Abstract

Pronounced separation (750 nm) between two individual fluorescence spots in a novel super-resolution microscopy based on a two-color up-conversion fluorescence depletion technique has been investigated. This microscopy has the potential to achieve a spatial resolution (<300nm) of 1/2 the diffraction limit.

© 2003 Optical Society of America

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  1. P. Davidvits and A.M.D. Egger, “Scanning Laser Microscope,” Nature 48, 831, (1969).
    [Crossref]
  2. T. Wilson and C. J. R. Sheppard, Theory and Practice of Scanning Optical Microscopy, (Academic, London, 1984).
  3. P. Jaquinot and B. Roizen-Dossier, Progress in Optics, III, ed. by E. Wolf, (North-Holland, 1964)
  4. I. Leiserson, S. G. Lipson, and V. Sarafits, “Superresolution in far-field imaing,” Opt. Lett. 25, 209–211 (2000)
    [Crossref]
  5. W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248, 73–76 (1990).
    [Crossref] [PubMed]
  6. S.W. Hell and J. Wichmann, “Breaking the diffraction resolution limit by stimulated emission: stimulate-demission-depletion fluorescence microscopy,” Opt. Lett. 19, 780–782 (1994).
    [Crossref] [PubMed]
  7. T. A. Klar and S. W. Hell, “Subdiffraction limit in far-field fluorescence dip microscopy,” Opt. Lett. 24, 954–956 (1999).
    [Crossref]
  8. Fujii and M. Ito, Advances in Multiphoton Processes and Spectroscopy, Vol. 4, ed. by S. H. Lin (World Scientific, 1988).
  9. T. Ebata, N. Mizuochi, T. Watanabe, and N. Mikami, “OH stretching vibrations of Phenol-(H2O)1 and Phenol-(H2O)3 in the S1 State,” J. Phys. Chem. 100, 546–550 (1996).
    [Crossref]
  10. Y. Iketaki, T. Omatsu, O. Sato, T. Suzuki, and M. Fujii, “Super-resolution fluorescence microscopy by up-conversion-depletion using two color lasers,” in Trends in Optics and Photonics, vol. 39 of OSA Proceedings Series (Optical Society of America, Washington, D.C., 2000), pp.167–168.
  11. T. Watanabe, Y. Iketaki, T. Omatsu, K. Yamamoto, S. Ishiuchi, M. Sakai, and M. Fujii, “Two color far-field super-resolution microscope using a doughnut beam,” J. Chem. Phys. 371, 634–639 (2003).
  12. T. Watanabe, Y. Iketaki, T. Omatsu, K. Yamamoto, S. Ishiuchi, M. Sakai, and M. Fujii, “Far-field super-resolution scanning fluorescence microscope using up-conversion-depletion technique,” in Trends in Optics and Photonics, OSA Proceedings Series (Optical Society of America, Washington, D.C., 2003), in press.
  13. Y. Iketaki, T. Watanabe, S. Ishiuchi, M. Sakai, T. Omatsu, K. Yamamoto, and M. Fujii, “Predicted spatial resolution of super-resolving fluorescence microscopy using two-color fluorescence dip spectroscopy,” Appl. Spectrosc. 57, 1312–1316 (2003).
    [Crossref] [PubMed]
  14. E. H. K. Stelzer, “Beyond the diffraction limit?” Nature,  417, 806–807 (2002).
    [Crossref] [PubMed]
  15. E. Sahar and D. Treves, “Excitation singlet-state absorption in dyes and their effect on dy lasers,” IEEE J. Quntum Electronics 13, 962–967 (1977)
    [Crossref]
  16. Y. Kobayashi, Y. Igasaki, N. Yoshida, N. Fukuchi, H. Toyoda, T. Hara, and M. H. Wu, “Compact high-efficiency electrically-addressable phase-only spatial light modulator,” in Diffractive/Holographic Technologies and Spatial Light Modulators VII, I. Cindrich, S. H. Lee, and R. L. Sutherland, Proc. SPIE3951, 158–165 (2000).
    [Crossref]
  17. D. Ganic, X. Gan, M. Gu, M. Hain, S. Somalingam, S. Stankovic, and T. Tschudi, “Generation of doughnut laser beams by use of a liquid-crystal cell with a conversion efficiency near 100%,” Opt. Lett. 27, 1351–1353 (2002).
    [Crossref]
  18. G. Machavariani, N. Davidson, E. Hasman, S. Blit, A. A. Ishaaya, and A. A. Friesem, “Efficient conversion of a Gaussian beam to a high purity helical beam,” Opt. Commun. 209, 265–271 (2002)
    [Crossref]

