Abstract

Multiphoton microscopes have become important tools for non-contact sub-wavelength three-dimensional nanoprocessing of living biological specimens based on multiphoton ionization and plasma formation. Ultrashort laser pulses are required, however, dispersive effects limit the shortest pulse duration achievable at the focal plane. We report on a compact nonlinear laser scanning microscope with sub-20 femtosecond 75 MHz near infrared laser pulses for nanosurgery of human stem cells and two-photon high-resolution imaging. Single point illumination of the cell membrane was performed to induce a transient nanopore for the delivery of extracellular green fluorescent protein plasmids. Mean powers of less than 7 mW (<93 pJ) and low millisecond exposure times were found to be sufficient to transfect human pancreatic and salivary gland stem cells in these preliminary studies. Ultracompact sub-20 femtosecond laser microscopes may become optical tools for nanobiotechnology and nanomedicine including optical stem cell manipulation.

© 2008 Optical Society of America

PDF Article

References

  • View by:
  • |
  • |
  • |

  1. M. Müller, J. Squier, R. Wolleschensky, U. Simon, and J. Brakenhoff, "Dispersion pre compensation for high-numerical aperture objectives," J. Microsc. 191, 141-150 (1997).
    [CrossRef]
  2. J. Jasapara and W. Rudolph, "Characterization of sub-10-fs pulse focussing with high numerical aperture microscope objectives," Opt. Lett. 24, 777-779 (1999).
    [CrossRef]
  3. S. Tang, T. B. Krasieva, Z. Chen, G. Tempea, and B. J. Tromberg, "Increasing efficiency of two-photon excited fluorescence and second harmonic generation using ultrashort laser pulses," Proc. SPIE 6089, 308-315 (2006).
  4. G. McConnell and E. Riss, "Two-photon laser scanning microscopy using photonic crystal fiber," J. Biomed. Opt. 9, 922-927 (2004).
    [CrossRef]
  5. R. Szipöcs, K, Ferencz, C. Spielmann, and F. Krausz, "Chirped multilayer coatings for broadband dispersion control in femtosecond lasers," Opt. Lett. 19, 201-203 (1994).
  6. A. Stingl, C. Spielmann, F. Krausz, and R. Szipöcs, "Generation of 11-fs pulses from a Ti:sapphire laser without the use of prisms," Opt. Lett. 19, 204-206 (1994).
  7. F. W. Helbing, G. Steinmeyer, J. Stenger, H. R. Telle, and U. Keller, "Carrier-envelope-offset dynamics and stabilization of femtosecond pulses," Appl. Phys. B 74, S35-S42 (2002).
    [CrossRef]
  8. V. Venugopalan, A. Guerra, K. Nahen, and A. Vogel, "Role of laser-induced plasma formation in pulse cellular microsurgery and micromanipualtion," Phys. Rev. Lett. 88, 078103 (2002).
    [CrossRef]
  9. A. Vogel and V. Venugopalan, "Mechanism pulsed laser ablation of biological tissues," Chem Rev. 103, 577-644 (2003).
    [CrossRef]
  10. K. König, I. Riemann, and W. Fritzsche, "Nanodissection of human chromosomes with near infrared femtosecond laser pulses," Opt. Lett. 26, 819-821 (2001).
    [CrossRef]
  11. U. K. Tirlapur and K. König, "Femtosecond near-infrared laser pulses as versatile non-invasive tool for intratissue nanoprocessing in plants without compromising viability," The Plant Journal 31, 365-374 (2002).
    [CrossRef]
  12. K. König, O. Krauss, and I. Riemann, "Intratissue surgery with 80 MHz nanojoule femtosecond laser pulses in the near infrared," Opt. Express 10, 171-176 (2002).
  13. F. Stracke, I. Riemann, and K. König, "Optical nanoinjection of macromolecules into vital cells," J. Photochem. Photobiol. B. 81, 136-142 (2005).
    [CrossRef]
  14. K. König, I. Riemann, F. Stracke, and R. LeHarzic, "Nanoprocessing with nanojoule near-infrared femtosecond laser pulses," Med. Laser Appl. 20, 169-184 (2005).
    [CrossRef]
  15. A. Czaki, F. Garwe, A. Steinbrueck, G. Maubach, G. Festag, A. Weise, I. Riemann, K. König, and W. Fritzsche, "A parallel approach for sub-wavelength molecular surgery using gene-specific positioned metal nanoparticles as laser light antennas," Nanoletters 2, 247-253 (2007).
  16. W. Watanabe, S. Matsunaga, T. Shimada, T. Higashi, K. Fukui, and K. Itoh, "Femtosecond laser disruption of mitochondria in living cells," Med. Laser Appl. 20, 185-191 (2005).
    [CrossRef]
  17. V. Kohli, A. Elezzabi, and J. P. Acker, "Cell nanosurgery using ultrashort (femtosecond) laser pulses: applications to membrane surgery and cell isolation," Lasers Surg. Med. 37, 227-230 (2005).
    [CrossRef]
  18. L. Sacconi, I. Tolic-Norrelykke, R. Antolini, and F. S. Pavone, "Combined intracellualar three-dimensional imaging and selective nanosurgery by a nonlinear microscope," J. Biomed. Opt. 10, 14002 (2005).
    [CrossRef]
  19. M. F. Yanik, H. Cinar, H. N. Cinar, A. D. Chisholm, Y. Jin, and A. Ben-Yakar, "Nanosurgery: functional regeneration after laser axetomy," Nature 432, 822 (2004).
    [CrossRef]
  20. U. K. Tirpalur and K. König, "Targeted transfection by femtosecond laser," Nature 418, 4295-4298 (2002).
  21. X. Tsampoula, V. Garces-Chavez, M. Comrie, D. J. Stevenson, B. Agate, C. T. A. Brown, F. Gunn-Moore, and K. Dholakia, "Femtosecond cellular transfection using a nondiffracting light beam," Appl. Phys. Lett. 91, 053902 (2007).
    [CrossRef]
  22. D. Stevenson, B. Agate, X. Tsampoula, P. Fischer, C. T. A. Brown, W. Sibbett, A. Riches, F. Gunn-Moore, and K. Dholakia, "Femtosecond optical transfection of cells: viability and efficiency," Opt. Express 14, 7125-7133 (2006).
    [CrossRef]
  23. A. Hamm, N. Krott, I. Breibach, R. Blindt, and A. K. Bosserhof, "Efficient transfection method for primary cells," Tissue Engineering 8, 1-13 (2002).
    [CrossRef]
  24. H. Haleem-Smith, A. Derfoul, C. Okafor, R. Tuli, D. Olsen, D. J. Hall, and R. S. Tuan, "Optimization of high-efficiency transfection of adult human mesenchymal stem cells in vitro," Mol. Biotech. 3, 09-19 (2005).
    [CrossRef]
  25. A. M. Larson and A. T. Yeh, "Ex vivo characterization of sub-10 fs pulses," Opt. Lett. 31,1681-1683 (2006).
    [CrossRef]
  26. G. Tempea, B. Považay, A. Assion, A. Isemann, W. Pervak, M. Kempe, A. Stingl, and W. Drexler, "Undistored delivery of sub-15 fs pulses via high-numerical-aperture microscope objectives," Proc. SPIE 6442, 64420P-1-5 (2007).
  27. C. Kruse, M. Birth, J. Rohwedel, K. Assmuth, A. Goepel, and T. Wedel, "Pluripotency of adult stem cells derived from human and rat pancreas," Appl. Phys. A. 79, 1617-1624 (2004).
  28. C. Kruse, J. Kajahn, A. E. Petschnik, A. Maas, E. Klink, D. H. Rapoport, and T. Wedel, "Adult pancreatic stem/progenitor cells spontaneously differentiate in vitro into multiple cell lineages and form teratoma-like structures," Annals of Anatomy 188, 503-517 (2006).
    [CrossRef]

