Abstract

In a previous paper [J. S. Dam et al, Opt. Express 15, 1923 (2007)] we demonstrated computerized “drag-and-drop” optical alignment of a counter-propagating multi-beam based micromanipulation system. By inclusion of image analysis, we report here on the extension of this work to accommodate a completely automated beam-alignment process. Additionally, to maintain a cost-effective and technically less demanding system architecture, we also report on a computer-guided manual alignment procedure. In the manual version, the computer analyzes the initial misalignment and the required compensations for each mirror in the system are calculated. Subsequently, the user is guided in adjusting the mirrors exactly by the requisite amount. This way, all mirrors only need to be moved once. The image analysis utilized in both calibration schemes employs a fitting algorithm to determine the position of beam-center with sub-pixel accuracy, thereby providing “better than human” alignment.

© 2007 Optical Society of America

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References

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  1. P. Kraikivski, B. Pouligny, and R. Dimova, "Implementing both short- and long-working-distance optical trappings into a commercial microscope," Rev. Sci. Instrum. 77, 113703 (2006).
    [CrossRef]
  2. S. A. Tatarkova, A. E. Carruthers, and K. Dholakia, "One-Dimensional Optically Bound Arrays of Microscopic Particles," Phys. Rev. Lett 89, 283901 (2002).
    [CrossRef]
  3. Z. Wang, J. Yin, and Z. Wang, "Guiding and cooling of atoms in an interference field composed of two hollow beams," Opt. Express 14, 9551-9557 (2006).
    [CrossRef] [PubMed]
  4. A. Isomura, N. Magome, M. I. Kohira, K. Yoshikawa, "Toward the stable optical trapping of a droplet with counter laser beams under microgravity," Chem. Phys. Lett. 429, 321-325 (2006).
    [CrossRef]
  5. P. J. Rodrigo, V. R. Daria, and J. Glückstad, "Four-dimensional optical manipulation of colloidal particles," Appl. Phys. Lett. 86, 074103 (2005).
    [CrossRef]
  6. J. Huisken, J. Swoger, and E. H. Stelzer, "Three-dimensional optical manipulation using four collimated intersecting laser beams," Opt. Express 15, 4921-4928 (2007).
    [CrossRef] [PubMed]
  7. J. S. Dam, P. J. Rodrigo, I. R. Perch-Nielsen, C. A. Alonzo, and J. Glückstad, "Computerized "drag-and-drop" alignment of GPC-based optical micromanipulation system," Opt. Express 15, 1923-1931 (2007).
    [CrossRef] [PubMed]
  8. I. R. Perch-Nielsen, P. J. Rodrigo, C. A. Alonzo, and J. Glückstad, "Autonomous and 3D real-time multi-beam manipulation in a microfluidic environment," Opt. Express 14, 12199-12205 (2006).
    [CrossRef] [PubMed]
  9. E. Hecht, Optics (Addison-Wesley, 2002).

2007 (2)

2006 (4)

Z. Wang, J. Yin, and Z. Wang, "Guiding and cooling of atoms in an interference field composed of two hollow beams," Opt. Express 14, 9551-9557 (2006).
[CrossRef] [PubMed]

I. R. Perch-Nielsen, P. J. Rodrigo, C. A. Alonzo, and J. Glückstad, "Autonomous and 3D real-time multi-beam manipulation in a microfluidic environment," Opt. Express 14, 12199-12205 (2006).
[CrossRef] [PubMed]

P. Kraikivski, B. Pouligny, and R. Dimova, "Implementing both short- and long-working-distance optical trappings into a commercial microscope," Rev. Sci. Instrum. 77, 113703 (2006).
[CrossRef]

A. Isomura, N. Magome, M. I. Kohira, K. Yoshikawa, "Toward the stable optical trapping of a droplet with counter laser beams under microgravity," Chem. Phys. Lett. 429, 321-325 (2006).
[CrossRef]

