Abstract

A ferroelectric liquid crystal spatial light modulator is used to generate up to 24 independently controllable traps in a holographic optical tweezers system using time-multiplexed Fresnel zone plates. For use in biological applications, helical zone plates are used to generate Laguerre-Gaussian laser modes. The high speed switching of the ferroelectric device together with recent advances in computer technology enable fast, smooth movement of traps that can be independently controlled in real time. This is demonstrated by the trapping and manipulation of yeast cells and fungal spores.

© 2006 Optical Society of America

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    [CrossRef] [PubMed]
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    [CrossRef] [PubMed]
  4. J E Molloy, "Optical chopsticks: digital synthesis of multiple optical traps," Methods in cell biology 55205-215 (1998)
    [CrossRef]
  5. J Liesener, M Reicherter, T Haist, H J Tiziani "Multi-functional optical tweezers using computer gererated holograms," Opt. Commun. 185, 77 (2000)
    [CrossRef]
  6. W J Hossack, E Theofanidou, J Crain, "High speed holographic optical tweezers using a ferroelectric liquid crystal microdisplay," Opt. Express 11, 2053-2058 (2004).
    [CrossRef]
  7. M J Padgett, J Courtial, Z J Laczik, G Sinclair, J Leach, P Jordan, G Gibson, E Yao, "Interactive application in holographic optical tweezers of a multiplane Gerchberg-Saxton algorithm for three-dimensional light shaping," Opt. Express 12, 1665-1670 (2004).
    [CrossRef] [PubMed]
  8. R L Eriksen, V R Daria, P J Rodrigo, J Gluckstadt, "Computer controlled orientation of multiple optically trapped particles," Microelectronic Engineering 67-68, 872 (2003)
    [CrossRef]
  9. A Jesacher, S Furhapter, S Bernet, M Ritsch-Marte, "Diffractive optical tweezers in the Fresnel regime," Opt. Express 12,2243 (2004).
    [CrossRef] [PubMed]
  10. Sun developer network, http//java.sun.com
  11. OpenGL , http://www.opengl.org/
  12. D G Vass, W J Hossack, S Nath, A O’Hara, I D Rankin, M W G Snook, I Underwood, M R Worboys, M S Griffith, S Radcliffe, D Macintosh, J Harkness, B Mitchel, G Rickard, J Harris, E Judd, "A high resolution, full colour head mounted ferroelectric liquid crystal over silicon display," Ferroelectrics 213, 209-218 (1998)
    [CrossRef]
  13. CRL Opto Ltd, http://www.crlopto.com (Name change in progress to; Forth Dimension Displays, http://www.forthdd.com)
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    [CrossRef]
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  16. T Haist, M Reicherter, M Wu, L Selfert, "Using graphics boards to compute holograms," Computing in science and engineering 8, 8-13 (2006)
    [CrossRef]
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    [CrossRef] [PubMed]
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    [CrossRef]

2006 (1)

T Haist, M Reicherter, M Wu, L Selfert, "Using graphics boards to compute holograms," Computing in science and engineering 8, 8-13 (2006)
[CrossRef]

2005 (1)

N Ameborg, H Siegumfeldt, G H Andersen, P Nissen, V R Daria, P J Rodrigo, J Gluckstad, "Interactive optical trapping shows that confinement is a determinant of growth in a mixed yeast culture," FEMS Microbiology Lett. 245155 (2005)
[CrossRef]

2004 (4)

2003 (1)

R L Eriksen, V R Daria, P J Rodrigo, J Gluckstadt, "Computer controlled orientation of multiple optically trapped particles," Microelectronic Engineering 67-68, 872 (2003)
[CrossRef]

2000 (1)

J Liesener, M Reicherter, T Haist, H J Tiziani "Multi-functional optical tweezers using computer gererated holograms," Opt. Commun. 185, 77 (2000)
[CrossRef]

1998 (2)

J E Molloy, "Optical chopsticks: digital synthesis of multiple optical traps," Methods in cell biology 55205-215 (1998)
[CrossRef]