2007 (2)

A. Czaki, F. Garwe, A. Steinbrueck, G. Maubach, G. Festag, A. Weise, I. Riemann, K. König, and W. Fritzsche, "A parallel approach for sub-wavelength molecular surgery using gene-specific positioned metal nanoparticles as laser light antennas," Nanoletters 2, 247-253 (2007).

X. Tsampoula, V. Garces-Chavez, M. Comrie, D. J. Stevenson, B. Agate, C. T. A. Brown, F. Gunn-Moore, and K. Dholakia, "Femtosecond cellular transfection using a nondiffracting light beam," Appl. Phys. Lett. 91, 053902 (2007).
[CrossRef]

2006 (4)

S. Tang, T. B. Krasieva, Z. Chen, G. Tempea, and B. J. Tromberg, "Increasing efficiency of two-photon excited fluorescence and second harmonic generation using ultrashort laser pulses," Proc. SPIE 6089, 308-315 (2006).

C. Kruse, J. Kajahn, A. E. Petschnik, A. Maas, E. Klink, D. H. Rapoport, and T. Wedel, "Adult pancreatic stem/progenitor cells spontaneously differentiate in vitro into multiple cell lineages and form teratoma-like structures," Annals of Anatomy 188, 503-517 (2006).
[CrossRef]

A. M. Larson and A. T. Yeh, "Ex vivo characterization of sub-10 fs pulses," Opt. Lett. 31,1681-1683 (2006).
[CrossRef]

D. Stevenson, B. Agate, X. Tsampoula, P. Fischer, C. T. A. Brown, W. Sibbett, A. Riches, F. Gunn-Moore, and K. Dholakia, "Femtosecond optical transfection of cells: viability and efficiency," Opt. Express 14, 7125-7133 (2006).
[CrossRef]

2005 (6)

W. Watanabe, S. Matsunaga, T. Shimada, T. Higashi, K. Fukui, and K. Itoh, "Femtosecond laser disruption of mitochondria in living cells," Med. Laser Appl. 20, 185-191 (2005).
[CrossRef]

V. Kohli, A. Elezzabi, and J. P. Acker, "Cell nanosurgery using ultrashort (femtosecond) laser pulses: applications to membrane surgery and cell isolation," Lasers Surg. Med. 37, 227-230 (2005).
[CrossRef]

L. Sacconi, I. Tolic-Norrelykke, R. Antolini, and F. S. Pavone, "Combined intracellualar three-dimensional imaging and selective nanosurgery by a nonlinear microscope," J. Biomed. Opt. 10, 14002 (2005).
[CrossRef]

F. Stracke, I. Riemann, and K. König, "Optical nanoinjection of macromolecules into vital cells," J. Photochem. Photobiol. B. 81, 136-142 (2005).
[CrossRef]

K. König, I. Riemann, F. Stracke, and R. LeHarzic, "Nanoprocessing with nanojoule near-infrared femtosecond laser pulses," Med. Laser Appl. 20, 169-184 (2005).
[CrossRef]

H. Haleem-Smith, A. Derfoul, C. Okafor, R. Tuli, D. Olsen, D. J. Hall, and R. S. Tuan, "Optimization of high-efficiency transfection of adult human mesenchymal stem cells in vitro," Mol. Biotech. 3, 09-19 (2005).
[CrossRef]

2004 (3)

C. Kruse, M. Birth, J. Rohwedel, K. Assmuth, A. Goepel, and T. Wedel, "Pluripotency of adult stem cells derived from human and rat pancreas," Appl. Phys. A. 79, 1617-1624 (2004).

M. F. Yanik, H. Cinar, H. N. Cinar, A. D. Chisholm, Y. Jin, and A. Ben-Yakar, "Nanosurgery: functional regeneration after laser axetomy," Nature 432, 822 (2004).
[CrossRef]

G. McConnell and E. Riss, "Two-photon laser scanning microscopy using photonic crystal fiber," J. Biomed. Opt. 9, 922-927 (2004).
[CrossRef]

2003 (1)

A. Vogel and V. Venugopalan, "Mechanism pulsed laser ablation of biological tissues," Chem Rev. 103, 577-644 (2003).
[CrossRef]

2002 (6)

K. König, O. Krauss, and I. Riemann, "Intratissue surgery with 80 MHz nanojoule femtosecond laser pulses in the near infrared," Opt. Express 10, 171-176 (2002).

