Abstract

Optical chromatography is a technique for the separation of particles that capitalizes on the balance between optic and fluidic forces. When microscopic particles in a fluid flow encounter a laser beam propagating in the opposite direction, they are trapped axially along the beam. They are then optically pushed upstream from the laser focal point to rest at a point where the optic and fluidic forces on the particle balance. Because optical and fluid forces are sensitive to differences in the physical and chemical properties of a particle, both coarse and fine separations are possible. We describe how an optical chromatography beam directed into a tailored flow environment, has been adapted to operate as an optical filter for the concentration/bioenrichment of colloidal and biological samples. In this work, the demonstrated ability to concentrate spores of the biowarfare agent, Bacillus anthracis, may have significant impact in the biodefense arena. Application of these techniques and further design of fluidic and optical environments will allow for more specific identification, concentration and separation of many more microscopic particle and biological suspensions.

© 2007 Optical Society of America

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  1. D. G. Grier, "A revolution in optical manipulation," Nature 424, 810-816 (2003).
    [CrossRef] [PubMed]
  2. A. Terray, J. Oakey, and D. W. M. Marr, "Fabrication of linear colloidal structures for microfluidic applications," Appl. Phys. Lett. 81, 1555-1557 (2002).
    [CrossRef]
  3. A. Ashkin, "History of optical trapping and manipulation of small-neutral particle, atoms, and molecules," IEEE J. Quantum Electron. 6, 841-856 (2000).
    [CrossRef]
  4. C. Mio, T. Gong, A. Terray, and D. W. M. Marr, "Design of a scanning laser optical trap for multiparticle manipulation," Rev. Sci. Instrum. 71, 2196-2200 (2000).
    [CrossRef]
  5. T. N. Buican, M. J. Smyth, H. A. Crissman, G. C. Salzman, C. C. Stewart, and J. C. Martin, "Automated Single-Cell Manipulation and Sorting by Light Trapping," Appl. Opt. 26, 5311-5316 (1987).
    [CrossRef] [PubMed]
  6. R. W. Applegate, J. Squier, T. Vestad, J. Oakey, D. W. M. Marr, P. Bado, M. A. Dugan, and A. A. Said, "Microfluidic sorting system based on optical waveguide integration and diode laser bar trapping," Lab on a Chip 6, 422-426 (2006).
    [CrossRef]
  7. K. Ladavak, K. Kasza, and D. Grier, "Sorting by Periodic Potential Energy Landscapes: Optical Fractionation," Phys. Rev. E 70, 010901 (2004).
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    [CrossRef] [PubMed]
  9. M. P. MacDonald, S. Neale, L. Paterson, A. Richies, K. Dholakia, and G. C. Spalding, "Cell cytometry with a light touch: Sorting microscopic matter with an optical lattice," J. Biol. Regul. Homeost. Agents 18, 200-205 (2004).
    [PubMed]
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    [CrossRef] [PubMed]
  11. D. McGloin, G. C. Spalding, H. Melville, W. Sibbett, and K. Dholakia, "Three-dimensional arrays of optical bottle beams," Opt. Commun. 225, 215-222 (2003).
    [CrossRef]
  12. T. Kaneta, Y. Ishidzu, N. Mishima, and T. Imasaka, "Theory of optical chromatography," Anal. Chem. 69, 2701-2710 (1997).
    [CrossRef] [PubMed]
  13. T. Imasaka, Y. Kawabata, T. Kaneta, and Y. Ishidzu, "Optical Chromatography," Anal. Chem. 67, 1763-1765 (1995).
    [CrossRef]
  14. T. Imasaka, "Optical chromatography. A new tool for separation of particles," Analusis 26, M53-M55 (1998).
    [CrossRef]
  15. A. Terray, J. Arnold, and S. J. Hart, "Enhanced optical chromatography in a PDMS microfluidic system," Opt. Express 13, 10406-10415 (2005).
    [CrossRef] [PubMed]
  16. S. J. Hart, and A. V. Terray, "Refractive-index-driven separation of colloidal polymer particles using optical chromatography," Appl. Phys. Lett. 83, 5316-5318 (2003).
    [CrossRef]
  17. J. Makihara, T. Kaneta, and T. Imasaka, "Optical chromatography: Size determination by eluting particles," Talanta 48, 551-557 (1999).
    [CrossRef]
  18. S. J. Hart, A. Terray, K. A. Kuhn, J. Arnold, and T. A. Leski, "Optical Chromatography of Biological Particles," Am. Lab. 36, 13-17 (2004).
  19. S. J. Hart, A. Terray, T. A. Leski, J. Arnold, and R. Stroud, "Discovery of a significant optical chromatographic difference between spores of Bacillus anthracis and its close relative, Bacillus thuringiensis," Anal. Chem. 78, 3221-3225 (2006).
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    [CrossRef]
  22. P. R. T. Jess, V. Garces-Chavez, D. Smith, M. Mazilu, L. Paterson, A. Riches, C. S. Herrington, W. Sibbett, and K. Dholakia, "Dual beam fibre trap for Raman microspectroscopy of single cells," Opt. Express 14, 5779-5791 (2006).
    [CrossRef] [PubMed]

