Abstract

Digital holographic microscopy allows determination of dynamic changes in the optical thickness profile of a transparent object with sub-wavelength accuracy. Here, we report a quantitative phase laser microsurgery system for evaluation of cellular/sub-cellular dynamic changes during laser micro-dissection. The proposed method takes advantage of the precise optical manipulation by the laser microbeam and quantitative phase imaging by digital holographic microscopy with high spatial and temporal resolution. This system will permit quantitative evaluation of the damage and/or the repair of the cell or cell organelles in real time.

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    [CrossRef]

2008 (2)

L. Yu, S. Mohanty, G. Liu, S. Genc, Z. Chen, and M. W. Berns, “Quantitative phase evaluation of dynamic changes on cell membrane during laser microsurgery,” J. Biomed. Opt. 13(5), 050508 (2008).
[CrossRef]

A. Khmaladze, M. K. Kim, and C. M. Lo, “Phase imaging of cells by simultaneous dual-wavelength reflection digital holography,” Opt. Express 16(15), 10900–10911 (2008), http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-15-10900 .
[CrossRef]

2007 (1)

M. W. Berns, “A history of laser scissors (microbeams),” Laser Manipulation of Cells and Tissues 82, 1–58 (2007).
[CrossRef]

2005 (2)

J. Colombelli, E. G. Reynaud, J. Rietdorf, R. Pepperkok, and E. H. K. Stelzer, “In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery,” Traffic 6(12), 1093–1102 (2005).
[CrossRef]

C. J. Mann, L. F. Yu, C. M. Lo, and M. K. Kim, “High-resolution quantitative phase-contrast microscopy by digital holography,” Opt. Express 13(22), 8693–8698 (2005), http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-13-22-8693 .
[CrossRef]

2004 (3)

2003 (1)

S. K. Mohanty, M. Sharma, and P. K. Gupta, “Laser-assisted microinjection into targeted animal cells,” Biotechnol. Lett. 25(11), 895–899 (2003).
[CrossRef]

2002 (2)

U. K. Tirlapur and K. König, “Femtosecond near-infrared laser pulses as a versatile non-invasive tool for intra-tissue nanoprocessing in plants without compromising viability,” Plant J. 31(3), 365–374 (2002).
[CrossRef]

H. Schneckenburger, A. Hendinger, R. Sailer, W. S. L. Strauss, and M. Schmitt, “Laser-assisted optoporation of single cells,” J. Biomed. Opt. 7(3), 410–416 (2002).
[CrossRef]

2001 (1)

Y. Shirahata, N. Ohkohchi, H. Itagak, and S. Satomi, “New technique for gene transfection using laser irradiation,” J. Investig. Med. 49(2), 184–190 (2001).
[CrossRef]

1999 (1)

1998 (2)

A. Barty, K. A. Nugent, D. Paganin, and A. Roberts, “Quantitative optical phase microscopy,” Opt. Lett. 23(11), 817–819 (1998), http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-23-11-817 .
[CrossRef]

C. E. Sims, G. D. Meredith, T. B. Krasieva, M. W. Berns, B. J. Tromberg, and N. L. Allbritton, “Laser-micropipet combination for single-cell analysis,” Anal. Chem. 70(21), 4570–4577 (1998).
[CrossRef]

1996 (1)

G. Palumbo, M. Caruso, E. Crescenzi, M. F. Tecce, G. Roberti, and A. Colasanti, “Targeted gene transfer in eucaryotic cells by dye-assisted laser optoporation,” J. Photochem. Photobiol. B 36(1), 41–46 (1996).
[CrossRef]

1992 (1)

Y. Tadir, J. Neev, and M. W. Berns, “Laser in assisted reproduction and genetics,” J. Assist. Reprod. Genet. 9(4), 303–305 (1992).
[CrossRef]

1988 (1)

R. M. Goldstein, H. A. Zebker, and C. L. Werner, “Satellite radar interferometry: Two-dimensional phase unwrapping,” Radio Sci. 23(4), 713–720 (1988).
[CrossRef]

1987 (2)

R. Wiegand, G. Weber, K. Zimmermann, S. Monajembashi, J. Wolfrum, and K. O. Greulich, “Laser-induced fusion of mammalian cells and plant protoplasts,” J. Cell Sci. 88(Pt 2), 145–149 (1987).