F. W. Helbing, G. Steinmeyer, J. Stenger, H. R. Telle, and U. Keller, "Carrier-envelope-offset dynamics and stabilization of femtosecond pulses," Appl. Phys. B 74, S35-S42 (2002).
[CrossRef]

V. Venugopalan, A. Guerra, K. Nahen, and A. Vogel, "Role of laser-induced plasma formation in pulse cellular microsurgery and micromanipualtion," Phys. Rev. Lett. 88, 078103 (2002).
[CrossRef]

U. K. Tirpalur and K. König, "Targeted transfection by femtosecond laser," Nature 418, 4295-4298 (2002).

U. K. Tirlapur and K. König, "Femtosecond near-infrared laser pulses as versatile non-invasive tool for intratissue nanoprocessing in plants without compromising viability," The Plant Journal 31, 365-374 (2002).
[CrossRef]

A. Hamm, N. Krott, I. Breibach, R. Blindt, and A. K. Bosserhof, "Efficient transfection method for primary cells," Tissue Engineering 8, 1-13 (2002).
[CrossRef]

2001 (1)

1999 (1)

1997 (1)

M. Müller, J. Squier, R. Wolleschensky, U. Simon, and J. Brakenhoff, "Dispersion pre compensation for high-numerical aperture objectives," J. Microsc. 191, 141-150 (1997).
[CrossRef]

1994 (2)

Acker, J. P.

V. Kohli, A. Elezzabi, and J. P. Acker, "Cell nanosurgery using ultrashort (femtosecond) laser pulses: applications to membrane surgery and cell isolation," Lasers Surg. Med. 37, 227-230 (2005).
[CrossRef]

Agate, B.

X. Tsampoula, V. Garces-Chavez, M. Comrie, D. J. Stevenson, B. Agate, C. T. A. Brown, F. Gunn-Moore, and K. Dholakia, "Femtosecond cellular transfection using a nondiffracting light beam," Appl. Phys. Lett. 91, 053902 (2007).
[CrossRef]

D. Stevenson, B. Agate, X. Tsampoula, P. Fischer, C. T. A. Brown, W. Sibbett, A. Riches, F. Gunn-Moore, and K. Dholakia, "Femtosecond optical transfection of cells: viability and efficiency," Opt. Express 14, 7125-7133 (2006).
[CrossRef]

Antolini, R.

L. Sacconi, I. Tolic-Norrelykke, R. Antolini, and F. S. Pavone, "Combined intracellualar three-dimensional imaging and selective nanosurgery by a nonlinear microscope," J. Biomed. Opt. 10, 14002 (2005).
[CrossRef]

Assmuth, K.

C. Kruse, M. Birth, J. Rohwedel, K. Assmuth, A. Goepel, and T. Wedel, "Pluripotency of adult stem cells derived from human and rat pancreas," Appl. Phys. A. 79, 1617-1624 (2004).

Ben-Yakar, A.

M. F. Yanik, H. Cinar, H. N. Cinar, A. D. Chisholm, Y. Jin, and A. Ben-Yakar, "Nanosurgery: functional regeneration after laser axetomy," Nature 432, 822 (2004).
[CrossRef]

Birth, M.

C. Kruse, M. Birth, J. Rohwedel, K. Assmuth, A. Goepel, and T. Wedel, "Pluripotency of adult stem cells derived from human and rat pancreas," Appl. Phys. A. 79, 1617-1624 (2004).

Blindt, R.

A. Hamm, N. Krott, I. Breibach, R. Blindt, and A. K. Bosserhof, "Efficient transfection method for primary cells," Tissue Engineering 8, 1-13 (2002).
[CrossRef]

Bosserhof, A. K.

A. Hamm, N. Krott, I. Breibach, R. Blindt, and A. K. Bosserhof, "Efficient transfection method for primary cells," Tissue Engineering 8, 1-13 (2002).
[CrossRef]

Brakenhoff, J.

M. Müller, J. Squier, R. Wolleschensky, U. Simon, and J. Brakenhoff, "Dispersion pre compensation for high-numerical aperture objectives," J. Microsc. 191, 141-150 (1997).
[CrossRef]

Breibach, I.

A. Hamm, N. Krott, I. Breibach, R. Blindt, and A. K. Bosserhof, "Efficient transfection method for primary cells," Tissue Engineering 8, 1-13 (2002).
[CrossRef]

Brown, C. T. A.

X. Tsampoula, V. Garces-Chavez, M. Comrie, D. J. Stevenson, B. Agate, C. T. A. Brown, F. Gunn-Moore, and K. Dholakia, "Femtosecond cellular transfection using a nondiffracting light beam," Appl. Phys. Lett. 91, 053902 (2007).
[CrossRef]

D. Stevenson, B. Agate, X. Tsampoula, P. Fischer, C. T. A. Brown, W. Sibbett, A. Riches, F. Gunn-Moore, and K. Dholakia, "Femtosecond optical transfection of cells: viability and efficiency," Opt. Express 14, 7125-7133 (2006).
[CrossRef]

Chen, Z.

S. Tang, T. B. Krasieva, Z. Chen, G. Tempea, and B. J. Tromberg, "Increasing efficiency of two-photon excited fluorescence and second harmonic generation using ultrashort laser pulses," Proc. SPIE 6089, 308-315 (2006).

Chisholm, A. D.

M. F. Yanik, H. Cinar, H. N. Cinar, A. D. Chisholm, Y. Jin, and A. Ben-Yakar, "Nanosurgery: functional regeneration after laser axetomy," Nature 432, 822 (2004).
[CrossRef]

Cinar, H.

M. F. Yanik, H. Cinar, H. N. Cinar, A. D. Chisholm, Y. Jin, and A. Ben-Yakar, "Nanosurgery: functional regeneration after laser axetomy," Nature 432, 822 (2004).
[CrossRef]

Cinar, H. N.

M. F. Yanik, H. Cinar, H. N. Cinar, A. D. Chisholm, Y. Jin, and A. Ben-Yakar, "Nanosurgery: functional regeneration after laser axetomy," Nature 432, 822 (2004).
[CrossRef]

Comrie, M.

