Abstract

Elastin is an essential and widespread structural protein in charge of the integrity on tissues and organs. In this study, we demonstrate that elastin is a major origin of the third-harmonic-generation (THG) contrast under Cr:forsterite laser excitation operating at 1230nm, with selective visualization inside many tissues such as lung tissues and arteries. In vivo imaging of the nude mouse elastic cartilage beneath the hypodermis by epi- THG microscopy keeps the high resolution and contrast in all three dimensions. Combined with second-harmonic-generation microscopy, THG microscopy exhibits the ability to show the extraordinary proliferation of elastic fibers for the ophthalmic disease of pterygium and the capability of distinguishable visualization from collagen.

© 2007 Optical Society of America

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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
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    [CrossRef]

2006 (6)

2005 (3)

2004 (4)

C.-K. Sun, S.-W. Chu, S.-Y. Chen, T.-H. Tsai, T.-M. Liu, C.-Y. Lin, and H.-J. Tsai, "Higher harmonic generation microscopy for developmental biology," J. Struct. Biol. 147, 19-30 (2004).
[CrossRef]

M. Zandvoort, W. Engels, K. Douma, L. Beckers, M. Egbrink, M. Daemen, and D. W. Slaaf, "Two-photon microscopy for imaging of the (atherosclerotic) vascular wall: a proof of concept study," J. Vasc. Res. 41, 54-63 (2004).
[CrossRef]

A. Zoumi, X. Lu, G. S. Kassab, and B. J. Tromberg, "Imaging coronary artery microstructure using second-harmonic and two-photon fluorescence microscopy," Biophys J. 87, 2778-2786 (2004).
[CrossRef]

S.-W. Chu, S.-Y Chen, G.-W. Chern, T.-H. Tsai, Y.-C. Chen, B.-L. Lin, C.-K. Sun, "Studies of χ(2) /χ(3) tensors in submicron-scaled bio-tissues by polarization harmonics optical microscopy," Biophys. J. 86, 3914-3922 (2004).
[CrossRef]

2003 (1)

2002 (1)

S.-W. Chu, I.-S. Chen, T.-M. Liu, C.-K. Sun, S.-P. Lee, B.-L. Lin, P.-C. Cheng, M.-X. Kuo, D.-J. Lin, and H.-L. Liu, "Nonlinear bio-photonic crystal effects revealed with multi-modal nonlinear microscopy," J. Microsc. 208, 190-200 (2002).
[CrossRef]

2001 (5)

T. Osakabe, M. Hayashi, K. Hasegawa, T. Okuaki, T. M. Ritty, R. P. Mecham, H. Wachi, and Y. Seyama, "Age- and gender-related changes in ligament components," Ann. Clin. Biochem. 38, 527-532 (2001).
[CrossRef]

T.-M. Liu, S.-W. Chiu, C.-K Sun, B.-L. Lin, P. C. Cheng, and I. Johnson, "Multi-photon scanning microscopy using a femtosecond Cr:forsterite laser," Scanning 23, 249-254 (2001).

G. A. Blab, P. H. M. Lommerse, L. Cognet, G. S. Harms, and T. Schmidt, "Two-photon excitation action cross-sections of the autofluorescent proteins," Chem. Phys. Lett. 350, 71-77 (2001)
[CrossRef]

P. J. Campagnola, H. A. Clark, W. A. Mohler, A. Lewis, and L. M. Loew, "Second-harmonic imaging microscopy of living cells," J. Biomed. Optics. 6, 277-286(2001).

L. Canioni, S. Rivet, L. Sarger, R. Barille, P. Vacher, and P. Voisin, "Imaging of Ca2+ intracellular dynamics with a third-harmonic generation microscope," Opt. Lett. 26, 515-517 (2001).
[CrossRef]

2000 (5)

I.-J. Wang, F.-R. Hu, P. J. Chen, and C. T. Lin, "Mechanism of abnormal elastin gene express in the piguecular part of pterygia,"Am. J. Pathol. 157, 1269-1276 (2000).

Z. Urban and C. D. Boyd, "Elastic-fiber pathologies: Primary defects in assembly and secondary disorders in transport and delivery," Am. J. Hum. Genet. 67, 4-7 (2000).
[CrossRef]

M. Fukushima, Y. Fukuda, M. Kawamoto, and N. Yamanaka, "Elastosis in lung carcinoma: Immunohistochemical, ultrasturcture and clinical studies," Pathol. Int. 50, 626-635 (2000).
[CrossRef]

E. Georgiou, T. Theodossiou, V. Hovhannisyan, K. Politopoulos, G. S. Rapti, and D. Yova, "Second and third optical harmonic generation in type I collagen, by nanosecond laser irradiation, over a broad spectral region," Opt. Commun. 176, 253-260(2000).
[CrossRef]

T. Parasassi, W. Yu, D. Durbin, L. Kuriashkina, E. Gratton, N. Maeda, and F. Ursini, "Two-photon microscopy of aorta fibers shows proteolysis induced by LDL hydroperoxides," Free Radic Biol. Med. 28, 1589-1597 (2000).
[CrossRef]

1999 (2)

P. J. Campagnola, M. D. Wei, A. Lewis, and L. M. Loew, "Highresolution nonlinear optical imaging of live cells by second harmonic generation," Biophys. J. 77, 3341-3349 (1999).

