Abstract

The aggregation of a β-crystallin water solution exposed to XeCl laser radiation demonstrates the dependence of scattering-exposure curve (scattering versus exposure) on laser intensity. The main features of this dependence can be understood by the relaxation of a partly denaturated state of a protein within some finite relaxation time. These photoactivated states originate from the absorption of UV photons. Two partly denaturated (photoactivated) monomers, as well as other aggregates, can aggregate, giving rise to sharply increasing probe light scattering after some lag time of irradiation.

© 2008 Optical Society of America

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  1. V. V. El'chaninov and I. B. Fedorovich, "Mechanisms of photodestruction of the eye structure. Formation of polypeptide aggregates upon UV-irradiation of lens proteins," Biofizika 34, 758-762 (1989).
    [PubMed]
  2. M. A. Ostrovsky, I. B. Fedorovich, V. V. El'chaninov, and A. V. Krivandin, "Danger of damaging effect of light on the eye structures: Eye lens like a natural light filter and object of photodestruction," Sensor. Sistemy 8, 135-146 (1994).
  3. J.-H. Liao, J.-S. Lee, and S.-H. Chiou, "Distinct roles of ?A- and ?B-crystallins under thermal and UV stresses," Biochem. Biophys. Res. Commun. 295, 854-861 (2002). [Cross Ref]
    [CrossRef] [PubMed]
  4. A. V. Krivandin, Yu. M. L'vov, M. A. Ostrovsky, I. B. Fedorovich, and L. A. Feigin, "Structural conversions of crystallins under senile cataract, dehydration and UV-irradiation studied by X-ray diffraction," Exp. Eye Res. 49, 853-859 (1989). [Cross Ref]
    [CrossRef] [PubMed]
  5. R. F. Borkman, G. Knight, and B. Obi, "The Molecular Chaperone ?-Crystallin Inhibits UV-Induced Protein Aggregation," Exp. Eye. Res. 62, 141-148 (1996). [Cross Ref]
    [CrossRef] [PubMed]
  6. R. F. Borkman and J. McLaughlin, "The molecular chaperone function of ?-crystallin is impaired by UV photolysis," Photochem. Photobiol. 62, 1046-1051 (1995). [Cross Ref]
    [CrossRef] [PubMed]
  7. Yu. V. Sergeev, L. V. Soustov, E. V. Chelnokov, N. M. Bityurin, P. S. BacklundJr., P. T. Wingfield, M. A. Ostrovsky, and J. Fielding Hejtmancik, "Increased Sensitivity of Amino-Arm Truncated ßA3-Crystallin to UV-Light-Induced Photoaggregation," Invest Ophthalmol. Visual Sci. 46, 3263-3273 (2005). [Cross Ref]
    [CrossRef]
  8. M. Rothschild and D. J. Ehrlich, "A review of excimer laser projection lithography," J. Vac. Sci. Technol. B 6, 1-17 (1988). [Cross Ref]
    [CrossRef]
  9. L. V. Soustov, E. V. Chelnokov, N. M. Bityurin, V. V. Nemov, Yu. V. Sergeev, and M. A. Ostrovsky, "Photoaggregation of Water-soluble Protein (Carboanhydrase) Induced by the Ultraviolet Radiation of Xe-Cl Laser," Dokl. Biochem. Biophys. 388, 49-54 (2003).
    [CrossRef] [PubMed]
  10. E. L. Bubis, V. V. Var'gin, L. R. Konchalina, and A. A. Shilov, "Study of low-absorption media for SBS in the near-IR spectral range," Opt. Spektrosk. 65, 1281-1285 (1988).
  11. M. M. Korkhmazyan, I. B. Fedorovich, and M. A. Ostrovsky, "Mechanisms of photodamage of eye structures. The effect of UV light on soluble lens proteins," Biofizika 28, 966-971 (1983).
  12. N. M. Bityurin, S. V. Muraviov, V. A. Kamensky, A. Y. Malyshev, E. V. Chelnokov, L. V. Soustov, and G. V. Gelikonov, "Kinetics of low-scattering biotissue photodenaturation induced by the UV harmonics of a Nd:YAP laser and by Nd:YAG laser at a wavelength of 1440 nm," Proc. SPIE 4161, 1-11 (2000).
    [CrossRef]
  13. N. L. Vekshin and V. I. Sukharev, "Thermo- and photoinduced aggregation of alpha-crystallin," Biofizika 50, 236-242 (2005).
    [PubMed]

