Abstract

A supercontinuum light source generated with a femtosecond Ti:Sapphire oscillator has been used to obtain both vibrational and two-photon excitation fluorescence (TPEF) images of a living cell simultaneously at different wavelengths. Owing to an ultrabroadband spectral profile of the supercontinuum, multiple vibrational resonances have been detected through coherent anti-Stokes Raman scattering (CARS) process. In addition to the multiplex CARS process, multiple electronic states can be excited due to the broadband electronic two-photon excitation using the supercontinuum, giving rise to a two-photon excitation fluorescence (TPEF) signal. Using a living yeast cell whose nucleus is labeled by green fluorescent protein (GFP), we have succeeded in visualizing organelles such as mitochondria, septum, and nucleus through the CARS and the TPEF processes. The supercontinuum enables us to perform unique multi-nonlinear optical imaging through two different nonlinear optical processes.

© 2006 Optical Society of America

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  1. G. J. Puppels, F. F. M. De Mul, C. Otto, J. Greve, M. Robert-Nicoud, D. J. Arndt-Jovin, and T. M. Jovin, "Studying single living cells and chromosomes by confocal Raman microspectroscopy," Nature (London, United Kingdom) 347,301-303 (1990).
    [CrossRef] [PubMed]
  2. G. J. Puppels, J. H. Olminkhof, G. M. Segers-Nolten, C. Otto, F. F. de Mul, and J. Greve, "Laser irradiation and Raman spectroscopy of single living cells and chromosomes: sample degradation occurs with 514.5 nm but not with 660 nm laser light," Exp. Cell Res. 195,361-367 (1991).
    [CrossRef] [PubMed]
  3. Y. Takai, T. Masuko, and H. Takeuchi, "Lipid structure of cytotoxic granules in living human killer T lymphocytes studied by Raman microspectroscopy," Biochim. Biophys. Acta 1335,199-208 (1997).
    [CrossRef] [PubMed]
  4. C. Otto, N. M. Sijtsema, and J. Greve, "Confocal Raman microspectroscopy of the activation of single neutrophilic granulocytes," Eur. Biophys. J. 27,582-589 (1998).
    [CrossRef] [PubMed]
  5. Y.-S. Huang, T. Karashima, M. Yamamoto, and H. Hamaguchi, "Molecular-level pursuit of yeast mitosis by time- and space-resolved Raman spectroscopy," J. Raman Spectrosc. 34,1-3 (2003).
    [CrossRef]
  6. Y.-S. Huang, T. Karashima, M. Yamamoto, T. Ogura, and H. Hamaguchi, "Raman spectroscopic signature of life in a living yeast cell," J. Raman Spectrosc. 35,525-526 (2004).
    [CrossRef]
  7. Y.-S. Huang, T. Karashima, M. Yamamoto, and H. Hamaguchi, "Molecular-level investigation of the structure, transformation, and bioactivity of single living fission yeast cells by time- and space-resolved Raman Spectroscopy," Biochemistry 44,10009-10019 (2005).
    [CrossRef] [PubMed]
  8. Y. Naito, A. Toh-e,and H.-o. Hamaguchi, "In vivo time-resolved Raman imaging of a spontaneous death process of a single budding yeast cell," J. Raman Spectrosc. 36,837-839 (2005).
    [CrossRef]
  9. A. Zumbusch, G. R. Holtom, and X. S. Xie, "Three-dimensional vibrational imaging by coherent anti-stokes raman scattering," Phys. Rev. Lett. 82,4142-4145 (1999).
    [CrossRef]
  10. J.-X. Cheng, Y. K. Jia, G. Zheng, and X. S. Xie, "Laser-scanning coherent anti-Stokes Raman scattering microscopy and applications to cell biology," Biophys. J. 83,502-509 (2002).
    [CrossRef] [PubMed]
  11. M. Hashimoto, T. Araki, and S. Kawata, "Molecular vibration imaging in the fingerprint region by use of coherent anti-Stokes Raman scattering microscopy with a collinear configuration," Opt. Lett. 25,1768-1770 (2000).
    [CrossRef]
  12. G. W. H. Wurpel, J. M. Schins, and M. Mueller, "Chemical specificity in three-dimensional imaging with multiplex coherent anti-Stokes Raman scattering microscopy," Opt. Lett. 27,1093-1095 (2002).
    [CrossRef]
  13. H. N. Paulsen, K. M. Hilligsoe, J. Thogersen, S. R. Keiding, and J. J. Larsen, "Coherent anti-Stokes Raman scattering microscopy with a photonic crystal fiber based light source," Opt. Lett. 28,1123-1125 (2003).
    [CrossRef] [PubMed]
  14. R. D. Schaller, J. Ziegelbauer, L. F. Lee, L. H. Haber, and R. J. Saykally, "Chemically selective imaging of subcellular structure in human hepatocytes with coherent anti-stokes Raman scattering (CARS) near-field scanning optical microscopy (NSOM)," J. Phys. Chem. B 106,8489-8492 (2002).
    [CrossRef]
  15. T. Ichimura, N. Hayazawa, M. Hashimoto, Y. Inouye, and S. Kawata, "Tip-enhanced coherent anti-stokes Raman scattering for vibrational nanoimaging," Phys. Rev. Lett. 92,220801 (2004).
    [CrossRef] [PubMed]
  16. C. Otto, A. Voroshilov, S. G. Kruglik, and J. Greve, "Vibrational bands of luminescent zinc(II)-octaethyl-porphyrin using a polarization-sensitive "microscopic" multiplex CARS technique," J. Raman Spectrosc. 32,495-501 (2001).
    [CrossRef]
  17. J.-X. Cheng, A. Volkmer, L. D. Book, and X. S. Xie, "Multiplex coherent anti-stokes raman scattering microspectroscopy and study of lipid vesicles," J. Phys. Chem. B 106,8493-8498 (2002).
    [CrossRef]
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    [CrossRef]
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  20. H. Kano, and H. Hamaguchi, "Dispersion-compensated supercontinuum generation for ultrabroadband multiplex coherent anti-Stokes Raman scattering spectroscopy," J. Raman Spectrosc., in press.
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  25. H. Kano, and H. Hamaguchi, "Ultrabroadband (>2500 cm-1) Multiplex coherent anti-stokes raman scattering microspectroscopy using a supercontinuum generated from a photonic crystal fiber," Appl. Phys. Lett. 86,121113-121115 (2005).
    [CrossRef] [PubMed]
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    [CrossRef] [PubMed]
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    [CrossRef]
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    [CrossRef] [PubMed]
  30. J. A. Palero, V. O. Boer, J. C. Vijverberg, H. C. Gerritsen, and H. J. C. M. Sterenborg, "Short-wavelength two-photon excitation fluorescence microscopy of tryptophan with a photonic crystal fiber based light source," Opt. Express 13,5363-5368 (2005).
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2005