2005 (1)

P. J. Rodrigo, V. R. Daria, and J. Glückstad, "Four-dimensional optical manipulation of colloidal particles," Appl. Phys. Lett. 86, 074103 (2005).
[CrossRef]

2002 (1)

S. A. Tatarkova, A. E. Carruthers, and K. Dholakia, "One-Dimensional Optically Bound Arrays of Microscopic Particles," Phys. Rev. Lett 89, 283901 (2002).
[CrossRef]

Appl. Phys. Lett. (1)

P. J. Rodrigo, V. R. Daria, and J. Glückstad, "Four-dimensional optical manipulation of colloidal particles," Appl. Phys. Lett. 86, 074103 (2005).
[CrossRef]

Chem. Phys. Lett. (1)

A. Isomura, N. Magome, M. I. Kohira, K. Yoshikawa, "Toward the stable optical trapping of a droplet with counter laser beams under microgravity," Chem. Phys. Lett. 429, 321-325 (2006).
[CrossRef]

Opt. Express (4)

Phys. Rev. Lett (1)

S. A. Tatarkova, A. E. Carruthers, and K. Dholakia, "One-Dimensional Optically Bound Arrays of Microscopic Particles," Phys. Rev. Lett 89, 283901 (2002).
[CrossRef]

Rev. Sci. Instrum. (1)

P. Kraikivski, B. Pouligny, and R. Dimova, "Implementing both short- and long-working-distance optical trappings into a commercial microscope," Rev. Sci. Instrum. 77, 113703 (2006).
[CrossRef]

Other (1)

E. Hecht, Optics (Addison-Wesley, 2002).

Supplementary Material (1)

» Media 1: AVI (2490 KB)     

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Figures (6)

Fig. 1.
Fig. 1.

Displaying a binary pattern consisting of randomly distributed curved lines using a spatial light modulator (SLM) produces the same pattern in the focus plane at the sample as described in Ref. 7 and references therein. When the sample surface is aligned with this focused image, a focused image will be visualized on the CCD camera (a). If the sample surface is displaced from the focal plane, the image is blurred (b).

Fig. 2.
Fig. 2.

Focus quality or standard deviation of pixel-intensities as function of motorized stage position. Data shown are from the quick (1) and slow (2) sweep, respectively. The position with largest pixel standard deviation in the slow sweep is the measured focus position.

Fig. 3.
Fig. 3.

To the left are frames showing focused and defocused crosshairs. Their locations are determined with sub-pixel accuracy by quadratic fitting of summed line intensities as shown to the right.

Fig. 4.
Fig. 4.

3D-drawing showing that adjusting a mirror does not only result in an angle being induced (here Δθ), but also some beam translation (here Δs) will occur, which may or may not be on the same axis.

Fig. 5.
Fig. 5.

Similar to the previously described experimental setup [7], both the upper and lower beam paths have two adjustable mirror mounts. Each mirror can be adjusted around two axes.

Fig. 6.
Fig. 6.

(AVI: 2.4 MB) Figure showing initial focused and defocused crosshairs positions (a+b). A target crosshair is displayed in the defocused image (c). The user adjusts the first mirror until crosshairs overlap (d). Move to focus, and display centered crosshair for user to displace second mirror (e+f). Finally the image is defocused to proof correct calibration (g). [Media 1]

Equations (3)

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Δ x A Δ y A Δ u A Δ v A = x A displaced y A displaced u A displaced v A displaced x initial y initial u initial v initial
a Δ x A Δ y A Δ u A Δ v A + b Δ x B Δ y B Δ u B Δ v B + c Δ x C Δ y C Δ u C Δ v C + d Δ x D Δ y D Δ u D Δ v D = Δ x Δ y Δ u Δ v
Δ u ˜ Δ v ˜ = a Δ u A Δ v A + b Δ u B Δ v B = Δ u Δ v c Δ u c Δ v c d Δ u c Δ v c

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