2003 (2)

T. Watanabe, Y. Iketaki, T. Omatsu, K. Yamamoto, S. Ishiuchi, M. Sakai, and M. Fujii, “Two color far-field super-resolution microscope using a doughnut beam,” J. Chem. Phys. 371, 634–639 (2003).

Y. Iketaki, T. Watanabe, S. Ishiuchi, M. Sakai, T. Omatsu, K. Yamamoto, and M. Fujii, “Predicted spatial resolution of super-resolving fluorescence microscopy using two-color fluorescence dip spectroscopy,” Appl. Spectrosc. 57, 1312–1316 (2003).
[Crossref] [PubMed]

2002 (3)

E. H. K. Stelzer, “Beyond the diffraction limit?” Nature,  417, 806–807 (2002).
[Crossref] [PubMed]

D. Ganic, X. Gan, M. Gu, M. Hain, S. Somalingam, S. Stankovic, and T. Tschudi, “Generation of doughnut laser beams by use of a liquid-crystal cell with a conversion efficiency near 100%,” Opt. Lett. 27, 1351–1353 (2002).
[Crossref]

G. Machavariani, N. Davidson, E. Hasman, S. Blit, A. A. Ishaaya, and A. A. Friesem, “Efficient conversion of a Gaussian beam to a high purity helical beam,” Opt. Commun. 209, 265–271 (2002)
[Crossref]

2000 (1)

1999 (1)

1996 (1)

T. Ebata, N. Mizuochi, T. Watanabe, and N. Mikami, “OH stretching vibrations of Phenol-(H2O)1 and Phenol-(H2O)3 in the S1 State,” J. Phys. Chem. 100, 546–550 (1996).
[Crossref]

1994 (1)

1990 (1)

W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248, 73–76 (1990).
[Crossref] [PubMed]

1977 (1)

E. Sahar and D. Treves, “Excitation singlet-state absorption in dyes and their effect on dy lasers,” IEEE J. Quntum Electronics 13, 962–967 (1977)
[Crossref]

1969 (1)

P. Davidvits and A.M.D. Egger, “Scanning Laser Microscope,” Nature 48, 831, (1969).
[Crossref]

Blit, S.

G. Machavariani, N. Davidson, E. Hasman, S. Blit, A. A. Ishaaya, and A. A. Friesem, “Efficient conversion of a Gaussian beam to a high purity helical beam,” Opt. Commun. 209, 265–271 (2002)
[Crossref]

Cindrich, I.

Y. Kobayashi, Y. Igasaki, N. Yoshida, N. Fukuchi, H. Toyoda, T. Hara, and M. H. Wu, “Compact high-efficiency electrically-addressable phase-only spatial light modulator,” in Diffractive/Holographic Technologies and Spatial Light Modulators VII, I. Cindrich, S. H. Lee, and R. L. Sutherland, Proc. SPIE3951, 158–165 (2000).
[Crossref]

Davidson, N.

G. Machavariani, N. Davidson, E. Hasman, S. Blit, A. A. Ishaaya, and A. A. Friesem, “Efficient conversion of a Gaussian beam to a high purity helical beam,” Opt. Commun. 209, 265–271 (2002)
[Crossref]

Davidvits, P.

P. Davidvits and A.M.D. Egger, “Scanning Laser Microscope,” Nature 48, 831, (1969).
[Crossref]

Denk, W.

W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248, 73–76 (1990).
[Crossref] [PubMed]

Ebata, T.

T. Ebata, N. Mizuochi, T. Watanabe, and N. Mikami, “OH stretching vibrations of Phenol-(H2O)1 and Phenol-(H2O)3 in the S1 State,” J. Phys. Chem. 100, 546–550 (1996).
[Crossref]

Egger, A.M.D.

P. Davidvits and A.M.D. Egger, “Scanning Laser Microscope,” Nature 48, 831, (1969).
[Crossref]

Friesem, A. A.

