Abstract

Mie based theoretical analysis has been performed for investigation of the possibility of application of the plasma hemoglobin releasing due to local hemolysis for optical clearing of blood. The 30–40% reduction of the scattering coefficient of blood in the spectral range from 400 to 1000 nm with increase of degree of hemolysis (up to 20%) was shown. At the same time, the reduction of absorption coefficient of blood is localized mainly within the Soret band with maximum at 415 nm (~15%), the α-band at 540 nm and the β-band at 577 nm (~10%) of oxyhemoglobin. In the spectral range from 700 to 1000 nm the decrease of absorption coefficient is less than 8%.

© 2004 Optical Society of America

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References

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Appl. Opt. (3)

Circulation (1)

M. Brezinski, K. Saunders, C. Jesser, X. Li, J. Fujimoto, ???Index matching to improve optical coherence tomography imaging through blood,??? Circulation 10, 1999-2003 (2001).
[CrossRef]

J. Appl. Spectrosc. (1)

A.Ya. Khairullina and S.F. Shumilina, ???Determination of the distribution function of erythrocytes according to size by the spectral transparency method,??? J. Appl. Spectrosc. 19, 1078-1083 (1973).
[CrossRef]

J. Biomed. Opt. (2)

A.G. Borovoi, E.I. Naats, and U.G. Oppel,???Scattering of Light by a Red Blood Cell,??? J. Biomed. Opt. 3, 364-372 (1998).
[CrossRef] [PubMed]

A. Roggan, M. Friebel, K. Dorschel, A. Hahn, and G. Muller, ???Optical Properties of Circulating Human Blood in the Wavelength Range 400-2500 nm,??? J. Biomed. Opt. 4, 36-43 (1999).
[CrossRef] [PubMed]

J. Opt. Soc. Am. A (1)

Opt. Spectrosc. (1)

N.G. Khlebtsov, S.Yu. Shchyogolev, "Account of particle nonsphericity at determination of parameters of dispersion systems by a turbidity spectrum method. 1. Characteristic functions of light scattering by nonspherical particle systems in Rayleigh-Gans approximation," Opt. Spectrosc. 42, 956-962 (1977).

Phys. Med. Biol. (1)

X. Xu, R.K. Wang, J.B. Elder, V.V. Tuchin, ???Effect of dextran-induced changes in refractive index and aggregation on optical properties of whole blood,??? Phys. Med. Biol. 48, 1205-1221 (2003).
[CrossRef] [PubMed]

Proc. SPIE (2)

D.M. Zhestkov, A.N. Bashkatov, E.A. Genina, V.V. Tuchin, ???Influence of clearing solutions osmolarity on the optical properties of RBC,??? Proc. SPIE 5474, (2004 to be published).
[CrossRef]

S. Cheng, H.Y. Shen, G. Zhang, C.H. Huang, X.J. Huang, "Measurement of the refractive index of biotissue at four laser wavelengths," in Optics in Health Care and Biomedical Optics: Diagnostics and Treatment, B. Chance, M. Chen and G. Yoon , eds., Proc. SPIE 4916, 172-176 (2002).

Other (5)

C. Bohren, D. Huffman, Absorption and Scattering of Light by Small Particles (John Wiley & Sons, New York, 1983).

V.V. Tuchin, Tissue Optics: Light Scattering Methods and Instruments for Medical Diagnosis (SPIE Press, TT38, Bellingham, Washington, 2000).

V.V. Tuchin (ed.), Handbook of Optical Biomedical Diagnostics (PM107, SPIE Press, Bellingham, WA, 2002).

Tuan Vo-Dinh (ed.), Biomedical Photonics Handbook (CRC Press, Boca Raton, 2003).
[CrossRef]

B.E. Bouma and G.J. Tearney (eds.), Handbook of Optical Coherence Tomography (Marcel-Dekker, New York, 2002).

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Figures (3)

Fig. 1.
Fig. 1.

The absorption spectra at different degree of whole blood hemolysis.

Fig. 2.
Fig. 2.

The change of scattering spectrum at different degree of blood hemolysis

Fig. 3.
Fig. 3.

Scattering coefficient (red) and anisotropy factor (blue) versus the degree of blood hemolysis.

Tables (2)

Tables Icon

Table 1. The calculated values of local blood hematocrit and hemoglobin concentration in plasma at different degree of hemolysis.

Tables Icon

Table 2. Size distribution of spherical particles modeling RBC a

Equations (6)

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n Hb ( λ ) = n water ( λ ) + α C ,
χ Hb ( λ ) = β C ,
n p Hb ( λ ) = n pl ( λ ) + α C ,
osm pl = osm pl + C FHb M Hb ,
V ( osm ) = V 0 ( 0.515 + 1.177 exp ( osm 337 ) ) ,
m im = n e n p + χ e β C FHb n p 2 + β 2 C FHb 2 + i χ e n p n e β C FHb n p 2 + β 2 C FHb 2 ,

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