Abstract

We describe an optical sectioning microscopy system with no moving parts based on a micro-structured stripe-array light emitting diode (LED). By projecting arbitrary line or grid patterns onto the object, we are able to implement a variety of optical sectioning microscopy techniques such as grid-projection structured illumination and line scanning confocal microscopy, switching from one imaging technique to another without modifying the microscope setup. The micro-structured LED and driver are detailed and depth discrimination capabilities are measured and calculated.

© 2007 Optical Society of America

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  1. T. Wilson and Barry R. Masters, Confocal Microscopy, (Academic Press, San Diego, 1990).
  2. W. B. Amos and J. G. White, "Direct view confocal imaging systems using a slit aperture," in Handbook of Biological Confocal Microscopy, J. B. Pawley (Plenum, New York, 1995), pp. 403-415.
  3. C. J. R. Sheppard and X. Q. Mao, "Confocal microscopes with slit apertures," J. Mod. Opt. 25, 1169-1185 (1988).
  4. M. A. A. Neil, R. Juskaitis, and T. Wilson, "Method of obtaining optical sectioning by using structured light in a conventional microscope," Opt. Lett. 22, 1905-1907 (1997).
  5. OptiGrid, QiOptiq Imaging Solutions, http://www.qioptiqimaging.com>
  6. ApoTome, Carl Zeiss MicroImaging GmbH, http://www.zeiss.com>
  7. P. Herman, B. P. Maliwal, H.-J. Lin, and J. R. Lakowicz, "Frequency-domain fluorescence microscopy with the LED as a light source," J. Microsc. 203, 176-181 (2001).
    [CrossRef]
  8. C. Moser, T. Mayr, and I. Klimant, "Filter cubes with built-in ultrabright light-emitting diodes as exchangeable excitation light sources in fluorescence microscopy," J. Microsc. 222, 135-140 (2006).
    [CrossRef]
  9. O. Bormuth, J. Howard, and E. Schaffer, "LED illumination for video-enhanced DIC imaging of single microtubules," J. Microsc. 226, 1-5 (2007).
  10. S. X. Jin, J. Li, J. Y. Lin, and H. X. Jiang, "InGaN/GaN quantum well interconnected microdisk light emitting diodes," Appl. Phys. Lett. 77, 3236-3238 (2000).
    [CrossRef]
  11. H. W. Choi, C. W. Jeon, M. D. Dawson, P. R. Edwards, and R. W. Martin, "Fabrication and performance of parallel-addressed InGaN micro-LED arrays," IEEE Photon. Technol. Lett. 15, 510-512 (2003).
    [CrossRef]
  12. C. W. Jeon, H. W. Choi, E. Gu, and M. D. Dawson, "High-density matrix-addressable AlInGaN-based 368-nm microarray light-emitting diodes," IEEE Photon. Technol. Lett. 16, 2421-2423 (2004).
    [CrossRef]
  13. H. X. Zhang, E. Gu, C. W Jeon, Z. Gong, M. D. Dawson, M. A. A. Neil, and P. W. M. French, "Microstripe-array InGaN light-emitting diodes with individually addressable elements," IEEE Photon. Technol. Lett. 18, 1681-1683, (2006).
    [CrossRef]
  14. M. Born, E. Wolf, Principles of Optics, (Pergamon Press, Oxford, 1975).
  15. M. A. A. Neil, R. Juskaitis, and T. Wilson, "Real time 3D fluorescence microscopy by two beam interference illumination," Opt. Commun. 153, 1-4 (1998).
    [CrossRef]
  16. L. H. Schaeffer, D. Schuster, and J. Schaffer, "Structured illumination microscopy: artefact analysis and reduction utilizing a parameter optimization approach," J. Microsc. 216, 165-174 (2004).
    [CrossRef]
  17. P. A. Benedetti, V. Evangelista, D. Guidarini, and S. Vestri, "Electronic multiconfocal points microscopy," Three dimensional microscopy: Image acquisition and processing II, Proc. SPIE 2412, 56-62 (1995).
  18. P. A. Stokseth, "Properties of a defocused optical system," J. Opt. Soc. Am. 59, 1314-1321 (1969).

2007 (1)

O. Bormuth, J. Howard, and E. Schaffer, "LED illumination for video-enhanced DIC imaging of single microtubules," J. Microsc. 226, 1-5 (2007).

2006 (2)

C. Moser, T. Mayr, and I. Klimant, "Filter cubes with built-in ultrabright light-emitting diodes as exchangeable excitation light sources in fluorescence microscopy," J. Microsc. 222, 135-140 (2006).
[CrossRef]

H. X. Zhang, E. Gu, C. W Jeon, Z. Gong, M. D. Dawson, M. A. A. Neil, and P. W. M. French, "Microstripe-array InGaN light-emitting diodes with individually addressable elements," IEEE Photon. Technol. Lett. 18, 1681-1683, (2006).
[CrossRef]

2004 (2)

C. W. Jeon, H. W. Choi, E. Gu, and M. D. Dawson, "High-density matrix-addressable AlInGaN-based 368-nm microarray light-emitting diodes," IEEE Photon. Technol. Lett. 16, 2421-2423 (2004).
[CrossRef]