G. Machavariani, N. Davidson, E. Hasman, S. Blit, A. A. Ishaaya, and A. A. Friesem, “Efficient conversion of a Gaussian beam to a high purity helical beam,” Opt. Commun. 209, 265–271 (2002)
[Crossref]

Fujii,

Fujii and M. Ito, Advances in Multiphoton Processes and Spectroscopy, Vol. 4, ed. by S. H. Lin (World Scientific, 1988).

Fujii, M.

T. Watanabe, Y. Iketaki, T. Omatsu, K. Yamamoto, S. Ishiuchi, M. Sakai, and M. Fujii, “Two color far-field super-resolution microscope using a doughnut beam,” J. Chem. Phys. 371, 634–639 (2003).

Y. Iketaki, T. Watanabe, S. Ishiuchi, M. Sakai, T. Omatsu, K. Yamamoto, and M. Fujii, “Predicted spatial resolution of super-resolving fluorescence microscopy using two-color fluorescence dip spectroscopy,” Appl. Spectrosc. 57, 1312–1316 (2003).
[Crossref] [PubMed]

T. Watanabe, Y. Iketaki, T. Omatsu, K. Yamamoto, S. Ishiuchi, M. Sakai, and M. Fujii, “Far-field super-resolution scanning fluorescence microscope using up-conversion-depletion technique,” in Trends in Optics and Photonics, OSA Proceedings Series (Optical Society of America, Washington, D.C., 2003), in press.

Y. Iketaki, T. Omatsu, O. Sato, T. Suzuki, and M. Fujii, “Super-resolution fluorescence microscopy by up-conversion-depletion using two color lasers,” in Trends in Optics and Photonics, vol. 39 of OSA Proceedings Series (Optical Society of America, Washington, D.C., 2000), pp.167–168.

Fukuchi, N.

Y. Kobayashi, Y. Igasaki, N. Yoshida, N. Fukuchi, H. Toyoda, T. Hara, and M. H. Wu, “Compact high-efficiency electrically-addressable phase-only spatial light modulator,” in Diffractive/Holographic Technologies and Spatial Light Modulators VII, I. Cindrich, S. H. Lee, and R. L. Sutherland, Proc. SPIE3951, 158–165 (2000).
[Crossref]

Gan, X.

Ganic, D.

Gu, M.

Hain, M.

Hara, T.

Y. Kobayashi, Y. Igasaki, N. Yoshida, N. Fukuchi, H. Toyoda, T. Hara, and M. H. Wu, “Compact high-efficiency electrically-addressable phase-only spatial light modulator,” in Diffractive/Holographic Technologies and Spatial Light Modulators VII, I. Cindrich, S. H. Lee, and R. L. Sutherland, Proc. SPIE3951, 158–165 (2000).
[Crossref]

Hasman, E.

G. Machavariani, N. Davidson, E. Hasman, S. Blit, A. A. Ishaaya, and A. A. Friesem, “Efficient conversion of a Gaussian beam to a high purity helical beam,” Opt. Commun. 209, 265–271 (2002)
[Crossref]

Hell, S. W.

Hell, S.W.

Igasaki, Y.

Y. Kobayashi, Y. Igasaki, N. Yoshida, N. Fukuchi, H. Toyoda, T. Hara, and M. H. Wu, “Compact high-efficiency electrically-addressable phase-only spatial light modulator,” in Diffractive/Holographic Technologies and Spatial Light Modulators VII, I. Cindrich, S. H. Lee, and R. L. Sutherland, Proc. SPIE3951, 158–165 (2000).
[Crossref]

Iketaki, Y.

Y. Iketaki, T. Watanabe, S. Ishiuchi, M. Sakai, T. Omatsu, K. Yamamoto, and M. Fujii, “Predicted spatial resolution of super-resolving fluorescence microscopy using two-color fluorescence dip spectroscopy,” Appl. Spectrosc. 57, 1312–1316 (2003).
[Crossref] [PubMed]

T. Watanabe, Y. Iketaki, T. Omatsu, K. Yamamoto, S. Ishiuchi, M. Sakai, and M. Fujii, “Two color far-field super-resolution microscope using a doughnut beam,” J. Chem. Phys. 371, 634–639 (2003).

