Abstract

We demonstrate an integrated holographic optical tweezers system with double-helix point spread function (DH-PSF) imaging for high precision three-dimensional multi-particle tracking. The tweezers system allows for the creation and control of multiple optical traps in three-dimensions, while the DH-PSF allows for high precision, 3D, multiple-particle tracking in a wide field. The integrated system is suitable for particles emitting/scattering either coherent or incoherent light and is easily adaptable to existing holographic tweezers systems. We demonstrate simultaneous tracking of multiple micro-manipulated particles and perform quantitative estimation of the lateral and axial forces in an optical trap by measuring the fluid drag force exerted on the particles. The system is thus capable of unveiling complex 3D force landscapes that make it suitable for quantitative studies of interactions in colloidal systems, biological materials, and a variety of soft matter systems.

© 2011 OSA

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    [CrossRef]
  2. K. Svoboda and S. M. Block, “Biological applications of optical forces,” Annu. Rev. Biophys. Biomol. Struct. 23(1), 247–285 (1994).
    [CrossRef] [PubMed]
  3. C. N. Cohen-Tannoudji and W. D. Phillips, “New Mechanisms for Laser Cooling,” Phys. Today 43(10), 33–40 (1990).
    [CrossRef]
  4. S. Chu, “Laser Trapping of Neutral Particles,” Sci. Am. 266(2), 71–76 (1992).
    [CrossRef]
  5. J. Liesener, M. Reicherter, T. Haist, and H. J. Tiziani, “Multi-functional optical tweezers using computer generated holograms,” Opt. Commun. 185(1-3), 77–82 (2000).
    [CrossRef]
  6. D. G. Grier, “A revolution in optical manipulation,” Nature 424(6950), 810–816 (2003).
    [CrossRef] [PubMed]
  7. A. Pralle, M. Prummer, E. L. Florin, E. H. K. Stelzer, and J. K. H. Hörber, “Three-dimensional high-resolution particle tracking for optical tweezers by forward scattered light,” Microsc. Res. Tech. 44(5), 378–386 (1999).
    [CrossRef] [PubMed]
  8. T. T. Perkins, “Optical traps for single molecule biophysics: a primer,” Laser Photonics Rev. 3(1-2), 203–220 (2009).
    [CrossRef]
  9. S. H. Lee and D. G. Grier, “Holographic microscopy of holographically trapped three-dimensional structures,” Opt. Express 15(4), 1505–1512 (2007).
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    [CrossRef] [PubMed]
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  12. A. Greengard, Y. Y. Schechner, and R. Piestun, “Depth from diffracted rotation,” Opt. Lett. 31(2), 181–183 (2006).
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  13. S. R. P. Pavani and R. Piestun, “Three dimensional tracking of fluorescent microparticles using a photon-limited double-helix response system,” Opt. Express 16(26), 22048–22057 (2008).
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  16. S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A. 106(9), 2995–2999 (2009).
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  17. S. R. P. Pavani, J. G. DeLuca, and R. Piestun, “Polarization sensitive, three-dimensional, single-molecule imaging of cells with a double-helix system,” Opt. Express 17(22), 19644–19655 (2009).
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  21. K. Schutze, I. Becker, K. F. Becker, S. Thalhammer, R. Stark, W. M. Heckl, M. Bohm, and H. Posl, “Cut out or poke in - the key to the world of single genes: Laser micromanipulation as a valuable tool on the look-out for the origin of disease,” Genet. Anal. Biomol. Eng. 14, 1–8 (1997).
    [CrossRef]
  22. K. Schütze, H. Pösl, and G. Lahr, “Laser micromanipulation systems as universal tools in cellular and molecular biology and in medicine,” Cell. Mol. Biol. (Noisy-le-grand) 44(5), 735–746 (1998).
  23. P. T. Korda, G. C. Spalding, and D. G. Grier, “Evolution of a colloidal critical state in an optical pinning potential landscape,” Phys. Rev. B 66(2), 024504 (2002).
    [CrossRef]
  24. D. G. Grier, “Optical tweezers in colloid and interface science,” Curr. Opin. Colloid Interface Sci. 2(3), 264–270 (1997).
    [CrossRef]
  25. S. Anand, R. Trivedi, G. Stockdale, and I. Smalyukh, “Non-contact optical control of multiple particles and defects using holographic optical trapping with phase-only liquid crystal spatial light modulator,” Proc. SPIE 7232, 723208 (2009).
    [CrossRef]
  26. S. Keen, J. Leach, G. Gibson, and M. J. Padgett, “Comparison of a high-speed camera and a quadrant detector for measuring displacements in optical tweezers,” J. Opt. A, Pure Appl. Opt. 9(8), S264–S266 (2007).
    [CrossRef]
  27. I. I. Smalyukh, A. V. Kachynski, A. N. Kuzmin, and P. N. Prasad, “Laser trapping in anisoptropic fluids and polarization-controlled particle dynamics,” Proc. Natl. Acad. Sci. U.S.A. 103(48), 18048–18053 (2006).
    [CrossRef] [PubMed]
  28. S. Quirin, G. Grover, and R. Piestun, “Double-Helix PSF Microscopy with a Phase Mask for Efficient Photon Collection,” to appear in Novel Techniques in Microscopy, OSA Technical Digest (CD) (Optical Society of America, 2011).
  29. Q. Liu, Y. Cui, D. Gardner, X. Li, S. He, and I. I. Smalyukh, “Self-alignment of plasmonic gold nanorods in reconfigurable anisotropic fluids for tunable bulk metamaterial applications,” Nano Lett. 10(4), 1347–1353 (2010).
    [CrossRef] [PubMed]
  30. C. P. Lapointe, T. G. Mason, and I. I. Smalyukh, “Shape-controlled colloidal interactions in nematic liquid crystals,” Science 326(5956), 1083–1086 (2009).
    [CrossRef] [PubMed]

