Abstract

The main mechanism responsible for cell motility is the stochastic generation and breakup of actin filaments forming the cytoskeleton. However, the role of environmental factors in the migration and differentiation of cells is yet to be fully understood. Here we demonstrate that polarized optical fields can exert controllable torques on the actin network and therefore influence the treadmilling process responsible for cells motility. Through systematic experiments and stochastic modeling we demonstrate that actively guiding the dynamics of large groups of cells is possible in a noninvasive manner.

© 2009 Optical Society of America

Full Article  |  PDF Article

References

  • View by:
  • |
  • |
  • |

  1. T. Betz, D. Lim, and J. A. Käs, "Neuronal Growth: a bistable stochastic process," Phys. Rev. Lett. 96, 098103 (2006).
    [CrossRef] [PubMed]
  2. K. Keren, Z. Pincus, G. M. Allen, E. L. Barnhart, G. Marriott, A. Mogilner, and J. A. Theriot, "Mechanism of shape determination in motile cells," Nature 453, 475-480 (2008).
    [CrossRef] [PubMed]
  3. H. Lodish, A. Berk, S. L. Zipursky, P. Matsudaira, D. Baltimore, and J. E. Darnell, Molecular Cell Biology (W. H. Freeman, New York, 2004).
  4. A. Ashkin, "Acceleration and trapping of particles by radiation pressure," Phys. Rev. Lett. 24, 156-159 (1970).
    [CrossRef]
  5. A. Ashkin and J. M. Dziedzik, "Optical trapping and manipulation of viruses and bacteria," Science 235, 1517-1520 (1987).
    [CrossRef] [PubMed]
  6. Y. Roichman, B. Sun, Y. Roichman, J. Amato-Grill, and D. G. Grier, "Optical forces arising from phase gradients," Phys. Rev. Lett. 100, 013602 (2008).
    [CrossRef] [PubMed]
  7. S. Albaladejo, M. I. Marqués, M. Laroche, and J. J. Sáenz, "Scattering forces from the curl of the spin angular momentum of a light field," Phys. Rev. Lett. 102, 113602 (2009).
    [CrossRef] [PubMed]
  8. R. A. Beth, "Mechanical detection and measurement of the angular momentum of light," Phys. Rev. 50, 115-125 (1936).
    [CrossRef]
  9. L. Allen, S. M. Barnett, M. J. Padgett, Optical Angular Momentum (Institute of Physics Pub., Bristol, 2003).
    [CrossRef]
  10. K. Svoboda and S. M. Block, "Biological Applications of optical forces," Annu. Rev. Biophys. Biomol. Struct. 23, 247-285 (1994).
    [CrossRef] [PubMed]
  11. D. J. Odde and M. J. Renn, "Laser-guided direct writing of living cells," Biotechnol. Bioeng. 67, 312-318 (2000).
    [CrossRef] [PubMed]
  12. J. A. Dharmadhikari, S. Roy, A. K. Dharmadhikari, S. Sharma, and D. Mathur, "Torque-generating malaria-infected red blood cells in optical trap," Opt. Express 12, 1179-1184 (2004).
    [CrossRef] [PubMed]
  13. S. Bayoudh, T. A. Nieminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, "Orientation of biological cells using plane polarization Gaussian beam optical tweezers," J. Mod. Opt. 50, 1581-1590 (2003).
  14. G. Garab, P. Galajda, I. Pomozi, L. Finzi, T. Praznovszky, P. Ormos, and H. van Amerongen, "Alignment of biological microparticles by a polarized laser beam," Eur. Biophys. J. 34, 335-343 (2005).
    [CrossRef] [PubMed]
  15. A. Ehrlicher, T. Betz, B. Stuhrmann, D. Koch, V. Milner, M. G. Raizen, and J. A. Käs, "Guiding neuronal growth with light," Proc. Natl. Acad. Sci. 99, 16024-16028 (2002).
    [CrossRef] [PubMed]
  16. D. R. Burnham, G. D. Wright, N. D. Read, and D. McGloin, "Holographic and single beam optical manipulation of hyphal growth in filamentous fungi," J. Opt. A: Pure Appl. Opt. 9, S172-S179 (2007).
    [CrossRef]
  17. R. Ananthakrishnan and A. Ehrlicher, "The forces behind cell movement," Int. J. Biol. Sci. 3, 303-317 (2007).
    [PubMed]
  18. D. J. Carnegie, D. J. Stevenson, M. Mazilu, F. Gunn-Moore, and K. Dholakia, "Guided neuronal growth using optical line traps," Opt. Express 16, 10507-10517 (2008).
    [CrossRef] [PubMed]
  19. A. I. Norman, R. Ivkov, J. G. Forbes, and S. C. Greer, "The polymerization of actin: structural changes from small-angle neutron scattering," J. Chem. Phys. 123, 154904 (2005).
    [CrossRef] [PubMed]
  20. B. Bhaduri, A. Neild, and T. W. Ng, "Directional Brownian diffusion dynamics with variable magnitudes," Appl. Phys. Lett. 92, 084105 (2008).
    [CrossRef]
  21. A. I. Bishop, T. A. Nieminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, "Optical applcation and measurement of torque on microparticles of isotropic nonabsorbing material," Phys. Rev. A 68, 33802 (2003).
    [CrossRef]
  22. K. D. Bonin, B. Kourmanov, and T. G. Walker, "Light torque nanocontrol, nanomotors and nanorockers," Opt. Express 10, 984-989 (2002).
    [PubMed]
  23. A. La Porta and M. D. Wang, "Optical torque wrench: angular trapping, rotation, and torque detection of quartz microparticles," Phys. Rev. Lett. 92, 190801 (2004).
    [CrossRef] [PubMed]
  24. M. E. Friese, T. A. Nieminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, "Optical alignment and spinning of laser-trapped microscopic particles," Nature 394, 348-350 (1998).
    [CrossRef]
  25. M. Poujade, E. Grasland-Mongrain, A. Hertzog, J. Jouanneau, P. Chavrier, B. Ladoux, A. Buguin, and P. Silberzan, "Collective migration of an epithelial monolayer in response to a model wound," Proc. Natl. Acad. Sci. USA 104, 15988-15993 (2007).
    [CrossRef]
  26. A. Bianco, M. Poukkula, A. Cliffe, J. Mathieu, C. M. Luque, T. A. Fulga, and P. Rørth, "Two distinct modes of guidance signaling during collective migration of border cells," Nature 448, 362-365 (2007).
    [CrossRef] [PubMed]