T. Watanabe, Y. Iketaki, T. Omatsu, K. Yamamoto, S. Ishiuchi, M. Sakai, and M. Fujii, “Far-field super-resolution scanning fluorescence microscope using up-conversion-depletion technique,” in Trends in Optics and Photonics, OSA Proceedings Series (Optical Society of America, Washington, D.C., 2003), in press.

Y. Iketaki, T. Omatsu, O. Sato, T. Suzuki, and M. Fujii, “Super-resolution fluorescence microscopy by up-conversion-depletion using two color lasers,” in Trends in Optics and Photonics, vol. 39 of OSA Proceedings Series (Optical Society of America, Washington, D.C., 2000), pp.167–168.

Ishaaya, A. A.

G. Machavariani, N. Davidson, E. Hasman, S. Blit, A. A. Ishaaya, and A. A. Friesem, “Efficient conversion of a Gaussian beam to a high purity helical beam,” Opt. Commun. 209, 265–271 (2002)
[Crossref]

Ishiuchi, S.

Y. Iketaki, T. Watanabe, S. Ishiuchi, M. Sakai, T. Omatsu, K. Yamamoto, and M. Fujii, “Predicted spatial resolution of super-resolving fluorescence microscopy using two-color fluorescence dip spectroscopy,” Appl. Spectrosc. 57, 1312–1316 (2003).
[Crossref] [PubMed]

T. Watanabe, Y. Iketaki, T. Omatsu, K. Yamamoto, S. Ishiuchi, M. Sakai, and M. Fujii, “Two color far-field super-resolution microscope using a doughnut beam,” J. Chem. Phys. 371, 634–639 (2003).

T. Watanabe, Y. Iketaki, T. Omatsu, K. Yamamoto, S. Ishiuchi, M. Sakai, and M. Fujii, “Far-field super-resolution scanning fluorescence microscope using up-conversion-depletion technique,” in Trends in Optics and Photonics, OSA Proceedings Series (Optical Society of America, Washington, D.C., 2003), in press.

Ito, M.

Fujii and M. Ito, Advances in Multiphoton Processes and Spectroscopy, Vol. 4, ed. by S. H. Lin (World Scientific, 1988).

Jaquinot, P.

P. Jaquinot and B. Roizen-Dossier, Progress in Optics, III, ed. by E. Wolf, (North-Holland, 1964)

Klar, T. A.

Kobayashi, Y.

Y. Kobayashi, Y. Igasaki, N. Yoshida, N. Fukuchi, H. Toyoda, T. Hara, and M. H. Wu, “Compact high-efficiency electrically-addressable phase-only spatial light modulator,” in Diffractive/Holographic Technologies and Spatial Light Modulators VII, I. Cindrich, S. H. Lee, and R. L. Sutherland, Proc. SPIE3951, 158–165 (2000).
[Crossref]

Lee, S. H.

Y. Kobayashi, Y. Igasaki, N. Yoshida, N. Fukuchi, H. Toyoda, T. Hara, and M. H. Wu, “Compact high-efficiency electrically-addressable phase-only spatial light modulator,” in Diffractive/Holographic Technologies and Spatial Light Modulators VII, I. Cindrich, S. H. Lee, and R. L. Sutherland, Proc. SPIE3951, 158–165 (2000).
[Crossref]

Leiserson, I.

Lipson, S. G.

Machavariani, G.

G. Machavariani, N. Davidson, E. Hasman, S. Blit, A. A. Ishaaya, and A. A. Friesem, “Efficient conversion of a Gaussian beam to a high purity helical beam,” Opt. Commun. 209, 265–271 (2002)
[Crossref]

Mikami, N.

T. Ebata, N. Mizuochi, T. Watanabe, and N. Mikami, “OH stretching vibrations of Phenol-(H2O)1 and Phenol-(H2O)3 in the S1 State,” J. Phys. Chem. 100, 546–550 (1996).
[Crossref]

Mizuochi, N.

T. Ebata, N. Mizuochi, T. Watanabe, and N. Mikami, “OH stretching vibrations of Phenol-(H2O)1 and Phenol-(H2O)3 in the S1 State,” J. Phys. Chem. 100, 546–550 (1996).
[Crossref]

Omatsu, T.

