Abstract

We demonstrate a technique for obtaining fully dynamic multiple-beam optical tweezers using the generalized phase contrast (GPC) method and a phase-only spatial light modulator (SLM). The GPC method facilitates the direct transformation of an input phase pattern to an array of high-intensity beams, which can function as efficient multiple optical traps. This straightforward process enables an adjustable number of traps and real-time control of the position, size, shape and intensity of each individual tweezer-beam in arbitrary arrays by encoding the appropriate phase pattern on the SLM. Experimental results show trapping and dynamic manipulation of multiple micro-spheres in a liquid solution.

© 2002 Optical Society of America

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    [CrossRef] [PubMed]
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    [CrossRef] [PubMed]
  3. R. E. Holmlin, M. Schiavoni, C. Y. Chen, S. P. Smith, M. G. Prentiss, and G. M. Whitesides, “Light-driven microfabrication: Assembly of multi-component, three-dimensional structures by using optical tweezers,” Angew. Chem. Int. Ed. Engl. 39, 3503 (2000).
    [CrossRef] [PubMed]
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    [CrossRef]
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    [CrossRef] [PubMed]
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    [CrossRef]
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    [CrossRef] [PubMed]
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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
  14. Y. Kobayashi, et al, “Compact High-efficiency Electrically-addressable Phase-only Spatial Light Modulator,” Proc. of SPIE 3951, 158 (2000).
    [CrossRef]
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    [CrossRef]
  16. E. Higurashi, R. Sawada, and T. Ito, “Optically induced angular alignment of trapped birefringent micro-objects by linear polarization,” Appl. Phys. Lett. 73, 3034 (1998)
    [CrossRef]
  17. S. Grover, A. Skirtach, R. Gauther, and C. Grover, “Automated single-cell sorting system based on optical trapping, ” J. Biomed. Opt. 6, 14 (2001).
    [CrossRef] [PubMed]

2002 (2)

R. L. Eriksen, P.C. Mogensen, and J. Glückstad, “Multiple beam optical tweezers generated by the generalized phase contrast method,” Opt. Lett. 27, 267 (2002).
[CrossRef]

M. P. MacDonald, L. Paterson, K. Volke-Sepulveda, J. Arlt, W. Sibbett, and K. Dholakia, “Creation and manipulation of three-dimensional optically trapped structures,” Science 296, 1101 (2002).
[CrossRef] [PubMed]

2001 (4)

Y. Ogura, K. Kagawa, and J. Tanida, “Optical Manipulation of Microscopic Objects by means of Vertical-Cavity Surface-Emitting Laser Array Sources,” Appl. Opt. 40, 5430 (2001).
[CrossRef]

J. Glückstad and P. C. Mogensen, “Optimal phase contrast in common-path interferometry,” Appl. Opt. 40, 268 (2001).
[CrossRef]

E. R. Dufresne, G. C. Spalding, M. T. Dearing, S. A. Sheets, and D. G. Grier, “Computer-generated holographic optical tweezer arrays,” Rev. Sci. Instrum. 72, 1810 (2001).
[CrossRef]

S. Grover, A. Skirtach, R. Gauther, and C. Grover, “Automated single-cell sorting system based on optical trapping, ” J. Biomed. Opt. 6, 14 (2001).
[CrossRef] [PubMed]

2000 (3)

J. Liesener, M. Reicherter, T. Haist, and H. J. Tiziani, “Multi-functional optical tweezers using computer-generated holograms,” Opt. Commun. 185, 77 (2000).
[CrossRef]

Y. Kobayashi, et al, “Compact High-efficiency Electrically-addressable Phase-only Spatial Light Modulator,” Proc. of SPIE 3951, 158 (2000).
[CrossRef]

R. E. Holmlin, M. Schiavoni, C. Y. Chen, S. P. Smith, M. G. Prentiss, and G. M. Whitesides, “Light-driven microfabrication: Assembly of multi-component, three-dimensional structures by using optical tweezers,” Angew. Chem. Int. Ed. Engl. 39, 3503 (2000).
[CrossRef] [PubMed]

1998 (3)

M. Friese, T. Nieminen, N. Heckenberg, and H. Rubinsztein-Dunlop, “Optical alignment and spinning of laser-trapped microscopic particles,” Nature 394, 348 (1998).
[CrossRef]

E. Higurashi, R. Sawada, and T. Ito, “Optically induced angular alignment of trapped birefringent micro-objects by linear polarization,” Appl. Phys. Lett. 73, 3034 (1998)
[CrossRef]

E. R. Dufresne and D. G. Grier, “Optical tweezer arrays and optical substrates created with diffractive optics,” Rev. Sci. Instrum. 69, 1974 (1998).
[CrossRef]

1997 (1)

1994 (1)

K. Svoboda and S. M Block, “Biological applications of optical forces, ”Annu. Rev. Biophys. Biomol. Struct. 23, 247 (1994).
[CrossRef] [PubMed]

1991 (1)

1986 (1)

Arlt, J.