Y.-S. Huang, T. Karashima, M. Yamamoto, and H. Hamaguchi, "Molecular-level investigation of the structure, transformation, and bioactivity of single living fission yeast cells by time- and space-resolved Raman Spectroscopy," Biochemistry 44,10009-10019 (2005).
[CrossRef] [PubMed]

Y. Naito, A. Toh-e,and H.-o. Hamaguchi, "In vivo time-resolved Raman imaging of a spontaneous death process of a single budding yeast cell," J. Raman Spectrosc. 36,837-839 (2005).
[CrossRef]

H. Kano, and H. Hamaguchi, "Ultrabroadband (>2500 cm-1) Multiplex coherent anti-stokes raman scattering microspectroscopy using a supercontinuum generated from a photonic crystal fiber," Appl. Phys. Lett. 86,121113-121115 (2005).
[CrossRef] [PubMed]

H. Kano, and H. Hamaguchi, "Vibrationally resonant imaging of a single living cell by supercontinuum-based multiplex coherent anti-Stokes Raman scattering microspectroscopy," Opt. Express 13,1322-1327 (2005). http://www.opticsexpress.org/abstract.cfm?URI=OPEX-13-4-1322
[CrossRef] [PubMed]

S.-H. Lim, A. G. Caster, and S. R. Leone, "Single-pulse phase-control interferometric coherent anti-Stokes Raman scattering spectroscopy," Phys. Rev. A 30,2805-2807 (2005).

I. G. Petrov, and V. V. Yakovlev, "Enhancing red-shifted white-light continuum generation in optical fibers for applications in nonlinear Raman microscopy," Opt. Express 13,1299 (2005).
[CrossRef] [PubMed]

K. Isobe, W. Watanabe, S. Matsunaga, T. Higashi, K. Fukui, and K. Itoh, "Multi-spectral two-photon excited fluorescence microscopy using supercontinuum light source," Jpn. J. Appl. Phys. Part 2 44,L167-L169 (2005).
[CrossRef] [PubMed]

J. A. Palero, V. O. Boer, J. C. Vijverberg, H. C. Gerritsen, and H. J. C. M. Sterenborg, "Short-wavelength two-photon excitation fluorescence microscopy of tryptophan with a photonic crystal fiber based light source," Opt. Express 13,5363-5368 (2005).

H. Wang, Y. Fu, P. Zickmund, R. Shi, and J.-X. Cheng, "Coherent anti-Stokes Raman scattering imaging of live spinal tissues," Biophys. J. 89,581-591 (2005).

2004

S. O. Konorov, D. A. Akimov, E. E. Serebryannikov, A. A. Ivanov, M. V. Alfimov, and A. M. Zheltikov, "Cross-correlation frequency-resolved optical gating coherent anti-Stokes Raman scattering with frequency-converting photonic-crystal fibers," Phys. Rev. E 70,057601 (2004).
[CrossRef]

T. W. Kee, and M. T. Cicerone, "Simple approach to one-laser, broadband coherent anti-Stokes Raman scattering microscopy," Opt. Lett. 29,2701-2703 (2004).
[CrossRef] [PubMed]

C. McConnell, and E. Riis, "Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with fura-2," Phys. Med. Biol. 49,4757-4763 (2004).
[CrossRef]

Y.-S. Huang, T. Karashima, M. Yamamoto, T. Ogura, and H. Hamaguchi, "Raman spectroscopic signature of life in a living yeast cell," J. Raman Spectrosc. 35,525-526 (2004).
[CrossRef]

T. Ichimura, N. Hayazawa, M. Hashimoto, Y. Inouye, and S. Kawata, "Tip-enhanced coherent anti-stokes Raman scattering for vibrational nanoimaging," Phys. Rev. Lett. 92,220801 (2004).
[CrossRef] [PubMed]

2003

H. N. Paulsen, K. M. Hilligsoe, J. Thogersen, S. R. Keiding, and J. J. Larsen, "Coherent anti-Stokes Raman scattering microscopy with a photonic crystal fiber based light source," Opt. Lett. 28,1123-1125 (2003).
[CrossRef] [PubMed]

Y.-S. Huang, T. Karashima, M. Yamamoto, and H. Hamaguchi, "Molecular-level pursuit of yeast mitosis by time- and space-resolved Raman spectroscopy," J. Raman Spectrosc. 34,1-3 (2003).
[CrossRef]

2002

R. D. Schaller, J. Ziegelbauer, L. F. Lee, L. H. Haber, and R. J. Saykally, "Chemically selective imaging of subcellular structure in human hepatocytes with coherent anti-stokes Raman scattering (CARS) near-field scanning optical microscopy (NSOM)," J. Phys. Chem. B 106,8489-8492 (2002).
[CrossRef]

J.-X. Cheng, Y. K. Jia, G. Zheng, and X. S. Xie, "Laser-scanning coherent anti-Stokes Raman scattering microscopy and applications to cell biology," Biophys. J. 83,502-509 (2002).
[CrossRef] [PubMed]

J.-X. Cheng, A. Volkmer, L. D. Book, and X. S. Xie, "Multiplex coherent anti-stokes raman scattering microspectroscopy and study of lipid vesicles," J. Phys. Chem. B 106,8493-8498 (2002).
[CrossRef]

D. Oron, N. Dudovich, and Y. Silberberg, "Single-pulse phase-contrast nonlinear Raman Spectroscopy," Phys. Rev. Lett. 89,273001 (2002).
[CrossRef]

D. Oron, N. Dudovich, D. Yelin, and Y. Silberberg, "Narrow-band coherent anti-stokes Raman signals from broad-band pulses," Phys. Rev. Lett. 88,063004 (2002).
[CrossRef] [PubMed]

G. W. H. Wurpel, J. M. Schins, and M. Mueller, "Chemical specificity in three-dimensional imaging with multiplex coherent anti-Stokes Raman scattering microscopy," Opt. Lett. 27,1093-1095 (2002).
[CrossRef]

2001

C. Otto, A. Voroshilov, S. G. Kruglik, and J. Greve, "Vibrational bands of luminescent zinc(II)-octaethyl-porphyrin using a polarization-sensitive "microscopic" multiplex CARS technique," J. Raman Spectrosc. 32,495-501 (2001).
[CrossRef]