W. Tao, J. Wilkinson, E. J. Stanbridge, and M. W. Berns, “Direct gene transfer into human cultured cells facilitated by laser micropuncture of the cell membrane,” Proc. Natl. Acad. Sci. U.S.A. 84(12), 4180–4184 (1987).
[CrossRef]

1986 (1)

S. Monajembashi, C. Cremer, T. Cremer, J. Wolfrum, and K. O. Greulich, “Microdissection of human chromosomes by a laser microbeam,” Exp. Cell Res. 167(1), 262–265 (1986).
[CrossRef]

1981 (1)

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, “Laser microsurgery in cell and developmental biology,” Science 213(4507), 505–513 (1981).
[CrossRef]

1969 (2)

M. W. Berns, R. S. Olson, and D. E. Rounds, “In vitro production of chromosomal lesions with an argon laser microbeam,” Nature 221(5175), 74–75 (1969).
[CrossRef]

R. D. Allen, G. B. David, and G. Nomarski, “The Zeiss-Nomarski differential equipment for transmitted light microscopy,” Z. Wiss. Mikrosk. 69(4), 193–221 (1969).

Aist, J.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, “Laser microsurgery in cell and developmental biology,” Science 213(4507), 505–513 (1981).
[CrossRef]

Akkin, T.

Allbritton, N. L.

C. E. Sims, G. D. Meredith, T. B. Krasieva, M. W. Berns, B. J. Tromberg, and N. L. Allbritton, “Laser-micropipet combination for single-cell analysis,” Anal. Chem. 70(21), 4570–4577 (1998).
[CrossRef]

Allen, R. D.

R. D. Allen, G. B. David, and G. Nomarski, “The Zeiss-Nomarski differential equipment for transmitted light microscopy,” Z. Wiss. Mikrosk. 69(4), 193–221 (1969).

Badizadegan, K.

Barty, A.

Ben-Yakar, A.

M. F. Yanik, H. Cinar, H. N. Cinar, A. D. Chisholm, Y. Jin, and A. Ben-Yakar, “Neurosurgery: functional regeneration after laser axotomy,” Nature 432(7019), 822 (2004).
[CrossRef]

Berns, G. S.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, “Laser microsurgery in cell and developmental biology,” Science 213(4507), 505–513 (1981).
[CrossRef]

Berns, M. W.

L. Yu, S. Mohanty, G. Liu, S. Genc, Z. Chen, and M. W. Berns, “Quantitative phase evaluation of dynamic changes on cell membrane during laser microsurgery,” J. Biomed. Opt. 13(5), 050508 (2008).
[CrossRef]

M. W. Berns, “A history of laser scissors (microbeams),” Laser Manipulation of Cells and Tissues 82, 1–58 (2007).
[CrossRef]

C. E. Sims, G. D. Meredith, T. B. Krasieva, M. W. Berns, B. J. Tromberg, and N. L. Allbritton, “Laser-micropipet combination for single-cell analysis,” Anal. Chem. 70(21), 4570–4577 (1998).
[CrossRef]

Y. Tadir, J. Neev, and M. W. Berns, “Laser in assisted reproduction and genetics,” J. Assist. Reprod. Genet. 9(4), 303–305 (1992).
[CrossRef]

W. Tao, J. Wilkinson, E. J. Stanbridge, and M. W. Berns, “Direct gene transfer into human cultured cells facilitated by laser micropuncture of the cell membrane,” Proc. Natl. Acad. Sci. U.S.A. 84(12), 4180–4184 (1987).
[CrossRef]

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, “Laser microsurgery in cell and developmental biology,” Science 213(4507), 505–513 (1981).
[CrossRef]

M. W. Berns, R. S. Olson, and D. E. Rounds, “In vitro production of chromosomal lesions with an argon laser microbeam,” Nature 221(5175), 74–75 (1969).
[CrossRef]

Bevilacqua, F.

Brenner, S.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, “Laser microsurgery in cell and developmental biology,” Science 213(4507), 505–513 (1981).
[CrossRef]

Bryant, P. J.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, “Laser microsurgery in cell and developmental biology,” Science 213(4507), 505–513 (1981).
[CrossRef]

Burt, J.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, “Laser microsurgery in cell and developmental biology,” Science 213(4507), 505–513 (1981).
[CrossRef]

Cahill, T.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, “Laser microsurgery in cell and developmental biology,” Science 213(4507), 505–513 (1981).
[CrossRef]

Caruso, M.