2005 (2)

Yu. V. Sergeev, L. V. Soustov, E. V. Chelnokov, N. M. Bityurin, P. S. BacklundJr., P. T. Wingfield, M. A. Ostrovsky, and J. Fielding Hejtmancik, "Increased Sensitivity of Amino-Arm Truncated ßA3-Crystallin to UV-Light-Induced Photoaggregation," Invest Ophthalmol. Visual Sci. 46, 3263-3273 (2005). [Cross Ref]
[CrossRef]

N. L. Vekshin and V. I. Sukharev, "Thermo- and photoinduced aggregation of alpha-crystallin," Biofizika 50, 236-242 (2005).
[PubMed]

2003 (1)

L. V. Soustov, E. V. Chelnokov, N. M. Bityurin, V. V. Nemov, Yu. V. Sergeev, and M. A. Ostrovsky, "Photoaggregation of Water-soluble Protein (Carboanhydrase) Induced by the Ultraviolet Radiation of Xe-Cl Laser," Dokl. Biochem. Biophys. 388, 49-54 (2003).
[CrossRef] [PubMed]

2002 (1)

J.-H. Liao, J.-S. Lee, and S.-H. Chiou, "Distinct roles of ?A- and ?B-crystallins under thermal and UV stresses," Biochem. Biophys. Res. Commun. 295, 854-861 (2002). [Cross Ref]
[CrossRef] [PubMed]

2000 (1)

N. M. Bityurin, S. V. Muraviov, V. A. Kamensky, A. Y. Malyshev, E. V. Chelnokov, L. V. Soustov, and G. V. Gelikonov, "Kinetics of low-scattering biotissue photodenaturation induced by the UV harmonics of a Nd:YAP laser and by Nd:YAG laser at a wavelength of 1440 nm," Proc. SPIE 4161, 1-11 (2000).
[CrossRef]

1996 (1)

R. F. Borkman, G. Knight, and B. Obi, "The Molecular Chaperone ?-Crystallin Inhibits UV-Induced Protein Aggregation," Exp. Eye. Res. 62, 141-148 (1996). [Cross Ref]
[CrossRef] [PubMed]

1995 (1)

R. F. Borkman and J. McLaughlin, "The molecular chaperone function of ?-crystallin is impaired by UV photolysis," Photochem. Photobiol. 62, 1046-1051 (1995). [Cross Ref]
[CrossRef] [PubMed]

1994 (1)

M. A. Ostrovsky, I. B. Fedorovich, V. V. El'chaninov, and A. V. Krivandin, "Danger of damaging effect of light on the eye structures: Eye lens like a natural light filter and object of photodestruction," Sensor. Sistemy 8, 135-146 (1994).

1989 (2)

V. V. El'chaninov and I. B. Fedorovich, "Mechanisms of photodestruction of the eye structure. Formation of polypeptide aggregates upon UV-irradiation of lens proteins," Biofizika 34, 758-762 (1989).
[PubMed]

A. V. Krivandin, Yu. M. L'vov, M. A. Ostrovsky, I. B. Fedorovich, and L. A. Feigin, "Structural conversions of crystallins under senile cataract, dehydration and UV-irradiation studied by X-ray diffraction," Exp. Eye Res. 49, 853-859 (1989). [Cross Ref]
[CrossRef] [PubMed]

1988 (2)

M. Rothschild and D. J. Ehrlich, "A review of excimer laser projection lithography," J. Vac. Sci. Technol. B 6, 1-17 (1988). [Cross Ref]
[CrossRef]

E. L. Bubis, V. V. Var'gin, L. R. Konchalina, and A. A. Shilov, "Study of low-absorption media for SBS in the near-IR spectral range," Opt. Spektrosk. 65, 1281-1285 (1988).

1983 (1)

M. M. Korkhmazyan, I. B. Fedorovich, and M. A. Ostrovsky, "Mechanisms of photodamage of eye structures. The effect of UV light on soluble lens proteins," Biofizika 28, 966-971 (1983).

Backlund, P. S.

Yu. V. Sergeev, L. V. Soustov, E. V. Chelnokov, N. M. Bityurin, P. S. BacklundJr., P. T. Wingfield, M. A. Ostrovsky, and J. Fielding Hejtmancik, "Increased Sensitivity of Amino-Arm Truncated ßA3-Crystallin to UV-Light-Induced Photoaggregation," Invest Ophthalmol. Visual Sci. 46, 3263-3273 (2005). [Cross Ref]
[CrossRef]

Bityurin, N. M.