X. Tsampoula, V. Garces-Chavez, M. Comrie, D. J. Stevenson, B. Agate, C. T. A. Brown, F. Gunn-Moore, and K. Dholakia, "Femtosecond cellular transfection using a nondiffracting light beam," Appl. Phys. Lett. 91, 053902 (2007).
[CrossRef]

Czaki, A.

A. Czaki, F. Garwe, A. Steinbrueck, G. Maubach, G. Festag, A. Weise, I. Riemann, K. König, and W. Fritzsche, "A parallel approach for sub-wavelength molecular surgery using gene-specific positioned metal nanoparticles as laser light antennas," Nanoletters 2, 247-253 (2007).

Derfoul, A.

H. Haleem-Smith, A. Derfoul, C. Okafor, R. Tuli, D. Olsen, D. J. Hall, and R. S. Tuan, "Optimization of high-efficiency transfection of adult human mesenchymal stem cells in vitro," Mol. Biotech. 3, 09-19 (2005).
[CrossRef]

Dholakia, K.

X. Tsampoula, V. Garces-Chavez, M. Comrie, D. J. Stevenson, B. Agate, C. T. A. Brown, F. Gunn-Moore, and K. Dholakia, "Femtosecond cellular transfection using a nondiffracting light beam," Appl. Phys. Lett. 91, 053902 (2007).
[CrossRef]

D. Stevenson, B. Agate, X. Tsampoula, P. Fischer, C. T. A. Brown, W. Sibbett, A. Riches, F. Gunn-Moore, and K. Dholakia, "Femtosecond optical transfection of cells: viability and efficiency," Opt. Express 14, 7125-7133 (2006).
[CrossRef]

Elezzabi, A.

V. Kohli, A. Elezzabi, and J. P. Acker, "Cell nanosurgery using ultrashort (femtosecond) laser pulses: applications to membrane surgery and cell isolation," Lasers Surg. Med. 37, 227-230 (2005).
[CrossRef]

Festag, G.

A. Czaki, F. Garwe, A. Steinbrueck, G. Maubach, G. Festag, A. Weise, I. Riemann, K. König, and W. Fritzsche, "A parallel approach for sub-wavelength molecular surgery using gene-specific positioned metal nanoparticles as laser light antennas," Nanoletters 2, 247-253 (2007).

Fischer, P.

Fritzsche, W.

A. Czaki, F. Garwe, A. Steinbrueck, G. Maubach, G. Festag, A. Weise, I. Riemann, K. König, and W. Fritzsche, "A parallel approach for sub-wavelength molecular surgery using gene-specific positioned metal nanoparticles as laser light antennas," Nanoletters 2, 247-253 (2007).

K. König, I. Riemann, and W. Fritzsche, "Nanodissection of human chromosomes with near infrared femtosecond laser pulses," Opt. Lett. 26, 819-821 (2001).
[CrossRef]

Fukui, K.

W. Watanabe, S. Matsunaga, T. Shimada, T. Higashi, K. Fukui, and K. Itoh, "Femtosecond laser disruption of mitochondria in living cells," Med. Laser Appl. 20, 185-191 (2005).
[CrossRef]

Garces-Chavez, V.

X. Tsampoula, V. Garces-Chavez, M. Comrie, D. J. Stevenson, B. Agate, C. T. A. Brown, F. Gunn-Moore, and K. Dholakia, "Femtosecond cellular transfection using a nondiffracting light beam," Appl. Phys. Lett. 91, 053902 (2007).
[CrossRef]

Garwe, F.

A. Czaki, F. Garwe, A. Steinbrueck, G. Maubach, G. Festag, A. Weise, I. Riemann, K. König, and W. Fritzsche, "A parallel approach for sub-wavelength molecular surgery using gene-specific positioned metal nanoparticles as laser light antennas," Nanoletters 2, 247-253 (2007).

Goepel, A.

C. Kruse, M. Birth, J. Rohwedel, K. Assmuth, A. Goepel, and T. Wedel, "Pluripotency of adult stem cells derived from human and rat pancreas," Appl. Phys. A. 79, 1617-1624 (2004).

Guerra, A.

V. Venugopalan, A. Guerra, K. Nahen, and A. Vogel, "Role of laser-induced plasma formation in pulse cellular microsurgery and micromanipualtion," Phys. Rev. Lett. 88, 078103 (2002).
[CrossRef]

Gunn-Moore, F.

X. Tsampoula, V. Garces-Chavez, M. Comrie, D. J. Stevenson, B. Agate, C. T. A. Brown, F. Gunn-Moore, and K. Dholakia, "Femtosecond cellular transfection using a nondiffracting light beam," Appl. Phys. Lett. 91, 053902 (2007).
[CrossRef]

D. Stevenson, B. Agate, X. Tsampoula, P. Fischer, C. T. A. Brown, W. Sibbett, A. Riches, F. Gunn-Moore, and K. Dholakia, "Femtosecond optical transfection of cells: viability and efficiency," Opt. Express 14, 7125-7133 (2006).
[CrossRef]

Haleem-Smith, H.

H. Haleem-Smith, A. Derfoul, C. Okafor, R. Tuli, D. Olsen, D. J. Hall, and R. S. Tuan, "Optimization of high-efficiency transfection of adult human mesenchymal stem cells in vitro," Mol. Biotech. 3, 09-19 (2005).
[CrossRef]

Hall, D. J.

H. Haleem-Smith, A. Derfoul, C. Okafor, R. Tuli, D. Olsen, D. J. Hall, and R. S. Tuan, "Optimization of high-efficiency transfection of adult human mesenchymal stem cells in vitro," Mol. Biotech. 3, 09-19 (2005).
[CrossRef]

Hamm, A.

A. Hamm, N. Krott, I. Breibach, R. Blindt, and A. K. Bosserhof, "Efficient transfection method for primary cells," Tissue Engineering 8, 1-13 (2002).
[CrossRef]

Helbing, F. W.

F. W. Helbing, G. Steinmeyer, J. Stenger, H. R. Telle, and U. Keller, "Carrier-envelope-offset dynamics and stabilization of femtosecond pulses," Appl. Phys. B 74, S35-S42 (2002).
[CrossRef]

Higashi, T.

