Abstract

Spectral properties of a recently developed voltage-sensitive dye, di-4-ANEPPDHQ, were characterized as the dye was dissolved in the solvent dimethyl sulfoxide as the stock solution, in Hank's buffered salt solution as the staining solution, and bound to the plasma membrane of primary rat hippocampal neurons and immortalized mouse hypothalamic neurons (GT1-7) in vitro. Their dependence on the local chemical and electrical environment of dye molecules was determined. The excitation and emission peaks are 479nm and 570 nm for the stained primary neurons, and 476nm and 585 nm for the stained immortalized neurons. The excitation and emission bands of the stained GT1-7 neurons, defined as 50% peak intensity, are 429-516nm and 544-648nm, respectively.

© 2009 Optical Society of America

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    [CrossRef] [PubMed]

2008 (2)

J. A. N. Fisher, J. R. Barchi, C. G. Welle, G.-H. Kim, P. Kosterin, A. L. Obaid, A. G. Yodh, D. Contreras and B. M. Salzberg, "Two-photon excitation of potentiometric probes enables optical recording of action potentials from mammalian nerve terminals in situ," J. Neurophysiol. 99, 1545-1553 (2008).
[CrossRef] [PubMed]

Y. Roche, P. Gerbeau-Pissot, B. Buhot, D. Thomas, L. Bonneau, J. Gresti, S Mongrand, J.-M. Perrier-Cornet, and F. Simon-Plas, "Depletion of phytosterols from the plant plasma membrane provides evidence for disruption of lipid rafts," The FASEB Journal 22, 1-12 (2008).
[CrossRef]

2007 (1)

D. M. Owen, M. A.A. Neil, P. M.W. French and A. I. Magee, "Optical techniques for imaging membrane lipid microdomains in living cells," Semin. Cell Biol. 18, 591-598 (2007).
[CrossRef]

2006 (3)

L. Jin, A. C. Millard, J. P. Wuskell, X. Dong, D. Wu, H. A. Clark, and L. M. Loew, "Characterization and application of a new optical probe for membrane lipid domains," Biophys. J. 90, 2563-2575 (2006).
[CrossRef] [PubMed]

B. Leuner, E. Gould, and T. J. Shors, "Is there a link between adult neurogenesis and learning?" Hippocampus 16, 216-224 (2006).
[CrossRef] [PubMed]

D. M. Owen, P. M. P. Lanigan, C. Dunsby, I. Munro, D. Grant, M. A. A. Neil, P. M. W. French, and A. I. Magee, "Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells." Biophys. J. 90, L80 - L82 (2006).
[CrossRef] [PubMed]

2005 (1)

A. L. Obaid, M. E. Nelson, J. Lindstrom and B. M. Salzberg, "Optical studies of nicotinic acetylcholine receptor subtypes in the guinea-pig," J. Experimental Bio. 208, 2981-3001 (2005).
[CrossRef]

2004 (1)

A. L. Obaid, L. M. Loew, J. P. Wuskell, B. M. Salzberg, "Novel naphthylstyryl-pyridinium potentiometric dyes offer advantages for neural network analysis," J. Neurosci. Methods 134, 179-190 (2004).
[CrossRef] [PubMed]

2003 (1)

M. Djurisic, M. Zochowski, M. Wachowiak, C. X. Falk, L. B. Cohen, and D. Zecevic, "Optical monitoring of neural activity using voltage-sensitive dyes," Methods Enzymol. 361, 423-451 (2003).
[CrossRef] [PubMed]

1996 (1)

L. M. Loew, "Potentiometric dyes: imaging electrical activity of cell membranes," Pure Appl. Chem. 68, 1405-1409 (1996).
[CrossRef]

1993 (1)

G. J. Brewer, J. R. Torricelli, E. K. Evege, and P. J. Price, "Optimized survival of hippocampal neurons in B27 -supplemented neurobasal, a new serum-free medium combination," J. Neurosci. Res. 35, 567-576 (1993).
[CrossRef] [PubMed]

1992 (1)

L. M. Loew, L. B. Cohen, J. Dix, E. N. Fluhler, V. Montana, G. Salama, and J. Y. Wu, "A naphthyl analog of the aminostyryl pyridinium class of potentiometric membrane dyes shows consistent sensitivity in a variety of tissue, cell, and model membrane preparations," J. Membrane Bio. 130, 1-10 (1992).

1991 (2)

P. Fromherz, K. H. Dambacher, H. Ephardt, A. Lambacher, C. O. Müller, R. Neigl, H. Schaden, O. Schenk, and T. Vetter, "Fluorescent dyes as probes of voltage transients in neuron membranes," Berichte der Bunsengesellschaft fuer Physikalische Chemie 95, 1333-1345 (1991).

Z. Liposits, I. Merchenthaler, W. C. Wetsel, J. J. Reid, P. L. Mellon, R. I. Weiner, and A. Negro-Vilar, "Morphological characterization of immortalized hypothalamic neurons synthesizing luteinizing hormone-releasing hormone," Endocrinology 129, 1575-1583 (1991).
[CrossRef] [PubMed]

1990 (1)

P. L. Mellon, J. J. Windle, P. C. Goldsmith, C. A. Padula, J. L. Roberts, and R. I. Weiner, "Immortalization of hypothalamic GnRH neurons by genetically targeted tumorigenesis," Neuron 5, 1-10 (1990).
[CrossRef] [PubMed]

Barchi, J. R.