Yu. V. Sergeev, L. V. Soustov, E. V. Chelnokov, N. M. Bityurin, P. S. BacklundJr., P. T. Wingfield, M. A. Ostrovsky, and J. Fielding Hejtmancik, "Increased Sensitivity of Amino-Arm Truncated ßA3-Crystallin to UV-Light-Induced Photoaggregation," Invest Ophthalmol. Visual Sci. 46, 3263-3273 (2005). [Cross Ref]
[CrossRef]

L. V. Soustov, E. V. Chelnokov, N. M. Bityurin, V. V. Nemov, Yu. V. Sergeev, and M. A. Ostrovsky, "Photoaggregation of Water-soluble Protein (Carboanhydrase) Induced by the Ultraviolet Radiation of Xe-Cl Laser," Dokl. Biochem. Biophys. 388, 49-54 (2003).
[CrossRef] [PubMed]

N. M. Bityurin, S. V. Muraviov, V. A. Kamensky, A. Y. Malyshev, E. V. Chelnokov, L. V. Soustov, and G. V. Gelikonov, "Kinetics of low-scattering biotissue photodenaturation induced by the UV harmonics of a Nd:YAP laser and by Nd:YAG laser at a wavelength of 1440 nm," Proc. SPIE 4161, 1-11 (2000).
[CrossRef]

Borkman, R. F.

R. F. Borkman, G. Knight, and B. Obi, "The Molecular Chaperone ?-Crystallin Inhibits UV-Induced Protein Aggregation," Exp. Eye. Res. 62, 141-148 (1996). [Cross Ref]
[CrossRef] [PubMed]

R. F. Borkman and J. McLaughlin, "The molecular chaperone function of ?-crystallin is impaired by UV photolysis," Photochem. Photobiol. 62, 1046-1051 (1995). [Cross Ref]
[CrossRef] [PubMed]

Bubis, E. L.

E. L. Bubis, V. V. Var'gin, L. R. Konchalina, and A. A. Shilov, "Study of low-absorption media for SBS in the near-IR spectral range," Opt. Spektrosk. 65, 1281-1285 (1988).

Chelnokov, E. V.

Yu. V. Sergeev, L. V. Soustov, E. V. Chelnokov, N. M. Bityurin, P. S. BacklundJr., P. T. Wingfield, M. A. Ostrovsky, and J. Fielding Hejtmancik, "Increased Sensitivity of Amino-Arm Truncated ßA3-Crystallin to UV-Light-Induced Photoaggregation," Invest Ophthalmol. Visual Sci. 46, 3263-3273 (2005). [Cross Ref]
[CrossRef]

L. V. Soustov, E. V. Chelnokov, N. M. Bityurin, V. V. Nemov, Yu. V. Sergeev, and M. A. Ostrovsky, "Photoaggregation of Water-soluble Protein (Carboanhydrase) Induced by the Ultraviolet Radiation of Xe-Cl Laser," Dokl. Biochem. Biophys. 388, 49-54 (2003).
[CrossRef] [PubMed]

N. M. Bityurin, S. V. Muraviov, V. A. Kamensky, A. Y. Malyshev, E. V. Chelnokov, L. V. Soustov, and G. V. Gelikonov, "Kinetics of low-scattering biotissue photodenaturation induced by the UV harmonics of a Nd:YAP laser and by Nd:YAG laser at a wavelength of 1440 nm," Proc. SPIE 4161, 1-11 (2000).
[CrossRef]

Chiou, S.-H.

J.-H. Liao, J.-S. Lee, and S.-H. Chiou, "Distinct roles of ?A- and ?B-crystallins under thermal and UV stresses," Biochem. Biophys. Res. Commun. 295, 854-861 (2002). [Cross Ref]
[CrossRef] [PubMed]

Ehrlich, D. J.

M. Rothschild and D. J. Ehrlich, "A review of excimer laser projection lithography," J. Vac. Sci. Technol. B 6, 1-17 (1988). [Cross Ref]
[CrossRef]

El'chaninov, V. V.

M. A. Ostrovsky, I. B. Fedorovich, V. V. El'chaninov, and A. V. Krivandin, "Danger of damaging effect of light on the eye structures: Eye lens like a natural light filter and object of photodestruction," Sensor. Sistemy 8, 135-146 (1994).