2000

1999

A. Zumbusch, G. R. Holtom, and X. S. Xie, "Three-dimensional vibrational imaging by coherent anti-stokes raman scattering," Phys. Rev. Lett. 82,4142-4145 (1999).
[CrossRef]

1998

C. Otto, N. M. Sijtsema, and J. Greve, "Confocal Raman microspectroscopy of the activation of single neutrophilic granulocytes," Eur. Biophys. J. 27,582-589 (1998).
[CrossRef] [PubMed]

1997

Y. Takai, T. Masuko, and H. Takeuchi, "Lipid structure of cytotoxic granules in living human killer T lymphocytes studied by Raman microspectroscopy," Biochim. Biophys. Acta 1335,199-208 (1997).
[CrossRef] [PubMed]

1991

G. J. Puppels, J. H. Olminkhof, G. M. Segers-Nolten, C. Otto, F. F. de Mul, and J. Greve, "Laser irradiation and Raman spectroscopy of single living cells and chromosomes: sample degradation occurs with 514.5 nm but not with 660 nm laser light," Exp. Cell Res. 195,361-367 (1991).
[CrossRef] [PubMed]

1990

G. J. Puppels, F. F. M. De Mul, C. Otto, J. Greve, M. Robert-Nicoud, D. J. Arndt-Jovin, and T. M. Jovin, "Studying single living cells and chromosomes by confocal Raman microspectroscopy," Nature (London, United Kingdom) 347,301-303 (1990).
[CrossRef] [PubMed]

Akimov, D. A.

S. O. Konorov, D. A. Akimov, E. E. Serebryannikov, A. A. Ivanov, M. V. Alfimov, and A. M. Zheltikov, "Cross-correlation frequency-resolved optical gating coherent anti-Stokes Raman scattering with frequency-converting photonic-crystal fibers," Phys. Rev. E 70,057601 (2004).
[CrossRef]

Alfimov, M. V.

S. O. Konorov, D. A. Akimov, E. E. Serebryannikov, A. A. Ivanov, M. V. Alfimov, and A. M. Zheltikov, "Cross-correlation frequency-resolved optical gating coherent anti-Stokes Raman scattering with frequency-converting photonic-crystal fibers," Phys. Rev. E 70,057601 (2004).
[CrossRef]

Araki, T.

Arndt-Jovin, D. J.

G. J. Puppels, F. F. M. De Mul, C. Otto, J. Greve, M. Robert-Nicoud, D. J. Arndt-Jovin, and T. M. Jovin, "Studying single living cells and chromosomes by confocal Raman microspectroscopy," Nature (London, United Kingdom) 347,301-303 (1990).
[CrossRef] [PubMed]

Boer, V. O.

Book, L. D.

J.-X. Cheng, A. Volkmer, L. D. Book, and X. S. Xie, "Multiplex coherent anti-stokes raman scattering microspectroscopy and study of lipid vesicles," J. Phys. Chem. B 106,8493-8498 (2002).
[CrossRef]

Caster, A. G.

S.-H. Lim, A. G. Caster, and S. R. Leone, "Single-pulse phase-control interferometric coherent anti-Stokes Raman scattering spectroscopy," Phys. Rev. A 30,2805-2807 (2005).

Cheng, J.-X.

H. Wang, Y. Fu, P. Zickmund, R. Shi, and J.-X. Cheng, "Coherent anti-Stokes Raman scattering imaging of live spinal tissues," Biophys. J. 89,581-591 (2005).

J.-X. Cheng, A. Volkmer, L. D. Book, and X. S. Xie, "Multiplex coherent anti-stokes raman scattering microspectroscopy and study of lipid vesicles," J. Phys. Chem. B 106,8493-8498 (2002).
[CrossRef]

J.-X. Cheng, Y. K. Jia, G. Zheng, and X. S. Xie, "Laser-scanning coherent anti-Stokes Raman scattering microscopy and applications to cell biology," Biophys. J. 83,502-509 (2002).
[CrossRef] [PubMed]

Cicerone, M. T.

de Mul, F. F.

G. J. Puppels, J. H. Olminkhof, G. M. Segers-Nolten, C. Otto, F. F. de Mul, and J. Greve, "Laser irradiation and Raman spectroscopy of single living cells and chromosomes: sample degradation occurs with 514.5 nm but not with 660 nm laser light," Exp. Cell Res. 195,361-367 (1991).
[CrossRef] [PubMed]

De Mul, F. F. M.

G. J. Puppels, F. F. M. De Mul, C. Otto, J. Greve, M. Robert-Nicoud, D. J. Arndt-Jovin, and T. M. Jovin, "Studying single living cells and chromosomes by confocal Raman microspectroscopy," Nature (London, United Kingdom) 347,301-303 (1990).
[CrossRef] [PubMed]

Dudovich, N.

D. Oron, N. Dudovich, and Y. Silberberg, "Single-pulse phase-contrast nonlinear Raman Spectroscopy," Phys. Rev. Lett. 89,273001 (2002).
[CrossRef]

D. Oron, N. Dudovich, D. Yelin, and Y. Silberberg, "Narrow-band coherent anti-stokes Raman signals from broad-band pulses," Phys. Rev. Lett. 88,063004 (2002).
[CrossRef] [PubMed]

Fu, Y.

H. Wang, Y. Fu, P. Zickmund, R. Shi, and J.-X. Cheng, "Coherent anti-Stokes Raman scattering imaging of live spinal tissues," Biophys. J. 89,581-591 (2005).

Fukui, K.

K. Isobe, W. Watanabe, S. Matsunaga, T. Higashi, K. Fukui, and K. Itoh, "Multi-spectral two-photon excited fluorescence microscopy using supercontinuum light source," Jpn. J. Appl. Phys. Part 2 44,L167-L169 (2005).
[CrossRef] [PubMed]

Gerritsen, H. C.

Greve, J.

C. Otto, A. Voroshilov, S. G. Kruglik, and J. Greve, "Vibrational bands of luminescent zinc(II)-octaethyl-porphyrin using a polarization-sensitive "microscopic" multiplex CARS technique," J. Raman Spectrosc. 32,495-501 (2001).
[CrossRef]

C. Otto, N. M. Sijtsema, and J. Greve, "Confocal Raman microspectroscopy of the activation of single neutrophilic granulocytes," Eur. Biophys. J. 27,582-589 (1998).
[CrossRef] [PubMed]

G. J. Puppels, J. H. Olminkhof, G. M. Segers-Nolten, C. Otto, F. F. de Mul, and J. Greve, "Laser irradiation and Raman spectroscopy of single living cells and chromosomes: sample degradation occurs with 514.5 nm but not with 660 nm laser light," Exp. Cell Res. 195,361-367 (1991).
[CrossRef] [PubMed]

G. J. Puppels, F. F. M. De Mul, C. Otto, J. Greve, M. Robert-Nicoud, D. J. Arndt-Jovin, and T. M. Jovin, "Studying single living cells and chromosomes by confocal Raman microspectroscopy," Nature (London, United Kingdom) 347,301-303 (1990).
[CrossRef] [PubMed]

Haber, L. H.