L. H. Schaeffer, D. Schuster, and J. Schaffer, "Structured illumination microscopy: artefact analysis and reduction utilizing a parameter optimization approach," J. Microsc. 216, 165-174 (2004).
[CrossRef]

2003 (1)

H. W. Choi, C. W. Jeon, M. D. Dawson, P. R. Edwards, and R. W. Martin, "Fabrication and performance of parallel-addressed InGaN micro-LED arrays," IEEE Photon. Technol. Lett. 15, 510-512 (2003).
[CrossRef]

2001 (1)

P. Herman, B. P. Maliwal, H.-J. Lin, and J. R. Lakowicz, "Frequency-domain fluorescence microscopy with the LED as a light source," J. Microsc. 203, 176-181 (2001).
[CrossRef]

2000 (1)

S. X. Jin, J. Li, J. Y. Lin, and H. X. Jiang, "InGaN/GaN quantum well interconnected microdisk light emitting diodes," Appl. Phys. Lett. 77, 3236-3238 (2000).
[CrossRef]

1998 (1)

M. A. A. Neil, R. Juskaitis, and T. Wilson, "Real time 3D fluorescence microscopy by two beam interference illumination," Opt. Commun. 153, 1-4 (1998).
[CrossRef]

1997 (1)

1995 (1)

P. A. Benedetti, V. Evangelista, D. Guidarini, and S. Vestri, "Electronic multiconfocal points microscopy," Three dimensional microscopy: Image acquisition and processing II, Proc. SPIE 2412, 56-62 (1995).

1988 (1)

C. J. R. Sheppard and X. Q. Mao, "Confocal microscopes with slit apertures," J. Mod. Opt. 25, 1169-1185 (1988).

1969 (1)

Appl. Phys. Lett. (1)

S. X. Jin, J. Li, J. Y. Lin, and H. X. Jiang, "InGaN/GaN quantum well interconnected microdisk light emitting diodes," Appl. Phys. Lett. 77, 3236-3238 (2000).
[CrossRef]

IEEE Photon. Technol. Lett. (2)

H. W. Choi, C. W. Jeon, M. D. Dawson, P. R. Edwards, and R. W. Martin, "Fabrication and performance of parallel-addressed InGaN micro-LED arrays," IEEE Photon. Technol. Lett. 15, 510-512 (2003).
[CrossRef]

H. X. Zhang, E. Gu, C. W Jeon, Z. Gong, M. D. Dawson, M. A. A. Neil, and P. W. M. French, "Microstripe-array InGaN light-emitting diodes with individually addressable elements," IEEE Photon. Technol. Lett. 18, 1681-1683, (2006).
[CrossRef]

J. Microsc. (4)

P. Herman, B. P. Maliwal, H.-J. Lin, and J. R. Lakowicz, "Frequency-domain fluorescence microscopy with the LED as a light source," J. Microsc. 203, 176-181 (2001).
[CrossRef]

C. Moser, T. Mayr, and I. Klimant, "Filter cubes with built-in ultrabright light-emitting diodes as exchangeable excitation light sources in fluorescence microscopy," J. Microsc. 222, 135-140 (2006).
[CrossRef]

O. Bormuth, J. Howard, and E. Schaffer, "LED illumination for video-enhanced DIC imaging of single microtubules," J. Microsc. 226, 1-5 (2007).

L. H. Schaeffer, D. Schuster, and J. Schaffer, "Structured illumination microscopy: artefact analysis and reduction utilizing a parameter optimization approach," J. Microsc. 216, 165-174 (2004).
[CrossRef]

J. Mod. Opt. (1)

C. J. R. Sheppard and X. Q. Mao, "Confocal microscopes with slit apertures," J. Mod. Opt. 25, 1169-1185 (1988).

J. Opt. Soc. Am. (1)

Opt. Commun. (1)

M. A. A. Neil, R. Juskaitis, and T. Wilson, "Real time 3D fluorescence microscopy by two beam interference illumination," Opt. Commun. 153, 1-4 (1998).
[CrossRef]

Opt. Lett. (1)

Photon. Technol. Lett. (1)

C. W. Jeon, H. W. Choi, E. Gu, and M. D. Dawson, "High-density matrix-addressable AlInGaN-based 368-nm microarray light-emitting diodes," IEEE Photon. Technol. Lett. 16, 2421-2423 (2004).
[CrossRef]

Proc. SPIE (1)

P. A. Benedetti, V. Evangelista, D. Guidarini, and S. Vestri, "Electronic multiconfocal points microscopy," Three dimensional microscopy: Image acquisition and processing II, Proc. SPIE 2412, 56-62 (1995).

Other (5)

T. Wilson and Barry R. Masters, Confocal Microscopy, (Academic Press, San Diego, 1990).

W. B. Amos and J. G. White, "Direct view confocal imaging systems using a slit aperture," in Handbook of Biological Confocal Microscopy, J. B. Pawley (Plenum, New York, 1995), pp. 403-415.

M. Born, E. Wolf, Principles of Optics, (Pergamon Press, Oxford, 1975).

OptiGrid, QiOptiq Imaging Solutions, http://www.qioptiqimaging.com>

ApoTome, Carl Zeiss MicroImaging GmbH, http://www.zeiss.com>

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