V. V. El'chaninov and I. B. Fedorovich, "Mechanisms of photodestruction of the eye structure. Formation of polypeptide aggregates upon UV-irradiation of lens proteins," Biofizika 34, 758-762 (1989).
[PubMed]

Fedorovich, I. B.

M. A. Ostrovsky, I. B. Fedorovich, V. V. El'chaninov, and A. V. Krivandin, "Danger of damaging effect of light on the eye structures: Eye lens like a natural light filter and object of photodestruction," Sensor. Sistemy 8, 135-146 (1994).

V. V. El'chaninov and I. B. Fedorovich, "Mechanisms of photodestruction of the eye structure. Formation of polypeptide aggregates upon UV-irradiation of lens proteins," Biofizika 34, 758-762 (1989).
[PubMed]

M. M. Korkhmazyan, I. B. Fedorovich, and M. A. Ostrovsky, "Mechanisms of photodamage of eye structures. The effect of UV light on soluble lens proteins," Biofizika 28, 966-971 (1983).

Fielding Hejtmancik, J.

Yu. V. Sergeev, L. V. Soustov, E. V. Chelnokov, N. M. Bityurin, P. S. BacklundJr., P. T. Wingfield, M. A. Ostrovsky, and J. Fielding Hejtmancik, "Increased Sensitivity of Amino-Arm Truncated ßA3-Crystallin to UV-Light-Induced Photoaggregation," Invest Ophthalmol. Visual Sci. 46, 3263-3273 (2005). [Cross Ref]
[CrossRef]

Gelikonov, G. V.

N. M. Bityurin, S. V. Muraviov, V. A. Kamensky, A. Y. Malyshev, E. V. Chelnokov, L. V. Soustov, and G. V. Gelikonov, "Kinetics of low-scattering biotissue photodenaturation induced by the UV harmonics of a Nd:YAP laser and by Nd:YAG laser at a wavelength of 1440 nm," Proc. SPIE 4161, 1-11 (2000).
[CrossRef]

Kamensky, V. A.

N. M. Bityurin, S. V. Muraviov, V. A. Kamensky, A. Y. Malyshev, E. V. Chelnokov, L. V. Soustov, and G. V. Gelikonov, "Kinetics of low-scattering biotissue photodenaturation induced by the UV harmonics of a Nd:YAP laser and by Nd:YAG laser at a wavelength of 1440 nm," Proc. SPIE 4161, 1-11 (2000).
[CrossRef]

Knight, G.

R. F. Borkman, G. Knight, and B. Obi, "The Molecular Chaperone ?-Crystallin Inhibits UV-Induced Protein Aggregation," Exp. Eye. Res. 62, 141-148 (1996). [Cross Ref]
[CrossRef] [PubMed]

Konchalina, L. R.

E. L. Bubis, V. V. Var'gin, L. R. Konchalina, and A. A. Shilov, "Study of low-absorption media for SBS in the near-IR spectral range," Opt. Spektrosk. 65, 1281-1285 (1988).

Korkhmazyan, M. M.

M. M. Korkhmazyan, I. B. Fedorovich, and M. A. Ostrovsky, "Mechanisms of photodamage of eye structures. The effect of UV light on soluble lens proteins," Biofizika 28, 966-971 (1983).

Krivandin, A. V.

M. A. Ostrovsky, I. B. Fedorovich, V. V. El'chaninov, and A. V. Krivandin, "Danger of damaging effect of light on the eye structures: Eye lens like a natural light filter and object of photodestruction," Sensor. Sistemy 8, 135-146 (1994).

A. V. Krivandin, Yu. M. L'vov, M. A. Ostrovsky, I. B. Fedorovich, and L. A. Feigin, "Structural conversions of crystallins under senile cataract, dehydration and UV-irradiation studied by X-ray diffraction," Exp. Eye Res. 49, 853-859 (1989). [Cross Ref]
[CrossRef] [PubMed]

Lee, J.-S.

J.-H. Liao, J.-S. Lee, and S.-H. Chiou, "Distinct roles of ?A- and ?B-crystallins under thermal and UV stresses," Biochem. Biophys. Res. Commun. 295, 854-861 (2002). [Cross Ref]
[CrossRef] [PubMed]

Liao, J.-H.

