Abstract

We report the potential of Mueller decomposition images to discriminate the normal against the dysplastic (precancerous) states in cervical tissue. It is observed that in the epithelium region, depolarization power is sensitive to morphological changes during progression from normal to dysplastic state while retardance and diattenuation do not show any significant change. These morphological changes have been correlated with the microscopic images of the tissues. In contrast, it is the retardance which reveals the morphological changes around the stromal region. Additionally, we have evaluated the arithmetic mean of depolarization power and retardance from their respective decomposed images and have shown that this parameter has a potential to discriminate normal tissues against dysplastic ones.

© 2009 Optical Society of America

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2007 (2)

D. Arifler, I. Pavlova, A. Gillenwater, and R. R. Kortum, "Light Scattering from Collagen Fiber Networks: Micro-Optical Properties of Normal and Neoplastic Stroma," J. Biophys. 92, 3260 - 3274 (2007).
[CrossRef]

Y. Deng, S. Zeng, Q. Lu, D. Zhu, and Q. Luo, "Characterization of back scattering muller matrix patterns of highly scattering media with triple scattering assumption," Opt. Express 15, 9672 - 9680 (2007).
[CrossRef] [PubMed]

2006 (2)

2004 (2)

2002 (1)

G. L. Liu, Y. Li, and B. D. Cameron, "Polarization Based Optical imaging and processing techniques with application to the Cancer Diagnostics," Proc. SPIE 4617, 208 - 219 (2002).
[CrossRef]

2001 (1)

2000 (1)

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. van Dam, and M. S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35 - 36 (2000).
[CrossRef] [PubMed]

1999 (1)

1998 (1)

1997 (2)

1996 (1)

Arendt, J. T.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. van Dam, and M. S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35 - 36 (2000).
[CrossRef] [PubMed]

Arifler, D.

D. Arifler, I. Pavlova, A. Gillenwater, and R. R. Kortum, "Light Scattering from Collagen Fiber Networks: Micro-Optical Properties of Normal and Neoplastic Stroma," J. Biophys. 92, 3260 - 3274 (2007).
[CrossRef]

Backman, V.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. van Dam, and M. S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35 - 36 (2000).
[CrossRef] [PubMed]

Badizadegan, K.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. van Dam, and M. S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35 - 36 (2000).
[CrossRef] [PubMed]

Baravian, C.

Berezhnyy, I.

Bigio, I. J.

Buddhiwant, P.

Cameron, B. D.

Caton, F.

Chipman, R. A.

Cote, G. L

Cote, G. L.

Crawford, J. M.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. van Dam, and M. S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35 - 36 (2000).
[CrossRef] [PubMed]

Dasari, R. R.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. van Dam, and M. S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35 - 36 (2000).
[CrossRef] [PubMed]

Deng, Y.

Dillet, J.

Dogariu, A.

Eick, A. A.

Feld, M. S.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. van Dam, and M. S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35 - 36 (2000).
[CrossRef] [PubMed]

Fitzmaurice, M.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. van Dam, and M. S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35 - 36 (2000).
[CrossRef] [PubMed]

Freyer, J. P.

Ghosh, N.

Gillenwater, A.

D. Arifler, I. Pavlova, A. Gillenwater, and R. R. Kortum, "Light Scattering from Collagen Fiber Networks: Micro-Optical Properties of Normal and Neoplastic Stroma," J. Biophys. 92, 3260 - 3274 (2007).
[CrossRef]

Goudail, F.

Gupta, P. K.

Gurjar, R.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. van Dam, and M. S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35 - 36 (2000).
[CrossRef] [PubMed]

Hielscher, A. H.

Itzkan, I.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. van Dam, and M. S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35 - 36 (2000).
[CrossRef] [PubMed]

Kabani, S.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. van Dam, and M. S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35 - 36 (2000).
[CrossRef] [PubMed]

Kaplan, B.

Kattawar, G. W.

Kline, E.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. van Dam, and M. S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35 - 36 (2000).
[CrossRef] [PubMed]

Kortum, R. R.

D. Arifler, I. Pavlova, A. Gillenwater, and R. R. Kortum, "Light Scattering from Collagen Fiber Networks: Micro-Optical Properties of Normal and Neoplastic Stroma," J. Biophys. 92, 3260 - 3274 (2007).
[CrossRef]

Levin, H. S.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. van Dam, and M. S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35 - 36 (2000).
[CrossRef] [PubMed]

Li, Y.

G. L. Liu, Y. Li, and B. D. Cameron, "Polarization Based Optical imaging and processing techniques with application to the Cancer Diagnostics," Proc. SPIE 4617, 208 - 219 (2002).
[CrossRef]

Liu, G. L.

G. L. Liu, Y. Li, and B. D. Cameron, "Polarization Based Optical imaging and processing techniques with application to the Cancer Diagnostics," Proc. SPIE 4617, 208 - 219 (2002).
[CrossRef]

Lu, Q.

Lu, S.Y.

Luo, Q.

Manhas, S.

McGillican, T.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. van Dam, and M. S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35 - 36 (2000).
[CrossRef] [PubMed]

Mehrubeoglu, M.

Morio, J.

Mourant, J. R.

Muller, M. G.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. van Dam, and M. S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35 - 36 (2000).
[CrossRef] [PubMed]

Parker, A.

Pavlova, I.