2009

S. Albaladejo, M. I. Marqués, M. Laroche, and J. J. Sáenz, "Scattering forces from the curl of the spin angular momentum of a light field," Phys. Rev. Lett. 102, 113602 (2009).
[CrossRef] [PubMed]

2008

Y. Roichman, B. Sun, Y. Roichman, J. Amato-Grill, and D. G. Grier, "Optical forces arising from phase gradients," Phys. Rev. Lett. 100, 013602 (2008).
[CrossRef] [PubMed]

K. Keren, Z. Pincus, G. M. Allen, E. L. Barnhart, G. Marriott, A. Mogilner, and J. A. Theriot, "Mechanism of shape determination in motile cells," Nature 453, 475-480 (2008).
[CrossRef] [PubMed]

B. Bhaduri, A. Neild, and T. W. Ng, "Directional Brownian diffusion dynamics with variable magnitudes," Appl. Phys. Lett. 92, 084105 (2008).
[CrossRef]

D. J. Carnegie, D. J. Stevenson, M. Mazilu, F. Gunn-Moore, and K. Dholakia, "Guided neuronal growth using optical line traps," Opt. Express 16, 10507-10517 (2008).
[CrossRef] [PubMed]

2007

M. Poujade, E. Grasland-Mongrain, A. Hertzog, J. Jouanneau, P. Chavrier, B. Ladoux, A. Buguin, and P. Silberzan, "Collective migration of an epithelial monolayer in response to a model wound," Proc. Natl. Acad. Sci. USA 104, 15988-15993 (2007).
[CrossRef]

A. Bianco, M. Poukkula, A. Cliffe, J. Mathieu, C. M. Luque, T. A. Fulga, and P. Rørth, "Two distinct modes of guidance signaling during collective migration of border cells," Nature 448, 362-365 (2007).
[CrossRef] [PubMed]

D. R. Burnham, G. D. Wright, N. D. Read, and D. McGloin, "Holographic and single beam optical manipulation of hyphal growth in filamentous fungi," J. Opt. A: Pure Appl. Opt. 9, S172-S179 (2007).
[CrossRef]

R. Ananthakrishnan and A. Ehrlicher, "The forces behind cell movement," Int. J. Biol. Sci. 3, 303-317 (2007).
[PubMed]

2006

T. Betz, D. Lim, and J. A. Käs, "Neuronal Growth: a bistable stochastic process," Phys. Rev. Lett. 96, 098103 (2006).
[CrossRef] [PubMed]

2005

A. I. Norman, R. Ivkov, J. G. Forbes, and S. C. Greer, "The polymerization of actin: structural changes from small-angle neutron scattering," J. Chem. Phys. 123, 154904 (2005).
[CrossRef] [PubMed]

G. Garab, P. Galajda, I. Pomozi, L. Finzi, T. Praznovszky, P. Ormos, and H. van Amerongen, "Alignment of biological microparticles by a polarized laser beam," Eur. Biophys. J. 34, 335-343 (2005).
[CrossRef] [PubMed]

2004

A. La Porta and M. D. Wang, "Optical torque wrench: angular trapping, rotation, and torque detection of quartz microparticles," Phys. Rev. Lett. 92, 190801 (2004).
[CrossRef] [PubMed]

J. A. Dharmadhikari, S. Roy, A. K. Dharmadhikari, S. Sharma, and D. Mathur, "Torque-generating malaria-infected red blood cells in optical trap," Opt. Express 12, 1179-1184 (2004).
[CrossRef] [PubMed]

2003

S. Bayoudh, T. A. Nieminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, "Orientation of biological cells using plane polarization Gaussian beam optical tweezers," J. Mod. Opt. 50, 1581-1590 (2003).