2006 (3)

R. W. Applegate, J. Squier, T. Vestad, J. Oakey, D. W. M. Marr, P. Bado, M. A. Dugan, and A. A. Said, "Microfluidic sorting system based on optical waveguide integration and diode laser bar trapping," Lab on a Chip 6, 422-426 (2006).
[CrossRef]

S. J. Hart, A. Terray, T. A. Leski, J. Arnold, and R. Stroud, "Discovery of a significant optical chromatographic difference between spores of Bacillus anthracis and its close relative, Bacillus thuringiensis," Anal. Chem. 78, 3221-3225 (2006).
[CrossRef] [PubMed]

P. R. T. Jess, V. Garces-Chavez, D. Smith, M. Mazilu, L. Paterson, A. Riches, C. S. Herrington, W. Sibbett, and K. Dholakia, "Dual beam fibre trap for Raman microspectroscopy of single cells," Opt. Express 14, 5779-5791 (2006).
[CrossRef] [PubMed]

2005 (1)

2004 (4)

K. Ladavak, K. Kasza, and D. Grier, "Sorting by Periodic Potential Energy Landscapes: Optical Fractionation," Phys. Rev. E 70, 010901 (2004).

S. J. Hart, A. Terray, K. A. Kuhn, J. Arnold, and T. A. Leski, "Optical Chromatography of Biological Particles," Am. Lab. 36, 13-17 (2004).

M. P. MacDonald, S. Neale, L. Paterson, A. Richies, K. Dholakia, and G. C. Spalding, "Cell cytometry with a light touch: Sorting microscopic matter with an optical lattice," J. Biol. Regul. Homeost. Agents 18, 200-205 (2004).
[PubMed]

P. A. Prentice, M. P. MacDonald, T. G. Frank, A. Cuschieri, G. C. Spalding, W. Sibbett, P. A. Campbell, and K. Dholakia, "Manipulation and filtration of low index particles with holographic Laguerre-Gaussian optical trap arrays," Opt. Express 12, 593-600 (2004).
[CrossRef] [PubMed]

2003 (4)

D. McGloin, G. C. Spalding, H. Melville, W. Sibbett, and K. Dholakia, "Three-dimensional arrays of optical bottle beams," Opt. Commun. 225, 215-222 (2003).
[CrossRef]

D. G. Grier, "A revolution in optical manipulation," Nature 424, 810-816 (2003).
[CrossRef] [PubMed]

M. P. MacDonald, G. C. Spalding, and K. Dholakia, "Microfluidic sorting in an optical lattice," Nature 426, 421-424 (2003).
[CrossRef] [PubMed]

S. J. Hart, and A. V. Terray, "Refractive-index-driven separation of colloidal polymer particles using optical chromatography," Appl. Phys. Lett. 83, 5316-5318 (2003).
[CrossRef]

2002 (1)

A. Terray, J. Oakey, and D. W. M. Marr, "Fabrication of linear colloidal structures for microfluidic applications," Appl. Phys. Lett. 81, 1555-1557 (2002).
[CrossRef]

2000 (2)

A. Ashkin, "History of optical trapping and manipulation of small-neutral particle, atoms, and molecules," IEEE J. Quantum Electron. 6, 841-856 (2000).
[CrossRef]

