Abstract

We demonstrate a new method of measuring quantitative phase in imaging of biological materials. This method, asynchronous digital holography, employs knowledge of a moving fringe created by acousto-optic modulators to execute phase-shifting interferometry using two near-simultaneous interferograms. The method can be used to obtain quantitative phase images of dynamic biological samples on millisecond time scales. We present results on a standard sample, and on live cell samples.

© 2007 Optical Society of America

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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
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2006 (2)

2005 (3)

2000 (1)

L. Xu, J. Miao, and A. Asundi, "Properties of digital holography based on in-line configuration," Opt. Eng. 39, 3214 (2000).
[CrossRef]

1999 (1)

1998 (1)

1986 (1)

T. Kreis, "Digital Holographic Interference-Phase Measurement Using the Fourier-Transform Method," J. Opt. Soc. A 3, 847 (1986).

Asundi, A.

L. Xu, J. Miao, and A. Asundi, "Properties of digital holography based on in-line configuration," Opt. Eng. 39, 3214 (2000).
[CrossRef]

Badizadegan, K.

Bevilacqua, F.

Chicharo, J.

Colomb, T.

Cuche, E.

Dasari, R. R.

Depeursinge, C.

Emery, Y.

Feld, M. S.

Ikeda, T.

Kreis, T.

T. Kreis, "Digital Holographic Interference-Phase Measurement Using the Fourier-Transform Method," J. Opt. Soc. A 3, 847 (1986).

Li, E. B.

Lue, N.

Magistretti, P. J.

Marquet, P.

Miao, J.

L. Xu, J. Miao, and A. Asundi, "Properties of digital holography based on in-line configuration," Opt. Eng. 39, 3214 (2000).
[CrossRef]

Popescu, G.

Rappaz, B.

Xi, J. T.

Xu, D. Y.

Xu, L.

L. Xu, J. Miao, and A. Asundi, "Properties of digital holography based on in-line configuration," Opt. Eng. 39, 3214 (2000).
[CrossRef]

Yamaguchi, I.

Yao, J. Q.

Zhang, T.

J. Opt. Soc. A (1)

T. Kreis, "Digital Holographic Interference-Phase Measurement Using the Fourier-Transform Method," J. Opt. Soc. A 3, 847 (1986).

Opt. Eng. (1)

L. Xu, J. Miao, and A. Asundi, "Properties of digital holography based on in-line configuration," Opt. Eng. 39, 3214 (2000).
[CrossRef]

Opt. Lett. (7)

Other (4)

U. Schnars. and W. Jueptner, Digital holography : digital hologram recording, numerical reconstruction, and related techniques (Berlin, Springer, 2005).

S. L. Hahn, Hilbert transforms in signal processing (Artech House, Boston, 1996).

D. C. Ghiglia and M. D. Pritt, Two-dimensional phase unwrapping : theory, algorithms, and software (New York, Wiley, 1998).

T. Kreis, Holographic interferometry : principles and methods (Berlin: Akademie Verlag, 1996).

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