Abstract

Optically guided neuron growth is a relatively new field where the exact mechanisms that initiate growth are not well understood. Both Gaussian light beams and optical line traps have been purported to initiate neuronal growth. Here we present a detailed study using optical line traps with symmetric and asymmetric intensity profiles which have been previously reported to bias the direction of neuronal growth. In contrast to these previous studies, we show similar levels of growth regardless of the direction of the intensity variation along the line trap. Furthermore, our experimental observations confirm previous suggestions that the filopodia produced from neuronal growth cones can be affected by laser light. We experimentally observe alignment of filopodia with the laser field and present a theoretical model describing the optical torques experienced by filopodia to explain this effect.

© 2008 Optical Society of America

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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
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    [CrossRef]

2008 (3)

K. Dholakia, P. Reece, and M. Gu, "Optical micromanipulation," Chem. Soc. Rev. 37, 42-55 (2008).
[CrossRef]

Y. Lan and G. A. Papoian, "The stochastic dynamics of filopodial growth," Biophys. J. 94, 3839-3852 (2008).
[CrossRef]

D. J. Stevenson et al, "Long-term cell culture on a microscope stage: The Carrel Flask revisited," Microscopy Anal. 22, 9-11 (2008).

2007 (2)

Y. Cong et al, "Crystallographic conformers of actin in a biologically active bundle of filaments," J. Mol. Biol. 375, 331-336 (2007).
[CrossRef]

A. Ehrlicher, T. Betz, B. Stuhrmann, M. Gögler, D. Koch, K. Franze, Y. Lu, and J. Käs, "Optical neuronal guidance," Methods Cell Biol. 83, 495-520 (2007).
[CrossRef]

2006 (1)

2005 (2)

2003 (1)

E. W. Dent and F. B. Gertler, "Cytoskeletal dynamics and transport in growth cone motility and axon guidance," Neuron Vol. 40, 209-227 (2003).
[CrossRef]

2002 (1)

A. Ehrlicher, T. Betz, B. Stuhrmann, D. Koch, V. Milner, M. G. Raizen, and J. Kas, "Guiding neuronal growth with light," Proc. Nat. Acad. Sci. Vol. 9916024-16028 (2002).
[CrossRef]

2000 (1)

1991 (1)

G. Albrecht-Buehler, "Surface extensions of 3T3 cells towards distant infrared light sources," J. Cell. Biol. 114, 493-502 (1991).
[CrossRef]

1988 (1)

B. T. Draine, "The discrete-dipole approximation and its application to interstellar graphite grains," Astrophys. J. 333,848-872 (1988).

Agate, B.

Albrecht-Buehler, G.

G. Albrecht-Buehler, "Surface extensions of 3T3 cells towards distant infrared light sources," J. Cell. Biol. 114, 493-502 (1991).
[CrossRef]

Betz, T.

A. Ehrlicher, T. Betz, B. Stuhrmann, M. Gögler, D. Koch, K. Franze, Y. Lu, and J. Käs, "Optical neuronal guidance," Methods Cell Biol. 83, 495-520 (2007).
[CrossRef]

A. Ehrlicher, T. Betz, B. Stuhrmann, D. Koch, V. Milner, M. G. Raizen, and J. Kas, "Guiding neuronal growth with light," Proc. Nat. Acad. Sci. Vol. 9916024-16028 (2002).
[CrossRef]

Chaumet, P. C.

Cong, Y.

Y. Cong et al, "Crystallographic conformers of actin in a biologically active bundle of filaments," J. Mol. Biol. 375, 331-336 (2007).
[CrossRef]

Dent, E. W.

E. W. Dent and F. B. Gertler, "Cytoskeletal dynamics and transport in growth cone motility and axon guidance," Neuron Vol. 40, 209-227 (2003).
[CrossRef]

Dholakia, K.

Draine, B. T.

B. T. Draine, "The discrete-dipole approximation and its application to interstellar graphite grains," Astrophys. J. 333,848-872 (1988).

Ehrlicher, A.

A. Ehrlicher, T. Betz, B. Stuhrmann, M. Gögler, D. Koch, K. Franze, Y. Lu, and J. Käs, "Optical neuronal guidance," Methods Cell Biol. 83, 495-520 (2007).
[CrossRef]

A. Ehrlicher, T. Betz, B. Stuhrmann, D. Koch, V. Milner, M. G. Raizen, and J. Kas, "Guiding neuronal growth with light," Proc. Nat. Acad. Sci. Vol. 9916024-16028 (2002).
[CrossRef]

Franze, K.

A. Ehrlicher, T. Betz, B. Stuhrmann, M. Gögler, D. Koch, K. Franze, Y. Lu, and J. Käs, "Optical neuronal guidance," Methods Cell Biol. 83, 495-520 (2007).
[CrossRef]

Garcés-Chávez, V.

Gertler, F. B.

E. W. Dent and F. B. Gertler, "Cytoskeletal dynamics and transport in growth cone motility and axon guidance," Neuron Vol. 40, 209-227 (2003).
[CrossRef]

Gögler, M.

A. Ehrlicher, T. Betz, B. Stuhrmann, M. Gögler, D. Koch, K. Franze, Y. Lu, and J. Käs, "Optical neuronal guidance," Methods Cell Biol. 83, 495-520 (2007).
[CrossRef]

Gu, M.

K. Dholakia, P. Reece, and M. Gu, "Optical micromanipulation," Chem. Soc. Rev. 37, 42-55 (2008).
[CrossRef]

Gunn-Moore, F.

Gupta, P.

Kas, J.