R. D. Schaller, J. Ziegelbauer, L. F. Lee, L. H. Haber, and R. J. Saykally, "Chemically selective imaging of subcellular structure in human hepatocytes with coherent anti-stokes Raman scattering (CARS) near-field scanning optical microscopy (NSOM)," J. Phys. Chem. B 106,8489-8492 (2002).
[CrossRef]

Hamaguchi, H.

Y.-S. Huang, T. Karashima, M. Yamamoto, and H. Hamaguchi, "Molecular-level investigation of the structure, transformation, and bioactivity of single living fission yeast cells by time- and space-resolved Raman Spectroscopy," Biochemistry 44,10009-10019 (2005).
[CrossRef] [PubMed]

H. Kano, and H. Hamaguchi, "Vibrationally resonant imaging of a single living cell by supercontinuum-based multiplex coherent anti-Stokes Raman scattering microspectroscopy," Opt. Express 13,1322-1327 (2005). http://www.opticsexpress.org/abstract.cfm?URI=OPEX-13-4-1322
[CrossRef] [PubMed]

H. Kano, and H. Hamaguchi, "Ultrabroadband (>2500 cm-1) Multiplex coherent anti-stokes raman scattering microspectroscopy using a supercontinuum generated from a photonic crystal fiber," Appl. Phys. Lett. 86,121113-121115 (2005).
[CrossRef] [PubMed]

Y.-S. Huang, T. Karashima, M. Yamamoto, T. Ogura, and H. Hamaguchi, "Raman spectroscopic signature of life in a living yeast cell," J. Raman Spectrosc. 35,525-526 (2004).
[CrossRef]

Y.-S. Huang, T. Karashima, M. Yamamoto, and H. Hamaguchi, "Molecular-level pursuit of yeast mitosis by time- and space-resolved Raman spectroscopy," J. Raman Spectrosc. 34,1-3 (2003).
[CrossRef]

H. Kano, and H. Hamaguchi, "Dispersion-compensated supercontinuum generation for ultrabroadband multiplex coherent anti-Stokes Raman scattering spectroscopy," J. Raman Spectrosc., in press.

Hamaguchi, H.-o.

Y. Naito, A. Toh-e,and H.-o. Hamaguchi, "In vivo time-resolved Raman imaging of a spontaneous death process of a single budding yeast cell," J. Raman Spectrosc. 36,837-839 (2005).
[CrossRef]

Hashimoto, M.

T. Ichimura, N. Hayazawa, M. Hashimoto, Y. Inouye, and S. Kawata, "Tip-enhanced coherent anti-stokes Raman scattering for vibrational nanoimaging," Phys. Rev. Lett. 92,220801 (2004).
[CrossRef] [PubMed]

M. Hashimoto, T. Araki, and S. Kawata, "Molecular vibration imaging in the fingerprint region by use of coherent anti-Stokes Raman scattering microscopy with a collinear configuration," Opt. Lett. 25,1768-1770 (2000).
[CrossRef]

Hayazawa, N.

T. Ichimura, N. Hayazawa, M. Hashimoto, Y. Inouye, and S. Kawata, "Tip-enhanced coherent anti-stokes Raman scattering for vibrational nanoimaging," Phys. Rev. Lett. 92,220801 (2004).
[CrossRef] [PubMed]

Higashi, T.

K. Isobe, W. Watanabe, S. Matsunaga, T. Higashi, K. Fukui, and K. Itoh, "Multi-spectral two-photon excited fluorescence microscopy using supercontinuum light source," Jpn. J. Appl. Phys. Part 2 44,L167-L169 (2005).
[CrossRef] [PubMed]

Hilligsoe, K. M.

Holtom, G. R.

A. Zumbusch, G. R. Holtom, and X. S. Xie, "Three-dimensional vibrational imaging by coherent anti-stokes raman scattering," Phys. Rev. Lett. 82,4142-4145 (1999).
[CrossRef]

Huang, Y.-S.

Y.-S. Huang, T. Karashima, M. Yamamoto, and H. Hamaguchi, "Molecular-level investigation of the structure, transformation, and bioactivity of single living fission yeast cells by time- and space-resolved Raman Spectroscopy," Biochemistry 44,10009-10019 (2005).
[CrossRef] [PubMed]

Y.-S. Huang, T. Karashima, M. Yamamoto, T. Ogura, and H. Hamaguchi, "Raman spectroscopic signature of life in a living yeast cell," J. Raman Spectrosc. 35,525-526 (2004).
[CrossRef]

Y.-S. Huang, T. Karashima, M. Yamamoto, and H. Hamaguchi, "Molecular-level pursuit of yeast mitosis by time- and space-resolved Raman spectroscopy," J. Raman Spectrosc. 34,1-3 (2003).
[CrossRef]

Ichimura, T.

T. Ichimura, N. Hayazawa, M. Hashimoto, Y. Inouye, and S. Kawata, "Tip-enhanced coherent anti-stokes Raman scattering for vibrational nanoimaging," Phys. Rev. Lett. 92,220801 (2004).
[CrossRef] [PubMed]

Inouye, Y.

T. Ichimura, N. Hayazawa, M. Hashimoto, Y. Inouye, and S. Kawata, "Tip-enhanced coherent anti-stokes Raman scattering for vibrational nanoimaging," Phys. Rev. Lett. 92,220801 (2004).
[CrossRef] [PubMed]

Isobe, K.

K. Isobe, W. Watanabe, S. Matsunaga, T. Higashi, K. Fukui, and K. Itoh, "Multi-spectral two-photon excited fluorescence microscopy using supercontinuum light source," Jpn. J. Appl. Phys. Part 2 44,L167-L169 (2005).
[CrossRef] [PubMed]

Itoh, K.

K. Isobe, W. Watanabe, S. Matsunaga, T. Higashi, K. Fukui, and K. Itoh, "Multi-spectral two-photon excited fluorescence microscopy using supercontinuum light source," Jpn. J. Appl. Phys. Part 2 44,L167-L169 (2005).
[CrossRef] [PubMed]

Ivanov, A. A.

