Abstract

We present a new application and current results for extending depth of field using wave front coding. A cubic phase plate is used to code wave fronts in microscopy resulting in extended depths of field and inexpensive chromatic aberration control. A review of the theory behind cubic phase plate extended depth of field systems is given along with the challenges that are face when applying the theory to microscopy. Current results from the new extended depth of field microscope systems are shown

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References

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  1. S. Bradburn, E. R. Dowski, Jr. and W. Thomas Cathey, "Realizations of focus invariance in optical-digital systems with wave-front coding," Appl. Opt. 36, 9157-9166 (1997).
    [CrossRef]
  2. E. R. Dowski, Jr. and W. Thomas Cathey, "Extended depth of field through wave-front coding," Appl. Opt. 34, 1859-1866 (1995).
    [CrossRef] [PubMed]
  3. Imaging Systems Laboratory, www.colorado.edu/isl
  4. H. B. Wach, E. R. Dowski, Jr., and W. Thomas Cathey, Control of chromatic focal shift through wavefront coding," Appl. Opt. 37, 5359-5367 (1998).
    [CrossRef]

Other

S. Bradburn, E. R. Dowski, Jr. and W. Thomas Cathey, "Realizations of focus invariance in optical-digital systems with wave-front coding," Appl. Opt. 36, 9157-9166 (1997).
[CrossRef]

E. R. Dowski, Jr. and W. Thomas Cathey, "Extended depth of field through wave-front coding," Appl. Opt. 34, 1859-1866 (1995).
[CrossRef] [PubMed]

Imaging Systems Laboratory, www.colorado.edu/isl

H. B. Wach, E. R. Dowski, Jr., and W. Thomas Cathey, Control of chromatic focal shift through wavefront coding," Appl. Opt. 37, 5359-5367 (1998).
[CrossRef]

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Figures (7)

Figure 1.
Figure 1.

General raytraces of standard (left) and cubic phase plate (right) imaging systems. Rays are traced from exit pupil plane to just past focal plane.

Figure 2.
Figure 2.

Images of standard point spread functions (PSFs) with zero waves of defocus (a) and 10 waves of defocus (b) next to images of cubic phase plate system PSFs with zero waves of defocus (c) and 10 waves of defocus (d).

Figure 3.
Figure 3.

Standard and cubic phase plate imaging of a feather, 3× magnification: (a) standard imaging system with focus in front, (b) standard imaging system with focus in back, (c) cubic phase plate imaging system intermediate image, and (d) cubic phase plate imaging system image after digital post processing

Figure 4.
Figure 4.

(a) Standard system image, (b) intermediate cubic phase plate system image, and (c) filtered cubic phase plate system image of human hair, 10× magnification. The average thickness of the hairs is 65 μm.

Figure 5.
Figure 5.

Demonstration of extending depth of field to correct for chromatic aberration. Example blue, green, and red wavelengths focus at different points, but when the depth of field for each wavelength is extended, the overlapping depths result in an overall, chromatic aberration free extended depth of field image

Figure 6.
Figure 6.

Blue, green, and red images of salt taken (separately) with a standard 5× microscope imaging system.

Figure 7.
Figure 7.

Combined blue, green, and red images of salt taken with (left) standard 5× microscope imaging system and (right) 5× extended depth of field microscope imaging system.

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