We present a new imaging method to record multicolor digital holograms from
objects emitting fluorescent light. The fluorescent light specific to the
emission wavelength of various fluorescent dyes after excitation of three
dimensional (3D) objects is recorded on a digital monochrome camera after
reflection from a diffractive optical element (DOE). For each wavelength of
fluorescent emission, the camera sequentially records three holograms reflected
from the DOE, each with a different phase factor of the DOE’s
function. The three holograms are superposed in a computer to create a complex
valued Fresnel hologram of each fluorescent emission. The holograms for each
fluorescent color are further combined in a computer to produce a multicolored
fluorescence hologram and 3D color image.
©2007 Optical Society of America
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