D. Yelin and Y. Silberberg, "Laser scanning third-harmonic-generation microscopy in biology," Opt. Express 5, 169-175 (1999).

1998 (1)

1997 (1)

1996 (1)

H. F. Carvalho and S. R. Taboga, "Fluorescence and confocal laser scanning microscopy imaging of elastic fibers in hematoxylin-eosin stained sections," Histochem. Cell Biol. 106, 587-592 (1996).

1994 (1)

J. N. Bradbeer, M. Miminucci, and P. Bianco, "Giemsa as a fluorescent stain for mineralized bone," J. Histochem. Cytochem. 42, 677-680 (1994).

1985 (1)

C. Isaacson, H. Greeff, J. F. Murray, J. Posen, and A. Schmaman, "Elastosis in malignant tumours," S. Afr. Med. J. 68, 30-32 (1985).

1981 (1)

S. Roth and I. Freund, "Optical second-harmonic scattering in rat-tail tendon," Biopolymers. 20, 1271-1290 (1981).
[CrossRef]

1980 (1)

R. David, A. Buchner, "Elastosis in benign and malignant salivary gland tumors," Cancer 45, 2301-2310 (1980).
[CrossRef]

1974 (1)

J. G. Azzopardi, R. N. Laurini, "Elastosis in breast cancer," Cancer 33, 174-183 (1974).
[CrossRef]

1972 (1)

J. G. Azzopardi, I. Zayid, "Elastic tissue in tumours of salivary glands," J. Pathol. 107, 149-156 (1972).
[CrossRef]

Adv. Protein Chem. (1)

S. M. Mithieux and A. S. Weiss, "Elastin," Adv. Protein Chem. 70, 437-461(2005).
[CrossRef]

Am. J. Hum. Genet. (1)

Z. Urban and C. D. Boyd, "Elastic-fiber pathologies: Primary defects in assembly and secondary disorders in transport and delivery," Am. J. Hum. Genet. 67, 4-7 (2000).
[CrossRef]

Am. J. Pathol. (1)

I.-J. Wang, F.-R. Hu, P. J. Chen, and C. T. Lin, "Mechanism of abnormal elastin gene express in the piguecular part of pterygia,"Am. J. Pathol. 157, 1269-1276 (2000).

Ann. Clin. Biochem. (1)

T. Osakabe, M. Hayashi, K. Hasegawa, T. Okuaki, T. M. Ritty, R. P. Mecham, H. Wachi, and Y. Seyama, "Age- and gender-related changes in ligament components," Ann. Clin. Biochem. 38, 527-532 (2001).
[CrossRef]

Biophys J. (1)

A. Zoumi, X. Lu, G. S. Kassab, and B. J. Tromberg, "Imaging coronary artery microstructure using second-harmonic and two-photon fluorescence microscopy," Biophys J. 87, 2778-2786 (2004).
[CrossRef]

Biophys. J. (2)

P. J. Campagnola, M. D. Wei, A. Lewis, and L. M. Loew, "Highresolution nonlinear optical imaging of live cells by second harmonic generation," Biophys. J. 77, 3341-3349 (1999).

S.-W. Chu, S.-Y Chen, G.-W. Chern, T.-H. Tsai, Y.-C. Chen, B.-L. Lin, C.-K. Sun, "Studies of χ(2) /χ(3) tensors in submicron-scaled bio-tissues by polarization harmonics optical microscopy," Biophys. J. 86, 3914-3922 (2004).
[CrossRef]

Biopolymers. (1)

S. Roth and I. Freund, "Optical second-harmonic scattering in rat-tail tendon," Biopolymers. 20, 1271-1290 (1981).
[CrossRef]

Cancer (2)

J. G. Azzopardi, R. N. Laurini, "Elastosis in breast cancer," Cancer 33, 174-183 (1974).
[CrossRef]

R. David, A. Buchner, "Elastosis in benign and malignant salivary gland tumors," Cancer 45, 2301-2310 (1980).
[CrossRef]

Chem. Phys. Lett. (1)

G. A. Blab, P. H. M. Lommerse, L. Cognet, G. S. Harms, and T. Schmidt, "Two-photon excitation action cross-sections of the autofluorescent proteins," Chem. Phys. Lett. 350, 71-77 (2001)
[CrossRef]

Cytometry A (1)