D G Vass, W J Hossack, S Nath, A O’Hara, I D Rankin, M W G Snook, I Underwood, M R Worboys, M S Griffith, S Radcliffe, D Macintosh, J Harkness, B Mitchel, G Rickard, J Harris, E Judd, "A high resolution, full colour head mounted ferroelectric liquid crystal over silicon display," Ferroelectrics 213, 209-218 (1998)
[CrossRef]

1997 (1)

1991 (1)

1986 (1)

Ameborg, N

N Ameborg, H Siegumfeldt, G H Andersen, P Nissen, V R Daria, P J Rodrigo, J Gluckstad, "Interactive optical trapping shows that confinement is a determinant of growth in a mixed yeast culture," FEMS Microbiology Lett. 245155 (2005)
[CrossRef]

Andersen, G H

N Ameborg, H Siegumfeldt, G H Andersen, P Nissen, V R Daria, P J Rodrigo, J Gluckstad, "Interactive optical trapping shows that confinement is a determinant of growth in a mixed yeast culture," FEMS Microbiology Lett. 245155 (2005)
[CrossRef]

Ashkin, A

Axner, O

Bernet, S

Bjorkholm, J E

Chu, S

Cooper, J

Courtail, J

Courtial, J

Crain, J

Daria, V R

N Ameborg, H Siegumfeldt, G H Andersen, P Nissen, V R Daria, P J Rodrigo, J Gluckstad, "Interactive optical trapping shows that confinement is a determinant of growth in a mixed yeast culture," FEMS Microbiology Lett. 245155 (2005)
[CrossRef]

R L Eriksen, V R Daria, P J Rodrigo, J Gluckstadt, "Computer controlled orientation of multiple optically trapped particles," Microelectronic Engineering 67-68, 872 (2003)
[CrossRef]

Dziedzic, J M

Eriksen, R L

R L Eriksen, V R Daria, P J Rodrigo, J Gluckstadt, "Computer controlled orientation of multiple optically trapped particles," Microelectronic Engineering 67-68, 872 (2003)
[CrossRef]

fallman, E

Furhapter, S

Gibson, G

Gluckstad, J

N Ameborg, H Siegumfeldt, G H Andersen, P Nissen, V R Daria, P J Rodrigo, J Gluckstad, "Interactive optical trapping shows that confinement is a determinant of growth in a mixed yeast culture," FEMS Microbiology Lett. 245155 (2005)
[CrossRef]

Gluckstadt, J

R L Eriksen, V R Daria, P J Rodrigo, J Gluckstadt, "Computer controlled orientation of multiple optically trapped particles," Microelectronic Engineering 67-68, 872 (2003)
[CrossRef]

Griffith, M S

D G Vass, W J Hossack, S Nath, A O’Hara, I D Rankin, M W G Snook, I Underwood, M R Worboys, M S Griffith, S Radcliffe, D Macintosh, J Harkness, B Mitchel, G Rickard, J Harris, E Judd, "A high resolution, full colour head mounted ferroelectric liquid crystal over silicon display," Ferroelectrics 213, 209-218 (1998)
[CrossRef]

Haist, T

T Haist, M Reicherter, M Wu, L Selfert, "Using graphics boards to compute holograms," Computing in science and engineering 8, 8-13 (2006)
[CrossRef]

J Liesener, M Reicherter, T Haist, H J Tiziani "Multi-functional optical tweezers using computer gererated holograms," Opt. Commun. 185, 77 (2000)
[CrossRef]

Harkness, J

D G Vass, W J Hossack, S Nath, A O’Hara, I D Rankin, M W G Snook, I Underwood, M R Worboys, M S Griffith, S Radcliffe, D Macintosh, J Harkness, B Mitchel, G Rickard, J Harris, E Judd, "A high resolution, full colour head mounted ferroelectric liquid crystal over silicon display," Ferroelectrics 213, 209-218 (1998)
[CrossRef]

Harris, J

D G Vass, W J Hossack, S Nath, A O’Hara, I D Rankin, M W G Snook, I Underwood, M R Worboys, M S Griffith, S Radcliffe, D Macintosh, J Harkness, B Mitchel, G Rickard, J Harris, E Judd, "A high resolution, full colour head mounted ferroelectric liquid crystal over silicon display," Ferroelectrics 213, 209-218 (1998)
[CrossRef]