A. I. Bishop, T. A. Nieminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, "Optical applcation and measurement of torque on microparticles of isotropic nonabsorbing material," Phys. Rev. A 68, 33802 (2003).
[CrossRef]

2002

A. Ehrlicher, T. Betz, B. Stuhrmann, D. Koch, V. Milner, M. G. Raizen, and J. A. Käs, "Guiding neuronal growth with light," Proc. Natl. Acad. Sci. 99, 16024-16028 (2002).
[CrossRef] [PubMed]

K. D. Bonin, B. Kourmanov, and T. G. Walker, "Light torque nanocontrol, nanomotors and nanorockers," Opt. Express 10, 984-989 (2002).
[PubMed]

2000

D. J. Odde and M. J. Renn, "Laser-guided direct writing of living cells," Biotechnol. Bioeng. 67, 312-318 (2000).
[CrossRef] [PubMed]

1998

M. E. Friese, T. A. Nieminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, "Optical alignment and spinning of laser-trapped microscopic particles," Nature 394, 348-350 (1998).
[CrossRef]

1994

K. Svoboda and S. M. Block, "Biological Applications of optical forces," Annu. Rev. Biophys. Biomol. Struct. 23, 247-285 (1994).
[CrossRef] [PubMed]

1987

A. Ashkin and J. M. Dziedzik, "Optical trapping and manipulation of viruses and bacteria," Science 235, 1517-1520 (1987).
[CrossRef] [PubMed]

1970

A. Ashkin, "Acceleration and trapping of particles by radiation pressure," Phys. Rev. Lett. 24, 156-159 (1970).
[CrossRef]

1936

R. A. Beth, "Mechanical detection and measurement of the angular momentum of light," Phys. Rev. 50, 115-125 (1936).
[CrossRef]

Albaladejo, S.

S. Albaladejo, M. I. Marqués, M. Laroche, and J. J. Sáenz, "Scattering forces from the curl of the spin angular momentum of a light field," Phys. Rev. Lett. 102, 113602 (2009).
[CrossRef] [PubMed]

Allen, G. M.

K. Keren, Z. Pincus, G. M. Allen, E. L. Barnhart, G. Marriott, A. Mogilner, and J. A. Theriot, "Mechanism of shape determination in motile cells," Nature 453, 475-480 (2008).
[CrossRef] [PubMed]

Amato-Grill, J.

Y. Roichman, B. Sun, Y. Roichman, J. Amato-Grill, and D. G. Grier, "Optical forces arising from phase gradients," Phys. Rev. Lett. 100, 013602 (2008).
[CrossRef] [PubMed]

Ananthakrishnan, R.

R. Ananthakrishnan and A. Ehrlicher, "The forces behind cell movement," Int. J. Biol. Sci. 3, 303-317 (2007).
[PubMed]

Ashkin, A.

A. Ashkin and J. M. Dziedzik, "Optical trapping and manipulation of viruses and bacteria," Science 235, 1517-1520 (1987).
[CrossRef] [PubMed]

A. Ashkin, "Acceleration and trapping of particles by radiation pressure," Phys. Rev. Lett. 24, 156-159 (1970).
[CrossRef]

Barnhart, E. L.

K. Keren, Z. Pincus, G. M. Allen, E. L. Barnhart, G. Marriott, A. Mogilner, and J. A. Theriot, "Mechanism of shape determination in motile cells," Nature 453, 475-480 (2008).
[CrossRef] [PubMed]

Bayoudh, S.

S. Bayoudh, T. A. Nieminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, "Orientation of biological cells using plane polarization Gaussian beam optical tweezers," J. Mod. Opt. 50, 1581-1590 (2003).

Beth, R. A.

R. A. Beth, "Mechanical detection and measurement of the angular momentum of light," Phys. Rev. 50, 115-125 (1936).
[CrossRef]

Betz, T.

T. Betz, D. Lim, and J. A. Käs, "Neuronal Growth: a bistable stochastic process," Phys. Rev. Lett. 96, 098103 (2006).
[CrossRef] [PubMed]

A. Ehrlicher, T. Betz, B. Stuhrmann, D. Koch, V. Milner, M. G. Raizen, and J. A. Käs, "Guiding neuronal growth with light," Proc. Natl. Acad. Sci. 99, 16024-16028 (2002).
[CrossRef] [PubMed]

Bhaduri, B.

B. Bhaduri, A. Neild, and T. W. Ng, "Directional Brownian diffusion dynamics with variable magnitudes," Appl. Phys. Lett. 92, 084105 (2008).
[CrossRef]

Bianco, A.

A. Bianco, M. Poukkula, A. Cliffe, J. Mathieu, C. M. Luque, T. A. Fulga, and P. Rørth, "Two distinct modes of guidance signaling during collective migration of border cells," Nature 448, 362-365 (2007).
[CrossRef] [PubMed]

Bishop, A. I.

A. I. Bishop, T. A. Nieminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, "Optical applcation and measurement of torque on microparticles of isotropic nonabsorbing material," Phys. Rev. A 68, 33802 (2003).
[CrossRef]

Block, S. M.

K. Svoboda and S. M. Block, "Biological Applications of optical forces," Annu. Rev. Biophys. Biomol. Struct. 23, 247-285 (1994).
[CrossRef] [PubMed]

Bonin, K. D.

Buguin, A.

M. Poujade, E. Grasland-Mongrain, A. Hertzog, J. Jouanneau, P. Chavrier, B. Ladoux, A. Buguin, and P. Silberzan, "Collective migration of an epithelial monolayer in response to a model wound," Proc. Natl. Acad. Sci. USA 104, 15988-15993 (2007).
[CrossRef]

Burnham, D. R.

D. R. Burnham, G. D. Wright, N. D. Read, and D. McGloin, "Holographic and single beam optical manipulation of hyphal growth in filamentous fungi," J. Opt. A: Pure Appl. Opt. 9, S172-S179 (2007).
[CrossRef]

Carnegie, D. J.