C. Mio, T. Gong, A. Terray, and D. W. M. Marr, "Design of a scanning laser optical trap for multiparticle manipulation," Rev. Sci. Instrum. 71, 2196-2200 (2000).
[CrossRef]

1999 (1)

J. Makihara, T. Kaneta, and T. Imasaka, "Optical chromatography: Size determination by eluting particles," Talanta 48, 551-557 (1999).
[CrossRef]

1998 (1)

T. Imasaka, "Optical chromatography. A new tool for separation of particles," Analusis 26, M53-M55 (1998).
[CrossRef]

1997 (1)

T. Kaneta, Y. Ishidzu, N. Mishima, and T. Imasaka, "Theory of optical chromatography," Anal. Chem. 69, 2701-2710 (1997).
[CrossRef] [PubMed]

1996 (1)

N. Kitamura, M. Hayashi, H. B. Kim, and K. Nakatani, "Photometric analyses of optically-trapped single microparticles in solution," Anal. Sci. 12, 49-54 (1996).
[CrossRef]

1995 (1)

T. Imasaka, Y. Kawabata, T. Kaneta, and Y. Ishidzu, "Optical Chromatography," Anal. Chem. 67, 1763-1765 (1995).
[CrossRef]

1987 (1)

Applegate, R. W.

R. W. Applegate, J. Squier, T. Vestad, J. Oakey, D. W. M. Marr, P. Bado, M. A. Dugan, and A. A. Said, "Microfluidic sorting system based on optical waveguide integration and diode laser bar trapping," Lab on a Chip 6, 422-426 (2006).
[CrossRef]

Arnold, J.

S. J. Hart, A. Terray, T. A. Leski, J. Arnold, and R. Stroud, "Discovery of a significant optical chromatographic difference between spores of Bacillus anthracis and its close relative, Bacillus thuringiensis," Anal. Chem. 78, 3221-3225 (2006).
[CrossRef] [PubMed]

A. Terray, J. Arnold, and S. J. Hart, "Enhanced optical chromatography in a PDMS microfluidic system," Opt. Express 13, 10406-10415 (2005).
[CrossRef] [PubMed]

S. J. Hart, A. Terray, K. A. Kuhn, J. Arnold, and T. A. Leski, "Optical Chromatography of Biological Particles," Am. Lab. 36, 13-17 (2004).

Ashkin, A.

A. Ashkin, "History of optical trapping and manipulation of small-neutral particle, atoms, and molecules," IEEE J. Quantum Electron. 6, 841-856 (2000).
[CrossRef]

Bado, P.

R. W. Applegate, J. Squier, T. Vestad, J. Oakey, D. W. M. Marr, P. Bado, M. A. Dugan, and A. A. Said, "Microfluidic sorting system based on optical waveguide integration and diode laser bar trapping," Lab on a Chip 6, 422-426 (2006).
[CrossRef]

Buican, T. N.

Campbell, P. A.

Crissman, H. A.

Cuschieri, A.

Dholakia, K.

P. R. T. Jess, V. Garces-Chavez, D. Smith, M. Mazilu, L. Paterson, A. Riches, C. S. Herrington, W. Sibbett, and K. Dholakia, "Dual beam fibre trap for Raman microspectroscopy of single cells," Opt. Express 14, 5779-5791 (2006).
[CrossRef] [PubMed]

P. A. Prentice, M. P. MacDonald, T. G. Frank, A. Cuschieri, G. C. Spalding, W. Sibbett, P. A. Campbell, and K. Dholakia, "Manipulation and filtration of low index particles with holographic Laguerre-Gaussian optical trap arrays," Opt. Express 12, 593-600 (2004).
[CrossRef] [PubMed]

M. P. MacDonald, S. Neale, L. Paterson, A. Richies, K. Dholakia, and G. C. Spalding, "Cell cytometry with a light touch: Sorting microscopic matter with an optical lattice," J. Biol. Regul. Homeost. Agents 18, 200-205 (2004).
[PubMed]

M. P. MacDonald, G. C. Spalding, and K. Dholakia, "Microfluidic sorting in an optical lattice," Nature 426, 421-424 (2003).
[CrossRef] [PubMed]

D. McGloin, G. C. Spalding, H. Melville, W. Sibbett, and K. Dholakia, "Three-dimensional arrays of optical bottle beams," Opt. Commun. 225, 215-222 (2003).
[CrossRef]

Dugan, M. A.