S. O. Konorov, D. A. Akimov, E. E. Serebryannikov, A. A. Ivanov, M. V. Alfimov, and A. M. Zheltikov, "Cross-correlation frequency-resolved optical gating coherent anti-Stokes Raman scattering with frequency-converting photonic-crystal fibers," Phys. Rev. E 70,057601 (2004).
[CrossRef]

Jia, Y. K.

J.-X. Cheng, Y. K. Jia, G. Zheng, and X. S. Xie, "Laser-scanning coherent anti-Stokes Raman scattering microscopy and applications to cell biology," Biophys. J. 83,502-509 (2002).
[CrossRef] [PubMed]

Jovin, T. M.

G. J. Puppels, F. F. M. De Mul, C. Otto, J. Greve, M. Robert-Nicoud, D. J. Arndt-Jovin, and T. M. Jovin, "Studying single living cells and chromosomes by confocal Raman microspectroscopy," Nature (London, United Kingdom) 347,301-303 (1990).
[CrossRef] [PubMed]

Kano, H.

H. Kano, and H. Hamaguchi, "Ultrabroadband (>2500 cm-1) Multiplex coherent anti-stokes raman scattering microspectroscopy using a supercontinuum generated from a photonic crystal fiber," Appl. Phys. Lett. 86,121113-121115 (2005).
[CrossRef] [PubMed]

H. Kano, and H. Hamaguchi, "Vibrationally resonant imaging of a single living cell by supercontinuum-based multiplex coherent anti-Stokes Raman scattering microspectroscopy," Opt. Express 13,1322-1327 (2005). http://www.opticsexpress.org/abstract.cfm?URI=OPEX-13-4-1322
[CrossRef] [PubMed]

H. Kano, and H. Hamaguchi, "Dispersion-compensated supercontinuum generation for ultrabroadband multiplex coherent anti-Stokes Raman scattering spectroscopy," J. Raman Spectrosc., in press.

Karashima, T.

Y.-S. Huang, T. Karashima, M. Yamamoto, and H. Hamaguchi, "Molecular-level investigation of the structure, transformation, and bioactivity of single living fission yeast cells by time- and space-resolved Raman Spectroscopy," Biochemistry 44,10009-10019 (2005).
[CrossRef] [PubMed]

Y.-S. Huang, T. Karashima, M. Yamamoto, T. Ogura, and H. Hamaguchi, "Raman spectroscopic signature of life in a living yeast cell," J. Raman Spectrosc. 35,525-526 (2004).
[CrossRef]

Y.-S. Huang, T. Karashima, M. Yamamoto, and H. Hamaguchi, "Molecular-level pursuit of yeast mitosis by time- and space-resolved Raman spectroscopy," J. Raman Spectrosc. 34,1-3 (2003).
[CrossRef]

Kawata, S.

T. Ichimura, N. Hayazawa, M. Hashimoto, Y. Inouye, and S. Kawata, "Tip-enhanced coherent anti-stokes Raman scattering for vibrational nanoimaging," Phys. Rev. Lett. 92,220801 (2004).
[CrossRef] [PubMed]

M. Hashimoto, T. Araki, and S. Kawata, "Molecular vibration imaging in the fingerprint region by use of coherent anti-Stokes Raman scattering microscopy with a collinear configuration," Opt. Lett. 25,1768-1770 (2000).
[CrossRef]

Kee, T. W.

Keiding, S. R.

Konorov, S. O.

S. O. Konorov, D. A. Akimov, E. E. Serebryannikov, A. A. Ivanov, M. V. Alfimov, and A. M. Zheltikov, "Cross-correlation frequency-resolved optical gating coherent anti-Stokes Raman scattering with frequency-converting photonic-crystal fibers," Phys. Rev. E 70,057601 (2004).
[CrossRef]

Kruglik, S. G.

C. Otto, A. Voroshilov, S. G. Kruglik, and J. Greve, "Vibrational bands of luminescent zinc(II)-octaethyl-porphyrin using a polarization-sensitive "microscopic" multiplex CARS technique," J. Raman Spectrosc. 32,495-501 (2001).
[CrossRef]

Larsen, J. J.

Lee, L. F.

R. D. Schaller, J. Ziegelbauer, L. F. Lee, L. H. Haber, and R. J. Saykally, "Chemically selective imaging of subcellular structure in human hepatocytes with coherent anti-stokes Raman scattering (CARS) near-field scanning optical microscopy (NSOM)," J. Phys. Chem. B 106,8489-8492 (2002).
[CrossRef]

Leone, S. R.

S.-H. Lim, A. G. Caster, and S. R. Leone, "Single-pulse phase-control interferometric coherent anti-Stokes Raman scattering spectroscopy," Phys. Rev. A 30,2805-2807 (2005).

Lim, S.-H.

S.-H. Lim, A. G. Caster, and S. R. Leone, "Single-pulse phase-control interferometric coherent anti-Stokes Raman scattering spectroscopy," Phys. Rev. A 30,2805-2807 (2005).

Masuko, T.

Y. Takai, T. Masuko, and H. Takeuchi, "Lipid structure of cytotoxic granules in living human killer T lymphocytes studied by Raman microspectroscopy," Biochim. Biophys. Acta 1335,199-208 (1997).
[CrossRef] [PubMed]

Matsunaga, S.

K. Isobe, W. Watanabe, S. Matsunaga, T. Higashi, K. Fukui, and K. Itoh, "Multi-spectral two-photon excited fluorescence microscopy using supercontinuum light source," Jpn. J. Appl. Phys. Part 2 44,L167-L169 (2005).
[CrossRef] [PubMed]

McConnell, C.

C. McConnell, and E. Riis, "Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with fura-2," Phys. Med. Biol. 49,4757-4763 (2004).
[CrossRef]

Mueller, M.

Naito, Y.

Y. Naito, A. Toh-e,and H.-o. Hamaguchi, "In vivo time-resolved Raman imaging of a spontaneous death process of a single budding yeast cell," J. Raman Spectrosc. 36,837-839 (2005).
[CrossRef]

Ogura, T.

Y.-S. Huang, T. Karashima, M. Yamamoto, T. Ogura, and H. Hamaguchi, "Raman spectroscopic signature of life in a living yeast cell," J. Raman Spectrosc. 35,525-526 (2004).
[CrossRef]

Olminkhof, J. H.

G. J. Puppels, J. H. Olminkhof, G. M. Segers-Nolten, C. Otto, F. F. de Mul, and J. Greve, "Laser irradiation and Raman spectroscopy of single living cells and chromosomes: sample degradation occurs with 514.5 nm but not with 660 nm laser light," Exp. Cell Res. 195,361-367 (1991).
[CrossRef] [PubMed]

Oron, D.