A. Ehrlicher, T. Betz, B. Stuhrmann, D. Koch, V. Milner, M. G. Raizen, and J. Kas, "Guiding neuronal growth with light," Proc. Nat. Acad. Sci. Vol. 9916024-16028 (2002).
[CrossRef]

Käs, J.

A. Ehrlicher, T. Betz, B. Stuhrmann, M. Gögler, D. Koch, K. Franze, Y. Lu, and J. Käs, "Optical neuronal guidance," Methods Cell Biol. 83, 495-520 (2007).
[CrossRef]

Koch, D.

A. Ehrlicher, T. Betz, B. Stuhrmann, M. Gögler, D. Koch, K. Franze, Y. Lu, and J. Käs, "Optical neuronal guidance," Methods Cell Biol. 83, 495-520 (2007).
[CrossRef]

A. Ehrlicher, T. Betz, B. Stuhrmann, D. Koch, V. Milner, M. G. Raizen, and J. Kas, "Guiding neuronal growth with light," Proc. Nat. Acad. Sci. Vol. 9916024-16028 (2002).
[CrossRef]

Lake, T. K.

Lan, Y.

Y. Lan and G. A. Papoian, "The stochastic dynamics of filopodial growth," Biophys. J. 94, 3839-3852 (2008).
[CrossRef]

Lu, Y.

A. Ehrlicher, T. Betz, B. Stuhrmann, M. Gögler, D. Koch, K. Franze, Y. Lu, and J. Käs, "Optical neuronal guidance," Methods Cell Biol. 83, 495-520 (2007).
[CrossRef]

Milner, V.

A. Ehrlicher, T. Betz, B. Stuhrmann, D. Koch, V. Milner, M. G. Raizen, and J. Kas, "Guiding neuronal growth with light," Proc. Nat. Acad. Sci. Vol. 9916024-16028 (2002).
[CrossRef]

Mogilner, A.

A. Mogilner and B. Rubinstein, "The physics of filopodial protrusion," Biophys. J. 89, 782-795 (2005).
[CrossRef]

Mohanty, S.

Nieto-Vesperinas, M.

Panicker, M.

Papoian, G. A.

Y. Lan and G. A. Papoian, "The stochastic dynamics of filopodial growth," Biophys. J. 94, 3839-3852 (2008).
[CrossRef]

Raizen, M. G.

A. Ehrlicher, T. Betz, B. Stuhrmann, D. Koch, V. Milner, M. G. Raizen, and J. Kas, "Guiding neuronal growth with light," Proc. Nat. Acad. Sci. Vol. 9916024-16028 (2002).
[CrossRef]

Reece, P.

K. Dholakia, P. Reece, and M. Gu, "Optical micromanipulation," Chem. Soc. Rev. 37, 42-55 (2008).
[CrossRef]

Rubinstein, B.

A. Mogilner and B. Rubinstein, "The physics of filopodial protrusion," Biophys. J. 89, 782-795 (2005).
[CrossRef]

Sharma, M.

Stevenson, D. J.

Stuhrmann, B.

A. Ehrlicher, T. Betz, B. Stuhrmann, M. Gögler, D. Koch, K. Franze, Y. Lu, and J. Käs, "Optical neuronal guidance," Methods Cell Biol. 83, 495-520 (2007).
[CrossRef]

A. Ehrlicher, T. Betz, B. Stuhrmann, D. Koch, V. Milner, M. G. Raizen, and J. Kas, "Guiding neuronal growth with light," Proc. Nat. Acad. Sci. Vol. 9916024-16028 (2002).
[CrossRef]

Astrophys. J. (1)

B. T. Draine, "The discrete-dipole approximation and its application to interstellar graphite grains," Astrophys. J. 333,848-872 (1988).

Biophys. J. (2)

A. Mogilner and B. Rubinstein, "The physics of filopodial protrusion," Biophys. J. 89, 782-795 (2005).
[CrossRef]

Y. Lan and G. A. Papoian, "The stochastic dynamics of filopodial growth," Biophys. J. 94, 3839-3852 (2008).
[CrossRef]

Chem. Soc. Rev. (1)

K. Dholakia, P. Reece, and M. Gu, "Optical micromanipulation," Chem. Soc. Rev. 37, 42-55 (2008).
[CrossRef]

J. Cell. Biol. (1)

G. Albrecht-Buehler, "Surface extensions of 3T3 cells towards distant infrared light sources," J. Cell. Biol. 114, 493-502 (1991).
[CrossRef]

J. Mol. Biol. (1)

Y. Cong et al, "Crystallographic conformers of actin in a biologically active bundle of filaments," J. Mol. Biol. 375, 331-336 (2007).
[CrossRef]

Methods Cell Biol. (1)

A. Ehrlicher, T. Betz, B. Stuhrmann, M. Gögler, D. Koch, K. Franze, Y. Lu, and J. Käs, "Optical neuronal guidance," Methods Cell Biol. 83, 495-520 (2007).
[CrossRef]

Microscopy Anal. (1)

D. J. Stevenson et al, "Long-term cell culture on a microscope stage: The Carrel Flask revisited," Microscopy Anal. 22, 9-11 (2008).

Neuron Vol. (1)

E. W. Dent and F. B. Gertler, "Cytoskeletal dynamics and transport in growth cone motility and axon guidance," Neuron Vol. 40, 209-227 (2003).
[CrossRef]

Opt. Express (1)

Opt. Lett. (2)

Proc. Nat. Acad. Sci. Vol. (1)

A. Ehrlicher, T. Betz, B. Stuhrmann, D. Koch, V. Milner, M. G. Raizen, and J. Kas, "Guiding neuronal growth with light," Proc. Nat. Acad. Sci. Vol. 9916024-16028 (2002).
[CrossRef]

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