J. A. N. Fisher, J. R. Barchi, C. G. Welle, G.-H. Kim, P. Kosterin, A. L. Obaid, A. G. Yodh, D. Contreras and B. M. Salzberg, "Two-photon excitation of potentiometric probes enables optical recording of action potentials from mammalian nerve terminals in situ," J. Neurophysiol. 99, 1545-1553 (2008).
[CrossRef] [PubMed]

Bonneau, L.

Y. Roche, P. Gerbeau-Pissot, B. Buhot, D. Thomas, L. Bonneau, J. Gresti, S Mongrand, J.-M. Perrier-Cornet, and F. Simon-Plas, "Depletion of phytosterols from the plant plasma membrane provides evidence for disruption of lipid rafts," The FASEB Journal 22, 1-12 (2008).
[CrossRef]

Brewer, G. J.

G. J. Brewer, J. R. Torricelli, E. K. Evege, and P. J. Price, "Optimized survival of hippocampal neurons in B27 -supplemented neurobasal, a new serum-free medium combination," J. Neurosci. Res. 35, 567-576 (1993).
[CrossRef] [PubMed]

Buhot, B.

Y. Roche, P. Gerbeau-Pissot, B. Buhot, D. Thomas, L. Bonneau, J. Gresti, S Mongrand, J.-M. Perrier-Cornet, and F. Simon-Plas, "Depletion of phytosterols from the plant plasma membrane provides evidence for disruption of lipid rafts," The FASEB Journal 22, 1-12 (2008).
[CrossRef]

Clark, H. A.

L. Jin, A. C. Millard, J. P. Wuskell, X. Dong, D. Wu, H. A. Clark, and L. M. Loew, "Characterization and application of a new optical probe for membrane lipid domains," Biophys. J. 90, 2563-2575 (2006).
[CrossRef] [PubMed]

Cohen, L. B.

M. Djurisic, M. Zochowski, M. Wachowiak, C. X. Falk, L. B. Cohen, and D. Zecevic, "Optical monitoring of neural activity using voltage-sensitive dyes," Methods Enzymol. 361, 423-451 (2003).
[CrossRef] [PubMed]

L. M. Loew, L. B. Cohen, J. Dix, E. N. Fluhler, V. Montana, G. Salama, and J. Y. Wu, "A naphthyl analog of the aminostyryl pyridinium class of potentiometric membrane dyes shows consistent sensitivity in a variety of tissue, cell, and model membrane preparations," J. Membrane Bio. 130, 1-10 (1992).

Contreras, D.

J. A. N. Fisher, J. R. Barchi, C. G. Welle, G.-H. Kim, P. Kosterin, A. L. Obaid, A. G. Yodh, D. Contreras and B. M. Salzberg, "Two-photon excitation of potentiometric probes enables optical recording of action potentials from mammalian nerve terminals in situ," J. Neurophysiol. 99, 1545-1553 (2008).
[CrossRef] [PubMed]

Dambacher, K. H.

P. Fromherz, K. H. Dambacher, H. Ephardt, A. Lambacher, C. O. Müller, R. Neigl, H. Schaden, O. Schenk, and T. Vetter, "Fluorescent dyes as probes of voltage transients in neuron membranes," Berichte der Bunsengesellschaft fuer Physikalische Chemie 95, 1333-1345 (1991).

Dix, J.

L. M. Loew, L. B. Cohen, J. Dix, E. N. Fluhler, V. Montana, G. Salama, and J. Y. Wu, "A naphthyl analog of the aminostyryl pyridinium class of potentiometric membrane dyes shows consistent sensitivity in a variety of tissue, cell, and model membrane preparations," J. Membrane Bio. 130, 1-10 (1992).

Djurisic, M.

M. Djurisic, M. Zochowski, M. Wachowiak, C. X. Falk, L. B. Cohen, and D. Zecevic, "Optical monitoring of neural activity using voltage-sensitive dyes," Methods Enzymol. 361, 423-451 (2003).
[CrossRef] [PubMed]

Dong, X.

L. Jin, A. C. Millard, J. P. Wuskell, X. Dong, D. Wu, H. A. Clark, and L. M. Loew, "Characterization and application of a new optical probe for membrane lipid domains," Biophys. J. 90, 2563-2575 (2006).
[CrossRef] [PubMed]

Dunsby, C.

D. M. Owen, P. M. P. Lanigan, C. Dunsby, I. Munro, D. Grant, M. A. A. Neil, P. M. W. French, and A. I. Magee, "Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells." Biophys. J. 90, L80 - L82 (2006).
[CrossRef] [PubMed]

Ephardt, H.

P. Fromherz, K. H. Dambacher, H. Ephardt, A. Lambacher, C. O. Müller, R. Neigl, H. Schaden, O. Schenk, and T. Vetter, "Fluorescent dyes as probes of voltage transients in neuron membranes," Berichte der Bunsengesellschaft fuer Physikalische Chemie 95, 1333-1345 (1991).

Evege, E. K.

