Abstract

We present a novel angle-resolved low coherence interferometry scheme for rapid measurement of depth-resolved angular scattering distributions to enable determination of scatterer size via elastic scattering properties. Depth resolution is achieved using a superluminescent diode in a modified Mach-Zehnder interferometer with the mixed signal and reference fields dispersed by an imaging spectrograph. The spectrograph slit is located in a Fourier transform plane of the scattering sample, enabling angle-resolved measurements over a 0.21 radian range. The capabilities of the new technique are demonstrated by recording the distribution of light scattered by a sub-surface layer of polystyrene microspheres in 40 milliseconds. The data are used to determine the microsphere size with good accuracy. Future clinical application to measuring the size of cell nuclei in living epithelial tissues using backscattered light is discussed.

© 2004 Optical Society of America

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AACR (1)

A. Wax, J.W. Pyhtila, R.N. Graf, R. Nines, C.W. Boone, R.R. Dasari, M.S. Feld, V.E. Steele, and G.D. Stoner, "In situ monitoring of neoplastic transformation and assessing efficacy of chemopreventive agents in rat esophagus epithelium using angle-resolved low-coherence interferometry," Proceedings of the AACR, Volume 45, (2004).

Biophys. J. (1)

A. Wax, C. Yang, V. Backman, K. Badizadegan, C.W. Boone, R.R. Dasari, and M.S. Feld, "Cell organization and sub-structure measured using angle-resolved low coherence interferometry.," Biophys. J. 82, 2256-2264 (2002).

Cancer Res. (1)

A. Wax, C.H. Yang, M.G. Muller, R. Nines, C.W. Boone, V.E. Steele, G.D. Stoner, R.R. Dasari, and M.S. Feld, "In situ detection of neoplastic transformation and chemopreventive effects in rat esophagus epithelium using angle-resolved low-coherence interferometry," Cancer Res. 63, 3556-3559 (2003).

Gastroenterology (4 ) (1)

H.K. Roy, Y. Liu, R.K. Wali, Y.L. Kim, A.K. Kromine, M.J. Goldberg, and V. Backman, "Four-dimensional elastic light-scattering fingerprints as preneoplastic markers in the rat model of colon carcinogenesis," Gastroenterology 126(4): p. 1071-1081 (2004).

IEEE J. Sel. Top. Quant. Elec. (1)

Y.L. Kim, Y. Liu, R.K. Wali, H.K. Roy, M.J. Goldberg, A.K. Kromin, K. Chen, and V. Backman, "Simultaneous measurement of angular and spectral properties of light scattering for characterization of tissue microarchitecture and its alteration in early precancer," IEEE J. Sel. Top. Quant. Elec. 9, 243-256 (2003).

J. Opt. Soc. Am. A (1)

Opt. Express (6)

J.W. Pyhtila, R.N. Graf, and A. Wax, "Determining nuclear morphology using an improved angle-resolved low coherence interferometry system," Opt. Express 11, 3473-3484 (2003). <a href= "http://www.opticsexpress.org/abstract.cfm?URI=OPEX-11-25-3473">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-11-25-3473</a>

R. Leitgeb, C.K. Hitzenberger, and A.F. Fercher, "Performance of fourier domain vs. time domain optical coherence tomography," Opt. Express 11, 889-894 (2003). <a href= "http://www.opticsexpress.org/abstract.cfm?URI=OPEX-11-8-889">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-11-8-889</a>

R.A. Leitgeb, W. Drexler, A. Unterhuber, B. Hermann, T. Bajraszewski, T. Le, A. Stingl, and A.F. Fercher, "Ultrahigh resolution Fourier domain optical coherence tomography," Opt. Express 12, 2156-2165 (2004). <a href= "http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-10-2156">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-10-2156</a>
[CrossRef]

N.A. Nassif, B. Cense, B.H. Park, M.C. Pierce, S.H. Yun, B.E. Bouma, G.J. Tearney, T.C. Chen, and J.F. de Boer, "In vivo high-resolution video-rate spectral-domain optical coherence tomography of the human retina and optic nerve," Opt. Express 12, 367-376 (2004). <a href= "http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-3-367">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-3-367</a>
[CrossRef]

M. Wojtkowski, V.J. Srinivasan, T.H. Ko, J.G. Fujimoto, A. Kowalczyk, and J.S. Duker, "Ultrahighresolution, high-speed, Fourier domain optical coherence tomography and methods for dispersion compensation," Opt. Express 12, 2404-2422 (2004), <a href= "http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-11-2404">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-11-2404</a>
[CrossRef]

M.A. Choma, M.V. Sarunic, C.H. Yang, and J.A. Izatt, "Sensitivity advantage of swept source and Fourier domain optical coherence tomography," Opt. Express 11, 2183-2189 (2003). <a href= "http://www.opticsexpress.org/abstract.cfm?URI=OPEX-11-18-2183">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-11-18-2183</a>

Opt. Lett. (2)

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