T. Watanabe, Y. Iketaki, T. Omatsu, K. Yamamoto, S. Ishiuchi, M. Sakai, and M. Fujii, “Two color far-field super-resolution microscope using a doughnut beam,” J. Chem. Phys. 371, 634–639 (2003).

Y. Iketaki, T. Watanabe, S. Ishiuchi, M. Sakai, T. Omatsu, K. Yamamoto, and M. Fujii, “Predicted spatial resolution of super-resolving fluorescence microscopy using two-color fluorescence dip spectroscopy,” Appl. Spectrosc. 57, 1312–1316 (2003).
[Crossref] [PubMed]

T. Watanabe, Y. Iketaki, T. Omatsu, K. Yamamoto, S. Ishiuchi, M. Sakai, and M. Fujii, “Far-field super-resolution scanning fluorescence microscope using up-conversion-depletion technique,” in Trends in Optics and Photonics, OSA Proceedings Series (Optical Society of America, Washington, D.C., 2003), in press.

Y. Iketaki, T. Omatsu, O. Sato, T. Suzuki, and M. Fujii, “Super-resolution fluorescence microscopy by up-conversion-depletion using two color lasers,” in Trends in Optics and Photonics, vol. 39 of OSA Proceedings Series (Optical Society of America, Washington, D.C., 2000), pp.167–168.

Roizen-Dossier, B.

P. Jaquinot and B. Roizen-Dossier, Progress in Optics, III, ed. by E. Wolf, (North-Holland, 1964)

Sahar, E.

E. Sahar and D. Treves, “Excitation singlet-state absorption in dyes and their effect on dy lasers,” IEEE J. Quntum Electronics 13, 962–967 (1977)
[Crossref]

Sakai, M.

Y. Iketaki, T. Watanabe, S. Ishiuchi, M. Sakai, T. Omatsu, K. Yamamoto, and M. Fujii, “Predicted spatial resolution of super-resolving fluorescence microscopy using two-color fluorescence dip spectroscopy,” Appl. Spectrosc. 57, 1312–1316 (2003).
[Crossref] [PubMed]

T. Watanabe, Y. Iketaki, T. Omatsu, K. Yamamoto, S. Ishiuchi, M. Sakai, and M. Fujii, “Two color far-field super-resolution microscope using a doughnut beam,” J. Chem. Phys. 371, 634–639 (2003).

T. Watanabe, Y. Iketaki, T. Omatsu, K. Yamamoto, S. Ishiuchi, M. Sakai, and M. Fujii, “Far-field super-resolution scanning fluorescence microscope using up-conversion-depletion technique,” in Trends in Optics and Photonics, OSA Proceedings Series (Optical Society of America, Washington, D.C., 2003), in press.

Sarafits, V.

Sato, O.

Y. Iketaki, T. Omatsu, O. Sato, T. Suzuki, and M. Fujii, “Super-resolution fluorescence microscopy by up-conversion-depletion using two color lasers,” in Trends in Optics and Photonics, vol. 39 of OSA Proceedings Series (Optical Society of America, Washington, D.C., 2000), pp.167–168.

Sheppard, C. J. R.

T. Wilson and C. J. R. Sheppard, Theory and Practice of Scanning Optical Microscopy, (Academic, London, 1984).

Somalingam, S.

Stankovic, S.

Stelzer, E. H. K.

E. H. K. Stelzer, “Beyond the diffraction limit?” Nature,  417, 806–807 (2002).
[Crossref] [PubMed]

Strickler, J. H.

W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248, 73–76 (1990).
[Crossref] [PubMed]

Sutherland, R. L.

Y. Kobayashi, Y. Igasaki, N. Yoshida, N. Fukuchi, H. Toyoda, T. Hara, and M. H. Wu, “Compact high-efficiency electrically-addressable phase-only spatial light modulator,” in Diffractive/Holographic Technologies and Spatial Light Modulators VII, I. Cindrich, S. H. Lee, and R. L. Sutherland, Proc. SPIE3951, 158–165 (2000).
[Crossref]

Suzuki, T.

