Abstract

An experimental standard optical coherence tomography (OCT) setup that can be easily modified for cross-polarization OCT (CP OCT) operation has been developed to perform differential diagnosis of pathological tissues. The complementary use of CP OCT, a technique that provides a map of cross-polarization backscattering properties of an object being studied by means of low-coherence interferometry, and standard OCT imaging improves the specificity of diagnostics of pathological changes occurring in tissues. It is shown that healthy, neoplastic and scar tissues of the esophagus have different cross-polarization backscattering properties. A comparative analysis of CP OCT, OCT and histological images from one and the same tissue area has been made. A close correlation between the location of collagen fibers in biological tissue and signal intensity in CP OCT images is found.

© 2002 Optical Society of America

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References

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Am. J. Gastroenter. (1)

G. Zuccaro, N.D. Gladkova, J. Vargo, F.I. Feldchtein, E.V. Zagaynova, D. Conwell, G.W. Falk, J.R. Goldblum, J. Dumot, J. Ponsky, G.V. Gelikonov, B. Davros, E. Donchenko, J.E. Richter, ???Optical coherence tomography of the esophagus and proximal stomach in health and disease,??? Am. J. Gastroenter. 96, 2633-2639, (2001).
[CrossRef] [PubMed]

Appl. Opt. (1)

Caries Research (1)

A. Baumgartner, S. Dichtl, C.K. Hitzenberger, H. Sattmann, B. Robl, A. Moritz, Z.F. Fercher, W. Sperr, ???Polarization-sensitive optical coherence tomography of dental structures,??? Caries Research 34, 59-69, (2000).
[CrossRef]

IEEE J. Sel. Top. Quantum Electron. (3)

J.M. Schmitt, ???Optical Coherence Tomography (OCT): A Review,??? IEEE J. Sel. Top. Quantum Electron. 5, 1205-1215, (1999).
[CrossRef]

M.E. Brezinski, J.G. Fujimoto, ???Optical Coherence Tomography: High-Resolution Imaging in Nontransparent Tissue,??? IEEE J. Sel. Top. Quantum Electron. 5, 1185-1192, (1999).
[CrossRef]

J.F. de Boer, S.M. Srinivas, B.H. Park, T.H. Pham, Z.P. Chen, T.E. Milner, J.S. Nelson, ???Polarization effects in optical coherence tomography of various biological tissues,??? IEEE J. Sel. Top. Quantum Electron. 5, 1200-1204, (1999).
[CrossRef]

J. Gastroenterol. (1)

C. Pitris, C. Jesser, S.A. Boppart, D. Stemper, M.E. Brezinski, J.G. Fujimoto, ???Feasibility of optical coherence tomography for high-resolution imaging of human gastrointestinal tract malignancies,??? J. Gastroenterol. 35, 87-92, (2000).
[CrossRef] [PubMed]

J. Rheumatol. (1)

W. Drexler, D. Stamper, C. Jesser, X.D. Li, C. Pitris, K. Saunders, S. Martin, M.B. Lodge, J.G. Fujimoto, M.E. Brezinski, ???Correlation of collagen organization with polarization sensitive imaging of in vitro cartilage: Implications for osteoarthritis,??? J. Rheumatol. 28, 1311-1318, (2001).
[PubMed]

J. Surg. Oncology (1)

A.V. Shakhov, A.B. Terentjeva, V.A. Kamensky, L.B. Snopova, V.M. Gelikonov, F.I. Feldchtein, A.M. Sergeev, ???Optical Coherence Tomography Monitoring for Laser Surgery of Laryngeal Carcinoma,??? J. Surg. Oncology 77, 253-259, (2001).
[CrossRef]

J. Urology (1)

E.V. Zagaynova, O.S. Streltsova, N.D. Gladkova, L.B. Snopova, G.V. Gelikonov, F.I. Feldchtein, A.N. Morozov, ???In vivo optical coherence tomography feasibility for bladder disease,??? J. Urology 167, 1492-1496, (2002).
[CrossRef]

JETP Lett. (1)

V.M. Gelikonov, G.V. Gelikonov, R.V. Kuranov, K.I. Pravdenko, A.M. Sergeev, F.I. Feldchtein, Ya.I. Khanin, D.V. Shabanov, N.D. Gladkova, N.K. Nikulin, G.A. Petrova, and V.V. Pochinko, "Coherent optical tomography of microscopic inhomogeneties in biological tissues," JETP Lett. 61, 158-162, (1995).