D. Oron, N. Dudovich, and Y. Silberberg, "Single-pulse phase-contrast nonlinear Raman Spectroscopy," Phys. Rev. Lett. 89,273001 (2002).
[CrossRef]

D. Oron, N. Dudovich, D. Yelin, and Y. Silberberg, "Narrow-band coherent anti-stokes Raman signals from broad-band pulses," Phys. Rev. Lett. 88,063004 (2002).
[CrossRef] [PubMed]

Otto, C.

C. Otto, A. Voroshilov, S. G. Kruglik, and J. Greve, "Vibrational bands of luminescent zinc(II)-octaethyl-porphyrin using a polarization-sensitive "microscopic" multiplex CARS technique," J. Raman Spectrosc. 32,495-501 (2001).
[CrossRef]

C. Otto, N. M. Sijtsema, and J. Greve, "Confocal Raman microspectroscopy of the activation of single neutrophilic granulocytes," Eur. Biophys. J. 27,582-589 (1998).
[CrossRef] [PubMed]

G. J. Puppels, J. H. Olminkhof, G. M. Segers-Nolten, C. Otto, F. F. de Mul, and J. Greve, "Laser irradiation and Raman spectroscopy of single living cells and chromosomes: sample degradation occurs with 514.5 nm but not with 660 nm laser light," Exp. Cell Res. 195,361-367 (1991).
[CrossRef] [PubMed]

G. J. Puppels, F. F. M. De Mul, C. Otto, J. Greve, M. Robert-Nicoud, D. J. Arndt-Jovin, and T. M. Jovin, "Studying single living cells and chromosomes by confocal Raman microspectroscopy," Nature (London, United Kingdom) 347,301-303 (1990).
[CrossRef] [PubMed]

Palero, J. A.

Paulsen, H. N.

Petrov, I. G.

Puppels, G. J.

G. J. Puppels, J. H. Olminkhof, G. M. Segers-Nolten, C. Otto, F. F. de Mul, and J. Greve, "Laser irradiation and Raman spectroscopy of single living cells and chromosomes: sample degradation occurs with 514.5 nm but not with 660 nm laser light," Exp. Cell Res. 195,361-367 (1991).
[CrossRef] [PubMed]

G. J. Puppels, F. F. M. De Mul, C. Otto, J. Greve, M. Robert-Nicoud, D. J. Arndt-Jovin, and T. M. Jovin, "Studying single living cells and chromosomes by confocal Raman microspectroscopy," Nature (London, United Kingdom) 347,301-303 (1990).
[CrossRef] [PubMed]

Riis, E.

C. McConnell, and E. Riis, "Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with fura-2," Phys. Med. Biol. 49,4757-4763 (2004).
[CrossRef]

Robert-Nicoud, M.

G. J. Puppels, F. F. M. De Mul, C. Otto, J. Greve, M. Robert-Nicoud, D. J. Arndt-Jovin, and T. M. Jovin, "Studying single living cells and chromosomes by confocal Raman microspectroscopy," Nature (London, United Kingdom) 347,301-303 (1990).
[CrossRef] [PubMed]

Saykally, R. J.

R. D. Schaller, J. Ziegelbauer, L. F. Lee, L. H. Haber, and R. J. Saykally, "Chemically selective imaging of subcellular structure in human hepatocytes with coherent anti-stokes Raman scattering (CARS) near-field scanning optical microscopy (NSOM)," J. Phys. Chem. B 106,8489-8492 (2002).
[CrossRef]

Schaller, R. D.

R. D. Schaller, J. Ziegelbauer, L. F. Lee, L. H. Haber, and R. J. Saykally, "Chemically selective imaging of subcellular structure in human hepatocytes with coherent anti-stokes Raman scattering (CARS) near-field scanning optical microscopy (NSOM)," J. Phys. Chem. B 106,8489-8492 (2002).
[CrossRef]

Schins, J. M.

Segers-Nolten, G. M.

G. J. Puppels, J. H. Olminkhof, G. M. Segers-Nolten, C. Otto, F. F. de Mul, and J. Greve, "Laser irradiation and Raman spectroscopy of single living cells and chromosomes: sample degradation occurs with 514.5 nm but not with 660 nm laser light," Exp. Cell Res. 195,361-367 (1991).
[CrossRef] [PubMed]

Serebryannikov, E. E.

S. O. Konorov, D. A. Akimov, E. E. Serebryannikov, A. A. Ivanov, M. V. Alfimov, and A. M. Zheltikov, "Cross-correlation frequency-resolved optical gating coherent anti-Stokes Raman scattering with frequency-converting photonic-crystal fibers," Phys. Rev. E 70,057601 (2004).
[CrossRef]

Shi, R.

H. Wang, Y. Fu, P. Zickmund, R. Shi, and J.-X. Cheng, "Coherent anti-Stokes Raman scattering imaging of live spinal tissues," Biophys. J. 89,581-591 (2005).

Sijtsema, N. M.

C. Otto, N. M. Sijtsema, and J. Greve, "Confocal Raman microspectroscopy of the activation of single neutrophilic granulocytes," Eur. Biophys. J. 27,582-589 (1998).
[CrossRef] [PubMed]

Silberberg, Y.

D. Oron, N. Dudovich, D. Yelin, and Y. Silberberg, "Narrow-band coherent anti-stokes Raman signals from broad-band pulses," Phys. Rev. Lett. 88,063004 (2002).
[CrossRef] [PubMed]

D. Oron, N. Dudovich, and Y. Silberberg, "Single-pulse phase-contrast nonlinear Raman Spectroscopy," Phys. Rev. Lett. 89,273001 (2002).
[CrossRef]

Sterenborg, H. J. C. M.

Takai, Y.

Y. Takai, T. Masuko, and H. Takeuchi, "Lipid structure of cytotoxic granules in living human killer T lymphocytes studied by Raman microspectroscopy," Biochim. Biophys. Acta 1335,199-208 (1997).
[CrossRef] [PubMed]

Takeuchi, H.

Y. Takai, T. Masuko, and H. Takeuchi, "Lipid structure of cytotoxic granules in living human killer T lymphocytes studied by Raman microspectroscopy," Biochim. Biophys. Acta 1335,199-208 (1997).
[CrossRef] [PubMed]

Thogersen, J.

Toh-e, A.

Y. Naito, A. Toh-e,and H.-o. Hamaguchi, "In vivo time-resolved Raman imaging of a spontaneous death process of a single budding yeast cell," J. Raman Spectrosc. 36,837-839 (2005).
[CrossRef]

Vijverberg, J. C.