G. Palumbo, M. Caruso, E. Crescenzi, M. F. Tecce, G. Roberti, and A. Colasanti, “Targeted gene transfer in eucaryotic cells by dye-assisted laser optoporation,” J. Photochem. Photobiol. B 36(1), 41–46 (1996).
[CrossRef]

Chen, Z.

L. Yu, S. Mohanty, G. Liu, S. Genc, Z. Chen, and M. W. Berns, “Quantitative phase evaluation of dynamic changes on cell membrane during laser microsurgery,” J. Biomed. Opt. 13(5), 050508 (2008).
[CrossRef]

Chisholm, A. D.

M. F. Yanik, H. Cinar, H. N. Cinar, A. D. Chisholm, Y. Jin, and A. Ben-Yakar, “Neurosurgery: functional regeneration after laser axotomy,” Nature 432(7019), 822 (2004).
[CrossRef]

Cinar, H.

M. F. Yanik, H. Cinar, H. N. Cinar, A. D. Chisholm, Y. Jin, and A. Ben-Yakar, “Neurosurgery: functional regeneration after laser axotomy,” Nature 432(7019), 822 (2004).
[CrossRef]

Cinar, H. N.

M. F. Yanik, H. Cinar, H. N. Cinar, A. D. Chisholm, Y. Jin, and A. Ben-Yakar, “Neurosurgery: functional regeneration after laser axotomy,” Nature 432(7019), 822 (2004).
[CrossRef]

Colasanti, A.

G. Palumbo, M. Caruso, E. Crescenzi, M. F. Tecce, G. Roberti, and A. Colasanti, “Targeted gene transfer in eucaryotic cells by dye-assisted laser optoporation,” J. Photochem. Photobiol. B 36(1), 41–46 (1996).
[CrossRef]

Colombelli, J.

J. Colombelli, E. G. Reynaud, J. Rietdorf, R. Pepperkok, and E. H. K. Stelzer, “In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery,” Traffic 6(12), 1093–1102 (2005).
[CrossRef]

Coulombe, J.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, “Laser microsurgery in cell and developmental biology,” Science 213(4507), 505–513 (1981).
[CrossRef]

Cremer, C.

S. Monajembashi, C. Cremer, T. Cremer, J. Wolfrum, and K. O. Greulich, “Microdissection of human chromosomes by a laser microbeam,” Exp. Cell Res. 167(1), 262–265 (1986).
[CrossRef]

Cremer, T.

S. Monajembashi, C. Cremer, T. Cremer, J. Wolfrum, and K. O. Greulich, “Microdissection of human chromosomes by a laser microbeam,” Exp. Cell Res. 167(1), 262–265 (1986).
[CrossRef]

Crescenzi, E.

G. Palumbo, M. Caruso, E. Crescenzi, M. F. Tecce, G. Roberti, and A. Colasanti, “Targeted gene transfer in eucaryotic cells by dye-assisted laser optoporation,” J. Photochem. Photobiol. B 36(1), 41–46 (1996).
[CrossRef]

Cuche, E.

Dasari, R. R.

Davé, D. P.

David, G. B.

R. D. Allen, G. B. David, and G. Nomarski, “The Zeiss-Nomarski differential equipment for transmitted light microscopy,” Z. Wiss. Mikrosk. 69(4), 193–221 (1969).

Deflores, L. P.

Depeursinge, C.

Diller, K. R.

Edwards, J.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, “Laser microsurgery in cell and developmental biology,” Science 213(4507), 505–513 (1981).
[CrossRef]

Feld, M. S.

Genc, S.

L. Yu, S. Mohanty, G. Liu, S. Genc, Z. Chen, and M. W. Berns, “Quantitative phase evaluation of dynamic changes on cell membrane during laser microsurgery,” J. Biomed. Opt. 13(5), 050508 (2008).
[CrossRef]

Girton, J.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, “Laser microsurgery in cell and developmental biology,” Science 213(4507), 505–513 (1981).
[CrossRef]

Goldstein, R. M.

R. M. Goldstein, H. A. Zebker, and C. L. Werner, “Satellite radar interferometry: Two-dimensional phase unwrapping,” Radio Sci. 23(4), 713–720 (1988).
[CrossRef]

Greulich, K. O.

R. Wiegand, G. Weber, K. Zimmermann, S. Monajembashi, J. Wolfrum, and K. O. Greulich, “Laser-induced fusion of mammalian cells and plant protoplasts,” J. Cell Sci. 88(Pt 2), 145–149 (1987).