J.-H. Liao, J.-S. Lee, and S.-H. Chiou, "Distinct roles of ?A- and ?B-crystallins under thermal and UV stresses," Biochem. Biophys. Res. Commun. 295, 854-861 (2002). [Cross Ref]
[CrossRef] [PubMed]

Malyshev, A. Y.

N. M. Bityurin, S. V. Muraviov, V. A. Kamensky, A. Y. Malyshev, E. V. Chelnokov, L. V. Soustov, and G. V. Gelikonov, "Kinetics of low-scattering biotissue photodenaturation induced by the UV harmonics of a Nd:YAP laser and by Nd:YAG laser at a wavelength of 1440 nm," Proc. SPIE 4161, 1-11 (2000).
[CrossRef]

McLaughlin, J.

R. F. Borkman and J. McLaughlin, "The molecular chaperone function of ?-crystallin is impaired by UV photolysis," Photochem. Photobiol. 62, 1046-1051 (1995). [Cross Ref]
[CrossRef] [PubMed]

Muraviov, S. V.

N. M. Bityurin, S. V. Muraviov, V. A. Kamensky, A. Y. Malyshev, E. V. Chelnokov, L. V. Soustov, and G. V. Gelikonov, "Kinetics of low-scattering biotissue photodenaturation induced by the UV harmonics of a Nd:YAP laser and by Nd:YAG laser at a wavelength of 1440 nm," Proc. SPIE 4161, 1-11 (2000).
[CrossRef]

Nemov, V. V.

L. V. Soustov, E. V. Chelnokov, N. M. Bityurin, V. V. Nemov, Yu. V. Sergeev, and M. A. Ostrovsky, "Photoaggregation of Water-soluble Protein (Carboanhydrase) Induced by the Ultraviolet Radiation of Xe-Cl Laser," Dokl. Biochem. Biophys. 388, 49-54 (2003).
[CrossRef] [PubMed]

Obi, B.

R. F. Borkman, G. Knight, and B. Obi, "The Molecular Chaperone ?-Crystallin Inhibits UV-Induced Protein Aggregation," Exp. Eye. Res. 62, 141-148 (1996). [Cross Ref]
[CrossRef] [PubMed]

Ostrovsky, M. A.

Yu. V. Sergeev, L. V. Soustov, E. V. Chelnokov, N. M. Bityurin, P. S. BacklundJr., P. T. Wingfield, M. A. Ostrovsky, and J. Fielding Hejtmancik, "Increased Sensitivity of Amino-Arm Truncated ßA3-Crystallin to UV-Light-Induced Photoaggregation," Invest Ophthalmol. Visual Sci. 46, 3263-3273 (2005). [Cross Ref]
[CrossRef]

L. V. Soustov, E. V. Chelnokov, N. M. Bityurin, V. V. Nemov, Yu. V. Sergeev, and M. A. Ostrovsky, "Photoaggregation of Water-soluble Protein (Carboanhydrase) Induced by the Ultraviolet Radiation of Xe-Cl Laser," Dokl. Biochem. Biophys. 388, 49-54 (2003).
[CrossRef] [PubMed]

M. A. Ostrovsky, I. B. Fedorovich, V. V. El'chaninov, and A. V. Krivandin, "Danger of damaging effect of light on the eye structures: Eye lens like a natural light filter and object of photodestruction," Sensor. Sistemy 8, 135-146 (1994).

M. M. Korkhmazyan, I. B. Fedorovich, and M. A. Ostrovsky, "Mechanisms of photodamage of eye structures. The effect of UV light on soluble lens proteins," Biofizika 28, 966-971 (1983).

Rothschild, M.

M. Rothschild and D. J. Ehrlich, "A review of excimer laser projection lithography," J. Vac. Sci. Technol. B 6, 1-17 (1988). [Cross Ref]
[CrossRef]

Sergeev, Yu. V.

Yu. V. Sergeev, L. V. Soustov, E. V. Chelnokov, N. M. Bityurin, P. S. BacklundJr., P. T. Wingfield, M. A. Ostrovsky, and J. Fielding Hejtmancik, "Increased Sensitivity of Amino-Arm Truncated ßA3-Crystallin to UV-Light-Induced Photoaggregation," Invest Ophthalmol. Visual Sci. 46, 3263-3273 (2005). [Cross Ref]
[CrossRef]

L. V. Soustov, E. V. Chelnokov, N. M. Bityurin, V. V. Nemov, Yu. V. Sergeev, and M. A. Ostrovsky, "Photoaggregation of Water-soluble Protein (Carboanhydrase) Induced by the Ultraviolet Radiation of Xe-Cl Laser," Dokl. Biochem. Biophys. 388, 49-54 (2003).
[CrossRef] [PubMed]

Shilov, A. A.