Volkmer, A.

J.-X. Cheng, A. Volkmer, L. D. Book, and X. S. Xie, "Multiplex coherent anti-stokes raman scattering microspectroscopy and study of lipid vesicles," J. Phys. Chem. B 106,8493-8498 (2002).
[CrossRef]

Voroshilov, A.

C. Otto, A. Voroshilov, S. G. Kruglik, and J. Greve, "Vibrational bands of luminescent zinc(II)-octaethyl-porphyrin using a polarization-sensitive "microscopic" multiplex CARS technique," J. Raman Spectrosc. 32,495-501 (2001).
[CrossRef]

Wang, H.

H. Wang, Y. Fu, P. Zickmund, R. Shi, and J.-X. Cheng, "Coherent anti-Stokes Raman scattering imaging of live spinal tissues," Biophys. J. 89,581-591 (2005).

Watanabe, W.

K. Isobe, W. Watanabe, S. Matsunaga, T. Higashi, K. Fukui, and K. Itoh, "Multi-spectral two-photon excited fluorescence microscopy using supercontinuum light source," Jpn. J. Appl. Phys. Part 2 44,L167-L169 (2005).
[CrossRef] [PubMed]

Wurpel, G. W. H.

Xie, X. S.

J.-X. Cheng, Y. K. Jia, G. Zheng, and X. S. Xie, "Laser-scanning coherent anti-Stokes Raman scattering microscopy and applications to cell biology," Biophys. J. 83,502-509 (2002).
[CrossRef] [PubMed]

J.-X. Cheng, A. Volkmer, L. D. Book, and X. S. Xie, "Multiplex coherent anti-stokes raman scattering microspectroscopy and study of lipid vesicles," J. Phys. Chem. B 106,8493-8498 (2002).
[CrossRef]

A. Zumbusch, G. R. Holtom, and X. S. Xie, "Three-dimensional vibrational imaging by coherent anti-stokes raman scattering," Phys. Rev. Lett. 82,4142-4145 (1999).
[CrossRef]

Yakovlev, V. V.

Yamamoto, M.

Y.-S. Huang, T. Karashima, M. Yamamoto, and H. Hamaguchi, "Molecular-level investigation of the structure, transformation, and bioactivity of single living fission yeast cells by time- and space-resolved Raman Spectroscopy," Biochemistry 44,10009-10019 (2005).
[CrossRef] [PubMed]

Y.-S. Huang, T. Karashima, M. Yamamoto, T. Ogura, and H. Hamaguchi, "Raman spectroscopic signature of life in a living yeast cell," J. Raman Spectrosc. 35,525-526 (2004).
[CrossRef]

Y.-S. Huang, T. Karashima, M. Yamamoto, and H. Hamaguchi, "Molecular-level pursuit of yeast mitosis by time- and space-resolved Raman spectroscopy," J. Raman Spectrosc. 34,1-3 (2003).
[CrossRef]

Yelin, D.

D. Oron, N. Dudovich, D. Yelin, and Y. Silberberg, "Narrow-band coherent anti-stokes Raman signals from broad-band pulses," Phys. Rev. Lett. 88,063004 (2002).
[CrossRef] [PubMed]

Zheltikov, A. M.

S. O. Konorov, D. A. Akimov, E. E. Serebryannikov, A. A. Ivanov, M. V. Alfimov, and A. M. Zheltikov, "Cross-correlation frequency-resolved optical gating coherent anti-Stokes Raman scattering with frequency-converting photonic-crystal fibers," Phys. Rev. E 70,057601 (2004).
[CrossRef]

Zheng, G.

J.-X. Cheng, Y. K. Jia, G. Zheng, and X. S. Xie, "Laser-scanning coherent anti-Stokes Raman scattering microscopy and applications to cell biology," Biophys. J. 83,502-509 (2002).
[CrossRef] [PubMed]

Zickmund, P.

H. Wang, Y. Fu, P. Zickmund, R. Shi, and J.-X. Cheng, "Coherent anti-Stokes Raman scattering imaging of live spinal tissues," Biophys. J. 89,581-591 (2005).

Ziegelbauer, J.

R. D. Schaller, J. Ziegelbauer, L. F. Lee, L. H. Haber, and R. J. Saykally, "Chemically selective imaging of subcellular structure in human hepatocytes with coherent anti-stokes Raman scattering (CARS) near-field scanning optical microscopy (NSOM)," J. Phys. Chem. B 106,8489-8492 (2002).
[CrossRef]

Zumbusch, A.

A. Zumbusch, G. R. Holtom, and X. S. Xie, "Three-dimensional vibrational imaging by coherent anti-stokes raman scattering," Phys. Rev. Lett. 82,4142-4145 (1999).
[CrossRef]

Appl. Phys. Lett.

H. Kano, and H. Hamaguchi, "Ultrabroadband (>2500 cm-1) Multiplex coherent anti-stokes raman scattering microspectroscopy using a supercontinuum generated from a photonic crystal fiber," Appl. Phys. Lett. 86,121113-121115 (2005).
[CrossRef] [PubMed]

Biochemistry

Y.-S. Huang, T. Karashima, M. Yamamoto, and H. Hamaguchi, "Molecular-level investigation of the structure, transformation, and bioactivity of single living fission yeast cells by time- and space-resolved Raman Spectroscopy," Biochemistry 44,10009-10019 (2005).
[CrossRef] [PubMed]

Biochim. Biophys. Acta

Y. Takai, T. Masuko, and H. Takeuchi, "Lipid structure of cytotoxic granules in living human killer T lymphocytes studied by Raman microspectroscopy," Biochim. Biophys. Acta 1335,199-208 (1997).
[CrossRef] [PubMed]

Biophys. J.

J.-X. Cheng, Y. K. Jia, G. Zheng, and X. S. Xie, "Laser-scanning coherent anti-Stokes Raman scattering microscopy and applications to cell biology," Biophys. J. 83,502-509 (2002).
[CrossRef] [PubMed]

H. Wang, Y. Fu, P. Zickmund, R. Shi, and J.-X. Cheng, "Coherent anti-Stokes Raman scattering imaging of live spinal tissues," Biophys. J. 89,581-591 (2005).

Eur. Biophys. J.

C. Otto, N. M. Sijtsema, and J. Greve, "Confocal Raman microspectroscopy of the activation of single neutrophilic granulocytes," Eur. Biophys. J. 27,582-589 (1998).
[CrossRef] [PubMed]

Exp. Cell Res.