W. Watanabe, S. Matsunaga, T. Shimada, T. Higashi, K. Fukui, and K. Itoh, "Femtosecond laser disruption of mitochondria in living cells," Med. Laser Appl. 20, 185-191 (2005).
[CrossRef]

Itoh, K.

W. Watanabe, S. Matsunaga, T. Shimada, T. Higashi, K. Fukui, and K. Itoh, "Femtosecond laser disruption of mitochondria in living cells," Med. Laser Appl. 20, 185-191 (2005).
[CrossRef]

Jasapara, J.

Jin, Y.

M. F. Yanik, H. Cinar, H. N. Cinar, A. D. Chisholm, Y. Jin, and A. Ben-Yakar, "Nanosurgery: functional regeneration after laser axetomy," Nature 432, 822 (2004).
[CrossRef]

Kajahn, J.

C. Kruse, J. Kajahn, A. E. Petschnik, A. Maas, E. Klink, D. H. Rapoport, and T. Wedel, "Adult pancreatic stem/progenitor cells spontaneously differentiate in vitro into multiple cell lineages and form teratoma-like structures," Annals of Anatomy 188, 503-517 (2006).
[CrossRef]

Keller, U.

F. W. Helbing, G. Steinmeyer, J. Stenger, H. R. Telle, and U. Keller, "Carrier-envelope-offset dynamics and stabilization of femtosecond pulses," Appl. Phys. B 74, S35-S42 (2002).
[CrossRef]

Klink, E.

C. Kruse, J. Kajahn, A. E. Petschnik, A. Maas, E. Klink, D. H. Rapoport, and T. Wedel, "Adult pancreatic stem/progenitor cells spontaneously differentiate in vitro into multiple cell lineages and form teratoma-like structures," Annals of Anatomy 188, 503-517 (2006).
[CrossRef]

Kohli, V.

V. Kohli, A. Elezzabi, and J. P. Acker, "Cell nanosurgery using ultrashort (femtosecond) laser pulses: applications to membrane surgery and cell isolation," Lasers Surg. Med. 37, 227-230 (2005).
[CrossRef]

König, K.

A. Czaki, F. Garwe, A. Steinbrueck, G. Maubach, G. Festag, A. Weise, I. Riemann, K. König, and W. Fritzsche, "A parallel approach for sub-wavelength molecular surgery using gene-specific positioned metal nanoparticles as laser light antennas," Nanoletters 2, 247-253 (2007).

F. Stracke, I. Riemann, and K. König, "Optical nanoinjection of macromolecules into vital cells," J. Photochem. Photobiol. B. 81, 136-142 (2005).
[CrossRef]

K. König, I. Riemann, F. Stracke, and R. LeHarzic, "Nanoprocessing with nanojoule near-infrared femtosecond laser pulses," Med. Laser Appl. 20, 169-184 (2005).
[CrossRef]

U. K. Tirlapur and K. König, "Femtosecond near-infrared laser pulses as versatile non-invasive tool for intratissue nanoprocessing in plants without compromising viability," The Plant Journal 31, 365-374 (2002).
[CrossRef]

U. K. Tirpalur and K. König, "Targeted transfection by femtosecond laser," Nature 418, 4295-4298 (2002).

K. König, O. Krauss, and I. Riemann, "Intratissue surgery with 80 MHz nanojoule femtosecond laser pulses in the near infrared," Opt. Express 10, 171-176 (2002).

K. König, I. Riemann, and W. Fritzsche, "Nanodissection of human chromosomes with near infrared femtosecond laser pulses," Opt. Lett. 26, 819-821 (2001).
[CrossRef]

Krasieva, T. B.

S. Tang, T. B. Krasieva, Z. Chen, G. Tempea, and B. J. Tromberg, "Increasing efficiency of two-photon excited fluorescence and second harmonic generation using ultrashort laser pulses," Proc. SPIE 6089, 308-315 (2006).

Krauss, O.

Krausz, F.

Krott, N.

A. Hamm, N. Krott, I. Breibach, R. Blindt, and A. K. Bosserhof, "Efficient transfection method for primary cells," Tissue Engineering 8, 1-13 (2002).
[CrossRef]

Kruse, C.

C. Kruse, J. Kajahn, A. E. Petschnik, A. Maas, E. Klink, D. H. Rapoport, and T. Wedel, "Adult pancreatic stem/progenitor cells spontaneously differentiate in vitro into multiple cell lineages and form teratoma-like structures," Annals of Anatomy 188, 503-517 (2006).
[CrossRef]

C. Kruse, M. Birth, J. Rohwedel, K. Assmuth, A. Goepel, and T. Wedel, "Pluripotency of adult stem cells derived from human and rat pancreas," Appl. Phys. A. 79, 1617-1624 (2004).

Larson, A. M.

LeHarzic, R.

K. König, I. Riemann, F. Stracke, and R. LeHarzic, "Nanoprocessing with nanojoule near-infrared femtosecond laser pulses," Med. Laser Appl. 20, 169-184 (2005).
[CrossRef]

Maas, A.

C. Kruse, J. Kajahn, A. E. Petschnik, A. Maas, E. Klink, D. H. Rapoport, and T. Wedel, "Adult pancreatic stem/progenitor cells spontaneously differentiate in vitro into multiple cell lineages and form teratoma-like structures," Annals of Anatomy 188, 503-517 (2006).
[CrossRef]

Matsunaga, S.

W. Watanabe, S. Matsunaga, T. Shimada, T. Higashi, K. Fukui, and K. Itoh, "Femtosecond laser disruption of mitochondria in living cells," Med. Laser Appl. 20, 185-191 (2005).
[CrossRef]

Maubach, G.

A. Czaki, F. Garwe, A. Steinbrueck, G. Maubach, G. Festag, A. Weise, I. Riemann, K. König, and W. Fritzsche, "A parallel approach for sub-wavelength molecular surgery using gene-specific positioned metal nanoparticles as laser light antennas," Nanoletters 2, 247-253 (2007).

McConnell, G.