G. J. Brewer, J. R. Torricelli, E. K. Evege, and P. J. Price, "Optimized survival of hippocampal neurons in B27 -supplemented neurobasal, a new serum-free medium combination," J. Neurosci. Res. 35, 567-576 (1993).
[CrossRef] [PubMed]

Falk, C. X.

M. Djurisic, M. Zochowski, M. Wachowiak, C. X. Falk, L. B. Cohen, and D. Zecevic, "Optical monitoring of neural activity using voltage-sensitive dyes," Methods Enzymol. 361, 423-451 (2003).
[CrossRef] [PubMed]

Fisher, J. A. N.

J. A. N. Fisher, J. R. Barchi, C. G. Welle, G.-H. Kim, P. Kosterin, A. L. Obaid, A. G. Yodh, D. Contreras and B. M. Salzberg, "Two-photon excitation of potentiometric probes enables optical recording of action potentials from mammalian nerve terminals in situ," J. Neurophysiol. 99, 1545-1553 (2008).
[CrossRef] [PubMed]

Fluhler, E. N.

L. M. Loew, L. B. Cohen, J. Dix, E. N. Fluhler, V. Montana, G. Salama, and J. Y. Wu, "A naphthyl analog of the aminostyryl pyridinium class of potentiometric membrane dyes shows consistent sensitivity in a variety of tissue, cell, and model membrane preparations," J. Membrane Bio. 130, 1-10 (1992).

French, P. M. W.

D. M. Owen, P. M. P. Lanigan, C. Dunsby, I. Munro, D. Grant, M. A. A. Neil, P. M. W. French, and A. I. Magee, "Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells." Biophys. J. 90, L80 - L82 (2006).
[CrossRef] [PubMed]

French, P. M.W.

D. M. Owen, M. A.A. Neil, P. M.W. French and A. I. Magee, "Optical techniques for imaging membrane lipid microdomains in living cells," Semin. Cell Biol. 18, 591-598 (2007).
[CrossRef]

Fromherz, P.

P. Fromherz, K. H. Dambacher, H. Ephardt, A. Lambacher, C. O. Müller, R. Neigl, H. Schaden, O. Schenk, and T. Vetter, "Fluorescent dyes as probes of voltage transients in neuron membranes," Berichte der Bunsengesellschaft fuer Physikalische Chemie 95, 1333-1345 (1991).

Gerbeau-Pissot, P.

Y. Roche, P. Gerbeau-Pissot, B. Buhot, D. Thomas, L. Bonneau, J. Gresti, S Mongrand, J.-M. Perrier-Cornet, and F. Simon-Plas, "Depletion of phytosterols from the plant plasma membrane provides evidence for disruption of lipid rafts," The FASEB Journal 22, 1-12 (2008).
[CrossRef]

Goldsmith, P. C.

P. L. Mellon, J. J. Windle, P. C. Goldsmith, C. A. Padula, J. L. Roberts, and R. I. Weiner, "Immortalization of hypothalamic GnRH neurons by genetically targeted tumorigenesis," Neuron 5, 1-10 (1990).
[CrossRef] [PubMed]

Gould, E.

B. Leuner, E. Gould, and T. J. Shors, "Is there a link between adult neurogenesis and learning?" Hippocampus 16, 216-224 (2006).
[CrossRef] [PubMed]

Grant, D.

D. M. Owen, P. M. P. Lanigan, C. Dunsby, I. Munro, D. Grant, M. A. A. Neil, P. M. W. French, and A. I. Magee, "Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells." Biophys. J. 90, L80 - L82 (2006).
[CrossRef] [PubMed]

Gresti, J.

Y. Roche, P. Gerbeau-Pissot, B. Buhot, D. Thomas, L. Bonneau, J. Gresti, S Mongrand, J.-M. Perrier-Cornet, and F. Simon-Plas, "Depletion of phytosterols from the plant plasma membrane provides evidence for disruption of lipid rafts," The FASEB Journal 22, 1-12 (2008).
[CrossRef]

Jin, L.

L. Jin, A. C. Millard, J. P. Wuskell, X. Dong, D. Wu, H. A. Clark, and L. M. Loew, "Characterization and application of a new optical probe for membrane lipid domains," Biophys. J. 90, 2563-2575 (2006).
[CrossRef] [PubMed]

Kim, G.-H.

J. A. N. Fisher, J. R. Barchi, C. G. Welle, G.-H. Kim, P. Kosterin, A. L. Obaid, A. G. Yodh, D. Contreras and B. M. Salzberg, "Two-photon excitation of potentiometric probes enables optical recording of action potentials from mammalian nerve terminals in situ," J. Neurophysiol. 99, 1545-1553 (2008).
[CrossRef] [PubMed]

Kosterin, P.

J. A. N. Fisher, J. R. Barchi, C. G. Welle, G.-H. Kim, P. Kosterin, A. L. Obaid, A. G. Yodh, D. Contreras and B. M. Salzberg, "Two-photon excitation of potentiometric probes enables optical recording of action potentials from mammalian nerve terminals in situ," J. Neurophysiol. 99, 1545-1553 (2008).
[CrossRef] [PubMed]

Lambacher, A.

P. Fromherz, K. H. Dambacher, H. Ephardt, A. Lambacher, C. O. Müller, R. Neigl, H. Schaden, O. Schenk, and T. Vetter, "Fluorescent dyes as probes of voltage transients in neuron membranes," Berichte der Bunsengesellschaft fuer Physikalische Chemie 95, 1333-1345 (1991).