R. W. Applegate, J. Squier, T. Vestad, J. Oakey, D. W. M. Marr, P. Bado, M. A. Dugan, and A. A. Said, "Microfluidic sorting system based on optical waveguide integration and diode laser bar trapping," Lab on a Chip 6, 422-426 (2006).
[CrossRef]

Frank, T. G.

Garces-Chavez, V.

Gong, T.

C. Mio, T. Gong, A. Terray, and D. W. M. Marr, "Design of a scanning laser optical trap for multiparticle manipulation," Rev. Sci. Instrum. 71, 2196-2200 (2000).
[CrossRef]

Grier, D.

K. Ladavak, K. Kasza, and D. Grier, "Sorting by Periodic Potential Energy Landscapes: Optical Fractionation," Phys. Rev. E 70, 010901 (2004).

Grier, D. G.

D. G. Grier, "A revolution in optical manipulation," Nature 424, 810-816 (2003).
[CrossRef] [PubMed]

Hart, S. J.

S. J. Hart, A. Terray, T. A. Leski, J. Arnold, and R. Stroud, "Discovery of a significant optical chromatographic difference between spores of Bacillus anthracis and its close relative, Bacillus thuringiensis," Anal. Chem. 78, 3221-3225 (2006).
[CrossRef] [PubMed]

A. Terray, J. Arnold, and S. J. Hart, "Enhanced optical chromatography in a PDMS microfluidic system," Opt. Express 13, 10406-10415 (2005).
[CrossRef] [PubMed]

S. J. Hart, A. Terray, K. A. Kuhn, J. Arnold, and T. A. Leski, "Optical Chromatography of Biological Particles," Am. Lab. 36, 13-17 (2004).

S. J. Hart, and A. V. Terray, "Refractive-index-driven separation of colloidal polymer particles using optical chromatography," Appl. Phys. Lett. 83, 5316-5318 (2003).
[CrossRef]

Hayashi, M.

N. Kitamura, M. Hayashi, H. B. Kim, and K. Nakatani, "Photometric analyses of optically-trapped single microparticles in solution," Anal. Sci. 12, 49-54 (1996).
[CrossRef]

Herrington, C. S.

Imasaka, T.

J. Makihara, T. Kaneta, and T. Imasaka, "Optical chromatography: Size determination by eluting particles," Talanta 48, 551-557 (1999).
[CrossRef]

T. Imasaka, "Optical chromatography. A new tool for separation of particles," Analusis 26, M53-M55 (1998).
[CrossRef]

T. Kaneta, Y. Ishidzu, N. Mishima, and T. Imasaka, "Theory of optical chromatography," Anal. Chem. 69, 2701-2710 (1997).
[CrossRef] [PubMed]

T. Imasaka, Y. Kawabata, T. Kaneta, and Y. Ishidzu, "Optical Chromatography," Anal. Chem. 67, 1763-1765 (1995).
[CrossRef]

Ishidzu, Y.

T. Kaneta, Y. Ishidzu, N. Mishima, and T. Imasaka, "Theory of optical chromatography," Anal. Chem. 69, 2701-2710 (1997).
[CrossRef] [PubMed]

T. Imasaka, Y. Kawabata, T. Kaneta, and Y. Ishidzu, "Optical Chromatography," Anal. Chem. 67, 1763-1765 (1995).
[CrossRef]

Jess, P. R. T.

Kaneta, T.

J. Makihara, T. Kaneta, and T. Imasaka, "Optical chromatography: Size determination by eluting particles," Talanta 48, 551-557 (1999).
[CrossRef]

T. Kaneta, Y. Ishidzu, N. Mishima, and T. Imasaka, "Theory of optical chromatography," Anal. Chem. 69, 2701-2710 (1997).
[CrossRef] [PubMed]

T. Imasaka, Y. Kawabata, T. Kaneta, and Y. Ishidzu, "Optical Chromatography," Anal. Chem. 67, 1763-1765 (1995).
[CrossRef]

Kasza, K.