G. McConnell and E. Riss, "Two-photon laser scanning microscopy using photonic crystal fiber," J. Biomed. Opt. 9, 922-927 (2004).
[CrossRef]

Müller, M.

M. Müller, J. Squier, R. Wolleschensky, U. Simon, and J. Brakenhoff, "Dispersion pre compensation for high-numerical aperture objectives," J. Microsc. 191, 141-150 (1997).
[CrossRef]

Nahen, K.

V. Venugopalan, A. Guerra, K. Nahen, and A. Vogel, "Role of laser-induced plasma formation in pulse cellular microsurgery and micromanipualtion," Phys. Rev. Lett. 88, 078103 (2002).
[CrossRef]

Okafor, C.

H. Haleem-Smith, A. Derfoul, C. Okafor, R. Tuli, D. Olsen, D. J. Hall, and R. S. Tuan, "Optimization of high-efficiency transfection of adult human mesenchymal stem cells in vitro," Mol. Biotech. 3, 09-19 (2005).
[CrossRef]

Olsen, D.

H. Haleem-Smith, A. Derfoul, C. Okafor, R. Tuli, D. Olsen, D. J. Hall, and R. S. Tuan, "Optimization of high-efficiency transfection of adult human mesenchymal stem cells in vitro," Mol. Biotech. 3, 09-19 (2005).
[CrossRef]

Pavone, F. S.

L. Sacconi, I. Tolic-Norrelykke, R. Antolini, and F. S. Pavone, "Combined intracellualar three-dimensional imaging and selective nanosurgery by a nonlinear microscope," J. Biomed. Opt. 10, 14002 (2005).
[CrossRef]

Petschnik, A. E.

C. Kruse, J. Kajahn, A. E. Petschnik, A. Maas, E. Klink, D. H. Rapoport, and T. Wedel, "Adult pancreatic stem/progenitor cells spontaneously differentiate in vitro into multiple cell lineages and form teratoma-like structures," Annals of Anatomy 188, 503-517 (2006).
[CrossRef]

Rapoport, D. H.

C. Kruse, J. Kajahn, A. E. Petschnik, A. Maas, E. Klink, D. H. Rapoport, and T. Wedel, "Adult pancreatic stem/progenitor cells spontaneously differentiate in vitro into multiple cell lineages and form teratoma-like structures," Annals of Anatomy 188, 503-517 (2006).
[CrossRef]

Riches, A.

Riemann, I.

A. Czaki, F. Garwe, A. Steinbrueck, G. Maubach, G. Festag, A. Weise, I. Riemann, K. König, and W. Fritzsche, "A parallel approach for sub-wavelength molecular surgery using gene-specific positioned metal nanoparticles as laser light antennas," Nanoletters 2, 247-253 (2007).

K. König, I. Riemann, F. Stracke, and R. LeHarzic, "Nanoprocessing with nanojoule near-infrared femtosecond laser pulses," Med. Laser Appl. 20, 169-184 (2005).
[CrossRef]

F. Stracke, I. Riemann, and K. König, "Optical nanoinjection of macromolecules into vital cells," J. Photochem. Photobiol. B. 81, 136-142 (2005).
[CrossRef]

K. König, O. Krauss, and I. Riemann, "Intratissue surgery with 80 MHz nanojoule femtosecond laser pulses in the near infrared," Opt. Express 10, 171-176 (2002).

K. König, I. Riemann, and W. Fritzsche, "Nanodissection of human chromosomes with near infrared femtosecond laser pulses," Opt. Lett. 26, 819-821 (2001).
[CrossRef]

Riss, E.

G. McConnell and E. Riss, "Two-photon laser scanning microscopy using photonic crystal fiber," J. Biomed. Opt. 9, 922-927 (2004).
[CrossRef]

Rohwedel, J.

C. Kruse, M. Birth, J. Rohwedel, K. Assmuth, A. Goepel, and T. Wedel, "Pluripotency of adult stem cells derived from human and rat pancreas," Appl. Phys. A. 79, 1617-1624 (2004).

Rudolph, W.

Sacconi, L.

L. Sacconi, I. Tolic-Norrelykke, R. Antolini, and F. S. Pavone, "Combined intracellualar three-dimensional imaging and selective nanosurgery by a nonlinear microscope," J. Biomed. Opt. 10, 14002 (2005).
[CrossRef]

Shimada, T.

W. Watanabe, S. Matsunaga, T. Shimada, T. Higashi, K. Fukui, and K. Itoh, "Femtosecond laser disruption of mitochondria in living cells," Med. Laser Appl. 20, 185-191 (2005).
[CrossRef]

Sibbett, W.

Simon, U.

M. Müller, J. Squier, R. Wolleschensky, U. Simon, and J. Brakenhoff, "Dispersion pre compensation for high-numerical aperture objectives," J. Microsc. 191, 141-150 (1997).
[CrossRef]

Spielmann, C.

Squier, J.

M. Müller, J. Squier, R. Wolleschensky, U. Simon, and J. Brakenhoff, "Dispersion pre compensation for high-numerical aperture objectives," J. Microsc. 191, 141-150 (1997).
[CrossRef]

Steinbrueck, A.

A. Czaki, F. Garwe, A. Steinbrueck, G. Maubach, G. Festag, A. Weise, I. Riemann, K. König, and W. Fritzsche, "A parallel approach for sub-wavelength molecular surgery using gene-specific positioned metal nanoparticles as laser light antennas," Nanoletters 2, 247-253 (2007).

Steinmeyer, G.

F. W. Helbing, G. Steinmeyer, J. Stenger, H. R. Telle, and U. Keller, "Carrier-envelope-offset dynamics and stabilization of femtosecond pulses," Appl. Phys. B 74, S35-S42 (2002).
[CrossRef]

Stenger, J.

F. W. Helbing, G. Steinmeyer, J. Stenger, H. R. Telle, and U. Keller, "Carrier-envelope-offset dynamics and stabilization of femtosecond pulses," Appl. Phys. B 74, S35-S42 (2002).
[CrossRef]

Stevenson, D.

Stevenson, D. J.

