Abstract

We developed a compact, high-peak-power picosecond pulse source based on a gain-switched laser diode with an emission wavelength of 980 nm and successfully demonstrated two-photon fluorescence imaging. The pulse source consisted of the gain-switched laser diode, a pulse compressor, a pulse reshaper, and optical amplifiers, all connected by single-mode fibers. The optical pulse generated by the pulse source had a pulse width of 3.5 ps, an average power of 35 mW, and kilowatt-level peak power at a repetition rate of 10 MHz. Alexa488-stained Glomeruli and convoluted tubules of a mouse kidney section were observed by two-photon imaging with the developed pulse source.

© 2007 Optical Society of America

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  1. W. Denk, J. H. Strickler, and W. W. Webb, "Two-photon laser scanning fluorescence microscopy," Science 248, 73-76 (1990).
    [CrossRef] [PubMed]
  2. W. R. Zipfel, R. M. Williams, and W. W. Webb, "Nonlinear magic: multiphoton microscopy in the biosciences," Nat. Biotechnol. 21, 1369-1377 (2003).
    [CrossRef] [PubMed]
  3. J. Squier and M. Müller, "High resolution nonlinear microscopy: A review of sources and methods for achieving optimal imaging," Rev. Sci. Inst. 72, 2855-2867 (2001).
    [CrossRef]
  4. J. M. Girkin and G. McConnell, "Advances in laser sources for confocal and multiphoton microscopy," Microsc. Res. Tech. 67, 8-14 (2005).
    [CrossRef] [PubMed]
  5. H. Yokoyama, H. Guo, T. Yoda, K. Takashima, K. Sato, H. Taniguchi, and H. Ito, "Two-photon bioimaging with picosecond optical pulses from a semiconductor laser," Opt. Express 14, 3467-3471 (2006), http://www.opticsinfobase.org/abstract.cfm?URI=oe-14-8-3467.
    [CrossRef] [PubMed]
  6. A. Takada, T. Sugie, and M. Saruwatari, "High-speed picosecond optical pulse compression from gain-switched 1.3-μm distributed feedback-laser diode (DFB-LD) through highly dispersive single-mode fiber," J. Lightwave Technol. 5, 1525-1533 (1987).
    [CrossRef]
  7. H. Guo, K. Sato, K. Takashima, and H. Yokoyama, "Two-photon bio-imaging with a mode-locked semiconductor laser," 15th International Conference on Ultrafast Phenomena, California, USA, TuE8, July 30 - August 4 (2006).

2006

2005

J. M. Girkin and G. McConnell, "Advances in laser sources for confocal and multiphoton microscopy," Microsc. Res. Tech. 67, 8-14 (2005).
[CrossRef] [PubMed]

2003

W. R. Zipfel, R. M. Williams, and W. W. Webb, "Nonlinear magic: multiphoton microscopy in the biosciences," Nat. Biotechnol. 21, 1369-1377 (2003).
[CrossRef] [PubMed]

2001

J. Squier and M. Müller, "High resolution nonlinear microscopy: A review of sources and methods for achieving optimal imaging," Rev. Sci. Inst. 72, 2855-2867 (2001).
[CrossRef]

1990

W. Denk, J. H. Strickler, and W. W. Webb, "Two-photon laser scanning fluorescence microscopy," Science 248, 73-76 (1990).
[CrossRef] [PubMed]

1987

A. Takada, T. Sugie, and M. Saruwatari, "High-speed picosecond optical pulse compression from gain-switched 1.3-μm distributed feedback-laser diode (DFB-LD) through highly dispersive single-mode fiber," J. Lightwave Technol. 5, 1525-1533 (1987).
[CrossRef]

Denk, W.

W. Denk, J. H. Strickler, and W. W. Webb, "Two-photon laser scanning fluorescence microscopy," Science 248, 73-76 (1990).
[CrossRef] [PubMed]

Girkin, J. M.

J. M. Girkin and G. McConnell, "Advances in laser sources for confocal and multiphoton microscopy," Microsc. Res. Tech. 67, 8-14 (2005).
[CrossRef] [PubMed]

Guo, H.

Ito, H.

McConnell, G.

J. M. Girkin and G. McConnell, "Advances in laser sources for confocal and multiphoton microscopy," Microsc. Res. Tech. 67, 8-14 (2005).
[CrossRef] [PubMed]

Müller, M.

J. Squier and M. Müller, "High resolution nonlinear microscopy: A review of sources and methods for achieving optimal imaging," Rev. Sci. Inst. 72, 2855-2867 (2001).
[CrossRef]

Saruwatari, M.