S. Monajembashi, C. Cremer, T. Cremer, J. Wolfrum, and K. O. Greulich, “Microdissection of human chromosomes by a laser microbeam,” Exp. Cell Res. 167(1), 262–265 (1986).
[CrossRef]

Gupta, P. K.

S. K. Mohanty, M. Sharma, and P. K. Gupta, “Laser-assisted microinjection into targeted animal cells,” Biotechnol. Lett. 25(11), 895–899 (2003).
[CrossRef]

Hammer-Wilson, M.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, “Laser microsurgery in cell and developmental biology,” Science 213(4507), 505–513 (1981).
[CrossRef]

Hendinger, A.

H. Schneckenburger, A. Hendinger, R. Sailer, W. S. L. Strauss, and M. Schmitt, “Laser-assisted optoporation of single cells,” J. Biomed. Opt. 7(3), 410–416 (2002).
[CrossRef]

Itagak, H.

Y. Shirahata, N. Ohkohchi, H. Itagak, and S. Satomi, “New technique for gene transfection using laser irradiation,” J. Investig. Med. 49(2), 184–190 (2001).
[CrossRef]

Iwai, H.

Jin, Y.

M. F. Yanik, H. Cinar, H. N. Cinar, A. D. Chisholm, Y. Jin, and A. Ben-Yakar, “Neurosurgery: functional regeneration after laser axotomy,” Nature 432(7019), 822 (2004).
[CrossRef]

Khmaladze, A.

Kim, M. K.

Kitzes, M.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, “Laser microsurgery in cell and developmental biology,” Science 213(4507), 505–513 (1981).
[CrossRef]

König, K.

U. K. Tirlapur and K. König, “Femtosecond near-infrared laser pulses as a versatile non-invasive tool for intra-tissue nanoprocessing in plants without compromising viability,” Plant J. 31(3), 365–374 (2002).
[CrossRef]

Koonce, M.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, “Laser microsurgery in cell and developmental biology,” Science 213(4507), 505–513 (1981).
[CrossRef]

Krasieva, T. B.

C. E. Sims, G. D. Meredith, T. B. Krasieva, M. W. Berns, B. J. Tromberg, and N. L. Allbritton, “Laser-micropipet combination for single-cell analysis,” Anal. Chem. 70(21), 4570–4577 (1998).
[CrossRef]

Liaw, L. H.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, “Laser microsurgery in cell and developmental biology,” Science 213(4507), 505–513 (1981).
[CrossRef]

Liu, G.

L. Yu, S. Mohanty, G. Liu, S. Genc, Z. Chen, and M. W. Berns, “Quantitative phase evaluation of dynamic changes on cell membrane during laser microsurgery,” J. Biomed. Opt. 13(5), 050508 (2008).
[CrossRef]

Lo, C. M.

Mann, C. J.

McNeill, P.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, “Laser microsurgery in cell and developmental biology,” Science 213(4507), 505–513 (1981).
[CrossRef]

Meredith, G. D.

C. E. Sims, G. D. Meredith, T. B. Krasieva, M. W. Berns, B. J. Tromberg, and N. L. Allbritton, “Laser-micropipet combination for single-cell analysis,” Anal. Chem. 70(21), 4570–4577 (1998).
[CrossRef]

Milner, T. E.

Mohanty, S.

L. Yu, S. Mohanty, G. Liu, S. Genc, Z. Chen, and M. W. Berns, “Quantitative phase evaluation of dynamic changes on cell membrane during laser microsurgery,” J. Biomed. Opt. 13(5), 050508 (2008).
[CrossRef]

Mohanty, S. K.

S. K. Mohanty, M. Sharma, and P. K. Gupta, “Laser-assisted microinjection into targeted animal cells,” Biotechnol. Lett. 25(11), 895–899 (2003).
[CrossRef]

Monajembashi, S.

R. Wiegand, G. Weber, K. Zimmermann, S. Monajembashi, J. Wolfrum, and K. O. Greulich, “Laser-induced fusion of mammalian cells and plant protoplasts,” J. Cell Sci. 88(Pt 2), 145–149 (1987).

S. Monajembashi, C. Cremer, T. Cremer, J. Wolfrum, and K. O. Greulich, “Microdissection of human chromosomes by a laser microbeam,” Exp. Cell Res. 167(1), 262–265 (1986).
[CrossRef]

Neev, J.

