Abstract

In microscopy, high magnifications are achievable for investigating micro-objects but the paradigm is that higher is the required magnification, lower is the depth of focus. For an object having a three-dimensional (3D) complex shape only a portion of it appears in good focus to the observer who is essentially looking at a single image plane. Actually, two approaches exist to obtain an extended focused image, both having severe limitations since the first requires mechanical scanning while the other one requires specially designed optics. We demonstrate that an extended focused image of an object can be obtained through digital holography without any mechanical scanning or special optical components. The conceptual novelty of the proposed approach lies in the fact that it is possible to completely exploit the unique feature of DH in extracting all the information content stored in hologram, amplitude and phase, to extend the depth of focus.

© 2005 Optical Society of America

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References

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Appl. Opt. (4)

Appl. Phys. Lett. (1)

J.W. Goodman, R. W. Lawrence, �??Digital image formation from electronically detected holograms,�?? Appl. Phys. Lett. 11, 77-79 (1967).
[CrossRef]

Consultants Bureau, New York (1980) (1)

L. P. Yaroslavsky, N.S. Merzlyakov, �??Methods of digital holography,�?? Consultants Bureau, New York (1980).

Meas. Sci. Technol. (2)

G. Coppola, P. Ferraro, M. Iodice, S. De Nicola, A. Finizio, S. Grilli, �??A digital holographic microscope for complete characterization of microelectromechanical systems,�?? Meas. Sci. Technol. 15, 529-539, (2004).
[CrossRef]

U. Schnars, W.P.O. Juptner, �??Digital recording and numerical reconstruction of holograms,�?? Meas. Sci. Technol. 13, R85-R101 (2002).
[CrossRef]

Nat. Bio. (1)

S. E. Fraser, �??Crystal gazing in optical microscopy,�?? Nat. Bio. 21, 1272-1273 (2003).

Opt. Commun. (1)

G. Hausler, �??A method to increase the depth of focus by two step image processing,�?? Opt. Commun. 6, 38 (1972).
[CrossRef]

Opt. Express. (1)

S. Grilli, P. Ferraro, S. DeNicola, A. Finizio, G. Pierattini, R. Meucci, �??Whole optical wavefields reconstruction by digital holography,�?? Opt. Express. 9, 294-302 (2001).

Opt. Lett. (4)

Proc. Royal Society A 197 (1)

D. Gabor, �??Microscopy by reconstructed wave-fronts,�?? Proc. Royal Society A 197, 454-487 (1949).

Other (4)

For example, description of EFI capability and process in optical microscopes is into the web sites of two important manufacturers: <a href="http://www.olympusamerica.com/seg_section/msfive/ms5_appmod.asp">http://www.olympusamerica.com/seg_section/msfive/ms5_appmod.asp</a> and <a href="http://www.zeiss.de/C12567BE0045ACF1/InhaltFrame/DA8E39D74AA60C49412568B90054EDD2">http://www.zeiss.de/C12567BE0045ACF1/InhaltFrame/DA8E39D74AA60C49412568B90054EDD2</a>.

L. Mertz, �??Transformation in Optics,�?? 101 (Wiley, New York, 1965).

R. Hooke, �??Micrographia,�?? (Warnock Library, London, 1665).

D. L. Barton, et al �??Wavefront coded imaging system for MEMS analysis�??, Presented at international Society for testing and failure analysis meeting, Phoenix, AZ (USA) (Nov. 2002).

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