Heckenberg, N R

Hossack, W J

W J Hossack, E Theofanidou, J Crain, "High speed holographic optical tweezers using a ferroelectric liquid crystal microdisplay," Opt. Express 11, 2053-2058 (2004).
[CrossRef]

D G Vass, W J Hossack, S Nath, A O’Hara, I D Rankin, M W G Snook, I Underwood, M R Worboys, M S Griffith, S Radcliffe, D Macintosh, J Harkness, B Mitchel, G Rickard, J Harris, E Judd, "A high resolution, full colour head mounted ferroelectric liquid crystal over silicon display," Ferroelectrics 213, 209-218 (1998)
[CrossRef]

Jesacher, A

Jordan, P

Judd, E

D G Vass, W J Hossack, S Nath, A O’Hara, I D Rankin, M W G Snook, I Underwood, M R Worboys, M S Griffith, S Radcliffe, D Macintosh, J Harkness, B Mitchel, G Rickard, J Harris, E Judd, "A high resolution, full colour head mounted ferroelectric liquid crystal over silicon display," Ferroelectrics 213, 209-218 (1998)
[CrossRef]

Lackzik, Z J

Laczik, Z J

Leach, J

Liesener, J

J Liesener, M Reicherter, T Haist, H J Tiziani "Multi-functional optical tweezers using computer gererated holograms," Opt. Commun. 185, 77 (2000)
[CrossRef]

Macintosh, D

D G Vass, W J Hossack, S Nath, A O’Hara, I D Rankin, M W G Snook, I Underwood, M R Worboys, M S Griffith, S Radcliffe, D Macintosh, J Harkness, B Mitchel, G Rickard, J Harris, E Judd, "A high resolution, full colour head mounted ferroelectric liquid crystal over silicon display," Ferroelectrics 213, 209-218 (1998)
[CrossRef]

McDuff, R

Mitchel, B

D G Vass, W J Hossack, S Nath, A O’Hara, I D Rankin, M W G Snook, I Underwood, M R Worboys, M S Griffith, S Radcliffe, D Macintosh, J Harkness, B Mitchel, G Rickard, J Harris, E Judd, "A high resolution, full colour head mounted ferroelectric liquid crystal over silicon display," Ferroelectrics 213, 209-218 (1998)
[CrossRef]

Molloy, J E

J E Molloy, "Optical chopsticks: digital synthesis of multiple optical traps," Methods in cell biology 55205-215 (1998)
[CrossRef]

Nath, S

D G Vass, W J Hossack, S Nath, A O’Hara, I D Rankin, M W G Snook, I Underwood, M R Worboys, M S Griffith, S Radcliffe, D Macintosh, J Harkness, B Mitchel, G Rickard, J Harris, E Judd, "A high resolution, full colour head mounted ferroelectric liquid crystal over silicon display," Ferroelectrics 213, 209-218 (1998)
[CrossRef]

Nissen, P

N Ameborg, H Siegumfeldt, G H Andersen, P Nissen, V R Daria, P J Rodrigo, J Gluckstad, "Interactive optical trapping shows that confinement is a determinant of growth in a mixed yeast culture," FEMS Microbiology Lett. 245155 (2005)
[CrossRef]

O’Hara, A

D G Vass, W J Hossack, S Nath, A O’Hara, I D Rankin, M W G Snook, I Underwood, M R Worboys, M S Griffith, S Radcliffe, D Macintosh, J Harkness, B Mitchel, G Rickard, J Harris, E Judd, "A high resolution, full colour head mounted ferroelectric liquid crystal over silicon display," Ferroelectrics 213, 209-218 (1998)
[CrossRef]

Padgett, M J

Radcliffe, S

D G Vass, W J Hossack, S Nath, A O’Hara, I D Rankin, M W G Snook, I Underwood, M R Worboys, M S Griffith, S Radcliffe, D Macintosh, J Harkness, B Mitchel, G Rickard, J Harris, E Judd, "A high resolution, full colour head mounted ferroelectric liquid crystal over silicon display," Ferroelectrics 213, 209-218 (1998)
[CrossRef]