Y. Tadir, J. Neev, and M. W. Berns, “Laser in assisted reproduction and genetics,” J. Assist. Reprod. Genet. 9(4), 303–305 (1992).
[CrossRef]

Nomarski, G.

R. D. Allen, G. B. David, and G. Nomarski, “The Zeiss-Nomarski differential equipment for transmitted light microscopy,” Z. Wiss. Mikrosk. 69(4), 193–221 (1969).

Nugent, K. A.

Ohkohchi, N.

Y. Shirahata, N. Ohkohchi, H. Itagak, and S. Satomi, “New technique for gene transfection using laser irradiation,” J. Investig. Med. 49(2), 184–190 (2001).
[CrossRef]

Olson, R. S.

M. W. Berns, R. S. Olson, and D. E. Rounds, “In vitro production of chromosomal lesions with an argon laser microbeam,” Nature 221(5175), 74–75 (1969).
[CrossRef]

Paganin, D.

Palumbo, G.

G. Palumbo, M. Caruso, E. Crescenzi, M. F. Tecce, G. Roberti, and A. Colasanti, “Targeted gene transfer in eucaryotic cells by dye-assisted laser optoporation,” J. Photochem. Photobiol. B 36(1), 41–46 (1996).
[CrossRef]

Pepperkok, R.

J. Colombelli, E. G. Reynaud, J. Rietdorf, R. Pepperkok, and E. H. K. Stelzer, “In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery,” Traffic 6(12), 1093–1102 (2005).
[CrossRef]

Peterson, S.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, “Laser microsurgery in cell and developmental biology,” Science 213(4507), 505–513 (1981).
[CrossRef]

Popescu, G.

Rattner, J. B.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, “Laser microsurgery in cell and developmental biology,” Science 213(4507), 505–513 (1981).
[CrossRef]

Reynaud, E. G.

J. Colombelli, E. G. Reynaud, J. Rietdorf, R. Pepperkok, and E. H. K. Stelzer, “In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery,” Traffic 6(12), 1093–1102 (2005).
[CrossRef]

Rietdorf, J.

J. Colombelli, E. G. Reynaud, J. Rietdorf, R. Pepperkok, and E. H. K. Stelzer, “In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery,” Traffic 6(12), 1093–1102 (2005).
[CrossRef]

Roberti, G.

G. Palumbo, M. Caruso, E. Crescenzi, M. F. Tecce, G. Roberti, and A. Colasanti, “Targeted gene transfer in eucaryotic cells by dye-assisted laser optoporation,” J. Photochem. Photobiol. B 36(1), 41–46 (1996).
[CrossRef]

Roberts, A.

Rounds, D. E.

M. W. Berns, R. S. Olson, and D. E. Rounds, “In vitro production of chromosomal lesions with an argon laser microbeam,” Nature 221(5175), 74–75 (1969).
[CrossRef]

Rylander, C. G.

Sailer, R.

H. Schneckenburger, A. Hendinger, R. Sailer, W. S. L. Strauss, and M. Schmitt, “Laser-assisted optoporation of single cells,” J. Biomed. Opt. 7(3), 410–416 (2002).
[CrossRef]

Satomi, S.

Y. Shirahata, N. Ohkohchi, H. Itagak, and S. Satomi, “New technique for gene transfection using laser irradiation,” J. Investig. Med. 49(2), 184–190 (2001).
[CrossRef]

Schmitt, M.

H. Schneckenburger, A. Hendinger, R. Sailer, W. S. L. Strauss, and M. Schmitt, “Laser-assisted optoporation of single cells,” J. Biomed. Opt. 7(3), 410–416 (2002).
[CrossRef]

Schneckenburger, H.

H. Schneckenburger, A. Hendinger, R. Sailer, W. S. L. Strauss, and M. Schmitt, “Laser-assisted optoporation of single cells,” J. Biomed. Opt. 7(3), 410–416 (2002).
[CrossRef]

Sharma, M.

S. K. Mohanty, M. Sharma, and P. K. Gupta, “Laser-assisted microinjection into targeted animal cells,” Biotechnol. Lett. 25(11), 895–899 (2003).
[CrossRef]

Shirahata, Y.