K. Ladavak, K. Kasza, and D. Grier, "Sorting by Periodic Potential Energy Landscapes: Optical Fractionation," Phys. Rev. E 70, 010901 (2004).

Kawabata, Y.

T. Imasaka, Y. Kawabata, T. Kaneta, and Y. Ishidzu, "Optical Chromatography," Anal. Chem. 67, 1763-1765 (1995).
[CrossRef]

Kim, H. B.

N. Kitamura, M. Hayashi, H. B. Kim, and K. Nakatani, "Photometric analyses of optically-trapped single microparticles in solution," Anal. Sci. 12, 49-54 (1996).
[CrossRef]

Kitamura, N.

N. Kitamura, M. Hayashi, H. B. Kim, and K. Nakatani, "Photometric analyses of optically-trapped single microparticles in solution," Anal. Sci. 12, 49-54 (1996).
[CrossRef]

Kuhn, K. A.

S. J. Hart, A. Terray, K. A. Kuhn, J. Arnold, and T. A. Leski, "Optical Chromatography of Biological Particles," Am. Lab. 36, 13-17 (2004).

Ladavak, K.

K. Ladavak, K. Kasza, and D. Grier, "Sorting by Periodic Potential Energy Landscapes: Optical Fractionation," Phys. Rev. E 70, 010901 (2004).

Leski, T. A.

S. J. Hart, A. Terray, T. A. Leski, J. Arnold, and R. Stroud, "Discovery of a significant optical chromatographic difference between spores of Bacillus anthracis and its close relative, Bacillus thuringiensis," Anal. Chem. 78, 3221-3225 (2006).
[CrossRef] [PubMed]

S. J. Hart, A. Terray, K. A. Kuhn, J. Arnold, and T. A. Leski, "Optical Chromatography of Biological Particles," Am. Lab. 36, 13-17 (2004).

MacDonald, M. P.

M. P. MacDonald, S. Neale, L. Paterson, A. Richies, K. Dholakia, and G. C. Spalding, "Cell cytometry with a light touch: Sorting microscopic matter with an optical lattice," J. Biol. Regul. Homeost. Agents 18, 200-205 (2004).
[PubMed]

P. A. Prentice, M. P. MacDonald, T. G. Frank, A. Cuschieri, G. C. Spalding, W. Sibbett, P. A. Campbell, and K. Dholakia, "Manipulation and filtration of low index particles with holographic Laguerre-Gaussian optical trap arrays," Opt. Express 12, 593-600 (2004).
[CrossRef] [PubMed]

M. P. MacDonald, G. C. Spalding, and K. Dholakia, "Microfluidic sorting in an optical lattice," Nature 426, 421-424 (2003).
[CrossRef] [PubMed]

Makihara, J.

J. Makihara, T. Kaneta, and T. Imasaka, "Optical chromatography: Size determination by eluting particles," Talanta 48, 551-557 (1999).
[CrossRef]

Marr, D. W. M.

R. W. Applegate, J. Squier, T. Vestad, J. Oakey, D. W. M. Marr, P. Bado, M. A. Dugan, and A. A. Said, "Microfluidic sorting system based on optical waveguide integration and diode laser bar trapping," Lab on a Chip 6, 422-426 (2006).
[CrossRef]

A. Terray, J. Oakey, and D. W. M. Marr, "Fabrication of linear colloidal structures for microfluidic applications," Appl. Phys. Lett. 81, 1555-1557 (2002).
[CrossRef]

C. Mio, T. Gong, A. Terray, and D. W. M. Marr, "Design of a scanning laser optical trap for multiparticle manipulation," Rev. Sci. Instrum. 71, 2196-2200 (2000).
[CrossRef]

Martin, J. C.

Mazilu, M.

McGloin, D.

D. McGloin, G. C. Spalding, H. Melville, W. Sibbett, and K. Dholakia, "Three-dimensional arrays of optical bottle beams," Opt. Commun. 225, 215-222 (2003).
[CrossRef]

Melville, H.

D. McGloin, G. C. Spalding, H. Melville, W. Sibbett, and K. Dholakia, "Three-dimensional arrays of optical bottle beams," Opt. Commun. 225, 215-222 (2003).
[CrossRef]

Mio, C.