E. L. Bubis, V. V. Var'gin, L. R. Konchalina, and A. A. Shilov, "Study of low-absorption media for SBS in the near-IR spectral range," Opt. Spektrosk. 65, 1281-1285 (1988).

Soustov, L. V.

Yu. V. Sergeev, L. V. Soustov, E. V. Chelnokov, N. M. Bityurin, P. S. BacklundJr., P. T. Wingfield, M. A. Ostrovsky, and J. Fielding Hejtmancik, "Increased Sensitivity of Amino-Arm Truncated ßA3-Crystallin to UV-Light-Induced Photoaggregation," Invest Ophthalmol. Visual Sci. 46, 3263-3273 (2005). [Cross Ref]
[CrossRef]

L. V. Soustov, E. V. Chelnokov, N. M. Bityurin, V. V. Nemov, Yu. V. Sergeev, and M. A. Ostrovsky, "Photoaggregation of Water-soluble Protein (Carboanhydrase) Induced by the Ultraviolet Radiation of Xe-Cl Laser," Dokl. Biochem. Biophys. 388, 49-54 (2003).
[CrossRef] [PubMed]

N. M. Bityurin, S. V. Muraviov, V. A. Kamensky, A. Y. Malyshev, E. V. Chelnokov, L. V. Soustov, and G. V. Gelikonov, "Kinetics of low-scattering biotissue photodenaturation induced by the UV harmonics of a Nd:YAP laser and by Nd:YAG laser at a wavelength of 1440 nm," Proc. SPIE 4161, 1-11 (2000).
[CrossRef]

Sukharev, V. I.

N. L. Vekshin and V. I. Sukharev, "Thermo- and photoinduced aggregation of alpha-crystallin," Biofizika 50, 236-242 (2005).
[PubMed]

Var'gin, V. V.

E. L. Bubis, V. V. Var'gin, L. R. Konchalina, and A. A. Shilov, "Study of low-absorption media for SBS in the near-IR spectral range," Opt. Spektrosk. 65, 1281-1285 (1988).

Vekshin, N. L.

N. L. Vekshin and V. I. Sukharev, "Thermo- and photoinduced aggregation of alpha-crystallin," Biofizika 50, 236-242 (2005).
[PubMed]

Wingfield, P. T.

Yu. V. Sergeev, L. V. Soustov, E. V. Chelnokov, N. M. Bityurin, P. S. BacklundJr., P. T. Wingfield, M. A. Ostrovsky, and J. Fielding Hejtmancik, "Increased Sensitivity of Amino-Arm Truncated ßA3-Crystallin to UV-Light-Induced Photoaggregation," Invest Ophthalmol. Visual Sci. 46, 3263-3273 (2005). [Cross Ref]
[CrossRef]

Biochem. Biophys. Res. Commun. (1)

J.-H. Liao, J.-S. Lee, and S.-H. Chiou, "Distinct roles of ?A- and ?B-crystallins under thermal and UV stresses," Biochem. Biophys. Res. Commun. 295, 854-861 (2002). [Cross Ref]
[CrossRef] [PubMed]

Biofizika (3)

V. V. El'chaninov and I. B. Fedorovich, "Mechanisms of photodestruction of the eye structure. Formation of polypeptide aggregates upon UV-irradiation of lens proteins," Biofizika 34, 758-762 (1989).
[PubMed]

M. M. Korkhmazyan, I. B. Fedorovich, and M. A. Ostrovsky, "Mechanisms of photodamage of eye structures. The effect of UV light on soluble lens proteins," Biofizika 28, 966-971 (1983).