Lanigan, P. M. P.

D. M. Owen, P. M. P. Lanigan, C. Dunsby, I. Munro, D. Grant, M. A. A. Neil, P. M. W. French, and A. I. Magee, "Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells." Biophys. J. 90, L80 - L82 (2006).
[CrossRef] [PubMed]

Leuner, B.

B. Leuner, E. Gould, and T. J. Shors, "Is there a link between adult neurogenesis and learning?" Hippocampus 16, 216-224 (2006).
[CrossRef] [PubMed]

Lindstrom, J.

A. L. Obaid, M. E. Nelson, J. Lindstrom and B. M. Salzberg, "Optical studies of nicotinic acetylcholine receptor subtypes in the guinea-pig," J. Experimental Bio. 208, 2981-3001 (2005).
[CrossRef]

Liposits, Z.

Z. Liposits, I. Merchenthaler, W. C. Wetsel, J. J. Reid, P. L. Mellon, R. I. Weiner, and A. Negro-Vilar, "Morphological characterization of immortalized hypothalamic neurons synthesizing luteinizing hormone-releasing hormone," Endocrinology 129, 1575-1583 (1991).
[CrossRef] [PubMed]

Loew, L. M.

L. Jin, A. C. Millard, J. P. Wuskell, X. Dong, D. Wu, H. A. Clark, and L. M. Loew, "Characterization and application of a new optical probe for membrane lipid domains," Biophys. J. 90, 2563-2575 (2006).
[CrossRef] [PubMed]

A. L. Obaid, L. M. Loew, J. P. Wuskell, B. M. Salzberg, "Novel naphthylstyryl-pyridinium potentiometric dyes offer advantages for neural network analysis," J. Neurosci. Methods 134, 179-190 (2004).
[CrossRef] [PubMed]

L. M. Loew, "Potentiometric dyes: imaging electrical activity of cell membranes," Pure Appl. Chem. 68, 1405-1409 (1996).
[CrossRef]

L. M. Loew, L. B. Cohen, J. Dix, E. N. Fluhler, V. Montana, G. Salama, and J. Y. Wu, "A naphthyl analog of the aminostyryl pyridinium class of potentiometric membrane dyes shows consistent sensitivity in a variety of tissue, cell, and model membrane preparations," J. Membrane Bio. 130, 1-10 (1992).

Magee, A. I.

D. M. Owen, M. A.A. Neil, P. M.W. French and A. I. Magee, "Optical techniques for imaging membrane lipid microdomains in living cells," Semin. Cell Biol. 18, 591-598 (2007).
[CrossRef]

D. M. Owen, P. M. P. Lanigan, C. Dunsby, I. Munro, D. Grant, M. A. A. Neil, P. M. W. French, and A. I. Magee, "Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells." Biophys. J. 90, L80 - L82 (2006).
[CrossRef] [PubMed]

Mellon, P. L.

Z. Liposits, I. Merchenthaler, W. C. Wetsel, J. J. Reid, P. L. Mellon, R. I. Weiner, and A. Negro-Vilar, "Morphological characterization of immortalized hypothalamic neurons synthesizing luteinizing hormone-releasing hormone," Endocrinology 129, 1575-1583 (1991).
[CrossRef] [PubMed]

P. L. Mellon, J. J. Windle, P. C. Goldsmith, C. A. Padula, J. L. Roberts, and R. I. Weiner, "Immortalization of hypothalamic GnRH neurons by genetically targeted tumorigenesis," Neuron 5, 1-10 (1990).
[CrossRef] [PubMed]

Merchenthaler, I.

Z. Liposits, I. Merchenthaler, W. C. Wetsel, J. J. Reid, P. L. Mellon, R. I. Weiner, and A. Negro-Vilar, "Morphological characterization of immortalized hypothalamic neurons synthesizing luteinizing hormone-releasing hormone," Endocrinology 129, 1575-1583 (1991).
[CrossRef] [PubMed]

Millard, A. C.

L. Jin, A. C. Millard, J. P. Wuskell, X. Dong, D. Wu, H. A. Clark, and L. M. Loew, "Characterization and application of a new optical probe for membrane lipid domains," Biophys. J. 90, 2563-2575 (2006).
[CrossRef] [PubMed]

Mongrand, S

Y. Roche, P. Gerbeau-Pissot, B. Buhot, D. Thomas, L. Bonneau, J. Gresti, S Mongrand, J.-M. Perrier-Cornet, and F. Simon-Plas, "Depletion of phytosterols from the plant plasma membrane provides evidence for disruption of lipid rafts," The FASEB Journal 22, 1-12 (2008).
[CrossRef]

Montana, V.

L. M. Loew, L. B. Cohen, J. Dix, E. N. Fluhler, V. Montana, G. Salama, and J. Y. Wu, "A naphthyl analog of the aminostyryl pyridinium class of potentiometric membrane dyes shows consistent sensitivity in a variety of tissue, cell, and model membrane preparations," J. Membrane Bio. 130, 1-10 (1992).

Müller, C. O.