G. J. Puppels, J. H. Olminkhof, G. M. Segers-Nolten, C. Otto, F. F. de Mul, and J. Greve, "Laser irradiation and Raman spectroscopy of single living cells and chromosomes: sample degradation occurs with 514.5 nm but not with 660 nm laser light," Exp. Cell Res. 195,361-367 (1991).
[CrossRef] [PubMed]

J. Phys. Chem. B

R. D. Schaller, J. Ziegelbauer, L. F. Lee, L. H. Haber, and R. J. Saykally, "Chemically selective imaging of subcellular structure in human hepatocytes with coherent anti-stokes Raman scattering (CARS) near-field scanning optical microscopy (NSOM)," J. Phys. Chem. B 106,8489-8492 (2002).
[CrossRef]

J.-X. Cheng, A. Volkmer, L. D. Book, and X. S. Xie, "Multiplex coherent anti-stokes raman scattering microspectroscopy and study of lipid vesicles," J. Phys. Chem. B 106,8493-8498 (2002).
[CrossRef]

J. Raman Spectrosc.

Y. Naito, A. Toh-e,and H.-o. Hamaguchi, "In vivo time-resolved Raman imaging of a spontaneous death process of a single budding yeast cell," J. Raman Spectrosc. 36,837-839 (2005).
[CrossRef]

Y.-S. Huang, T. Karashima, M. Yamamoto, and H. Hamaguchi, "Molecular-level pursuit of yeast mitosis by time- and space-resolved Raman spectroscopy," J. Raman Spectrosc. 34,1-3 (2003).
[CrossRef]

Y.-S. Huang, T. Karashima, M. Yamamoto, T. Ogura, and H. Hamaguchi, "Raman spectroscopic signature of life in a living yeast cell," J. Raman Spectrosc. 35,525-526 (2004).
[CrossRef]

C. Otto, A. Voroshilov, S. G. Kruglik, and J. Greve, "Vibrational bands of luminescent zinc(II)-octaethyl-porphyrin using a polarization-sensitive "microscopic" multiplex CARS technique," J. Raman Spectrosc. 32,495-501 (2001).
[CrossRef]

H. Kano, and H. Hamaguchi, "Dispersion-compensated supercontinuum generation for ultrabroadband multiplex coherent anti-Stokes Raman scattering spectroscopy," J. Raman Spectrosc., in press.

Jpn. J. Appl. Phys

K. Isobe, W. Watanabe, S. Matsunaga, T. Higashi, K. Fukui, and K. Itoh, "Multi-spectral two-photon excited fluorescence microscopy using supercontinuum light source," Jpn. J. Appl. Phys. Part 2 44,L167-L169 (2005).
[CrossRef] [PubMed]

Nature (London, United Kingdom)

G. J. Puppels, F. F. M. De Mul, C. Otto, J. Greve, M. Robert-Nicoud, D. J. Arndt-Jovin, and T. M. Jovin, "Studying single living cells and chromosomes by confocal Raman microspectroscopy," Nature (London, United Kingdom) 347,301-303 (1990).
[CrossRef] [PubMed]

Opt. Express

Opt. Lett.

Phys. Med. Biol.

C. McConnell, and E. Riis, "Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with fura-2," Phys. Med. Biol. 49,4757-4763 (2004).
[CrossRef]

Phys. Rev. A

S.-H. Lim, A. G. Caster, and S. R. Leone, "Single-pulse phase-control interferometric coherent anti-Stokes Raman scattering spectroscopy," Phys. Rev. A 30,2805-2807 (2005).

Phys. Rev. E

S. O. Konorov, D. A. Akimov, E. E. Serebryannikov, A. A. Ivanov, M. V. Alfimov, and A. M. Zheltikov, "Cross-correlation frequency-resolved optical gating coherent anti-Stokes Raman scattering with frequency-converting photonic-crystal fibers," Phys. Rev. E 70,057601 (2004).
[CrossRef]

Phys. Rev. Lett.

D. Oron, N. Dudovich, and Y. Silberberg, "Single-pulse phase-contrast nonlinear Raman Spectroscopy," Phys. Rev. Lett. 89,273001 (2002).
[CrossRef]

D. Oron, N. Dudovich, D. Yelin, and Y. Silberberg, "Narrow-band coherent anti-stokes Raman signals from broad-band pulses," Phys. Rev. Lett. 88,063004 (2002).
[CrossRef] [PubMed]

T. Ichimura, N. Hayazawa, M. Hashimoto, Y. Inouye, and S. Kawata, "Tip-enhanced coherent anti-stokes Raman scattering for vibrational nanoimaging," Phys. Rev. Lett. 92,220801 (2004).
[CrossRef] [PubMed]

A. Zumbusch, G. R. Holtom, and X. S. Xie, "Three-dimensional vibrational imaging by coherent anti-stokes raman scattering," Phys. Rev. Lett. 82,4142-4145 (1999).
[CrossRef]

Other

http://www.opticsexpress.org/abstract.cfm?URI=OPEX-13-14-5363.
[CrossRef] [PubMed]

H. Kano, and H. Hamaguchi, "Vibrational imaging of a J-aggregate microcrystal using ultrabroadband multiplex coherent anti-Stokes Raman scattering microspectroscopy," submitted toVibrational Spectroscopy.

http://www.opticsexpress.org/abstract.cfm?URI=OPEX-13-4-1299
[CrossRef]

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Figures (4)

Fig. 1.
Fig. 1.

(a) Spectral profiles of the CARS signal of a living yeast cell (red) and surrounding water (blue) obtained in 100 ms exposure time; (b) CARS image of living yeast cell at a Raman shift of 2840 cm-1. The CARS signals at the positions of black and white crosses correspond to the red and blue curves in Fig. 1(a), respectively.

Fig. 2.
Fig. 2.

Lateral(a) and axial(b) CARS images of a yeast cell at a Raman shift of 2840 cm-1; (c) Axial slices of the yeast cell at different depth positions, which are indicated at the top in a micrometer scale.

Fig. 3.
Fig. 3.

Spectral profile of the CARS and TPEF signals of a living yeast cell; CARS lateral images of living yeast cells at the Raman shift of 2840 cm-1 at the delay time of zero (b) and -4 ps (c), respectively; TPEF lateral images of the same system at 506 nm at the delay time of zero (d) and -4 ps (e), respectively; TPEF image only due to the non-degenerated (ω12)- photon process.

Fig. 4.
Fig. 4.

In-vivo multi-nonlinear optical imaging of yeast cells. The CARS and TPEF images are indicated as red and green, respectively.

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