X. Tsampoula, V. Garces-Chavez, M. Comrie, D. J. Stevenson, B. Agate, C. T. A. Brown, F. Gunn-Moore, and K. Dholakia, "Femtosecond cellular transfection using a nondiffracting light beam," Appl. Phys. Lett. 91, 053902 (2007).
[CrossRef]

Stingl, A.

Stracke, F.

F. Stracke, I. Riemann, and K. König, "Optical nanoinjection of macromolecules into vital cells," J. Photochem. Photobiol. B. 81, 136-142 (2005).
[CrossRef]

K. König, I. Riemann, F. Stracke, and R. LeHarzic, "Nanoprocessing with nanojoule near-infrared femtosecond laser pulses," Med. Laser Appl. 20, 169-184 (2005).
[CrossRef]

Szipöcs, R.

Tang, S.

S. Tang, T. B. Krasieva, Z. Chen, G. Tempea, and B. J. Tromberg, "Increasing efficiency of two-photon excited fluorescence and second harmonic generation using ultrashort laser pulses," Proc. SPIE 6089, 308-315 (2006).

Telle, H. R.

F. W. Helbing, G. Steinmeyer, J. Stenger, H. R. Telle, and U. Keller, "Carrier-envelope-offset dynamics and stabilization of femtosecond pulses," Appl. Phys. B 74, S35-S42 (2002).
[CrossRef]

Tempea, G.

S. Tang, T. B. Krasieva, Z. Chen, G. Tempea, and B. J. Tromberg, "Increasing efficiency of two-photon excited fluorescence and second harmonic generation using ultrashort laser pulses," Proc. SPIE 6089, 308-315 (2006).

Tirlapur, U. K.

U. K. Tirlapur and K. König, "Femtosecond near-infrared laser pulses as versatile non-invasive tool for intratissue nanoprocessing in plants without compromising viability," The Plant Journal 31, 365-374 (2002).
[CrossRef]

Tirpalur, U. K.

U. K. Tirpalur and K. König, "Targeted transfection by femtosecond laser," Nature 418, 4295-4298 (2002).

Tolic-Norrelykke, I.

L. Sacconi, I. Tolic-Norrelykke, R. Antolini, and F. S. Pavone, "Combined intracellualar three-dimensional imaging and selective nanosurgery by a nonlinear microscope," J. Biomed. Opt. 10, 14002 (2005).
[CrossRef]

Tromberg, B. J.

S. Tang, T. B. Krasieva, Z. Chen, G. Tempea, and B. J. Tromberg, "Increasing efficiency of two-photon excited fluorescence and second harmonic generation using ultrashort laser pulses," Proc. SPIE 6089, 308-315 (2006).

Tsampoula, X.

X. Tsampoula, V. Garces-Chavez, M. Comrie, D. J. Stevenson, B. Agate, C. T. A. Brown, F. Gunn-Moore, and K. Dholakia, "Femtosecond cellular transfection using a nondiffracting light beam," Appl. Phys. Lett. 91, 053902 (2007).
[CrossRef]

D. Stevenson, B. Agate, X. Tsampoula, P. Fischer, C. T. A. Brown, W. Sibbett, A. Riches, F. Gunn-Moore, and K. Dholakia, "Femtosecond optical transfection of cells: viability and efficiency," Opt. Express 14, 7125-7133 (2006).
[CrossRef]

Tuan, R. S.

H. Haleem-Smith, A. Derfoul, C. Okafor, R. Tuli, D. Olsen, D. J. Hall, and R. S. Tuan, "Optimization of high-efficiency transfection of adult human mesenchymal stem cells in vitro," Mol. Biotech. 3, 09-19 (2005).
[CrossRef]

Tuli, R.

H. Haleem-Smith, A. Derfoul, C. Okafor, R. Tuli, D. Olsen, D. J. Hall, and R. S. Tuan, "Optimization of high-efficiency transfection of adult human mesenchymal stem cells in vitro," Mol. Biotech. 3, 09-19 (2005).
[CrossRef]

Venugopalan, V.

A. Vogel and V. Venugopalan, "Mechanism pulsed laser ablation of biological tissues," Chem Rev. 103, 577-644 (2003).
[CrossRef]

V. Venugopalan, A. Guerra, K. Nahen, and A. Vogel, "Role of laser-induced plasma formation in pulse cellular microsurgery and micromanipualtion," Phys. Rev. Lett. 88, 078103 (2002).
[CrossRef]

Vogel, A.

A. Vogel and V. Venugopalan, "Mechanism pulsed laser ablation of biological tissues," Chem Rev. 103, 577-644 (2003).
[CrossRef]

V. Venugopalan, A. Guerra, K. Nahen, and A. Vogel, "Role of laser-induced plasma formation in pulse cellular microsurgery and micromanipualtion," Phys. Rev. Lett. 88, 078103 (2002).
[CrossRef]

Watanabe, W.

W. Watanabe, S. Matsunaga, T. Shimada, T. Higashi, K. Fukui, and K. Itoh, "Femtosecond laser disruption of mitochondria in living cells," Med. Laser Appl. 20, 185-191 (2005).
[CrossRef]

Wedel, T.

C. Kruse, J. Kajahn, A. E. Petschnik, A. Maas, E. Klink, D. H. Rapoport, and T. Wedel, "Adult pancreatic stem/progenitor cells spontaneously differentiate in vitro into multiple cell lineages and form teratoma-like structures," Annals of Anatomy 188, 503-517 (2006).
[CrossRef]

C. Kruse, M. Birth, J. Rohwedel, K. Assmuth, A. Goepel, and T. Wedel, "Pluripotency of adult stem cells derived from human and rat pancreas," Appl. Phys. A. 79, 1617-1624 (2004).

Weise, A.

A. Czaki, F. Garwe, A. Steinbrueck, G. Maubach, G. Festag, A. Weise, I. Riemann, K. König, and W. Fritzsche, "A parallel approach for sub-wavelength molecular surgery using gene-specific positioned metal nanoparticles as laser light antennas," Nanoletters 2, 247-253 (2007).

Wolleschensky, R.

M. Müller, J. Squier, R. Wolleschensky, U. Simon, and J. Brakenhoff, "Dispersion pre compensation for high-numerical aperture objectives," J. Microsc. 191, 141-150 (1997).
[CrossRef]

Yanik, M. F.