C. Mio, T. Gong, A. Terray, and D. W. M. Marr, "Design of a scanning laser optical trap for multiparticle manipulation," Rev. Sci. Instrum. 71, 2196-2200 (2000).
[CrossRef]

Mishima, N.

T. Kaneta, Y. Ishidzu, N. Mishima, and T. Imasaka, "Theory of optical chromatography," Anal. Chem. 69, 2701-2710 (1997).
[CrossRef] [PubMed]

Nakatani, K.

N. Kitamura, M. Hayashi, H. B. Kim, and K. Nakatani, "Photometric analyses of optically-trapped single microparticles in solution," Anal. Sci. 12, 49-54 (1996).
[CrossRef]

Neale, S.

M. P. MacDonald, S. Neale, L. Paterson, A. Richies, K. Dholakia, and G. C. Spalding, "Cell cytometry with a light touch: Sorting microscopic matter with an optical lattice," J. Biol. Regul. Homeost. Agents 18, 200-205 (2004).
[PubMed]

Oakey, J.

R. W. Applegate, J. Squier, T. Vestad, J. Oakey, D. W. M. Marr, P. Bado, M. A. Dugan, and A. A. Said, "Microfluidic sorting system based on optical waveguide integration and diode laser bar trapping," Lab on a Chip 6, 422-426 (2006).
[CrossRef]

A. Terray, J. Oakey, and D. W. M. Marr, "Fabrication of linear colloidal structures for microfluidic applications," Appl. Phys. Lett. 81, 1555-1557 (2002).
[CrossRef]

Paterson, L.

P. R. T. Jess, V. Garces-Chavez, D. Smith, M. Mazilu, L. Paterson, A. Riches, C. S. Herrington, W. Sibbett, and K. Dholakia, "Dual beam fibre trap for Raman microspectroscopy of single cells," Opt. Express 14, 5779-5791 (2006).
[CrossRef] [PubMed]

M. P. MacDonald, S. Neale, L. Paterson, A. Richies, K. Dholakia, and G. C. Spalding, "Cell cytometry with a light touch: Sorting microscopic matter with an optical lattice," J. Biol. Regul. Homeost. Agents 18, 200-205 (2004).
[PubMed]

Prentice, P. A.

Riches, A.

Richies, A.

M. P. MacDonald, S. Neale, L. Paterson, A. Richies, K. Dholakia, and G. C. Spalding, "Cell cytometry with a light touch: Sorting microscopic matter with an optical lattice," J. Biol. Regul. Homeost. Agents 18, 200-205 (2004).
[PubMed]

Said, A. A.

R. W. Applegate, J. Squier, T. Vestad, J. Oakey, D. W. M. Marr, P. Bado, M. A. Dugan, and A. A. Said, "Microfluidic sorting system based on optical waveguide integration and diode laser bar trapping," Lab on a Chip 6, 422-426 (2006).
[CrossRef]

Salzman, G. C.

Sibbett, W.

Smith, D.

Smyth, M. J.

Spalding, G. C.

M. P. MacDonald, S. Neale, L. Paterson, A. Richies, K. Dholakia, and G. C. Spalding, "Cell cytometry with a light touch: Sorting microscopic matter with an optical lattice," J. Biol. Regul. Homeost. Agents 18, 200-205 (2004).
[PubMed]

P. A. Prentice, M. P. MacDonald, T. G. Frank, A. Cuschieri, G. C. Spalding, W. Sibbett, P. A. Campbell, and K. Dholakia, "Manipulation and filtration of low index particles with holographic Laguerre-Gaussian optical trap arrays," Opt. Express 12, 593-600 (2004).
[CrossRef] [PubMed]

D. McGloin, G. C. Spalding, H. Melville, W. Sibbett, and K. Dholakia, "Three-dimensional arrays of optical bottle beams," Opt. Commun. 225, 215-222 (2003).
[CrossRef]

M. P. MacDonald, G. C. Spalding, and K. Dholakia, "Microfluidic sorting in an optical lattice," Nature 426, 421-424 (2003).
[CrossRef] [PubMed]

Squier, J.

R. W. Applegate, J. Squier, T. Vestad, J. Oakey, D. W. M. Marr, P. Bado, M. A. Dugan, and A. A. Said, "Microfluidic sorting system based on optical waveguide integration and diode laser bar trapping," Lab on a Chip 6, 422-426 (2006).
[CrossRef]

Stewart, C. C.