Chavrier, P.

M. Poujade, E. Grasland-Mongrain, A. Hertzog, J. Jouanneau, P. Chavrier, B. Ladoux, A. Buguin, and P. Silberzan, "Collective migration of an epithelial monolayer in response to a model wound," Proc. Natl. Acad. Sci. USA 104, 15988-15993 (2007).
[CrossRef]

Cliffe, A.

A. Bianco, M. Poukkula, A. Cliffe, J. Mathieu, C. M. Luque, T. A. Fulga, and P. Rørth, "Two distinct modes of guidance signaling during collective migration of border cells," Nature 448, 362-365 (2007).
[CrossRef] [PubMed]

Dharmadhikari, A. K.

Dharmadhikari, J. A.

Dholakia, K.

Dziedzik, J. M.

A. Ashkin and J. M. Dziedzik, "Optical trapping and manipulation of viruses and bacteria," Science 235, 1517-1520 (1987).
[CrossRef] [PubMed]

Ehrlicher, A.

R. Ananthakrishnan and A. Ehrlicher, "The forces behind cell movement," Int. J. Biol. Sci. 3, 303-317 (2007).
[PubMed]

A. Ehrlicher, T. Betz, B. Stuhrmann, D. Koch, V. Milner, M. G. Raizen, and J. A. Käs, "Guiding neuronal growth with light," Proc. Natl. Acad. Sci. 99, 16024-16028 (2002).
[CrossRef] [PubMed]

Finzi, L.

G. Garab, P. Galajda, I. Pomozi, L. Finzi, T. Praznovszky, P. Ormos, and H. van Amerongen, "Alignment of biological microparticles by a polarized laser beam," Eur. Biophys. J. 34, 335-343 (2005).
[CrossRef] [PubMed]

Forbes, J. G.

A. I. Norman, R. Ivkov, J. G. Forbes, and S. C. Greer, "The polymerization of actin: structural changes from small-angle neutron scattering," J. Chem. Phys. 123, 154904 (2005).
[CrossRef] [PubMed]

Friese, M. E.

M. E. Friese, T. A. Nieminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, "Optical alignment and spinning of laser-trapped microscopic particles," Nature 394, 348-350 (1998).
[CrossRef]

Fulga, T. A.

A. Bianco, M. Poukkula, A. Cliffe, J. Mathieu, C. M. Luque, T. A. Fulga, and P. Rørth, "Two distinct modes of guidance signaling during collective migration of border cells," Nature 448, 362-365 (2007).
[CrossRef] [PubMed]

Galajda, P.

G. Garab, P. Galajda, I. Pomozi, L. Finzi, T. Praznovszky, P. Ormos, and H. van Amerongen, "Alignment of biological microparticles by a polarized laser beam," Eur. Biophys. J. 34, 335-343 (2005).
[CrossRef] [PubMed]

Garab, G.

G. Garab, P. Galajda, I. Pomozi, L. Finzi, T. Praznovszky, P. Ormos, and H. van Amerongen, "Alignment of biological microparticles by a polarized laser beam," Eur. Biophys. J. 34, 335-343 (2005).
[CrossRef] [PubMed]

Grasland-Mongrain, E.

M. Poujade, E. Grasland-Mongrain, A. Hertzog, J. Jouanneau, P. Chavrier, B. Ladoux, A. Buguin, and P. Silberzan, "Collective migration of an epithelial monolayer in response to a model wound," Proc. Natl. Acad. Sci. USA 104, 15988-15993 (2007).
[CrossRef]

Greer, S. C.

A. I. Norman, R. Ivkov, J. G. Forbes, and S. C. Greer, "The polymerization of actin: structural changes from small-angle neutron scattering," J. Chem. Phys. 123, 154904 (2005).
[CrossRef] [PubMed]

Grier, D. G.

Y. Roichman, B. Sun, Y. Roichman, J. Amato-Grill, and D. G. Grier, "Optical forces arising from phase gradients," Phys. Rev. Lett. 100, 013602 (2008).
[CrossRef] [PubMed]

Gunn-Moore, F.

Heckenberg, N. R.

A. I. Bishop, T. A. Nieminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, "Optical applcation and measurement of torque on microparticles of isotropic nonabsorbing material," Phys. Rev. A 68, 33802 (2003).
[CrossRef]

S. Bayoudh, T. A. Nieminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, "Orientation of biological cells using plane polarization Gaussian beam optical tweezers," J. Mod. Opt. 50, 1581-1590 (2003).

M. E. Friese, T. A. Nieminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, "Optical alignment and spinning of laser-trapped microscopic particles," Nature 394, 348-350 (1998).
[CrossRef]

Hertzog, A.

M. Poujade, E. Grasland-Mongrain, A. Hertzog, J. Jouanneau, P. Chavrier, B. Ladoux, A. Buguin, and P. Silberzan, "Collective migration of an epithelial monolayer in response to a model wound," Proc. Natl. Acad. Sci. USA 104, 15988-15993 (2007).
[CrossRef]

Ivkov, R.

A. I. Norman, R. Ivkov, J. G. Forbes, and S. C. Greer, "The polymerization of actin: structural changes from small-angle neutron scattering," J. Chem. Phys. 123, 154904 (2005).
[CrossRef] [PubMed]

Jouanneau, J.