Y. Shirahata, N. Ohkohchi, H. Itagak, and S. Satomi, “New technique for gene transfection using laser irradiation,” J. Investig. Med. 49(2), 184–190 (2001).
[CrossRef]

Siemens, A.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, “Laser microsurgery in cell and developmental biology,” Science 213(4507), 505–513 (1981).
[CrossRef]

Sims, C. E.

C. E. Sims, G. D. Meredith, T. B. Krasieva, M. W. Berns, B. J. Tromberg, and N. L. Allbritton, “Laser-micropipet combination for single-cell analysis,” Anal. Chem. 70(21), 4570–4577 (1998).
[CrossRef]

Stanbridge, E. J.

W. Tao, J. Wilkinson, E. J. Stanbridge, and M. W. Berns, “Direct gene transfer into human cultured cells facilitated by laser micropuncture of the cell membrane,” Proc. Natl. Acad. Sci. U.S.A. 84(12), 4180–4184 (1987).
[CrossRef]

Stelzer, E. H. K.

J. Colombelli, E. G. Reynaud, J. Rietdorf, R. Pepperkok, and E. H. K. Stelzer, “In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery,” Traffic 6(12), 1093–1102 (2005).
[CrossRef]

Strahs, K.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, “Laser microsurgery in cell and developmental biology,” Science 213(4507), 505–513 (1981).
[CrossRef]

Strauss, W. S. L.

H. Schneckenburger, A. Hendinger, R. Sailer, W. S. L. Strauss, and M. Schmitt, “Laser-assisted optoporation of single cells,” J. Biomed. Opt. 7(3), 410–416 (2002).
[CrossRef]

Tadir, Y.

Y. Tadir, J. Neev, and M. W. Berns, “Laser in assisted reproduction and genetics,” J. Assist. Reprod. Genet. 9(4), 303–305 (1992).
[CrossRef]

Tao, W.

W. Tao, J. Wilkinson, E. J. Stanbridge, and M. W. Berns, “Direct gene transfer into human cultured cells facilitated by laser micropuncture of the cell membrane,” Proc. Natl. Acad. Sci. U.S.A. 84(12), 4180–4184 (1987).
[CrossRef]

Tecce, M. F.

G. Palumbo, M. Caruso, E. Crescenzi, M. F. Tecce, G. Roberti, and A. Colasanti, “Targeted gene transfer in eucaryotic cells by dye-assisted laser optoporation,” J. Photochem. Photobiol. B 36(1), 41–46 (1996).
[CrossRef]

Tirlapur, U. K.

U. K. Tirlapur and K. König, “Femtosecond near-infrared laser pulses as a versatile non-invasive tool for intra-tissue nanoprocessing in plants without compromising viability,” Plant J. 31(3), 365–374 (2002).
[CrossRef]

Tromberg, B. J.

C. E. Sims, G. D. Meredith, T. B. Krasieva, M. W. Berns, B. J. Tromberg, and N. L. Allbritton, “Laser-micropipet combination for single-cell analysis,” Anal. Chem. 70(21), 4570–4577 (1998).
[CrossRef]

van Dyk, D.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, “Laser microsurgery in cell and developmental biology,” Science 213(4507), 505–513 (1981).
[CrossRef]

Vaughan, J. C.

Walter, R.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, “Laser microsurgery in cell and developmental biology,” Science 213(4507), 505–513 (1981).
[CrossRef]

Weber, G.

R. Wiegand, G. Weber, K. Zimmermann, S. Monajembashi, J. Wolfrum, and K. O. Greulich, “Laser-induced fusion of mammalian cells and plant protoplasts,” J. Cell Sci. 88(Pt 2), 145–149 (1987).

Welch, A. J.

Werner, C. L.

R. M. Goldstein, H. A. Zebker, and C. L. Werner, “Satellite radar interferometry: Two-dimensional phase unwrapping,” Radio Sci. 23(4), 713–720 (1988).
[CrossRef]

Wiegand, R.

R. Wiegand, G. Weber, K. Zimmermann, S. Monajembashi, J. Wolfrum, and K. O. Greulich, “Laser-induced fusion of mammalian cells and plant protoplasts,” J. Cell Sci. 88(Pt 2), 145–149 (1987).

Wilkinson, J.

W. Tao, J. Wilkinson, E. J. Stanbridge, and M. W. Berns, “Direct gene transfer into human cultured cells facilitated by laser micropuncture of the cell membrane,” Proc. Natl. Acad. Sci. U.S.A. 84(12), 4180–4184 (1987).
[CrossRef]

Wolfrum, J.