Rankin, I D

D G Vass, W J Hossack, S Nath, A O’Hara, I D Rankin, M W G Snook, I Underwood, M R Worboys, M S Griffith, S Radcliffe, D Macintosh, J Harkness, B Mitchel, G Rickard, J Harris, E Judd, "A high resolution, full colour head mounted ferroelectric liquid crystal over silicon display," Ferroelectrics 213, 209-218 (1998)
[CrossRef]

Reicherter, M

T Haist, M Reicherter, M Wu, L Selfert, "Using graphics boards to compute holograms," Computing in science and engineering 8, 8-13 (2006)
[CrossRef]

J Liesener, M Reicherter, T Haist, H J Tiziani "Multi-functional optical tweezers using computer gererated holograms," Opt. Commun. 185, 77 (2000)
[CrossRef]

Rickard, G

D G Vass, W J Hossack, S Nath, A O’Hara, I D Rankin, M W G Snook, I Underwood, M R Worboys, M S Griffith, S Radcliffe, D Macintosh, J Harkness, B Mitchel, G Rickard, J Harris, E Judd, "A high resolution, full colour head mounted ferroelectric liquid crystal over silicon display," Ferroelectrics 213, 209-218 (1998)
[CrossRef]

Ritsch-Marte, M

Rodrigo, P J

N Ameborg, H Siegumfeldt, G H Andersen, P Nissen, V R Daria, P J Rodrigo, J Gluckstad, "Interactive optical trapping shows that confinement is a determinant of growth in a mixed yeast culture," FEMS Microbiology Lett. 245155 (2005)
[CrossRef]

R L Eriksen, V R Daria, P J Rodrigo, J Gluckstadt, "Computer controlled orientation of multiple optically trapped particles," Microelectronic Engineering 67-68, 872 (2003)
[CrossRef]

Selfert, L

T Haist, M Reicherter, M Wu, L Selfert, "Using graphics boards to compute holograms," Computing in science and engineering 8, 8-13 (2006)
[CrossRef]

Siegumfeldt, H

N Ameborg, H Siegumfeldt, G H Andersen, P Nissen, V R Daria, P J Rodrigo, J Gluckstad, "Interactive optical trapping shows that confinement is a determinant of growth in a mixed yeast culture," FEMS Microbiology Lett. 245155 (2005)
[CrossRef]

Sinclair, G

Smith, C P

Snook, M W G

D G Vass, W J Hossack, S Nath, A O’Hara, I D Rankin, M W G Snook, I Underwood, M R Worboys, M S Griffith, S Radcliffe, D Macintosh, J Harkness, B Mitchel, G Rickard, J Harris, E Judd, "A high resolution, full colour head mounted ferroelectric liquid crystal over silicon display," Ferroelectrics 213, 209-218 (1998)
[CrossRef]

Theofanidou, E

Tiziani, H J

J Liesener, M Reicherter, T Haist, H J Tiziani "Multi-functional optical tweezers using computer gererated holograms," Opt. Commun. 185, 77 (2000)
[CrossRef]

Underwood, I

D G Vass, W J Hossack, S Nath, A O’Hara, I D Rankin, M W G Snook, I Underwood, M R Worboys, M S Griffith, S Radcliffe, D Macintosh, J Harkness, B Mitchel, G Rickard, J Harris, E Judd, "A high resolution, full colour head mounted ferroelectric liquid crystal over silicon display," Ferroelectrics 213, 209-218 (1998)
[CrossRef]

Vass, D G

D G Vass, W J Hossack, S Nath, A O’Hara, I D Rankin, M W G Snook, I Underwood, M R Worboys, M S Griffith, S Radcliffe, D Macintosh, J Harkness, B Mitchel, G Rickard, J Harris, E Judd, "A high resolution, full colour head mounted ferroelectric liquid crystal over silicon display," Ferroelectrics 213, 209-218 (1998)
[CrossRef]

White, A G

Worboys, M R

D G Vass, W J Hossack, S Nath, A O’Hara, I D Rankin, M W G Snook, I Underwood, M R Worboys, M S Griffith, S Radcliffe, D Macintosh, J Harkness, B Mitchel, G Rickard, J Harris, E Judd, "A high resolution, full colour head mounted ferroelectric liquid crystal over silicon display," Ferroelectrics 213, 209-218 (1998)
[CrossRef]