M. Poujade, E. Grasland-Mongrain, A. Hertzog, J. Jouanneau, P. Chavrier, B. Ladoux, A. Buguin, and P. Silberzan, "Collective migration of an epithelial monolayer in response to a model wound," Proc. Natl. Acad. Sci. USA 104, 15988-15993 (2007).
[CrossRef]

Käs, J. A.

T. Betz, D. Lim, and J. A. Käs, "Neuronal Growth: a bistable stochastic process," Phys. Rev. Lett. 96, 098103 (2006).
[CrossRef] [PubMed]

A. Ehrlicher, T. Betz, B. Stuhrmann, D. Koch, V. Milner, M. G. Raizen, and J. A. Käs, "Guiding neuronal growth with light," Proc. Natl. Acad. Sci. 99, 16024-16028 (2002).
[CrossRef] [PubMed]

Keren, K.

K. Keren, Z. Pincus, G. M. Allen, E. L. Barnhart, G. Marriott, A. Mogilner, and J. A. Theriot, "Mechanism of shape determination in motile cells," Nature 453, 475-480 (2008).
[CrossRef] [PubMed]

Koch, D.

A. Ehrlicher, T. Betz, B. Stuhrmann, D. Koch, V. Milner, M. G. Raizen, and J. A. Käs, "Guiding neuronal growth with light," Proc. Natl. Acad. Sci. 99, 16024-16028 (2002).
[CrossRef] [PubMed]

Kourmanov, B.

La Porta, A.

A. La Porta and M. D. Wang, "Optical torque wrench: angular trapping, rotation, and torque detection of quartz microparticles," Phys. Rev. Lett. 92, 190801 (2004).
[CrossRef] [PubMed]

Ladoux, B.

M. Poujade, E. Grasland-Mongrain, A. Hertzog, J. Jouanneau, P. Chavrier, B. Ladoux, A. Buguin, and P. Silberzan, "Collective migration of an epithelial monolayer in response to a model wound," Proc. Natl. Acad. Sci. USA 104, 15988-15993 (2007).
[CrossRef]

Laroche, M.

S. Albaladejo, M. I. Marqués, M. Laroche, and J. J. Sáenz, "Scattering forces from the curl of the spin angular momentum of a light field," Phys. Rev. Lett. 102, 113602 (2009).
[CrossRef] [PubMed]

Lim, D.

T. Betz, D. Lim, and J. A. Käs, "Neuronal Growth: a bistable stochastic process," Phys. Rev. Lett. 96, 098103 (2006).
[CrossRef] [PubMed]

Luque, C. M.

A. Bianco, M. Poukkula, A. Cliffe, J. Mathieu, C. M. Luque, T. A. Fulga, and P. Rørth, "Two distinct modes of guidance signaling during collective migration of border cells," Nature 448, 362-365 (2007).
[CrossRef] [PubMed]

Marqués, M. I.

S. Albaladejo, M. I. Marqués, M. Laroche, and J. J. Sáenz, "Scattering forces from the curl of the spin angular momentum of a light field," Phys. Rev. Lett. 102, 113602 (2009).
[CrossRef] [PubMed]

Marriott, G.

K. Keren, Z. Pincus, G. M. Allen, E. L. Barnhart, G. Marriott, A. Mogilner, and J. A. Theriot, "Mechanism of shape determination in motile cells," Nature 453, 475-480 (2008).
[CrossRef] [PubMed]

Mathieu, J.

A. Bianco, M. Poukkula, A. Cliffe, J. Mathieu, C. M. Luque, T. A. Fulga, and P. Rørth, "Two distinct modes of guidance signaling during collective migration of border cells," Nature 448, 362-365 (2007).
[CrossRef] [PubMed]

Mathur, D.

Mazilu, M.

McGloin, D.

D. R. Burnham, G. D. Wright, N. D. Read, and D. McGloin, "Holographic and single beam optical manipulation of hyphal growth in filamentous fungi," J. Opt. A: Pure Appl. Opt. 9, S172-S179 (2007).
[CrossRef]

Milner, V.

A. Ehrlicher, T. Betz, B. Stuhrmann, D. Koch, V. Milner, M. G. Raizen, and J. A. Käs, "Guiding neuronal growth with light," Proc. Natl. Acad. Sci. 99, 16024-16028 (2002).
[CrossRef] [PubMed]

Mogilner, A.

K. Keren, Z. Pincus, G. M. Allen, E. L. Barnhart, G. Marriott, A. Mogilner, and J. A. Theriot, "Mechanism of shape determination in motile cells," Nature 453, 475-480 (2008).
[CrossRef] [PubMed]

Neild, A.

B. Bhaduri, A. Neild, and T. W. Ng, "Directional Brownian diffusion dynamics with variable magnitudes," Appl. Phys. Lett. 92, 084105 (2008).
[CrossRef]

Ng, T. W.

B. Bhaduri, A. Neild, and T. W. Ng, "Directional Brownian diffusion dynamics with variable magnitudes," Appl. Phys. Lett. 92, 084105 (2008).
[CrossRef]

Nieminen, T. A.

A. I. Bishop, T. A. Nieminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, "Optical applcation and measurement of torque on microparticles of isotropic nonabsorbing material," Phys. Rev. A 68, 33802 (2003).
[CrossRef]

S. Bayoudh, T. A. Nieminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, "Orientation of biological cells using plane polarization Gaussian beam optical tweezers," J. Mod. Opt. 50, 1581-1590 (2003).