R. Wiegand, G. Weber, K. Zimmermann, S. Monajembashi, J. Wolfrum, and K. O. Greulich, “Laser-induced fusion of mammalian cells and plant protoplasts,” J. Cell Sci. 88(Pt 2), 145–149 (1987).

S. Monajembashi, C. Cremer, T. Cremer, J. Wolfrum, and K. O. Greulich, “Microdissection of human chromosomes by a laser microbeam,” Exp. Cell Res. 167(1), 262–265 (1986).
[CrossRef]

Yanik, M. F.

M. F. Yanik, H. Cinar, H. N. Cinar, A. D. Chisholm, Y. Jin, and A. Ben-Yakar, “Neurosurgery: functional regeneration after laser axotomy,” Nature 432(7019), 822 (2004).
[CrossRef]

Yu, L.

L. Yu, S. Mohanty, G. Liu, S. Genc, Z. Chen, and M. W. Berns, “Quantitative phase evaluation of dynamic changes on cell membrane during laser microsurgery,” J. Biomed. Opt. 13(5), 050508 (2008).
[CrossRef]

Yu, L. F.

Zebker, H. A.

R. M. Goldstein, H. A. Zebker, and C. L. Werner, “Satellite radar interferometry: Two-dimensional phase unwrapping,” Radio Sci. 23(4), 713–720 (1988).
[CrossRef]

Zimmermann, K.

R. Wiegand, G. Weber, K. Zimmermann, S. Monajembashi, J. Wolfrum, and K. O. Greulich, “Laser-induced fusion of mammalian cells and plant protoplasts,” J. Cell Sci. 88(Pt 2), 145–149 (1987).

Anal. Chem. (1)

C. E. Sims, G. D. Meredith, T. B. Krasieva, M. W. Berns, B. J. Tromberg, and N. L. Allbritton, “Laser-micropipet combination for single-cell analysis,” Anal. Chem. 70(21), 4570–4577 (1998).
[CrossRef]

Biotechnol. Lett. (1)

S. K. Mohanty, M. Sharma, and P. K. Gupta, “Laser-assisted microinjection into targeted animal cells,” Biotechnol. Lett. 25(11), 895–899 (2003).
[CrossRef]

Exp. Cell Res. (1)

S. Monajembashi, C. Cremer, T. Cremer, J. Wolfrum, and K. O. Greulich, “Microdissection of human chromosomes by a laser microbeam,” Exp. Cell Res. 167(1), 262–265 (1986).
[CrossRef]

J. Assist. Reprod. Genet. (1)

Y. Tadir, J. Neev, and M. W. Berns, “Laser in assisted reproduction and genetics,” J. Assist. Reprod. Genet. 9(4), 303–305 (1992).
[CrossRef]

J. Biomed. Opt. (2)

H. Schneckenburger, A. Hendinger, R. Sailer, W. S. L. Strauss, and M. Schmitt, “Laser-assisted optoporation of single cells,” J. Biomed. Opt. 7(3), 410–416 (2002).
[CrossRef]

L. Yu, S. Mohanty, G. Liu, S. Genc, Z. Chen, and M. W. Berns, “Quantitative phase evaluation of dynamic changes on cell membrane during laser microsurgery,” J. Biomed. Opt. 13(5), 050508 (2008).
[CrossRef]

J. Cell Sci. (1)

R. Wiegand, G. Weber, K. Zimmermann, S. Monajembashi, J. Wolfrum, and K. O. Greulich, “Laser-induced fusion of mammalian cells and plant protoplasts,” J. Cell Sci. 88(Pt 2), 145–149 (1987).

J. Investig. Med. (1)

Y. Shirahata, N. Ohkohchi, H. Itagak, and S. Satomi, “New technique for gene transfection using laser irradiation,” J. Investig. Med. 49(2), 184–190 (2001).
[CrossRef]

J. Photochem. Photobiol. B (1)

G. Palumbo, M. Caruso, E. Crescenzi, M. F. Tecce, G. Roberti, and A. Colasanti, “Targeted gene transfer in eucaryotic cells by dye-assisted laser optoporation,” J. Photochem. Photobiol. B 36(1), 41–46 (1996).
[CrossRef]

Laser Manipulation of Cells and Tissues (1)

M. W. Berns, “A history of laser scissors (microbeams),” Laser Manipulation of Cells and Tissues 82, 1–58 (2007).
[CrossRef]