D. Arifler, I. Pavlova, A. Gillenwater, and R. R. Kortum, "Light Scattering from Collagen Fiber Networks: Micro-Optical Properties of Normal and Neoplastic Stroma," J. Biophys. 92, 3260 - 3274 (2007).
[CrossRef]

Perelman, L. T.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. van Dam, and M. S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35 - 36 (2000).
[CrossRef] [PubMed]

Rakovic, M. J.

Rastegar, S.

Seiler, M.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. van Dam, and M. S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35 - 36 (2000).
[CrossRef] [PubMed]

Shapshay, S.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. van Dam, and M. S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35 - 36 (2000).
[CrossRef] [PubMed]

Shen, D.

Swami, M. K.

Uppal, A.

Valdez, T.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. van Dam, and M. S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35 - 36 (2000).
[CrossRef] [PubMed]

van Dam, J.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. van Dam, and M. S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35 - 36 (2000).
[CrossRef] [PubMed]

Wallace, M. B.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. van Dam, and M. S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35 - 36 (2000).
[CrossRef] [PubMed]

Wang, L. V.

Zeng, S.

Zhang, Q.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. van Dam, and M. S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35 - 36 (2000).
[CrossRef] [PubMed]

Zhu, D.

Zonios, G.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. van Dam, and M. S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35 - 36 (2000).
[CrossRef] [PubMed]

Appl. Opt. (4)

J. Biophys. (1)

D. Arifler, I. Pavlova, A. Gillenwater, and R. R. Kortum, "Light Scattering from Collagen Fiber Networks: Micro-Optical Properties of Normal and Neoplastic Stroma," J. Biophys. 92, 3260 - 3274 (2007).
[CrossRef]

J. Opt. Soc. Am. A (1)

Nature (1)

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. van Dam, and M. S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35 - 36 (2000).
[CrossRef] [PubMed]

Opt. Express (4)

Opt. Lett. (2)

Proc. SPIE (1)

G. L. Liu, Y. Li, and B. D. Cameron, "Polarization Based Optical imaging and processing techniques with application to the Cancer Diagnostics," Proc. SPIE 4617, 208 - 219 (2002).
[CrossRef]

Other (4)

M. Anastasiadou, A. De Martino, D. Clement, F. Liège, B. Laude-Boulesteix, N. Quang, J. Dreyfuss, B. Huynh, A. NazacL. Schwartz, and H. Cohen, "Polarimetric imaging for the diagnosis of cervical cancer," Phys. Stat. Sol. (C) 5,1423-1426 (2008).
[CrossRef]

S. Firdous and M. Ikram, "Characterization of turbid medium through diffusely backscattering polarized light with matrix calculus-II," in Proceedings of IEEE Conference on International Networking and Communication (INCC 2004) pp. 115 - 123.

Y. Deng, Q. Lu, Q. Luo, and S. Zeng, "Third order scattering model for the diffuse backscattering intensity patterns of polarized light from a turbid medium," Appl. Phys. Lett. 90, 153902 (1) -153902 (3) (2007).
[CrossRef]

R. S. Cotran, V. Kumar, and S. L. Robbins, Robbins Pathologic Basis of Disease (W. B. Saunders Company, 1989), Chap. 24.

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Figures (6)

Fig. 1.
Fig. 1.

(a) Mueller image M00 and (b) the microscopic image of a dysplastic cervical tissue for the entire illuminated region.

Fig. 2.
Fig. 2.

Images of (a) depolarization power (b) diattenuation (c) retardance of a dysplastic cervical tissue section for the entire illuminated region.

Fig. 3.
Fig. 3.

Typical depolarization power images of (a) normal and (b) dysplastic state in the epithelium region of the cervical tissue section.

Fig. 4.
Fig. 4.

Microscopic images of epithelial layer of (a) normal and (b) dysplastic state.

Fig. 5.
Fig. 5.

Typical retardance images of (a) normal and (b) dysplastic state in the stromal region of the cervical tissue section.

Fig. 6.
Fig. 6.

(a) Mean value of depolarization power for the epithelium region and (b) mean value of retardance for the stromal region.

Equations (21)

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M = ( m 00 m 01 m 02 m 03 m 10 m 11 m 12 m 13 m 20 m 21 m 22 m 23 m 30 m 31 m 32 m 33 )
M = M Δ M R M D
D = ( m 01 2 + m 02 2 + m 03 3 ) 1 / 2 m 00
D = 1 m 00 ( m 01 m 02 m 03 )
M D = ( 1 D T D m D )
m D = 1 D 2 I + ( 1 1 D 2 ) D ̂ D ̂ T
M = M M D 1
M Δ M R = ( 1 0 P Δ m Δ ) ( 1 0 0 m R )
= ( 1 0 P Δ m Δ m R ) ( 1 0 P Δ m | )
= M
P Δ = P m D 1 D 2
m = m Δ m R
P = 1 m 00 ( m 10 m 20 m 30 )
m Δ = ± [ m ( m ) T + ( λ 1 λ 2 + λ 2 λ 3 + λ 3 λ 1 ) 1 / 2 I ] 1
× [ { ( λ 1 ) 1 / 2 + ( λ 2 ) 1 / 2 + ( λ 3 ) 1 / 2 } m ( m ) T + ( λ 1 λ 2 λ 3 ) 1 / 2 I ]
Δ = 1 tr ( M Δ ) 1 3
M R = M Δ 1 M
R = cos 1 [ tr ( M R ) 2 1 ]
D = 1 m 00 [ m 01 2 + m 02 2 + m 03 2 ] 1 / 2
Δ = 1 tr ( M Δ ) 1 3
R = cos 1 [ tr ( M R ) 2 1 ]

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