Stroud, R.

S. J. Hart, A. Terray, T. A. Leski, J. Arnold, and R. Stroud, "Discovery of a significant optical chromatographic difference between spores of Bacillus anthracis and its close relative, Bacillus thuringiensis," Anal. Chem. 78, 3221-3225 (2006).
[CrossRef] [PubMed]

Terray, A.

S. J. Hart, A. Terray, T. A. Leski, J. Arnold, and R. Stroud, "Discovery of a significant optical chromatographic difference between spores of Bacillus anthracis and its close relative, Bacillus thuringiensis," Anal. Chem. 78, 3221-3225 (2006).
[CrossRef] [PubMed]

A. Terray, J. Arnold, and S. J. Hart, "Enhanced optical chromatography in a PDMS microfluidic system," Opt. Express 13, 10406-10415 (2005).
[CrossRef] [PubMed]

S. J. Hart, A. Terray, K. A. Kuhn, J. Arnold, and T. A. Leski, "Optical Chromatography of Biological Particles," Am. Lab. 36, 13-17 (2004).

A. Terray, J. Oakey, and D. W. M. Marr, "Fabrication of linear colloidal structures for microfluidic applications," Appl. Phys. Lett. 81, 1555-1557 (2002).
[CrossRef]

C. Mio, T. Gong, A. Terray, and D. W. M. Marr, "Design of a scanning laser optical trap for multiparticle manipulation," Rev. Sci. Instrum. 71, 2196-2200 (2000).
[CrossRef]

Terray, A. V.

S. J. Hart, and A. V. Terray, "Refractive-index-driven separation of colloidal polymer particles using optical chromatography," Appl. Phys. Lett. 83, 5316-5318 (2003).
[CrossRef]

Vestad, T.

R. W. Applegate, J. Squier, T. Vestad, J. Oakey, D. W. M. Marr, P. Bado, M. A. Dugan, and A. A. Said, "Microfluidic sorting system based on optical waveguide integration and diode laser bar trapping," Lab on a Chip 6, 422-426 (2006).
[CrossRef]

Am. Lab. (1)

S. J. Hart, A. Terray, K. A. Kuhn, J. Arnold, and T. A. Leski, "Optical Chromatography of Biological Particles," Am. Lab. 36, 13-17 (2004).

Anal. Chem. (3)

S. J. Hart, A. Terray, T. A. Leski, J. Arnold, and R. Stroud, "Discovery of a significant optical chromatographic difference between spores of Bacillus anthracis and its close relative, Bacillus thuringiensis," Anal. Chem. 78, 3221-3225 (2006).
[CrossRef] [PubMed]

T. Kaneta, Y. Ishidzu, N. Mishima, and T. Imasaka, "Theory of optical chromatography," Anal. Chem. 69, 2701-2710 (1997).
[CrossRef] [PubMed]

T. Imasaka, Y. Kawabata, T. Kaneta, and Y. Ishidzu, "Optical Chromatography," Anal. Chem. 67, 1763-1765 (1995).
[CrossRef]

Anal. Sci. (1)

N. Kitamura, M. Hayashi, H. B. Kim, and K. Nakatani, "Photometric analyses of optically-trapped single microparticles in solution," Anal. Sci. 12, 49-54 (1996).
[CrossRef]

Analusis (1)

T. Imasaka, "Optical chromatography. A new tool for separation of particles," Analusis 26, M53-M55 (1998).
[CrossRef]

Appl. Opt. (1)

Appl. Phys. Lett. (2)

A. Terray, J. Oakey, and D. W. M. Marr, "Fabrication of linear colloidal structures for microfluidic applications," Appl. Phys. Lett. 81, 1555-1557 (2002).
[CrossRef]

S. J. Hart, and A. V. Terray, "Refractive-index-driven separation of colloidal polymer particles using optical chromatography," Appl. Phys. Lett. 83, 5316-5318 (2003).
[CrossRef]

IEEE J. Quantum Electron. (1)

A. Ashkin, "History of optical trapping and manipulation of small-neutral particle, atoms, and molecules," IEEE J. Quantum Electron. 6, 841-856 (2000).
[CrossRef]