Nature (2)

M. W. Berns, R. S. Olson, and D. E. Rounds, “In vitro production of chromosomal lesions with an argon laser microbeam,” Nature 221(5175), 74–75 (1969).
[CrossRef]

M. F. Yanik, H. Cinar, H. N. Cinar, A. D. Chisholm, Y. Jin, and A. Ben-Yakar, “Neurosurgery: functional regeneration after laser axotomy,” Nature 432(7019), 822 (2004).
[CrossRef]

Opt. Express (2)

Opt. Lett. (4)

Plant J. (1)

U. K. Tirlapur and K. König, “Femtosecond near-infrared laser pulses as a versatile non-invasive tool for intra-tissue nanoprocessing in plants without compromising viability,” Plant J. 31(3), 365–374 (2002).
[CrossRef]

Proc. Natl. Acad. Sci. U.S.A. (1)

W. Tao, J. Wilkinson, E. J. Stanbridge, and M. W. Berns, “Direct gene transfer into human cultured cells facilitated by laser micropuncture of the cell membrane,” Proc. Natl. Acad. Sci. U.S.A. 84(12), 4180–4184 (1987).
[CrossRef]

Radio Sci. (1)

R. M. Goldstein, H. A. Zebker, and C. L. Werner, “Satellite radar interferometry: Two-dimensional phase unwrapping,” Radio Sci. 23(4), 713–720 (1988).
[CrossRef]

Science (1)

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, “Laser microsurgery in cell and developmental biology,” Science 213(4507), 505–513 (1981).
[CrossRef]

Traffic (1)

J. Colombelli, E. G. Reynaud, J. Rietdorf, R. Pepperkok, and E. H. K. Stelzer, “In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery,” Traffic 6(12), 1093–1102 (2005).
[CrossRef]

Z. Wiss. Mikrosk. (1)

R. D. Allen, G. B. David, and G. Nomarski, “The Zeiss-Nomarski differential equipment for transmitted light microscopy,” Z. Wiss. Mikrosk. 69(4), 193–221 (1969).

Other (2)

J. W. Goodman, Introduction to Fourier Optics (McGraw-Hill, 1996).

K. F. A. Ross, Phase Contrast and Interference Microscopy for Cell Biologists (Edward Arnold Publishers, London, 1967).

Supplementary Material (5)

» Media 1: MOV (2409 KB)     
» Media 2: MOV (2388 KB)     
» Media 3: MOV (4751 KB)     
» Media 4: MOV (319 KB)     
» Media 5: MOV (3145 KB)     

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Figures (5)

Fig. 1.
Fig. 1.

Schematic of the experimental setup. LD is the 675nm laser diode; BS1 and BS2 are beam splitters; Ms are mirrors; S is a shutter; L is a lens; HPF is a high-pass filter for red light.

Fig. 2.
Fig. 2.

(Color) Bright-field images of rat kangaroo kidney epithelial (PTK2) cells: (a) Before the laser microbeam; (b) 30 seconds after the laser microbeam; (c) 5 min after the laser microbeam. Figure (d) shows several time-lapse quantitative phase images during the laser microbeam. A time-lapse movie (Media 1) is shown in (f). Panel (e) shows several time-lapse phase changes of the cell compared to its original status before laser microsurgery and Fig. (g) shows the corresponding movie (Media 2). Scale bar: 10 µm, n=1.38.

Fig. 3.
Fig. 3.

Bright-field images of PTK2 cell nucleolus: (a) before laser micro-dissection; (b) during laser micro-dissection, with the targeting laser beam on (and passed to the camera) showing the micro-focused spot; (c) after laser micro-irradiation. Figure (d) shows a time-lapse sequence of quantitative phase images (Media 3) during laser microbeam. Scale bar: 10 µm.

Fig. 4.
Fig. 4.

(Color) (a) Bright-field image of crenate RBC cells; and (b) time-lapse quantitative phase maps of the RBCs during laser microirradiation. Figure (c) shows a movie of quantitative phase images before and during laser microbeam (Media 4). Assuming n=1.43 for crenate RBCs.

Fig. 5.
Fig. 5.

(Color) Bright-field images of the rod cell from Goldfish retina: (a) Before the laser microbeam; (b) after the laser microbeam; Panel (c) shows quantitative phase change during laser microirradiation (Media 5). Scale bar: 10 µm, n=1.38.

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