Y. Iketaki, T. Omatsu, O. Sato, T. Suzuki, and M. Fujii, “Super-resolution fluorescence microscopy by up-conversion-depletion using two color lasers,” in Trends in Optics and Photonics, vol. 39 of OSA Proceedings Series (Optical Society of America, Washington, D.C., 2000), pp.167–168.

Toyoda, H.

Y. Kobayashi, Y. Igasaki, N. Yoshida, N. Fukuchi, H. Toyoda, T. Hara, and M. H. Wu, “Compact high-efficiency electrically-addressable phase-only spatial light modulator,” in Diffractive/Holographic Technologies and Spatial Light Modulators VII, I. Cindrich, S. H. Lee, and R. L. Sutherland, Proc. SPIE3951, 158–165 (2000).
[Crossref]

Treves, D.

E. Sahar and D. Treves, “Excitation singlet-state absorption in dyes and their effect on dy lasers,” IEEE J. Quntum Electronics 13, 962–967 (1977)
[Crossref]

Tschudi, T.

Watanabe, T.

Y. Iketaki, T. Watanabe, S. Ishiuchi, M. Sakai, T. Omatsu, K. Yamamoto, and M. Fujii, “Predicted spatial resolution of super-resolving fluorescence microscopy using two-color fluorescence dip spectroscopy,” Appl. Spectrosc. 57, 1312–1316 (2003).
[Crossref] [PubMed]

T. Watanabe, Y. Iketaki, T. Omatsu, K. Yamamoto, S. Ishiuchi, M. Sakai, and M. Fujii, “Two color far-field super-resolution microscope using a doughnut beam,” J. Chem. Phys. 371, 634–639 (2003).

T. Ebata, N. Mizuochi, T. Watanabe, and N. Mikami, “OH stretching vibrations of Phenol-(H2O)1 and Phenol-(H2O)3 in the S1 State,” J. Phys. Chem. 100, 546–550 (1996).
[Crossref]

T. Watanabe, Y. Iketaki, T. Omatsu, K. Yamamoto, S. Ishiuchi, M. Sakai, and M. Fujii, “Far-field super-resolution scanning fluorescence microscope using up-conversion-depletion technique,” in Trends in Optics and Photonics, OSA Proceedings Series (Optical Society of America, Washington, D.C., 2003), in press.

Webb, W. W.

W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248, 73–76 (1990).
[Crossref] [PubMed]

Wichmann, J.

Wilson, T.

T. Wilson and C. J. R. Sheppard, Theory and Practice of Scanning Optical Microscopy, (Academic, London, 1984).

Wu, M. H.

Y. Kobayashi, Y. Igasaki, N. Yoshida, N. Fukuchi, H. Toyoda, T. Hara, and M. H. Wu, “Compact high-efficiency electrically-addressable phase-only spatial light modulator,” in Diffractive/Holographic Technologies and Spatial Light Modulators VII, I. Cindrich, S. H. Lee, and R. L. Sutherland, Proc. SPIE3951, 158–165 (2000).
[Crossref]

Yamamoto, K.

Y. Iketaki, T. Watanabe, S. Ishiuchi, M. Sakai, T. Omatsu, K. Yamamoto, and M. Fujii, “Predicted spatial resolution of super-resolving fluorescence microscopy using two-color fluorescence dip spectroscopy,” Appl. Spectrosc. 57, 1312–1316 (2003).
[Crossref] [PubMed]

T. Watanabe, Y. Iketaki, T. Omatsu, K. Yamamoto, S. Ishiuchi, M. Sakai, and M. Fujii, “Two color far-field super-resolution microscope using a doughnut beam,” J. Chem. Phys. 371, 634–639 (2003).

T. Watanabe, Y. Iketaki, T. Omatsu, K. Yamamoto, S. Ishiuchi, M. Sakai, and M. Fujii, “Far-field super-resolution scanning fluorescence microscope using up-conversion-depletion technique,” in Trends in Optics and Photonics, OSA Proceedings Series (Optical Society of America, Washington, D.C., 2003), in press.

Yoshida, N.