T. Boulesteix, A. M. Pena, N. Pages, G. Godeau, M. P. Sauviat, E. Beaurepaire, and M. C. Schanne-Klein, "Micrometer scale ex vivo multiphoton imaging of unstained arterial wall structure," Cytometry A 69, 20-26 (2006)

Free Radic Biol. Med. (1)

T. Parasassi, W. Yu, D. Durbin, L. Kuriashkina, E. Gratton, N. Maeda, and F. Ursini, "Two-photon microscopy of aorta fibers shows proteolysis induced by LDL hydroperoxides," Free Radic Biol. Med. 28, 1589-1597 (2000).
[CrossRef]

Histochem. Cell Biol. (1)

H. F. Carvalho and S. R. Taboga, "Fluorescence and confocal laser scanning microscopy imaging of elastic fibers in hematoxylin-eosin stained sections," Histochem. Cell Biol. 106, 587-592 (1996).

J. Biomed. Optics. (1)

P. J. Campagnola, H. A. Clark, W. A. Mohler, A. Lewis, and L. M. Loew, "Second-harmonic imaging microscopy of living cells," J. Biomed. Optics. 6, 277-286(2001).

J. Histochem. Cytochem. (1)

J. N. Bradbeer, M. Miminucci, and P. Bianco, "Giemsa as a fluorescent stain for mineralized bone," J. Histochem. Cytochem. 42, 677-680 (1994).

J. Microsc. (1)

S.-W. Chu, I.-S. Chen, T.-M. Liu, C.-K. Sun, S.-P. Lee, B.-L. Lin, P.-C. Cheng, M.-X. Kuo, D.-J. Lin, and H.-L. Liu, "Nonlinear bio-photonic crystal effects revealed with multi-modal nonlinear microscopy," J. Microsc. 208, 190-200 (2002).
[CrossRef]

J. Opt. Soc. Am. B (1)

J. Pathol. (1)

J. G. Azzopardi, I. Zayid, "Elastic tissue in tumours of salivary glands," J. Pathol. 107, 149-156 (1972).
[CrossRef]

J. Struct. Biol. (1)

C.-K. Sun, S.-W. Chu, S.-Y. Chen, T.-H. Tsai, T.-M. Liu, C.-Y. Lin, and H.-J. Tsai, "Higher harmonic generation microscopy for developmental biology," J. Struct. Biol. 147, 19-30 (2004).
[CrossRef]

J. Vasc. Res. (1)

M. Zandvoort, W. Engels, K. Douma, L. Beckers, M. Egbrink, M. Daemen, and D. W. Slaaf, "Two-photon microscopy for imaging of the (atherosclerotic) vascular wall: a proof of concept study," J. Vasc. Res. 41, 54-63 (2004).
[CrossRef]

Nat. Methods (1)

D. Debarre, W. Supatto, A.-M. Pena, A. Fabre, T. Tordjmann, L. Combettes, M.-C. Schanne-Klein, and E. Beaurepaire, "Imaging lipid bodies in cells and tissues using third-harmonic generation microscopy," Nat. Methods 3, 47-53 (2006).
[CrossRef]

Opt. Commun. (1)

E. Georgiou, T. Theodossiou, V. Hovhannisyan, K. Politopoulos, G. S. Rapti, and D. Yova, "Second and third optical harmonic generation in type I collagen, by nanosecond laser irradiation, over a broad spectral region," Opt. Commun. 176, 253-260(2000).
[CrossRef]

Opt. Express (5)

Opt. Lett. (5)

Pathol. Int. (1)

M. Fukushima, Y. Fukuda, M. Kawamoto, and N. Yamanaka, "Elastosis in lung carcinoma: Immunohistochemical, ultrasturcture and clinical studies," Pathol. Int. 50, 626-635 (2000).
[CrossRef]

S. Afr. Med. J. (1)

C. Isaacson, H. Greeff, J. F. Murray, J. Posen, and A. Schmaman, "Elastosis in malignant tumours," S. Afr. Med. J. 68, 30-32 (1985).

Scanning (1)

T.-M. Liu, S.-W. Chiu, C.-K Sun, B.-L. Lin, P. C. Cheng, and I. Johnson, "Multi-photon scanning microscopy using a femtosecond Cr:forsterite laser," Scanning 23, 249-254 (2001).

Other (2)

S.-Y. Chen, S.-P. Tai, T.-H. Tsai and C.-K. Sun, "Direct backward-emitted third-harmonic generation and its application to clinical microscopy," in Technical Digest of Conference on Laser and Electro-optics/Quantum Electronics and Laser Science Conference (CLEO/QELS 2005), Baltimore, MD, USA, paper QMI3 (2005).

M. H. Ross, G. I. Kaye, and W. Pawlina, Histology (Lippincott Williams & Wilkins, 2003), Chap 23.

Supplementary Material (2)

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