2010

M. A. Thompson, M. D. Lew, M. Badieirostami, and W. E. Moerner, “Localizing and tracking single nanoscale emitters in three dimensions with high spatiotemporal resolution using a double-helix point spread function,” Nano Lett. 10, 211–218 (2010).
[CrossRef]

Q. Liu, Y. Cui, D. Gardner, X. Li, S. He, and I. I. Smalyukh, “Self-alignment of plasmonic gold nanorods in reconfigurable anisotropic fluids for tunable bulk metamaterial applications,” Nano Lett. 10(4), 1347–1353 (2010).
[CrossRef] [PubMed]

R. Bowman, G. Gibson, and M. Padgett, “Particle tracking stereomicroscopy in optical tweezers: control of trap shape,” Opt. Express 18(11), 11785–11790 (2010).
[CrossRef] [PubMed]

G. Grover, S. R. Pavani, and R. Piestun, “Performance limits on three-dimensional particle localization in photon-limited microscopy,” Opt. Lett. 35(19), 3306–3308 (2010).
[CrossRef] [PubMed]

2009

S. Anand, R. Trivedi, G. Stockdale, and I. Smalyukh, “Non-contact optical control of multiple particles and defects using holographic optical trapping with phase-only liquid crystal spatial light modulator,” Proc. SPIE 7232, 723208 (2009).
[CrossRef]

S. R. P. Pavani, J. G. DeLuca, and R. Piestun, “Polarization sensitive, three-dimensional, single-molecule imaging of cells with a double-helix system,” Opt. Express 17(22), 19644–19655 (2009).
[CrossRef] [PubMed]

C. P. Lapointe, T. G. Mason, and I. I. Smalyukh, “Shape-controlled colloidal interactions in nematic liquid crystals,” Science 326(5956), 1083–1086 (2009).
[CrossRef] [PubMed]

S. R. P. Pavani, A. Greengard, and R. Piestun, “Three-dimensional localization with nanometer accuracy using a detector-limited double-helix point spread function system,” Appl. Phys. Lett. 95(2), 021103 (2009).
[CrossRef]

S. R. P. Pavani and R. Piestun, “3D microscopy with a double-helix point spread function,” Proc. SPIE 7184, 718401 (2009).

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A. 106(9), 2995–2999 (2009).
[CrossRef] [PubMed]

T. T. Perkins, “Optical traps for single molecule biophysics: a primer,” Laser Photonics Rev. 3(1-2), 203–220 (2009).
[CrossRef]

2008

2007

S. Keen, J. Leach, G. Gibson, and M. J. Padgett, “Comparison of a high-speed camera and a quadrant detector for measuring displacements in optical tweezers,” J. Opt. A, Pure Appl. Opt. 9(8), S264–S266 (2007).
[CrossRef]

S. H. Lee and D. G. Grier, “Holographic microscopy of holographically trapped three-dimensional structures,” Opt. Express 15(4), 1505–1512 (2007).
[CrossRef] [PubMed]

2006

I. I. Smalyukh, A. V. Kachynski, A. N. Kuzmin, and P. N. Prasad, “Laser trapping in anisoptropic fluids and polarization-controlled particle dynamics,” Proc. Natl. Acad. Sci. U.S.A. 103(48), 18048–18053 (2006).
[CrossRef] [PubMed]

