectroscopy," Phys. Biol. 3(4), 255-263 (2006).
[CrossRef]

Scholpp, S.

S. R. Yu,M. Burkhardt, M. Nowak, J. Ries, Z. Petr’asek, S. Scholpp, P. Schwille, and M. Brand, "Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing molecules," Nature 461(7263), 533-536 (2009).
[CrossRef]

Schwille, P.

S. R. Yu,M. Burkhardt, M. Nowak, J. Ries, Z. Petr’asek, S. Scholpp, P. Schwille, and M. Brand, "Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing molecules," Nature 461(7263), 533-536 (2009).
[CrossRef]

J. Ries and P. Schwille, "New Concepts for Fluorescence Correlation Spectroscopy on Membranes," Phys. Chem. Chem. Phys. 10(24), 3487-3497 (2008).
[CrossRef]

S. Kim, K. Heinze, and P. Schwille, "Fluorescence correlation spectroscopy in living cells," Nat. Methods 4(11), 963-974 (2007).
[CrossRef]

K. Bacia, S. Kim, and P. Schwille, "Fluorescence cross-correlation spectroscopy in living cells," Nat. Methods 3(2), 83-89 (2006).
[CrossRef]

G. Meacci, J. Ries, E. Fischer-Friedrich, N. Kahya, P. Schwille, and K. Kruse, "Mobility of Min-proteins in Escherichia coli measured by fluorescence correlation spectroscopy," Phys. Biol. 3(4), 255-263 (2006).
[CrossRef]

K. Bacia and P. Schwille, "A dynamic view of cellular processes by in vivo fluorescence auto-and crosscorrelation spectroscopy," Methods 29(1), 74-85 (2003).
[CrossRef]

Siegal-Gaskins, D.

C. C. Guet, L. Bruneaux, T. L. Min, D. Siegal-Gaskins, I. Figueroa, T. Emonet, and P. Cluzel, "Minimally invasive determination of mRNA concentration in single living bacteria," Nucleic Acids Res. 36(12), e73 (2008).
[CrossRef]

Solimena, M.

M. Trajkovski, H. Mziaut, A. Altkruger, J. Ouwendijk, K. P. Knoch, S. Muller, and M. Solimena, "Nuclear translocation of an ICA512 cytosolic fragment couples granule exocytosis and insulin expression in beta-cells," J. Cell. Biol. 167(6), 1063-1074 (2004).
[CrossRef]

Tcherniak, A.

A. Tcherniak, C. Reznik, S. Link, and C. F. Landes, "Fluorescence correlation spectroscopy: criteria for analysis in complex systems," Anal. Chem. 81(2), 746-754 (2009).
[CrossRef]

Trajkovski, M.

M. Trajkovski, H. Mziaut, A. Altkruger, J. Ouwendijk, K. P. Knoch, S. Muller, and M. Solimena, "Nuclear translocation of an ICA512 cytosolic fragment couples granule exocytosis and insulin expression in beta-cells," J. Cell. Biol. 167(6), 1063-1074 (2004).
[CrossRef]

von der Hocht, I.

T. Dertinger, V. Pacheco, I. von der Hocht, R. Hartmann, I. Gregor, and J. Enderlein, "Two-Focus Fluorescence Correlation Spectroscopy: A New Tool for Accurate and Absolute Diffusion Measurements," ChemPhysChem 8(3), 433-443 (2007).
[CrossRef]

Wollheim, C. B.

M. Asfari, D. Janjic, P. Meda, G. Li, P. A. Halban, and C. B. Wollheim, "Establishment of 2-mercaptoethanoldependent differentiated insulin-secreting cell lines," Endocrinology 130(1), 167-178 (1992).
[CrossRef]

Yu, S. R.

S. R. Yu,M. Burkhardt, M. Nowak, J. Ries, Z. Petr’asek, S. Scholpp, P. Schwille, and M. Brand, "Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing molecules," Nature 461(7263), 533-536 (2009).
[CrossRef]

Anal. Chem. (1)

A. Tcherniak, C. Reznik, S. Link, and C. F. Landes, "Fluorescence correlation spectroscopy: criteria for analysis in complex systems," Anal. Chem. 81(2), 746-754 (2009).
[CrossRef]

Appl. Opt. (1)

Biopolymers (1)

E. L. Elson and D. Magde, "Fluorescence correlation spectroscopy. I. Conceptual basis and theory," Biopolymers 13 (1), 1-27 (1974).
[CrossRef]

ChemPhysChem (1)

T. Dertinger, V. Pacheco, I. von der Hocht, R. Hartmann, I. Gregor, and J. Enderlein, "Two-Focus Fluorescence Correlation Spectroscopy: A New Tool for Accurate and Absolute Diffusion Measurements," ChemPhysChem 8(3), 433-443 (2007).
[CrossRef]

Endocrinology (1)

M. Asfari, D. Janjic, P. Meda, G. Li, P. A. Halban, and C. B. Wollheim, "Establishment of 2-mercaptoethanoldependent differentiated insulin-secreting cell lines," Endocrinology 130(1), 167-178 (1992).
[CrossRef]

J. Cell. Biol. (1)

M. Trajkovski, H. Mziaut, A. Altkruger, J. Ouwendijk, K. P. Knoch, S. Muller, and M. Solimena, "Nuclear translocation of an ICA512 cytosolic fragment couples granule exocytosis and insulin expression in beta-cells," J. Cell. Biol. 167(6), 1063-1074 (2004).
[CrossRef]

Methods (1)

K. Bacia and P. Schwille, "A dynamic view of cellular processes by in vivo fluorescence auto-and crosscorrelation spectroscopy," Methods 29(1), 74-85 (2003).
[CrossRef]

Nat. Methods (2)

S. Kim, K. Heinze, and P. Schwille, "Fluorescence correlation spectroscopy in living cells," Nat. Methods 4(11), 963-974 (2007).
[CrossRef]

K. Bacia, S. Kim, and P. Schwille, "Fluorescence cross-correlation spectroscopy in living cells," Nat. Methods 3(2), 83-89 (2006).
[CrossRef]

Nature (1)

S. R. Yu,M. Burkhardt, M. Nowak, J. Ries, Z. Petr’asek, S. Scholpp, P. Schwille, and M. Brand, "Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing molecules," Nature 461(7263), 533-536 (2009).
[CrossRef]

Nucleic Acids Res. (1)

C. C. Guet, L. Bruneaux, T. L. Min, D. Siegal-Gaskins, I. Figueroa, T. Emonet, and P. Cluzel, "Minimally invasive determination of mRNA concentration in single living bacteria," Nucleic Acids Res. 36(12), e73 (2008).
[CrossRef]

Phys. Biol. (1)

G. Meacci, J. Ries, E. Fischer-Friedrich, N. Kahya, P. Schwille, and K. Kruse, "Mobility of Min-proteins in Escherichia coli measured by fluorescence correlation spectroscopy," Phys. Biol. 3(4), 255-263 (2006).
[CrossRef]

Phys. Chem. Chem. Phys. (1)

J. Ries and P. Schwille, "New Concepts for Fluorescence Correlation Spectroscopy on Membranes," Phys. Chem. Chem. Phys. 10(24), 3487-3497 (2008).
[CrossRef]

Other (2)

R. Rigler and E. Elson, Fluorescence Correlation Spectroscopy: Theory and Applications, (Springer, 2001).

E. P. Petrov and P. Schwille, State of the art and novel trends in fluorescence correlation spectroscopy, in: Standardization in Fluorometry: State of the Art and Future Challenges, (Springer, Berlin Heidelberg New York, 2007).

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