M. P. MacDonald, L. Paterson, K. Volke-Sepulveda, J. Arlt, W. Sibbett, and K. Dholakia, “Creation and manipulation of three-dimensional optically trapped structures,” Science 296, 1101 (2002).
[CrossRef] [PubMed]

Ashkin, A.

Axner, O.

Bjorkholm, J. E.

Block, S. M

K. Svoboda and S. M Block, “Biological applications of optical forces, ”Annu. Rev. Biophys. Biomol. Struct. 23, 247 (1994).
[CrossRef] [PubMed]

Chen, C. Y.

R. E. Holmlin, M. Schiavoni, C. Y. Chen, S. P. Smith, M. G. Prentiss, and G. M. Whitesides, “Light-driven microfabrication: Assembly of multi-component, three-dimensional structures by using optical tweezers,” Angew. Chem. Int. Ed. Engl. 39, 3503 (2000).
[CrossRef] [PubMed]

Chu, S.

Dearing, M. T.

E. R. Dufresne, G. C. Spalding, M. T. Dearing, S. A. Sheets, and D. G. Grier, “Computer-generated holographic optical tweezer arrays,” Rev. Sci. Instrum. 72, 1810 (2001).
[CrossRef]

Dholakia, K.

M. P. MacDonald, L. Paterson, K. Volke-Sepulveda, J. Arlt, W. Sibbett, and K. Dholakia, “Creation and manipulation of three-dimensional optically trapped structures,” Science 296, 1101 (2002).
[CrossRef] [PubMed]

Dufresne, E. R.

E. R. Dufresne, G. C. Spalding, M. T. Dearing, S. A. Sheets, and D. G. Grier, “Computer-generated holographic optical tweezer arrays,” Rev. Sci. Instrum. 72, 1810 (2001).
[CrossRef]

E. R. Dufresne and D. G. Grier, “Optical tweezer arrays and optical substrates created with diffractive optics,” Rev. Sci. Instrum. 69, 1974 (1998).
[CrossRef]

Dziedic, J. M.

Eriksen, R. L.

Fällman, E.

Friese, M.

M. Friese, T. Nieminen, N. Heckenberg, and H. Rubinsztein-Dunlop, “Optical alignment and spinning of laser-trapped microscopic particles,” Nature 394, 348 (1998).
[CrossRef]

Gauther, R.

S. Grover, A. Skirtach, R. Gauther, and C. Grover, “Automated single-cell sorting system based on optical trapping, ” J. Biomed. Opt. 6, 14 (2001).
[CrossRef] [PubMed]

Glückstad, J.

Grier, D. G.

E. R. Dufresne, G. C. Spalding, M. T. Dearing, S. A. Sheets, and D. G. Grier, “Computer-generated holographic optical tweezer arrays,” Rev. Sci. Instrum. 72, 1810 (2001).
[CrossRef]

E. R. Dufresne and D. G. Grier, “Optical tweezer arrays and optical substrates created with diffractive optics,” Rev. Sci. Instrum. 69, 1974 (1998).
[CrossRef]

Grover, C.

S. Grover, A. Skirtach, R. Gauther, and C. Grover, “Automated single-cell sorting system based on optical trapping, ” J. Biomed. Opt. 6, 14 (2001).
[CrossRef] [PubMed]

Grover, S.

S. Grover, A. Skirtach, R. Gauther, and C. Grover, “Automated single-cell sorting system based on optical trapping, ” J. Biomed. Opt. 6, 14 (2001).
[CrossRef] [PubMed]

Haist, T.

J. Liesener, M. Reicherter, T. Haist, and H. J. Tiziani, “Multi-functional optical tweezers using computer-generated holograms,” Opt. Commun. 185, 77 (2000).
[CrossRef]

Heckenberg, N.

M. Friese, T. Nieminen, N. Heckenberg, and H. Rubinsztein-Dunlop, “Optical alignment and spinning of laser-trapped microscopic particles,” Nature 394, 348 (1998).
[CrossRef]

Higurashi, E.

E. Higurashi, R. Sawada, and T. Ito, “Optically induced angular alignment of trapped birefringent micro-objects by linear polarization,” Appl. Phys. Lett. 73, 3034 (1998)
[CrossRef]

Holmlin, R. E.

R. E. Holmlin, M. Schiavoni, C. Y. Chen, S. P. Smith, M. G. Prentiss, and G. M. Whitesides, “Light-driven microfabrication: Assembly of multi-component, three-dimensional structures by using optical tweezers,” Angew. Chem. Int. Ed. Engl. 39, 3503 (2000).
[CrossRef] [PubMed]

Ito, T.