M. E. Friese, T. A. Nieminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, "Optical alignment and spinning of laser-trapped microscopic particles," Nature 394, 348-350 (1998).
[CrossRef]

Norman, A. I.

A. I. Norman, R. Ivkov, J. G. Forbes, and S. C. Greer, "The polymerization of actin: structural changes from small-angle neutron scattering," J. Chem. Phys. 123, 154904 (2005).
[CrossRef] [PubMed]

Odde, D. J.

D. J. Odde and M. J. Renn, "Laser-guided direct writing of living cells," Biotechnol. Bioeng. 67, 312-318 (2000).
[CrossRef] [PubMed]

Ormos, P.

G. Garab, P. Galajda, I. Pomozi, L. Finzi, T. Praznovszky, P. Ormos, and H. van Amerongen, "Alignment of biological microparticles by a polarized laser beam," Eur. Biophys. J. 34, 335-343 (2005).
[CrossRef] [PubMed]

Pincus, Z.

K. Keren, Z. Pincus, G. M. Allen, E. L. Barnhart, G. Marriott, A. Mogilner, and J. A. Theriot, "Mechanism of shape determination in motile cells," Nature 453, 475-480 (2008).
[CrossRef] [PubMed]

Pomozi, I.

G. Garab, P. Galajda, I. Pomozi, L. Finzi, T. Praznovszky, P. Ormos, and H. van Amerongen, "Alignment of biological microparticles by a polarized laser beam," Eur. Biophys. J. 34, 335-343 (2005).
[CrossRef] [PubMed]

Poujade, M.

M. Poujade, E. Grasland-Mongrain, A. Hertzog, J. Jouanneau, P. Chavrier, B. Ladoux, A. Buguin, and P. Silberzan, "Collective migration of an epithelial monolayer in response to a model wound," Proc. Natl. Acad. Sci. USA 104, 15988-15993 (2007).
[CrossRef]

Poukkula, M.

A. Bianco, M. Poukkula, A. Cliffe, J. Mathieu, C. M. Luque, T. A. Fulga, and P. Rørth, "Two distinct modes of guidance signaling during collective migration of border cells," Nature 448, 362-365 (2007).
[CrossRef] [PubMed]

Praznovszky, T.

G. Garab, P. Galajda, I. Pomozi, L. Finzi, T. Praznovszky, P. Ormos, and H. van Amerongen, "Alignment of biological microparticles by a polarized laser beam," Eur. Biophys. J. 34, 335-343 (2005).
[CrossRef] [PubMed]

Raizen, M. G.

A. Ehrlicher, T. Betz, B. Stuhrmann, D. Koch, V. Milner, M. G. Raizen, and J. A. Käs, "Guiding neuronal growth with light," Proc. Natl. Acad. Sci. 99, 16024-16028 (2002).
[CrossRef] [PubMed]

Read, N. D.

D. R. Burnham, G. D. Wright, N. D. Read, and D. McGloin, "Holographic and single beam optical manipulation of hyphal growth in filamentous fungi," J. Opt. A: Pure Appl. Opt. 9, S172-S179 (2007).
[CrossRef]

Renn, M. J.

D. J. Odde and M. J. Renn, "Laser-guided direct writing of living cells," Biotechnol. Bioeng. 67, 312-318 (2000).
[CrossRef] [PubMed]

Roichman, Y.

Y. Roichman, B. Sun, Y. Roichman, J. Amato-Grill, and D. G. Grier, "Optical forces arising from phase gradients," Phys. Rev. Lett. 100, 013602 (2008).
[CrossRef] [PubMed]

Y. Roichman, B. Sun, Y. Roichman, J. Amato-Grill, and D. G. Grier, "Optical forces arising from phase gradients," Phys. Rev. Lett. 100, 013602 (2008).
[CrossRef] [PubMed]

Rørth, P.

A. Bianco, M. Poukkula, A. Cliffe, J. Mathieu, C. M. Luque, T. A. Fulga, and P. Rørth, "Two distinct modes of guidance signaling during collective migration of border cells," Nature 448, 362-365 (2007).
[CrossRef] [PubMed]

Roy, S.

Rubinsztein-Dunlop, H.

A. I. Bishop, T. A. Nieminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, "Optical applcation and measurement of torque on microparticles of isotropic nonabsorbing material," Phys. Rev. A 68, 33802 (2003).
[CrossRef]

S. Bayoudh, T. A. Nieminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, "Orientation of biological cells using plane polarization Gaussian beam optical tweezers," J. Mod. Opt. 50, 1581-1590 (2003).

M. E. Friese, T. A. Nieminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, "Optical alignment and spinning of laser-trapped microscopic particles," Nature 394, 348-350 (1998).
[CrossRef]

Sáenz, J. J.

S. Albaladejo, M. I. Marqués, M. Laroche, and J. J. Sáenz, "Scattering forces from the curl of the spin angular momentum of a light field," Phys. Rev. Lett. 102, 113602 (2009).
[CrossRef] [PubMed]

Sharma, S.

Silberzan, P.