Laser Phys. (1)

N.M. Shakhova, V.M. Gelikonov, V.A. Kamensky, R.V. Kuranov, and I.V. Turchin, ???Clinical aspects of the endoscopic optical coherence tomography and the ways for improving its diagnostic value,??? Laser Phys. 12, 617-626, (2002).

Lasers Sur. Med. (2)

S.L. Jacques, J.R. Roman, K. Lee, ???Imaging superficial tissues with polarized light,??? Lasers Sur. Med. 26, 119-129, (2000).
[CrossRef]

D.J. Maitland, J.T. Jr Walsh, ???Quantitative measurements of linear birefringence during heating of native collagen,??? Lasers Sur. Med. 20, 310-318, (1997).
[CrossRef]

Opt. Express (2)

Opt. Lett. (1)

OSA Biomed. Top. Meeting (1)

R.V. Kuranov, V.M. Gelikonov, A.V. Shakhov, A.B. Terentyeva, I.V. Turchin and V.A. Kamensky, ???Mapping depolarization properties of biotissues for increasing specificity of OCT,??? in OSA Biomed. Top. Meeting, OSA Tech. Digest, (Optical Society of America, Washington DC, 2002), pp.275-277.

Science (1)

D. Huang, J. Wang, C.P. Lin, J. S. Shuman, W.G. Stinson, W. Chang, M.R. Hee, T. Flotte, K. Gregory, C.A. Puliafito, and J.G. Fujimoto, "Optical coherence tomography," Science 254, 1178-1181, (1991).
[CrossRef] [PubMed]

Skin Research and Technology (1)

N.D. Gladkova, G.A. Petrova, N.K. Nikulin, S.G. Radenska-Lopovok, L.B. Snopova,. V.A. Nasonova, G.V. Gelikonov, V.M Gelikonov, R.V. Kuranov, A.M. Sergeev, F.I. Feldchtein,???In vivo optical coherence tomography imaging of human skin: norm and pathology,??? Skin Research and Technology 6, 6-16, (2000).
[CrossRef]

Sov. Tech. Phys. Lett. (1)

V.M. Gelikonov, D.D. Gusovskii, V.I. Leonov, M.A. Novikov, ???Birefringence compensation in single-mode optical fibers,??? Sov. Tech. Phys. Lett. 13, 322-323, (1987).

Other (1)

R.D. Lillie, Histopathologic technic and practical histochemistry (McGraw-Hill book company, New York-Toronto-Sydney-London, 1965), Chap. 15, or <a href="http://www.ebsciences.com/staining/van_gies.htm">http://www.ebsciences.com/staining/van_gies.htm</a>.

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Figures (5)

Fig. 1.
Fig. 1.

Standard OCT images printed in logarithmic scale of different diseases a) laryngitis (chronic inflammation of larynx) b) laryngeal cancer c) laryngeal papilloma. White bar corresponds to 1 mm.

Fig.2
Fig.2

The experimental setup for the cross-polarization OCT. CS – crossectional scanner, O – investigated object, PS – longitudinal piezo-scanner, L – lenses, PD – photodiode, SA – selective amplifier, LA – logarithmic amplifier, AD – amplitude detector, ADC – analog to digital converter, PC – personal computer. Bold line corresponds to single-mode fiber, thin line - to polarization maintaining fiber.

Fig. 3.
Fig. 3.

a) Standard OCT image, b) CP OCT image c) H&E histology d), e) Van Gieson histology of healthy esophagus. White bar corresponds to 1 mm.

Fig. 4.
Fig. 4.

a) Standard OCT image, b) CP OCT image c) H&E histology d),e) Van Gieson histology of cancerous esophagus. White bar corresponds to 1 mm where not specially marked.

Fig. 5.
Fig. 5.

a) Standard OCT image, b) CP OCT image c) H&E histology d),e) Van Gieson histology of scar esophagus. White bar corresponds to 1 mm where not specially marked.

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