Abstract

We demonstrate real-time acquisition, processing, and display of tissue structure, birefringence, and blood flow in a multi-functional optical coherence tomography (MF-OCT) system. This is accomplished by efficient data processing of the phase-resolved inteference patterns without dedicated hardware or extensive modification to the high-speed fiber-based OCT system. The system acquires images of 2048 depth scans per second, covering an area of 5 mm in width �? 1.2 mm in depth with real-time display updating images in a rolling manner 32 times each second. We present a video of the system display as images from the proximal nail fold of a human volunteer are taken.

© 2003 Optical Society of America

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Appl. Opt. (1)

J. Biomed. Opt. (3)

D. Fried, J. Xie, S. Shafi, J.D.B. Featherstone, T.M. Breunig, C. Le, �??Imaging caries lesions and lesion progression with polarization sensitive optical coherence tomography,�?? J. Biomed. Opt. 7, 618-27 (2002).
[CrossRef]

A.M. Rollins, S. Yazdanfar, J.K. Barton, J.A. Izatt, �??Real-time in vivo color Doppler optical coherence tomography,�?? J. Biomed. Opt. 7, 123-9 (2002).
[CrossRef]

B.H. Park, C. Saxer, S.M. Srinivas, J.S. Nelson, J.F. de Boer, �??In vivo burn depth determination by highspeed fiber-based polarization sensitive optical coherence tomography,�?? J. Biomed. Opt. 6, 474-9 (2001).
[CrossRef]

Opt. Express (3)

Opt. Lett. (18)

M.C. Pierce, B.H. Park, B. Cense, J.F. de Boer, �??Simultaneous intensity, birefringence, and flow measurements with high-speed fiber-based optical coherence tomography,�?? Opt. Lett. 27, 1534-6 (2002).

J.E. Roth, J.A. Kozak, S. Yazdanfar, A.M. Rollins, J.A. Izatt, �??Simplified method for polarization-sensitive optical coherence tomography,�?? Opt. Lett. 26, 1069-71 (2001).

V. Westphal, S. Yazdanfar, A.M. Rollins, J.A. Izatt, �??Real-time, high velocity-resolution color Doppler optical coherence tomography,�?? Opt. Lett. 27, 34-6 (2002).

Y. Zhao, Z. Chen, Z. Ding, H. Ren, J.S. Nelson, �??Real-time phase-resolved functional optical coherence tomography by use of optical Hilbert transformation,�?? Opt. Lett. 27, 98-100 (2002).

R. Tripathi, N. Nassif, J.S. Nelson, B.H. Park, J.F. de Boer, �??Spectral shaping for non-Gaussian source spectra in optical coherence tomography,�?? Opt. Lett. 27, 406-8 (2002).

Y. Zhao, Z. Chen, C. Saxer, S. Xiang, J.F. de Boer, J.S. Nelson, �??Phase-resolved optical coherence tomography and optical Doppler tomography for imaging blood flow in human skin with fast scanning speed and high velocity sensitivity,�?? Opt. Lett. 25, 114-6 (2000).

X.J. Wang, T.E. Milner, J.S. Nelson, �??Characterization of fluid-flow velocity by optical Doppler tomography,�?? Opt. Lett. 20, 1337-9 (1995).

J.F. de Boer, T.E. Milner, M.J.C. van Gemert, J.S. Nelson, �??Two dimensional birefringence imaging in biological tissue by polarization-sensitive optical coherence tomography,�?? Opt. Lett. 22, 934-6 (1997).

Z. Chen, T.E. Milner, S. Srinivas, X. Wang, A. Malekafzali, M.J.C. van Gemert, J.S. Nelson, �??Noninvasive imaging of in vivo blood flow velocity using optical Doppler tomography,�?? Opt. Lett. 22, 1119-21 (1997).

J.A. Izatt, M.D. Kulkarni, S. Yazdanfar, J.K. Barton, A.J. Welch, �??In vivo bi-directional color Doppler flow imaging of picoliter blood volumes using optical coherence tomography,�?? Opt. Lett. 22, 1439-41 (1997).

G.J. Tearney, B.E. Bouma, J.G. Fujimoto, �??High-speed phase- and group-delay scanning with a grating based phase control delay line,�?? Opt. Lett. 22, 1811-3 (1997).

M.J. Everett, K. Schoenenberger, B.W. Colston, L.B. Da Silva, �??Birefringence characterization of biological tissue by use of optical coherence tomography,�?? Opt. Lett. 23, 228-30 (1998).

J.M. Schmitt, S.H. Xiang, �??Cross-polarized backscatter in optical coherence tomography of biological tissue,�?? Opt. Lett. 23, 1060-2 (1998).

J.F. de Boer, T.E. Milner, J.S. Nelson, �??Determination of the depth-resolved Stokes parameters of light backscattered from turbid media by use of polarization-sensitive optical coherence tomography,�?? Opt. Lett. 24, 300-2 (1999).

G. Yao, L.V. Wang, �??Two dimensional depth-resolved Mueller matrix characterization of biological tissue by optical coherence tomography,�?? Opt. Lett. 24, 537-9 (1999).

W. Drexler, U. Morgner, F.X. Kärtner, C. Pitris, S.A. Boppart, X.D. Li, E.P. Ippen, J.G. Fujimoto, �??In vivo ultrahigh-resolution optical coherence tomography,�?? Opt. Lett. 24, 1221-3 (1999).

C.E. Saxer, J.F. de Boer, B.H. Park, Y. Zhao, Z. Chen, J.S. Nelson, �??High-speed fiber-based polarizationsensitive optical coherence tomography of in vivo human skin,�?? Opt. Lett. 25, 1355-7 (2000).

Y. Zhao, Z. Chen, C. Saxer, Q. Shen, S. Xiang, J.F. de Boer, J.S. Nelson, �??Doppler standard deviation imaging for clinical monitoring of in vivo human skin blood flow,�?? Opt. Lett. 25, 1358 (2000).

Phys. Med. Biol. (1)

T. Lindmo, D.J. Smithies, Z. Chen, J.S. Nelson, T.E. Milner, �??Accuracy and noise in optical Doppler tomography studied by Monte Carlo simulation,�?? Phys. Med. Biol. 43, 3045-64 (1998).
[CrossRef]

Science (1)

D. Huang, E.A. Swanson, C.P. Lin, J.S. Schuman, W.G. Stinson, W. Chang, M.R. Hee, T. Flotte, K. Gregory, C.A. Puliafito, J.G. Fujimoto, �??Optical coherence tomography,�?? Science 254, 1178-81 (1991).
[CrossRef]

Supplementary Material (2)

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