M. Poujade, E. Grasland-Mongrain, A. Hertzog, J. Jouanneau, P. Chavrier, B. Ladoux, A. Buguin, and P. Silberzan, "Collective migration of an epithelial monolayer in response to a model wound," Proc. Natl. Acad. Sci. USA 104, 15988-15993 (2007).
[CrossRef]

Stevenson, D. J.

Stuhrmann, B.

A. Ehrlicher, T. Betz, B. Stuhrmann, D. Koch, V. Milner, M. G. Raizen, and J. A. Käs, "Guiding neuronal growth with light," Proc. Natl. Acad. Sci. 99, 16024-16028 (2002).
[CrossRef] [PubMed]

Sun, B.

Y. Roichman, B. Sun, Y. Roichman, J. Amato-Grill, and D. G. Grier, "Optical forces arising from phase gradients," Phys. Rev. Lett. 100, 013602 (2008).
[CrossRef] [PubMed]

Svoboda, K.

K. Svoboda and S. M. Block, "Biological Applications of optical forces," Annu. Rev. Biophys. Biomol. Struct. 23, 247-285 (1994).
[CrossRef] [PubMed]

Theriot, J. A.

K. Keren, Z. Pincus, G. M. Allen, E. L. Barnhart, G. Marriott, A. Mogilner, and J. A. Theriot, "Mechanism of shape determination in motile cells," Nature 453, 475-480 (2008).
[CrossRef] [PubMed]

van Amerongen, H.

G. Garab, P. Galajda, I. Pomozi, L. Finzi, T. Praznovszky, P. Ormos, and H. van Amerongen, "Alignment of biological microparticles by a polarized laser beam," Eur. Biophys. J. 34, 335-343 (2005).
[CrossRef] [PubMed]

Walker, T. G.

Wang, M. D.

A. La Porta and M. D. Wang, "Optical torque wrench: angular trapping, rotation, and torque detection of quartz microparticles," Phys. Rev. Lett. 92, 190801 (2004).
[CrossRef] [PubMed]

Wright, G. D.

D. R. Burnham, G. D. Wright, N. D. Read, and D. McGloin, "Holographic and single beam optical manipulation of hyphal growth in filamentous fungi," J. Opt. A: Pure Appl. Opt. 9, S172-S179 (2007).
[CrossRef]

Annu. Rev. Biophys. Biomol. Struct.

K. Svoboda and S. M. Block, "Biological Applications of optical forces," Annu. Rev. Biophys. Biomol. Struct. 23, 247-285 (1994).
[CrossRef] [PubMed]

Appl. Phys. Lett.

B. Bhaduri, A. Neild, and T. W. Ng, "Directional Brownian diffusion dynamics with variable magnitudes," Appl. Phys. Lett. 92, 084105 (2008).
[CrossRef]

Biotechnol. Bioeng.

D. J. Odde and M. J. Renn, "Laser-guided direct writing of living cells," Biotechnol. Bioeng. 67, 312-318 (2000).
[CrossRef] [PubMed]

Eur. Biophys. J.

G. Garab, P. Galajda, I. Pomozi, L. Finzi, T. Praznovszky, P. Ormos, and H. van Amerongen, "Alignment of biological microparticles by a polarized laser beam," Eur. Biophys. J. 34, 335-343 (2005).
[CrossRef] [PubMed]

Int. J. Biol. Sci.

R. Ananthakrishnan and A. Ehrlicher, "The forces behind cell movement," Int. J. Biol. Sci. 3, 303-317 (2007).
[PubMed]

J. Chem. Phys.

A. I. Norman, R. Ivkov, J. G. Forbes, and S. C. Greer, "The polymerization of actin: structural changes from small-angle neutron scattering," J. Chem. Phys. 123, 154904 (2005).
[CrossRef] [PubMed]

J. Mod. Opt.

S. Bayoudh, T. A. Nieminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, "Orientation of biological cells using plane polarization Gaussian beam optical tweezers," J. Mod. Opt. 50, 1581-1590 (2003).

J. Opt. A: Pure Appl. Opt.

D. R. Burnham, G. D. Wright, N. D. Read, and D. McGloin, "Holographic and single beam optical manipulation of hyphal growth in filamentous fungi," J. Opt. A: Pure Appl. Opt. 9, S172-S179 (2007).
[CrossRef]

Nature

K. Keren, Z. Pincus, G. M. Allen, E. L. Barnhart, G. Marriott, A. Mogilner, and J. A. Theriot, "Mechanism of shape determination in motile cells," Nature 453, 475-480 (2008).
[CrossRef] [PubMed]

M. E. Friese, T. A. Nieminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, "Optical alignment and spinning of laser-trapped microscopic particles," Nature 394, 348-350 (1998).
[CrossRef]

A. Bianco, M. Poukkula, A. Cliffe, J. Mathieu, C. M. Luque, T. A. Fulga, and P. Rørth, "Two distinct modes of guidance signaling during collective migration of border cells," Nature 448, 362-365 (2007).
[CrossRef] [PubMed]

Opt. Express

Phys. Rev.

R. A. Beth, "Mechanical detection and measurement of the angular momentum of light," Phys. Rev. 50, 115-125 (1936).
[CrossRef]

Phys. Rev. A

A. I. Bishop, T. A. Nieminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, "Optical applcation and measurement of torque on microparticles of isotropic nonabsorbing material," Phys. Rev. A 68, 33802 (2003).
[CrossRef]

Phys. Rev. Lett.