E. Higurashi, R. Sawada, and T. Ito, “Optically induced angular alignment of trapped birefringent micro-objects by linear polarization,” Appl. Phys. Lett. 73, 3034 (1998)
[CrossRef]

Kagawa, K.

Kitamura, N.

Kobayashi, Y.

Y. Kobayashi, et al, “Compact High-efficiency Electrically-addressable Phase-only Spatial Light Modulator,” Proc. of SPIE 3951, 158 (2000).
[CrossRef]

Koshioka, M.

Liesener, J.

J. Liesener, M. Reicherter, T. Haist, and H. J. Tiziani, “Multi-functional optical tweezers using computer-generated holograms,” Opt. Commun. 185, 77 (2000).
[CrossRef]

MacDonald, M. P.

M. P. MacDonald, L. Paterson, K. Volke-Sepulveda, J. Arlt, W. Sibbett, and K. Dholakia, “Creation and manipulation of three-dimensional optically trapped structures,” Science 296, 1101 (2002).
[CrossRef] [PubMed]

Masuhara, H.

Misawa, H.

Mogensen, P. C.

Mogensen, P.C.

Nieminen, T.

M. Friese, T. Nieminen, N. Heckenberg, and H. Rubinsztein-Dunlop, “Optical alignment and spinning of laser-trapped microscopic particles,” Nature 394, 348 (1998).
[CrossRef]

Ogura, Y.

Paterson, L.

M. P. MacDonald, L. Paterson, K. Volke-Sepulveda, J. Arlt, W. Sibbett, and K. Dholakia, “Creation and manipulation of three-dimensional optically trapped structures,” Science 296, 1101 (2002).
[CrossRef] [PubMed]

Prentiss, M. G.

R. E. Holmlin, M. Schiavoni, C. Y. Chen, S. P. Smith, M. G. Prentiss, and G. M. Whitesides, “Light-driven microfabrication: Assembly of multi-component, three-dimensional structures by using optical tweezers,” Angew. Chem. Int. Ed. Engl. 39, 3503 (2000).
[CrossRef] [PubMed]

Reicherter, M.

J. Liesener, M. Reicherter, T. Haist, and H. J. Tiziani, “Multi-functional optical tweezers using computer-generated holograms,” Opt. Commun. 185, 77 (2000).
[CrossRef]

Rubinsztein-Dunlop, H.

M. Friese, T. Nieminen, N. Heckenberg, and H. Rubinsztein-Dunlop, “Optical alignment and spinning of laser-trapped microscopic particles,” Nature 394, 348 (1998).
[CrossRef]

Sasaki, K.

Sawada, R.

E. Higurashi, R. Sawada, and T. Ito, “Optically induced angular alignment of trapped birefringent micro-objects by linear polarization,” Appl. Phys. Lett. 73, 3034 (1998)
[CrossRef]

Schiavoni, M.

R. E. Holmlin, M. Schiavoni, C. Y. Chen, S. P. Smith, M. G. Prentiss, and G. M. Whitesides, “Light-driven microfabrication: Assembly of multi-component, three-dimensional structures by using optical tweezers,” Angew. Chem. Int. Ed. Engl. 39, 3503 (2000).
[CrossRef] [PubMed]

Sheets, S. A.

E. R. Dufresne, G. C. Spalding, M. T. Dearing, S. A. Sheets, and D. G. Grier, “Computer-generated holographic optical tweezer arrays,” Rev. Sci. Instrum. 72, 1810 (2001).
[CrossRef]

Sibbett, W.

M. P. MacDonald, L. Paterson, K. Volke-Sepulveda, J. Arlt, W. Sibbett, and K. Dholakia, “Creation and manipulation of three-dimensional optically trapped structures,” Science 296, 1101 (2002).
[CrossRef] [PubMed]

Skirtach, A.

S. Grover, A. Skirtach, R. Gauther, and C. Grover, “Automated single-cell sorting system based on optical trapping, ” J. Biomed. Opt. 6, 14 (2001).
[CrossRef] [PubMed]

Smith, S. P.

R. E. Holmlin, M. Schiavoni, C. Y. Chen, S. P. Smith, M. G. Prentiss, and G. M. Whitesides, “Light-driven microfabrication: Assembly of multi-component, three-dimensional structures by using optical tweezers,” Angew. Chem. Int. Ed. Engl. 39, 3503 (2000).
[CrossRef] [PubMed]

Spalding, G. C.

E. R. Dufresne, G. C. Spalding, M. T. Dearing, S. A. Sheets, and D. G. Grier, “Computer-generated holographic optical tweezer arrays,” Rev. Sci. Instrum. 72, 1810 (2001).
[CrossRef]

Svoboda, K.