M. F. Yanik, H. Cinar, H. N. Cinar, A. D. Chisholm, Y. Jin, and A. Ben-Yakar, "Nanosurgery: functional regeneration after laser axetomy," Nature 432, 822 (2004).
[CrossRef]

Yeh, A. T.

Annals of Anatomy (1)

C. Kruse, J. Kajahn, A. E. Petschnik, A. Maas, E. Klink, D. H. Rapoport, and T. Wedel, "Adult pancreatic stem/progenitor cells spontaneously differentiate in vitro into multiple cell lineages and form teratoma-like structures," Annals of Anatomy 188, 503-517 (2006).
[CrossRef]

Appl. Phys. A. (1)

C. Kruse, M. Birth, J. Rohwedel, K. Assmuth, A. Goepel, and T. Wedel, "Pluripotency of adult stem cells derived from human and rat pancreas," Appl. Phys. A. 79, 1617-1624 (2004).

Appl. Phys. B (1)

F. W. Helbing, G. Steinmeyer, J. Stenger, H. R. Telle, and U. Keller, "Carrier-envelope-offset dynamics and stabilization of femtosecond pulses," Appl. Phys. B 74, S35-S42 (2002).
[CrossRef]

Appl. Phys. Lett. (1)

X. Tsampoula, V. Garces-Chavez, M. Comrie, D. J. Stevenson, B. Agate, C. T. A. Brown, F. Gunn-Moore, and K. Dholakia, "Femtosecond cellular transfection using a nondiffracting light beam," Appl. Phys. Lett. 91, 053902 (2007).
[CrossRef]

Chem Rev. (1)

A. Vogel and V. Venugopalan, "Mechanism pulsed laser ablation of biological tissues," Chem Rev. 103, 577-644 (2003).
[CrossRef]

J. Biomed. Opt. (2)

G. McConnell and E. Riss, "Two-photon laser scanning microscopy using photonic crystal fiber," J. Biomed. Opt. 9, 922-927 (2004).
[CrossRef]

L. Sacconi, I. Tolic-Norrelykke, R. Antolini, and F. S. Pavone, "Combined intracellualar three-dimensional imaging and selective nanosurgery by a nonlinear microscope," J. Biomed. Opt. 10, 14002 (2005).
[CrossRef]

J. Microsc. (1)

M. Müller, J. Squier, R. Wolleschensky, U. Simon, and J. Brakenhoff, "Dispersion pre compensation for high-numerical aperture objectives," J. Microsc. 191, 141-150 (1997).
[CrossRef]

J. Photochem. Photobiol. B. (1)

F. Stracke, I. Riemann, and K. König, "Optical nanoinjection of macromolecules into vital cells," J. Photochem. Photobiol. B. 81, 136-142 (2005).
[CrossRef]

Lasers Surg. Med. (1)

V. Kohli, A. Elezzabi, and J. P. Acker, "Cell nanosurgery using ultrashort (femtosecond) laser pulses: applications to membrane surgery and cell isolation," Lasers Surg. Med. 37, 227-230 (2005).
[CrossRef]

Med. Laser Appl. (2)

W. Watanabe, S. Matsunaga, T. Shimada, T. Higashi, K. Fukui, and K. Itoh, "Femtosecond laser disruption of mitochondria in living cells," Med. Laser Appl. 20, 185-191 (2005).
[CrossRef]

K. König, I. Riemann, F. Stracke, and R. LeHarzic, "Nanoprocessing with nanojoule near-infrared femtosecond laser pulses," Med. Laser Appl. 20, 169-184 (2005).
[CrossRef]

Mol. Biotech. (1)

H. Haleem-Smith, A. Derfoul, C. Okafor, R. Tuli, D. Olsen, D. J. Hall, and R. S. Tuan, "Optimization of high-efficiency transfection of adult human mesenchymal stem cells in vitro," Mol. Biotech. 3, 09-19 (2005).
[CrossRef]

Nanoletters (1)

A. Czaki, F. Garwe, A. Steinbrueck, G. Maubach, G. Festag, A. Weise, I. Riemann, K. König, and W. Fritzsche, "A parallel approach for sub-wavelength molecular surgery using gene-specific positioned metal nanoparticles as laser light antennas," Nanoletters 2, 247-253 (2007).

Nature (2)

M. F. Yanik, H. Cinar, H. N. Cinar, A. D. Chisholm, Y. Jin, and A. Ben-Yakar, "Nanosurgery: functional regeneration after laser axetomy," Nature 432, 822 (2004).
[CrossRef]

U. K. Tirpalur and K. König, "Targeted transfection by femtosecond laser," Nature 418, 4295-4298 (2002).

Opt. Express (2)

Opt. Lett. (5)

Phys. Rev. Lett. (1)

V. Venugopalan, A. Guerra, K. Nahen, and A. Vogel, "Role of laser-induced plasma formation in pulse cellular microsurgery and micromanipualtion," Phys. Rev. Lett. 88, 078103 (2002).
[CrossRef]

Proc. SPIE (1)

S. Tang, T. B. Krasieva, Z. Chen, G. Tempea, and B. J. Tromberg, "Increasing efficiency of two-photon excited fluorescence and second harmonic generation using ultrashort laser pulses," Proc. SPIE 6089, 308-315 (2006).

The Plant Journal (1)

U. K. Tirlapur and K. König, "Femtosecond near-infrared laser pulses as versatile non-invasive tool for intratissue nanoprocessing in plants without compromising viability," The Plant Journal 31, 365-374 (2002).
[CrossRef]

Tissue Engineering (1)

A. Hamm, N. Krott, I. Breibach, R. Blindt, and A. K. Bosserhof, "Efficient transfection method for primary cells," Tissue Engineering 8, 1-13 (2002).
[CrossRef]

Other (1)

G. Tempea, B. Považay, A. Assion, A. Isemann, W. Pervak, M. Kempe, A. Stingl, and W. Drexler, "Undistored delivery of sub-15 fs pulses via high-numerical-aperture microscope objectives," Proc. SPIE 6442, 64420P-1-5 (2007).

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Metrics