Abstract

We demonstrate an optical method for edge contrast enhancement in light microscopy. The method is based on holographic Fourier plane filtering of the microscopic image with a spiral phase element (also called vortex phase or helical phase filter) displayed as an off-axis hologram at a computer controlled high resolution spatial light modulator (SLM) in the optical imaging pathway. The phase hologram imprints a helical phase term of the form exp(i �?) on the diffracted light field in its Fourier plane. In the image plane, this results in a strong and isotropic edge contrast enhancement for both amplitude and phase objects.

© 2005 Optical Society of America

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Appl. Opt. (2)

J. Microscopy (2)

A. Y. M. NG, C. W. See, and M. G. Somekh, �??Quantitative optical microscope with enhanced resolution using a pixelated liquid crystal spatial light modulator,�?? J. Microscopy 214, 334�??340 (2004)

M. R. Arnison, K. G. Larkin, C. J. R. Sheppard, N. I. Smith, and C. J. Cogswell, �??Linear phase imaging using differential interference contrast microscopy,�?? J. Microscopy 214, 7�??12 (2004)

J. Opt. Soc. Am. A (1)

Opt. Express (1)

Opt. Lett. (3)

Progress in Optics (1)

R. Oron, N. Davidson, A. A. Friesem, and E. Hasman, �??Transverse mode shaping and selection in laser resonators,�?? Progress in Optics 42, 325�??386 (2001)

Other (1)

G. O. Reynolds, J. B. DeVelis, G. B. Parrent, Jr., and B. J. Thompson, The new physical optics notebook: Tutorials in Fourier optics (SPIE Optical Engineering Press, Bellingham, Washington, 1989)

Supplementary Material (1)

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