P. Fromherz, K. H. Dambacher, H. Ephardt, A. Lambacher, C. O. Müller, R. Neigl, H. Schaden, O. Schenk, and T. Vetter, "Fluorescent dyes as probes of voltage transients in neuron membranes," Berichte der Bunsengesellschaft fuer Physikalische Chemie 95, 1333-1345 (1991).

Munro, I.

D. M. Owen, P. M. P. Lanigan, C. Dunsby, I. Munro, D. Grant, M. A. A. Neil, P. M. W. French, and A. I. Magee, "Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells." Biophys. J. 90, L80 - L82 (2006).
[CrossRef] [PubMed]

Negro-Vilar, A.

Z. Liposits, I. Merchenthaler, W. C. Wetsel, J. J. Reid, P. L. Mellon, R. I. Weiner, and A. Negro-Vilar, "Morphological characterization of immortalized hypothalamic neurons synthesizing luteinizing hormone-releasing hormone," Endocrinology 129, 1575-1583 (1991).
[CrossRef] [PubMed]

Neigl, R.

P. Fromherz, K. H. Dambacher, H. Ephardt, A. Lambacher, C. O. Müller, R. Neigl, H. Schaden, O. Schenk, and T. Vetter, "Fluorescent dyes as probes of voltage transients in neuron membranes," Berichte der Bunsengesellschaft fuer Physikalische Chemie 95, 1333-1345 (1991).

Neil, M. A. A.

D. M. Owen, P. M. P. Lanigan, C. Dunsby, I. Munro, D. Grant, M. A. A. Neil, P. M. W. French, and A. I. Magee, "Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells." Biophys. J. 90, L80 - L82 (2006).
[CrossRef] [PubMed]

Neil, M. A.A.

D. M. Owen, M. A.A. Neil, P. M.W. French and A. I. Magee, "Optical techniques for imaging membrane lipid microdomains in living cells," Semin. Cell Biol. 18, 591-598 (2007).
[CrossRef]

Nelson, M. E.

A. L. Obaid, M. E. Nelson, J. Lindstrom and B. M. Salzberg, "Optical studies of nicotinic acetylcholine receptor subtypes in the guinea-pig," J. Experimental Bio. 208, 2981-3001 (2005).
[CrossRef]

Obaid, A. L.

J. A. N. Fisher, J. R. Barchi, C. G. Welle, G.-H. Kim, P. Kosterin, A. L. Obaid, A. G. Yodh, D. Contreras and B. M. Salzberg, "Two-photon excitation of potentiometric probes enables optical recording of action potentials from mammalian nerve terminals in situ," J. Neurophysiol. 99, 1545-1553 (2008).
[CrossRef] [PubMed]

A. L. Obaid, M. E. Nelson, J. Lindstrom and B. M. Salzberg, "Optical studies of nicotinic acetylcholine receptor subtypes in the guinea-pig," J. Experimental Bio. 208, 2981-3001 (2005).
[CrossRef]

A. L. Obaid, L. M. Loew, J. P. Wuskell, B. M. Salzberg, "Novel naphthylstyryl-pyridinium potentiometric dyes offer advantages for neural network analysis," J. Neurosci. Methods 134, 179-190 (2004).
[CrossRef] [PubMed]

Owen, D. M.

D. M. Owen, M. A.A. Neil, P. M.W. French and A. I. Magee, "Optical techniques for imaging membrane lipid microdomains in living cells," Semin. Cell Biol. 18, 591-598 (2007).
[CrossRef]

D. M. Owen, P. M. P. Lanigan, C. Dunsby, I. Munro, D. Grant, M. A. A. Neil, P. M. W. French, and A. I. Magee, "Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells." Biophys. J. 90, L80 - L82 (2006).
[CrossRef] [PubMed]

Padula, C. A.

P. L. Mellon, J. J. Windle, P. C. Goldsmith, C. A. Padula, J. L. Roberts, and R. I. Weiner, "Immortalization of hypothalamic GnRH neurons by genetically targeted tumorigenesis," Neuron 5, 1-10 (1990).
[CrossRef] [PubMed]

Perrier-Cornet, J.-M.

Y. Roche, P. Gerbeau-Pissot, B. Buhot, D. Thomas, L. Bonneau, J. Gresti, S Mongrand, J.-M. Perrier-Cornet, and F. Simon-Plas, "Depletion of phytosterols from the plant plasma membrane provides evidence for disruption of lipid rafts," The FASEB Journal 22, 1-12 (2008).
[CrossRef]

Price, P. J.

G. J. Brewer, J. R. Torricelli, E. K. Evege, and P. J. Price, "Optimized survival of hippocampal neurons in B27 -supplemented neurobasal, a new serum-free medium combination," J. Neurosci. Res. 35, 567-576 (1993).
[CrossRef] [PubMed]

Reid, J. J.

Z. Liposits, I. Merchenthaler, W. C. Wetsel, J. J. Reid, P. L. Mellon, R. I. Weiner, and A. Negro-Vilar, "Morphological characterization of immortalized hypothalamic neurons synthesizing luteinizing hormone-releasing hormone," Endocrinology 129, 1575-1583 (1991).
[CrossRef] [PubMed]

Roberts, J. L.