A. La Porta and M. D. Wang, "Optical torque wrench: angular trapping, rotation, and torque detection of quartz microparticles," Phys. Rev. Lett. 92, 190801 (2004).
[CrossRef] [PubMed]

Y. Roichman, B. Sun, Y. Roichman, J. Amato-Grill, and D. G. Grier, "Optical forces arising from phase gradients," Phys. Rev. Lett. 100, 013602 (2008).
[CrossRef] [PubMed]

S. Albaladejo, M. I. Marqués, M. Laroche, and J. J. Sáenz, "Scattering forces from the curl of the spin angular momentum of a light field," Phys. Rev. Lett. 102, 113602 (2009).
[CrossRef] [PubMed]

A. Ashkin, "Acceleration and trapping of particles by radiation pressure," Phys. Rev. Lett. 24, 156-159 (1970).
[CrossRef]

T. Betz, D. Lim, and J. A. Käs, "Neuronal Growth: a bistable stochastic process," Phys. Rev. Lett. 96, 098103 (2006).
[CrossRef] [PubMed]

Proc. Natl. Acad. Sci.

A. Ehrlicher, T. Betz, B. Stuhrmann, D. Koch, V. Milner, M. G. Raizen, and J. A. Käs, "Guiding neuronal growth with light," Proc. Natl. Acad. Sci. 99, 16024-16028 (2002).
[CrossRef] [PubMed]

Proc. Natl. Acad. Sci. USA

M. Poujade, E. Grasland-Mongrain, A. Hertzog, J. Jouanneau, P. Chavrier, B. Ladoux, A. Buguin, and P. Silberzan, "Collective migration of an epithelial monolayer in response to a model wound," Proc. Natl. Acad. Sci. USA 104, 15988-15993 (2007).
[CrossRef]

Science

A. Ashkin and J. M. Dziedzik, "Optical trapping and manipulation of viruses and bacteria," Science 235, 1517-1520 (1987).
[CrossRef] [PubMed]

Other

H. Lodish, A. Berk, S. L. Zipursky, P. Matsudaira, D. Baltimore, and J. E. Darnell, Molecular Cell Biology (W. H. Freeman, New York, 2004).

L. Allen, S. M. Barnett, M. J. Padgett, Optical Angular Momentum (Institute of Physics Pub., Bristol, 2003).
[CrossRef]

Supplementary Material (3)

» Media 1: MOV (4063 KB)     
» Media 2: MOV (1582 KB)     
» Media 3: MOV (805 KB)     

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (5)

Fig. 1.
Fig. 1.

(a-c) Results of numerical simulations illustrating the orientation of 1×10µm rods before and after 2 and, respectively, 4.5 seconds after the irradiation with light polarized along the indicated direction. (d-f) The temporal evolution of the angular orientation of the same rods; the rods in (e) and (f) were subjected to five and, respectively, ten times more intense radiation then those in (d). (g-i) The temporal evolution of the angular orientation of rods of different sizes: (g) 0.2×2 µm, (h) 0.5×5 µm, and (i) 1×10 µm.

Fig. 2.
Fig. 2.

The experimental setup comprises a laser beam passing a polarization state generator (PSG) and illuminating a cell culture through a microscope objective (MO). The cells are placed inside an incubation chamber (IC) and are imaged using an unpolarized light source and a charged coupled device (CCD).

Fig. 3.
Fig. 3.

(a) Fluorescence image of fixed SH-SY5 cells that were not subjected to illumination. (b) Image of fixed SH-Sy5 cells after being illuminated with light linearly polarized at 45 as indicated. (c, d) Fourier transform distributions of the corresponding cell images in (a) and (b).

Fig. 4.
Fig. 4.

(a) The time evolution of an ensemble of SH-Sy5 cells exposed to unpolarized light. (Media 1) (b) and (c) Two different examples of the time evolution of SH-Sy5 cells exposed to light linearly polarized along the direction indicated by the arrows. In time, an evident structure formation develops along the direction of polarization as explained in the text (Media 2 and Media 3).

Fig. 5.
Fig. 5.

(a), (b) ensembles of cells illuminated with light linearly polarized horizontally and at 45° as indicated. (c), (d) Low spatial frequency region of the corresponding two-dimensional Fourier transforms of the images in (a) and (b). (e), (f) Cross sections of the two Fourier transforms along the directions indicated in (c) and (d).

Equations (8)

Equations on this page are rendered with MathJax. Learn more.

2 x t 2 = Γ 1 ( t ) x t + ξ ( t ) + F ( t )
2 θ t 2 = Γ θ 1 ( t ) θ t + ξ θ ( t ) + T ( t )
x t = ξ ( t ) + Γ ( t ) F ( t ) ,
θ t = ξ θ ( t ) + Γ θ T ( t ) ,
ξ ( t ) ξ ( t ) = 2 k B T Γ ( t ) δ ( t t ) = 2 D ( t ) δ ( t t )
D a = k B T [ l n ( 2 r ) 0 . 5 ] 2 π η s L , D b = k B T [ ln ( 2 r ) + 0 . 5 ] 4 π η s L , D θ = 3 k B T [ ln ( 2 r ) 0 . 5 ] π η s L 3 .
T = d 3 x P × E = I 0 ( χ o χ e ) A t cos ( 2 θ ) ,
θ = ζ ( t ) + tan 1 [ tan ( θ 0 ) e α t ] ,

Metrics