Y. Kobayashi, Y. Igasaki, N. Yoshida, N. Fukuchi, H. Toyoda, T. Hara, and M. H. Wu, “Compact high-efficiency electrically-addressable phase-only spatial light modulator,” in Diffractive/Holographic Technologies and Spatial Light Modulators VII, I. Cindrich, S. H. Lee, and R. L. Sutherland, Proc. SPIE3951, 158–165 (2000).
[Crossref]

Appl. Spectrosc. (1)

IEEE J. Quntum Electronics (1)

E. Sahar and D. Treves, “Excitation singlet-state absorption in dyes and their effect on dy lasers,” IEEE J. Quntum Electronics 13, 962–967 (1977)
[Crossref]

J. Chem. Phys. (1)

T. Watanabe, Y. Iketaki, T. Omatsu, K. Yamamoto, S. Ishiuchi, M. Sakai, and M. Fujii, “Two color far-field super-resolution microscope using a doughnut beam,” J. Chem. Phys. 371, 634–639 (2003).

J. Phys. Chem. (1)

T. Ebata, N. Mizuochi, T. Watanabe, and N. Mikami, “OH stretching vibrations of Phenol-(H2O)1 and Phenol-(H2O)3 in the S1 State,” J. Phys. Chem. 100, 546–550 (1996).
[Crossref]

Nature (2)

P. Davidvits and A.M.D. Egger, “Scanning Laser Microscope,” Nature 48, 831, (1969).
[Crossref]

E. H. K. Stelzer, “Beyond the diffraction limit?” Nature,  417, 806–807 (2002).
[Crossref] [PubMed]

Opt. Commun. (1)

G. Machavariani, N. Davidson, E. Hasman, S. Blit, A. A. Ishaaya, and A. A. Friesem, “Efficient conversion of a Gaussian beam to a high purity helical beam,” Opt. Commun. 209, 265–271 (2002)
[Crossref]

Opt. Lett. (4)

Science (1)

W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248, 73–76 (1990).
[Crossref] [PubMed]

Other (6)

T. Wilson and C. J. R. Sheppard, Theory and Practice of Scanning Optical Microscopy, (Academic, London, 1984).

P. Jaquinot and B. Roizen-Dossier, Progress in Optics, III, ed. by E. Wolf, (North-Holland, 1964)

Fujii and M. Ito, Advances in Multiphoton Processes and Spectroscopy, Vol. 4, ed. by S. H. Lin (World Scientific, 1988).

Y. Iketaki, T. Omatsu, O. Sato, T. Suzuki, and M. Fujii, “Super-resolution fluorescence microscopy by up-conversion-depletion using two color lasers,” in Trends in Optics and Photonics, vol. 39 of OSA Proceedings Series (Optical Society of America, Washington, D.C., 2000), pp.167–168.

Y. Kobayashi, Y. Igasaki, N. Yoshida, N. Fukuchi, H. Toyoda, T. Hara, and M. H. Wu, “Compact high-efficiency electrically-addressable phase-only spatial light modulator,” in Diffractive/Holographic Technologies and Spatial Light Modulators VII, I. Cindrich, S. H. Lee, and R. L. Sutherland, Proc. SPIE3951, 158–165 (2000).
[Crossref]

T. Watanabe, Y. Iketaki, T. Omatsu, K. Yamamoto, S. Ishiuchi, M. Sakai, and M. Fujii, “Far-field super-resolution scanning fluorescence microscope using up-conversion-depletion technique,” in Trends in Optics and Photonics, OSA Proceedings Series (Optical Society of America, Washington, D.C., 2003), in press.

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Figures (4)

Fig. 1.
Fig. 1.

(a) Excitation scheme of a two-color up-conversion fluorescence depletion technique. (b) Scheme for super-resolution microscopy based on a two-color up-conversion fluorescence depletion technique. The pump and “doughnut-shape” erase light are coaxially introduced to the sample.

Fig. 2.
Fig. 2.

Schematic diagram of the experimental setup for two-color super-resolution microscopy using a doughnut beam. PAL-SLM corresponds to a spatial light modulator.

Fig. 3.
Fig. 3.

Experimental scanning image of the ϕ175 nm micro-sphere obtained by (a) pump and “doughnut” erase beams, and (b) pump light only. The cross section is also shown below the images (c and d).

Fig. 4.
Fig. 4.

Experimental scanning image of micro-spheres obtained by (a) pump and “doughnut” erase beams, and (b) pump light only. The cross section profile along the red line is shown below the image (c and d).

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