P. L. Mellon, J. J. Windle, P. C. Goldsmith, C. A. Padula, J. L. Roberts, and R. I. Weiner, "Immortalization of hypothalamic GnRH neurons by genetically targeted tumorigenesis," Neuron 5, 1-10 (1990).
[CrossRef] [PubMed]

Roche, Y.

Y. Roche, P. Gerbeau-Pissot, B. Buhot, D. Thomas, L. Bonneau, J. Gresti, S Mongrand, J.-M. Perrier-Cornet, and F. Simon-Plas, "Depletion of phytosterols from the plant plasma membrane provides evidence for disruption of lipid rafts," The FASEB Journal 22, 1-12 (2008).
[CrossRef]

Salama, G.

L. M. Loew, L. B. Cohen, J. Dix, E. N. Fluhler, V. Montana, G. Salama, and J. Y. Wu, "A naphthyl analog of the aminostyryl pyridinium class of potentiometric membrane dyes shows consistent sensitivity in a variety of tissue, cell, and model membrane preparations," J. Membrane Bio. 130, 1-10 (1992).

Salzberg, B. M.

J. A. N. Fisher, J. R. Barchi, C. G. Welle, G.-H. Kim, P. Kosterin, A. L. Obaid, A. G. Yodh, D. Contreras and B. M. Salzberg, "Two-photon excitation of potentiometric probes enables optical recording of action potentials from mammalian nerve terminals in situ," J. Neurophysiol. 99, 1545-1553 (2008).
[CrossRef] [PubMed]

A. L. Obaid, M. E. Nelson, J. Lindstrom and B. M. Salzberg, "Optical studies of nicotinic acetylcholine receptor subtypes in the guinea-pig," J. Experimental Bio. 208, 2981-3001 (2005).
[CrossRef]

A. L. Obaid, L. M. Loew, J. P. Wuskell, B. M. Salzberg, "Novel naphthylstyryl-pyridinium potentiometric dyes offer advantages for neural network analysis," J. Neurosci. Methods 134, 179-190 (2004).
[CrossRef] [PubMed]

Schaden, H.

P. Fromherz, K. H. Dambacher, H. Ephardt, A. Lambacher, C. O. Müller, R. Neigl, H. Schaden, O. Schenk, and T. Vetter, "Fluorescent dyes as probes of voltage transients in neuron membranes," Berichte der Bunsengesellschaft fuer Physikalische Chemie 95, 1333-1345 (1991).

Schenk, O.

P. Fromherz, K. H. Dambacher, H. Ephardt, A. Lambacher, C. O. Müller, R. Neigl, H. Schaden, O. Schenk, and T. Vetter, "Fluorescent dyes as probes of voltage transients in neuron membranes," Berichte der Bunsengesellschaft fuer Physikalische Chemie 95, 1333-1345 (1991).

Shors, T. J.

B. Leuner, E. Gould, and T. J. Shors, "Is there a link between adult neurogenesis and learning?" Hippocampus 16, 216-224 (2006).
[CrossRef] [PubMed]

Simon-Plas, F.

Y. Roche, P. Gerbeau-Pissot, B. Buhot, D. Thomas, L. Bonneau, J. Gresti, S Mongrand, J.-M. Perrier-Cornet, and F. Simon-Plas, "Depletion of phytosterols from the plant plasma membrane provides evidence for disruption of lipid rafts," The FASEB Journal 22, 1-12 (2008).
[CrossRef]

Thomas, D.

Y. Roche, P. Gerbeau-Pissot, B. Buhot, D. Thomas, L. Bonneau, J. Gresti, S Mongrand, J.-M. Perrier-Cornet, and F. Simon-Plas, "Depletion of phytosterols from the plant plasma membrane provides evidence for disruption of lipid rafts," The FASEB Journal 22, 1-12 (2008).
[CrossRef]

Torricelli, J. R.

G. J. Brewer, J. R. Torricelli, E. K. Evege, and P. J. Price, "Optimized survival of hippocampal neurons in B27 -supplemented neurobasal, a new serum-free medium combination," J. Neurosci. Res. 35, 567-576 (1993).
[CrossRef] [PubMed]

Vetter, T.

P. Fromherz, K. H. Dambacher, H. Ephardt, A. Lambacher, C. O. Müller, R. Neigl, H. Schaden, O. Schenk, and T. Vetter, "Fluorescent dyes as probes of voltage transients in neuron membranes," Berichte der Bunsengesellschaft fuer Physikalische Chemie 95, 1333-1345 (1991).

Wachowiak, M.

M. Djurisic, M. Zochowski, M. Wachowiak, C. X. Falk, L. B. Cohen, and D. Zecevic, "Optical monitoring of neural activity using voltage-sensitive dyes," Methods Enzymol. 361, 423-451 (2003).
[CrossRef] [PubMed]

Weiner, R. I.

Z. Liposits, I. Merchenthaler, W. C. Wetsel, J. J. Reid, P. L. Mellon, R. I. Weiner, and A. Negro-Vilar, "Morphological characterization of immortalized hypothalamic neurons synthesizing luteinizing hormone-releasing hormone," Endocrinology 129, 1575-1583 (1991).
[CrossRef] [PubMed]

P. L. Mellon, J. J. Windle, P. C. Goldsmith, C. A. Padula, J. L. Roberts, and R. I. Weiner, "Immortalization of hypothalamic GnRH neurons by genetically targeted tumorigenesis," Neuron 5, 1-10 (1990).
[CrossRef] [PubMed]

Welle, C. G.