J. Biol. Regul. Homeost. Agents (1)

M. P. MacDonald, S. Neale, L. Paterson, A. Richies, K. Dholakia, and G. C. Spalding, "Cell cytometry with a light touch: Sorting microscopic matter with an optical lattice," J. Biol. Regul. Homeost. Agents 18, 200-205 (2004).
[PubMed]

Lab on a Chip (1)

R. W. Applegate, J. Squier, T. Vestad, J. Oakey, D. W. M. Marr, P. Bado, M. A. Dugan, and A. A. Said, "Microfluidic sorting system based on optical waveguide integration and diode laser bar trapping," Lab on a Chip 6, 422-426 (2006).
[CrossRef]

Nature (2)

D. G. Grier, "A revolution in optical manipulation," Nature 424, 810-816 (2003).
[CrossRef] [PubMed]

M. P. MacDonald, G. C. Spalding, and K. Dholakia, "Microfluidic sorting in an optical lattice," Nature 426, 421-424 (2003).
[CrossRef] [PubMed]

Opt. Commun. (1)

D. McGloin, G. C. Spalding, H. Melville, W. Sibbett, and K. Dholakia, "Three-dimensional arrays of optical bottle beams," Opt. Commun. 225, 215-222 (2003).
[CrossRef]

Opt. Express (3)

Phys. Rev. E (1)

K. Ladavak, K. Kasza, and D. Grier, "Sorting by Periodic Potential Energy Landscapes: Optical Fractionation," Phys. Rev. E 70, 010901 (2004).

Rev. Sci. Instrum. (1)

C. Mio, T. Gong, A. Terray, and D. W. M. Marr, "Design of a scanning laser optical trap for multiparticle manipulation," Rev. Sci. Instrum. 71, 2196-2200 (2000).
[CrossRef]

Talanta (1)

J. Makihara, T. Kaneta, and T. Imasaka, "Optical chromatography: Size determination by eluting particles," Talanta 48, 551-557 (1999).
[CrossRef]

Other (1)

S. J. Hart, "Theoretical Calculations and Estimates of Optical Separation of Particles Based Upon Chemical Composition," Naval Research Laboratory Memorandum Report NRL/MR/6110--01-8555 (2001).

Supplementary Material (2)

» Media 1: MOV (2327 KB)     
» Media 2: MOV (1396 KB)     

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Figures (5)

Fig.1.
Fig.1.

Optical chromatography glass micro-flowcell. A) Construction of the microfluidic device showing the pathway for fluid (shown in gray) into and out of the separation channel, and the laser beam focused through the channel. B) Illustration of a separation in which sample particles are constrained to the focal point of the beam by the size of the separation channel that is filled by the laser beam.

Fig. 2.
Fig. 2.

A) Stock concentration stream of 2 μm polymer particles in the channel, B) laser ON and retaining all polymer particles (no particles in the channel), C) concentrated stream of 2 μm polymer particles released when the laser was switched off. Laser power was 0.9 W at 1064 nm, fluid flow rate was 3.0 μL/hr.

Fig. 3.
Fig. 3.

Six minute laser concentration of 2 Fig. 3 μm polystyrene particles. A) Sample of polymer particles in system with laser off, B) laser ON – flowcell is free of polymer particles with the laser retaining them, C) laser OFF – flowcell is filled with concentrated stream of particles previously retained by the laser, D) return to original dilute mixture of polymer particles. Data were background subtracted.

Fig. 4.
Fig. 4.

Laser concentration of 2 micron PS particles. A) Baseline / no laser, B) 2 minute laser concentration of sample, C) 4 minutes of laser concentration, D) 6 minutes of laser concentration, E) 8 minutes of laser concentration, and F) 10 minutes of laser concentration.Images were taken from the peak region for each laser concentration time. (Supplemental Movie 1) [Media 1]

Fig. 5.
Fig. 5.

Injection of (□) B. anthracis spores in water with the laser off, and (◯) B.anthracis spores with the laser on. The laser beam was blocked at 10 min, releasing a concentrated band of B.a. spores. (Supplemental Movie 2) [Media 2]

Tables (1)

Tables Icon

Table 1. Time based laser concentration of particles

Metrics