N. L. Vekshin and V. I. Sukharev, "Thermo- and photoinduced aggregation of alpha-crystallin," Biofizika 50, 236-242 (2005).
[PubMed]

Dokl. Biochem. Biophys. (1)

L. V. Soustov, E. V. Chelnokov, N. M. Bityurin, V. V. Nemov, Yu. V. Sergeev, and M. A. Ostrovsky, "Photoaggregation of Water-soluble Protein (Carboanhydrase) Induced by the Ultraviolet Radiation of Xe-Cl Laser," Dokl. Biochem. Biophys. 388, 49-54 (2003).
[CrossRef] [PubMed]

Exp. Eye Res. (1)

A. V. Krivandin, Yu. M. L'vov, M. A. Ostrovsky, I. B. Fedorovich, and L. A. Feigin, "Structural conversions of crystallins under senile cataract, dehydration and UV-irradiation studied by X-ray diffraction," Exp. Eye Res. 49, 853-859 (1989). [Cross Ref]
[CrossRef] [PubMed]

Exp. Eye. Res. (1)

R. F. Borkman, G. Knight, and B. Obi, "The Molecular Chaperone ?-Crystallin Inhibits UV-Induced Protein Aggregation," Exp. Eye. Res. 62, 141-148 (1996). [Cross Ref]
[CrossRef] [PubMed]

Invest Ophthalmol. Visual Sci. (1)

Yu. V. Sergeev, L. V. Soustov, E. V. Chelnokov, N. M. Bityurin, P. S. BacklundJr., P. T. Wingfield, M. A. Ostrovsky, and J. Fielding Hejtmancik, "Increased Sensitivity of Amino-Arm Truncated ßA3-Crystallin to UV-Light-Induced Photoaggregation," Invest Ophthalmol. Visual Sci. 46, 3263-3273 (2005). [Cross Ref]
[CrossRef]

J. Vac. Sci. Technol. B (1)

M. Rothschild and D. J. Ehrlich, "A review of excimer laser projection lithography," J. Vac. Sci. Technol. B 6, 1-17 (1988). [Cross Ref]
[CrossRef]

Opt. Spektrosk. (1)

E. L. Bubis, V. V. Var'gin, L. R. Konchalina, and A. A. Shilov, "Study of low-absorption media for SBS in the near-IR spectral range," Opt. Spektrosk. 65, 1281-1285 (1988).

Photochem. Photobiol. (1)

R. F. Borkman and J. McLaughlin, "The molecular chaperone function of ?-crystallin is impaired by UV photolysis," Photochem. Photobiol. 62, 1046-1051 (1995). [Cross Ref]
[CrossRef] [PubMed]

Proc. SPIE (1)

N. M. Bityurin, S. V. Muraviov, V. A. Kamensky, A. Y. Malyshev, E. V. Chelnokov, L. V. Soustov, and G. V. Gelikonov, "Kinetics of low-scattering biotissue photodenaturation induced by the UV harmonics of a Nd:YAP laser and by Nd:YAG laser at a wavelength of 1440 nm," Proc. SPIE 4161, 1-11 (2000).
[CrossRef]

Sistemy (1)

M. A. Ostrovsky, I. B. Fedorovich, V. V. El'chaninov, and A. V. Krivandin, "Danger of damaging effect of light on the eye structures: Eye lens like a natural light filter and object of photodestruction," Sensor. Sistemy 8, 135-146 (1994).

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Figures (3)

Fig. 1.
Fig. 1.

Experimental setup.

Fig. 2.
Fig. 2.

The scattering-exposure curves for the irradiation of a solution of β-crystallin with a concentration of n=0.5 mg/ml at a constant repetition rate (F), for different fluences (w) (a); and at constant fluence with different repetitions rates (b). In Fig. 2 a we also show the transmission curves at 308 nm. The abrupt change in the slope of the curves measured at different wavelengths takes place at approximately the same exposure. In Fig. 2 b the lag times for repetition rates 4, 2, 1.5, 1 and 0.7 Hz are 7.5, 18.5, 29, 49 and 95 min, correspondingly.

Fig. 3.
Fig. 3.

The experimental (symbols) and theoretical (dotted) results for D*(I) with I=F×w. Blue square symbols were obtained with w=const=75 mJ/cm2, red circles were obtained with F=const=2 Hz. Fitting parameter values are I 0=90 mW/cm2 and D 0=40 J/cm2 (see the model in the text).

Equations (9)

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dR dt = η σ N 10 I ω R τ k 2 R 2 ,
dM dt = k 2 R 2
R stat = ( 2 k 2 τ ) 1 × ( 1 + I I 0 1 )
I 0 = ω η σ N 10 · 1 4 k 2 τ 2
M = M *
D * D 0 I I 0 ( 1 + I I 0 1 ) 2 ,
D 0 = M * N 10 × ω η σ
D * D 0 ,
D * 4 D 0 I 0 I .

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