J. A. N. Fisher, J. R. Barchi, C. G. Welle, G.-H. Kim, P. Kosterin, A. L. Obaid, A. G. Yodh, D. Contreras and B. M. Salzberg, "Two-photon excitation of potentiometric probes enables optical recording of action potentials from mammalian nerve terminals in situ," J. Neurophysiol. 99, 1545-1553 (2008).
[CrossRef] [PubMed]

Wetsel, W. C.

Z. Liposits, I. Merchenthaler, W. C. Wetsel, J. J. Reid, P. L. Mellon, R. I. Weiner, and A. Negro-Vilar, "Morphological characterization of immortalized hypothalamic neurons synthesizing luteinizing hormone-releasing hormone," Endocrinology 129, 1575-1583 (1991).
[CrossRef] [PubMed]

Windle, J. J.

P. L. Mellon, J. J. Windle, P. C. Goldsmith, C. A. Padula, J. L. Roberts, and R. I. Weiner, "Immortalization of hypothalamic GnRH neurons by genetically targeted tumorigenesis," Neuron 5, 1-10 (1990).
[CrossRef] [PubMed]

Wu, D.

L. Jin, A. C. Millard, J. P. Wuskell, X. Dong, D. Wu, H. A. Clark, and L. M. Loew, "Characterization and application of a new optical probe for membrane lipid domains," Biophys. J. 90, 2563-2575 (2006).
[CrossRef] [PubMed]

Wu, J. Y.

L. M. Loew, L. B. Cohen, J. Dix, E. N. Fluhler, V. Montana, G. Salama, and J. Y. Wu, "A naphthyl analog of the aminostyryl pyridinium class of potentiometric membrane dyes shows consistent sensitivity in a variety of tissue, cell, and model membrane preparations," J. Membrane Bio. 130, 1-10 (1992).

Wuskell, J. P.

L. Jin, A. C. Millard, J. P. Wuskell, X. Dong, D. Wu, H. A. Clark, and L. M. Loew, "Characterization and application of a new optical probe for membrane lipid domains," Biophys. J. 90, 2563-2575 (2006).
[CrossRef] [PubMed]

A. L. Obaid, L. M. Loew, J. P. Wuskell, B. M. Salzberg, "Novel naphthylstyryl-pyridinium potentiometric dyes offer advantages for neural network analysis," J. Neurosci. Methods 134, 179-190 (2004).
[CrossRef] [PubMed]

Yodh, A. G.

J. A. N. Fisher, J. R. Barchi, C. G. Welle, G.-H. Kim, P. Kosterin, A. L. Obaid, A. G. Yodh, D. Contreras and B. M. Salzberg, "Two-photon excitation of potentiometric probes enables optical recording of action potentials from mammalian nerve terminals in situ," J. Neurophysiol. 99, 1545-1553 (2008).
[CrossRef] [PubMed]

Zecevic, D.

M. Djurisic, M. Zochowski, M. Wachowiak, C. X. Falk, L. B. Cohen, and D. Zecevic, "Optical monitoring of neural activity using voltage-sensitive dyes," Methods Enzymol. 361, 423-451 (2003).
[CrossRef] [PubMed]

Zochowski, M.

M. Djurisic, M. Zochowski, M. Wachowiak, C. X. Falk, L. B. Cohen, and D. Zecevic, "Optical monitoring of neural activity using voltage-sensitive dyes," Methods Enzymol. 361, 423-451 (2003).
[CrossRef] [PubMed]

Berichte der Bunsengesellschaft fuer Physikalische Chemie (1)

P. Fromherz, K. H. Dambacher, H. Ephardt, A. Lambacher, C. O. Müller, R. Neigl, H. Schaden, O. Schenk, and T. Vetter, "Fluorescent dyes as probes of voltage transients in neuron membranes," Berichte der Bunsengesellschaft fuer Physikalische Chemie 95, 1333-1345 (1991).

Biophys. J. (2)

D. M. Owen, P. M. P. Lanigan, C. Dunsby, I. Munro, D. Grant, M. A. A. Neil, P. M. W. French, and A. I. Magee, "Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells." Biophys. J. 90, L80 - L82 (2006).
[CrossRef] [PubMed]

L. Jin, A. C. Millard, J. P. Wuskell, X. Dong, D. Wu, H. A. Clark, and L. M. Loew, "Characterization and application of a new optical probe for membrane lipid domains," Biophys. J. 90, 2563-2575 (2006).
[CrossRef] [PubMed]

Endocrinology (1)

Z. Liposits, I. Merchenthaler, W. C. Wetsel, J. J. Reid, P. L. Mellon, R. I. Weiner, and A. Negro-Vilar, "Morphological characterization of immortalized hypothalamic neurons synthesizing luteinizing hormone-releasing hormone," Endocrinology 129, 1575-1583 (1991).
[CrossRef] [PubMed]

Hippocampus (1)

B. Leuner, E. Gould, and T. J. Shors, "Is there a link between adult neurogenesis and learning?" Hippocampus 16, 216-224 (2006).
[CrossRef] [PubMed]

J. Experimental Bio. (1)

A. L. Obaid, M. E. Nelson, J. Lindstrom and B. M. Salzberg, "Optical studies of nicotinic acetylcholine receptor subtypes in the guinea-pig," J. Experimental Bio. 208, 2981-3001 (2005).
[CrossRef]

J. Membrane Bio. (1)

L. M. Loew, L. B. Cohen, J. Dix, E. N. Fluhler, V. Montana, G. Salama, and J. Y. Wu, "A naphthyl analog of the aminostyryl pyridinium class of potentiometric membrane dyes shows consistent sensitivity in a variety of tissue, cell, and model membrane preparations," J. Membrane Bio. 130, 1-10 (1992).

