Abstract

In this work, we present a method providing real-time, low cost, three-dimensional imaging in a three-dimensional optical micromanipulation system. The three-dimensional imaging system is based on a small form factor LED based projector. The projector is used to dynamically shape the rear illumination light in a counter-propagating beam-trapping setup. This allows us to produce stereoscopic images, from which the human brain can construct a three-dimensional image, or alternatively image analysis can be applied by a computer, thereby obtaining true three-dimensional coordinates in real-time for the trapped objects.

© 2008 Optical Society of America

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References

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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
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2007 (5)

2006 (3)

2005 (1)

2004 (1)

2003 (2)

K. Burton, "An aperture-shifting light-microscopic method for rapidly quantifying positions of cells in 3D matrices," Cytometry Part A 54, 125-131 (2003).

D. G. Grier, "A revolution in optical manipulation," Nature 424, 810-816 (2003).
[CrossRef]

1970 (1)

A. Ashkin, "Acceleration and trapping of particles by radiation pressure," Phys. Rev. Lett. 24, 156 (1970).
[CrossRef]

Alonzo, C. A.

Ashkin, A.

A. Ashkin, "Acceleration and trapping of particles by radiation pressure," Phys. Rev. Lett. 24, 156 (1970).
[CrossRef]

Blanca, C. M.

E. C. Samson and C. M. Blanca, "Dynamic contrast enhancement in widefield microscopy using projector-generated illumination patterns," New J. Phys. 9363 (2007).
[CrossRef]

S. Delica, and C. M. Blanca, "Wide-field depth-sectioning fluorescence microscopy using projector-generated patterned illumination," Appl. Opt. 46, 7237-7243 (2007).
[CrossRef]

Burton, K.

K. Burton, "An aperture-shifting light-microscopic method for rapidly quantifying positions of cells in 3D matrices," Cytometry Part A 54, 125-131 (2003).

Dam, J. S.

Daria, V. R.

Delica, S.

Glückstad, J.

Grier, D. G.

Isomura, A.

A. Isomura, N. Magome, M. I. Kohira, and K. Yoshikawa, "Toward the stable optical trapping of a droplet with counter laser beams under microgravity," Chem. Phys. Lett. 429, 321-325 (2006).
[CrossRef]

Kohira, M. I.

A. Isomura, N. Magome, M. I. Kohira, and K. Yoshikawa, "Toward the stable optical trapping of a droplet with counter laser beams under microgravity," Chem. Phys. Lett. 429, 321-325 (2006).
[CrossRef]

Lee, S. -H.

Magome, N.

A. Isomura, N. Magome, M. I. Kohira, and K. Yoshikawa, "Toward the stable optical trapping of a droplet with counter laser beams under microgravity," Chem. Phys. Lett. 429, 321-325 (2006).
[CrossRef]

Perch-Nielsen, I.

Perch-Nielsen, I. R.

Rodrigo, P.

Rodrigo, P. J.

Samson, E. C.

E. C. Samson and C. M. Blanca, "Dynamic contrast enhancement in widefield microscopy using projector-generated illumination patterns," New J. Phys. 9363 (2007).
[CrossRef]

Yoshikawa, K.

A. Isomura, N. Magome, M. I. Kohira, and K. Yoshikawa, "Toward the stable optical trapping of a droplet with counter laser beams under microgravity," Chem. Phys. Lett. 429, 321-325 (2006).
[CrossRef]

Appl. Opt. (1)

Chem. Phys. Lett. (1)

A. Isomura, N. Magome, M. I. Kohira, and K. Yoshikawa, "Toward the stable optical trapping of a droplet with counter laser beams under microgravity," Chem. Phys. Lett. 429, 321-325 (2006).
[CrossRef]

Cytometry Part A (1)

K. Burton, "An aperture-shifting light-microscopic method for rapidly quantifying positions of cells in 3D matrices," Cytometry Part A 54, 125-131 (2003).

Nature (1)

D. G. Grier, "A revolution in optical manipulation," Nature 424, 810-816 (2003).
[CrossRef]

New J. Phys. (1)

E. C. Samson and C. M. Blanca, "Dynamic contrast enhancement in widefield microscopy using projector-generated illumination patterns," New J. Phys. 9363 (2007).
[CrossRef]

Opt. Express (6)

Opt. Lett. (1)

Phys. Rev. Lett. (1)

A. Ashkin, "Acceleration and trapping of particles by radiation pressure," Phys. Rev. Lett. 24, 156 (1970).
[CrossRef]

Supplementary Material (2)

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