A. Takada, T. Sugie, and M. Saruwatari, "High-speed picosecond optical pulse compression from gain-switched 1.3-μm distributed feedback-laser diode (DFB-LD) through highly dispersive single-mode fiber," J. Lightwave Technol. 5, 1525-1533 (1987).
[CrossRef]

Sato, K.

Squier, J.

J. Squier and M. Müller, "High resolution nonlinear microscopy: A review of sources and methods for achieving optimal imaging," Rev. Sci. Inst. 72, 2855-2867 (2001).
[CrossRef]

Strickler, J. H.

W. Denk, J. H. Strickler, and W. W. Webb, "Two-photon laser scanning fluorescence microscopy," Science 248, 73-76 (1990).
[CrossRef] [PubMed]

Sugie, T.

A. Takada, T. Sugie, and M. Saruwatari, "High-speed picosecond optical pulse compression from gain-switched 1.3-μm distributed feedback-laser diode (DFB-LD) through highly dispersive single-mode fiber," J. Lightwave Technol. 5, 1525-1533 (1987).
[CrossRef]

Takada, A.

A. Takada, T. Sugie, and M. Saruwatari, "High-speed picosecond optical pulse compression from gain-switched 1.3-μm distributed feedback-laser diode (DFB-LD) through highly dispersive single-mode fiber," J. Lightwave Technol. 5, 1525-1533 (1987).
[CrossRef]

Takashima, K.

Taniguchi, H.

Webb, W. W.

W. R. Zipfel, R. M. Williams, and W. W. Webb, "Nonlinear magic: multiphoton microscopy in the biosciences," Nat. Biotechnol. 21, 1369-1377 (2003).
[CrossRef] [PubMed]

W. Denk, J. H. Strickler, and W. W. Webb, "Two-photon laser scanning fluorescence microscopy," Science 248, 73-76 (1990).
[CrossRef] [PubMed]

Williams, R. M.

W. R. Zipfel, R. M. Williams, and W. W. Webb, "Nonlinear magic: multiphoton microscopy in the biosciences," Nat. Biotechnol. 21, 1369-1377 (2003).
[CrossRef] [PubMed]

Yoda, T.

Yokoyama, H.

Zipfel, W. R.

W. R. Zipfel, R. M. Williams, and W. W. Webb, "Nonlinear magic: multiphoton microscopy in the biosciences," Nat. Biotechnol. 21, 1369-1377 (2003).
[CrossRef] [PubMed]

J. Lightwave Technol.

A. Takada, T. Sugie, and M. Saruwatari, "High-speed picosecond optical pulse compression from gain-switched 1.3-μm distributed feedback-laser diode (DFB-LD) through highly dispersive single-mode fiber," J. Lightwave Technol. 5, 1525-1533 (1987).
[CrossRef]

Microsc. Res. Tech.

J. M. Girkin and G. McConnell, "Advances in laser sources for confocal and multiphoton microscopy," Microsc. Res. Tech. 67, 8-14 (2005).
[CrossRef] [PubMed]

Nat. Biotechnol.

W. R. Zipfel, R. M. Williams, and W. W. Webb, "Nonlinear magic: multiphoton microscopy in the biosciences," Nat. Biotechnol. 21, 1369-1377 (2003).
[CrossRef] [PubMed]

Opt. Express

Rev. Sci. Inst.

J. Squier and M. Müller, "High resolution nonlinear microscopy: A review of sources and methods for achieving optimal imaging," Rev. Sci. Inst. 72, 2855-2867 (2001).
[CrossRef]

Science

W. Denk, J. H. Strickler, and W. W. Webb, "Two-photon laser scanning fluorescence microscopy," Science 248, 73-76 (1990).
[CrossRef] [PubMed]

Other

H. Guo, K. Sato, K. Takashima, and H. Yokoyama, "Two-photon bio-imaging with a mode-locked semiconductor laser," 15th International Conference on Ultrafast Phenomena, California, USA, TuE8, July 30 - August 4 (2006).

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Figures (4)

Fig. 1.
Fig. 1.

Pulse source setup. VCSEL: vertical cavity surface emitting laser, SOA: semiconductor optical amplifier, BPF: band-pass filter, YDFA: Yb-doped fiber amplifier.

Fig. 2.
Fig. 2.

Intensity auto-correlation trace (a) and optical spectrum (b).

Fig. 3.
Fig. 3.

Two-photon fluorescence imaging setup. PMT: photomultiplier tube. NA of the objective lens was 1.2 with water immersion.

Fig. 4.
Fig. 4.

Two-photon fluorescence imaging of a mouse kidney stained with AlexaFluor488.

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