J. Neurophysiol. (1)

J. A. N. Fisher, J. R. Barchi, C. G. Welle, G.-H. Kim, P. Kosterin, A. L. Obaid, A. G. Yodh, D. Contreras and B. M. Salzberg, "Two-photon excitation of potentiometric probes enables optical recording of action potentials from mammalian nerve terminals in situ," J. Neurophysiol. 99, 1545-1553 (2008).
[CrossRef] [PubMed]

J. Neurosci. Methods (1)

A. L. Obaid, L. M. Loew, J. P. Wuskell, B. M. Salzberg, "Novel naphthylstyryl-pyridinium potentiometric dyes offer advantages for neural network analysis," J. Neurosci. Methods 134, 179-190 (2004).
[CrossRef] [PubMed]

J. Neurosci. Res. (1)

G. J. Brewer, J. R. Torricelli, E. K. Evege, and P. J. Price, "Optimized survival of hippocampal neurons in B27 -supplemented neurobasal, a new serum-free medium combination," J. Neurosci. Res. 35, 567-576 (1993).
[CrossRef] [PubMed]

Methods Enzymol. (1)

M. Djurisic, M. Zochowski, M. Wachowiak, C. X. Falk, L. B. Cohen, and D. Zecevic, "Optical monitoring of neural activity using voltage-sensitive dyes," Methods Enzymol. 361, 423-451 (2003).
[CrossRef] [PubMed]

Neuron (1)

P. L. Mellon, J. J. Windle, P. C. Goldsmith, C. A. Padula, J. L. Roberts, and R. I. Weiner, "Immortalization of hypothalamic GnRH neurons by genetically targeted tumorigenesis," Neuron 5, 1-10 (1990).
[CrossRef] [PubMed]

Pure Appl. Chem. (1)

L. M. Loew, "Potentiometric dyes: imaging electrical activity of cell membranes," Pure Appl. Chem. 68, 1405-1409 (1996).
[CrossRef]

Semin. Cell Biol. (1)

D. M. Owen, M. A.A. Neil, P. M.W. French and A. I. Magee, "Optical techniques for imaging membrane lipid microdomains in living cells," Semin. Cell Biol. 18, 591-598 (2007).
[CrossRef]

The FASEB Journal (1)

Y. Roche, P. Gerbeau-Pissot, B. Buhot, D. Thomas, L. Bonneau, J. Gresti, S Mongrand, J.-M. Perrier-Cornet, and F. Simon-Plas, "Depletion of phytosterols from the plant plasma membrane provides evidence for disruption of lipid rafts," The FASEB Journal 22, 1-12 (2008).
[CrossRef]

Other (4)

L. Jin, A. C. Millard, J. P. Wuskell, H. A. Clark, and L. M. Loew, "Cholesterol-enriched lipid domains can be visualized by di-4-ANEPPDHQ with linear and nonlinear optics," Biophys. J. 89, L04-L06 (2005).
[CrossRef] [PubMed]

B. Alberts, D. Bray, K. Hopkin, A. Johnson, J. Lewis, M. Raff, K. Roberts, and P. Walter, Essential Cell Biology, 2nd edition, (Garland Science, New York, 2003).

L. B. Cohen and B. M. Salzberg, "Optical measurement of membrane potential," Reviews of Physiology, Biochemistry and Pharmacology (Springer Berlin/Heidelberg), 83, 35-88 (1978).
[PubMed]

Invitrogen Corporation, "Potential-sensitive ANEP dyes," Data sheet, Revised: 24-March-2006, available at http://probes.invitrogen.com/media/pis/mp01199.pdf, November 5, 2008.

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Figures (4)

Fig. 1.
Fig. 1.

Chemical structure of the dye di-4-ANEPPDHQ [9]

Fig. 2.
Fig. 2.

Absorption spectra of the diluted stock solution (dye/DMSO), the staining solution (dye/HBSS), and stained primary hippocampal neurons and immortalized hypothalamic neurons. For comparison, the absorption spectra of DMSO and HBSS are also presented.

Fig. 3.
Fig. 3.

(a). Raw and (b) normalized excitation and emission spectra of the diluted stock and staining solutions, the stained primary hippocampal and immortalized hypothalamic neurons in HBSS before and after the addition of 1M KCl solution. (a) and (b) have the same legends.

Fig. 4.
Fig. 4.

phase-contrast (a, b) and fluorescence (c, d) images of stained primary hippocampal neurons (a, c) and immortalized hypothalamic neurons (b, d).

Tables (1)

Tables Icon

Table 1. Spectral characteristics of different solutions or targets tagged with ANEP dyes

Metrics