K. Svoboda and S. M Block, “Biological applications of optical forces, ”Annu. Rev. Biophys. Biomol. Struct. 23, 247 (1994).
[CrossRef] [PubMed]

Tanida, J.

Tiziani, H. J.

J. Liesener, M. Reicherter, T. Haist, and H. J. Tiziani, “Multi-functional optical tweezers using computer-generated holograms,” Opt. Commun. 185, 77 (2000).
[CrossRef]

Volke-Sepulveda, K.

M. P. MacDonald, L. Paterson, K. Volke-Sepulveda, J. Arlt, W. Sibbett, and K. Dholakia, “Creation and manipulation of three-dimensional optically trapped structures,” Science 296, 1101 (2002).
[CrossRef] [PubMed]

Whitesides, G. M.

R. E. Holmlin, M. Schiavoni, C. Y. Chen, S. P. Smith, M. G. Prentiss, and G. M. Whitesides, “Light-driven microfabrication: Assembly of multi-component, three-dimensional structures by using optical tweezers,” Angew. Chem. Int. Ed. Engl. 39, 3503 (2000).
[CrossRef] [PubMed]

Angew. Chem. Int. Ed. Engl. (1)

R. E. Holmlin, M. Schiavoni, C. Y. Chen, S. P. Smith, M. G. Prentiss, and G. M. Whitesides, “Light-driven microfabrication: Assembly of multi-component, three-dimensional structures by using optical tweezers,” Angew. Chem. Int. Ed. Engl. 39, 3503 (2000).
[CrossRef] [PubMed]

Annu. Rev. Biophys. Biomol. Struct. (1)

K. Svoboda and S. M Block, “Biological applications of optical forces, ”Annu. Rev. Biophys. Biomol. Struct. 23, 247 (1994).
[CrossRef] [PubMed]

Appl. Opt. (3)

Appl. Phys. Lett. (1)

E. Higurashi, R. Sawada, and T. Ito, “Optically induced angular alignment of trapped birefringent micro-objects by linear polarization,” Appl. Phys. Lett. 73, 3034 (1998)
[CrossRef]

J. Biomed. Opt. (1)

S. Grover, A. Skirtach, R. Gauther, and C. Grover, “Automated single-cell sorting system based on optical trapping, ” J. Biomed. Opt. 6, 14 (2001).
[CrossRef] [PubMed]

Nature (1)

M. Friese, T. Nieminen, N. Heckenberg, and H. Rubinsztein-Dunlop, “Optical alignment and spinning of laser-trapped microscopic particles,” Nature 394, 348 (1998).
[CrossRef]

Opt. Commun. (1)

J. Liesener, M. Reicherter, T. Haist, and H. J. Tiziani, “Multi-functional optical tweezers using computer-generated holograms,” Opt. Commun. 185, 77 (2000).
[CrossRef]

Opt. Lett. (3)

Proc. of SPIE (1)

Y. Kobayashi, et al, “Compact High-efficiency Electrically-addressable Phase-only Spatial Light Modulator,” Proc. of SPIE 3951, 158 (2000).
[CrossRef]

Rev. Sci. Instrum. (2)

E. R. Dufresne and D. G. Grier, “Optical tweezer arrays and optical substrates created with diffractive optics,” Rev. Sci. Instrum. 69, 1974 (1998).
[CrossRef]

E. R. Dufresne, G. C. Spalding, M. T. Dearing, S. A. Sheets, and D. G. Grier, “Computer-generated holographic optical tweezer arrays,” Rev. Sci. Instrum. 72, 1810 (2001).
[CrossRef]

Science (1)

M. P. MacDonald, L. Paterson, K. Volke-Sepulveda, J. Arlt, W. Sibbett, and K. Dholakia, “Creation and manipulation of three-dimensional optically trapped structures,” Science 296, 1101 (2002).
[CrossRef] [PubMed]

Other (1)

J. Glückstad, “Phase contrast imaging,” U.S. patent 6,011,874 (January 4 2000).

Supplementary Material (1)

» Media 1: MPG (2306 KB)     

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Figures (3)

Figure 1:
Figure 1:

The schematic diagram of the experimental set-up for the dynamic multiple-beam phase contrast-based optical tweezers system using a reflection-mode phase-only spatial light modulator.

Figure 2.
Figure 2.

Image (a) shows a gray-scale representation of the phase pattern that is addressed on the SLM to generate a 4×4 optical trap and image (b) shows the efficient trapping of 16 particles (2 μm in diameter).

Figure 3.
Figure 3.

An image sequence showing the dynamic rotation of eight-trapped polystyrene beads (2 μm in diameter) in the phase-contrast-generated optical traps. The outer six particles rotate clock-wise 1/8 of a full rotation while the inner two particles rotate counter clock-wise nearly one full rotation. [Media 1]

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