Wu, M

T Haist, M Reicherter, M Wu, L Selfert, "Using graphics boards to compute holograms," Computing in science and engineering 8, 8-13 (2006)
[CrossRef]

Yao, E

Appl. Opt. (1)

Computing in science and engineering (1)

T Haist, M Reicherter, M Wu, L Selfert, "Using graphics boards to compute holograms," Computing in science and engineering 8, 8-13 (2006)
[CrossRef]

FEMS Microbiology Lett. (1)

N Ameborg, H Siegumfeldt, G H Andersen, P Nissen, V R Daria, P J Rodrigo, J Gluckstad, "Interactive optical trapping shows that confinement is a determinant of growth in a mixed yeast culture," FEMS Microbiology Lett. 245155 (2005)
[CrossRef]

Ferroelectrics (1)

D G Vass, W J Hossack, S Nath, A O’Hara, I D Rankin, M W G Snook, I Underwood, M R Worboys, M S Griffith, S Radcliffe, D Macintosh, J Harkness, B Mitchel, G Rickard, J Harris, E Judd, "A high resolution, full colour head mounted ferroelectric liquid crystal over silicon display," Ferroelectrics 213, 209-218 (1998)
[CrossRef]

Methods in cell biology (1)

J E Molloy, "Optical chopsticks: digital synthesis of multiple optical traps," Methods in cell biology 55205-215 (1998)
[CrossRef]

Microelectronic Engineering (1)

R L Eriksen, V R Daria, P J Rodrigo, J Gluckstadt, "Computer controlled orientation of multiple optically trapped particles," Microelectronic Engineering 67-68, 872 (2003)
[CrossRef]

Opt. Commun. (1)

J Liesener, M Reicherter, T Haist, H J Tiziani "Multi-functional optical tweezers using computer gererated holograms," Opt. Commun. 185, 77 (2000)
[CrossRef]

Opt. Express (4)

Opt. Lett. (2)

Other (5)

K Dholakia, G Spalding, M MacDonald, "Optical tweezers: The next generation," Physics world15-10 (Oct 2002)

CRL Opto Ltd, http://www.crlopto.com (Name change in progress to; Forth Dimension Displays, http://www.forthdd.com)

N R Heckenberg, M E J Friese, T A Niemenen, H Rubinsztein-Dunlop, "Mechanical effects of optical vortices," M Vasnetsov (ed) Optical Vortices (Horizons in World Physics)228 (Nova Science Publishers, 1999) pp 75-105

Sun developer network, http//java.sun.com

OpenGL , http://www.opengl.org/

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Figures (6)

Fig. 1.
Fig. 1.

(Left) Schematic diagram of optical tweezers showing straight reflection off SLM. (Right) Branch of same diagram showing +1 and -1 (red and blue) orders due to zone plate displayed on SLM. Movable lens moved to bring -1 order into image plane.

Fig. 2.
Fig. 2.

(a): Oscilloscope trace for 1 trap. Shows 1/60th second frame rate. (b): Oscilloscope trace for 24 traps; slightly displaced to invoke device to redraw for each trap. Shows 24 individual peaks per cycle

Fig. 3.
Fig. 3.

Zone plate images generated entirely from polygons. (a)(crude): l = 1 zone plate using 8 polygons per revolution. (b)(fine): same zone plate using 50 polygons per revolution. (c): Two l = 2 LG multiplexed zone plates (fine).

Fig. 4.
Fig. 4.

Trapped fluorescent PMMA particles. (a): circle, 12 traps. (b): smiley face, 24 trap attempt. 3D trapping. (c): 6 particles in a line in the same plane. (d): 6 particles in a line in sloping plane. Image sizes approx 20×20μm

Fig. 5.
Fig. 5.

Four cells of Sacchromyces cervisiae cells trapped with l = 1 LG beams. The group of cells in (A) were moved closer together (B) and then their configuration was changed (C). Bar = 5μm.

Fig. 6.
Fig. 6.

Six Sacchromyces cervisiae cells trapped with l = 4 LG beams. The group of cells in (A) was moved apart (B). Bar = 10μm.

Equations (2)

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= ( n + 1 2 ) π + k r 2 2 R
r = 2 R k ( ( n + 1 2 ) π )

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