A. Greengard, Y. Y. Schechner, and R. Piestun, “Depth from diffracted rotation,” Opt. Lett. 31(2), 181–183 (2006).
[CrossRef] [PubMed]

2003

D. G. Grier, “A revolution in optical manipulation,” Nature 424(6950), 810–816 (2003).
[CrossRef] [PubMed]

2002

P. T. Korda, G. C. Spalding, and D. G. Grier, “Evolution of a colloidal critical state in an optical pinning potential landscape,” Phys. Rev. B 66(2), 024504 (2002).
[CrossRef]

2000

J. Liesener, M. Reicherter, T. Haist, and H. J. Tiziani, “Multi-functional optical tweezers using computer generated holograms,” Opt. Commun. 185(1-3), 77–82 (2000).
[CrossRef]

1999

A. Pralle, M. Prummer, E. L. Florin, E. H. K. Stelzer, and J. K. H. Hörber, “Three-dimensional high-resolution particle tracking for optical tweezers by forward scattered light,” Microsc. Res. Tech. 44(5), 378–386 (1999).
[CrossRef] [PubMed]

1998

K. Schütze, H. Pösl, and G. Lahr, “Laser micromanipulation systems as universal tools in cellular and molecular biology and in medicine,” Cell. Mol. Biol. (Noisy-le-grand) 44(5), 735–746 (1998).

1997

D. G. Grier, “Optical tweezers in colloid and interface science,” Curr. Opin. Colloid Interface Sci. 2(3), 264–270 (1997).
[CrossRef]

K. Schutze, I. Becker, K. F. Becker, S. Thalhammer, R. Stark, W. M. Heckl, M. Bohm, and H. Posl, “Cut out or poke in - the key to the world of single genes: Laser micromanipulation as a valuable tool on the look-out for the origin of disease,” Genet. Anal. Biomol. Eng. 14, 1–8 (1997).
[CrossRef]

1994

K. Svoboda and S. M. Block, “Biological applications of optical forces,” Annu. Rev. Biophys. Biomol. Struct. 23(1), 247–285 (1994).
[CrossRef] [PubMed]

1992

S. Chu, “Laser Trapping of Neutral Particles,” Sci. Am. 266(2), 71–76 (1992).
[CrossRef]

1990

C. N. Cohen-Tannoudji and W. D. Phillips, “New Mechanisms for Laser Cooling,” Phys. Today 43(10), 33–40 (1990).
[CrossRef]

1970

A. Ashkin, “Acceleration and trapping of particles by radiation pressure,” Phys. Rev. Lett. 24(4), 156–159 (1970).
[CrossRef]

Anand, S.

S. Anand, R. Trivedi, G. Stockdale, and I. Smalyukh, “Non-contact optical control of multiple particles and defects using holographic optical trapping with phase-only liquid crystal spatial light modulator,” Proc. SPIE 7232, 723208 (2009).
[CrossRef]

Ashkin, A.

A. Ashkin, “Acceleration and trapping of particles by radiation pressure,” Phys. Rev. Lett. 24(4), 156–159 (1970).
[CrossRef]

Badieirostami, M.

M. A. Thompson, M. D. Lew, M. Badieirostami, and W. E. Moerner, “Localizing and tracking single nanoscale emitters in three dimensions with high spatiotemporal resolution using a double-helix point spread function,” Nano Lett. 10, 211–218 (2010).
[CrossRef]

Becker, I.

K. Schutze, I. Becker, K. F. Becker, S. Thalhammer, R. Stark, W. M. Heckl, M. Bohm, and H. Posl, “Cut out or poke in - the key to the world of single genes: Laser micromanipulation as a valuable tool on the look-out for the origin of disease,” Genet. Anal. Biomol. Eng. 14, 1–8 (1997).
[CrossRef]

Becker, K. F.

K. Schutze, I. Becker, K. F. Becker, S. Thalhammer, R. Stark, W. M. Heckl, M. Bohm, and H. Posl, “Cut out or poke in - the key to the world of single genes: Laser micromanipulation as a valuable tool on the look-out for the origin of disease,” Genet. Anal. Biomol. Eng. 14, 1–8 (1997).
[CrossRef]

Biteen, J. S.

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A. 106(9), 2995–2999 (2009).
[CrossRef] [PubMed]

Block, S. M.

K. Svoboda and S. M. Block, “Biological applications of optical forces,” Annu. Rev. Biophys. Biomol. Struct. 23(1), 247–285 (1994).
[CrossRef] [PubMed]

Bohm, M.

K. Schutze, I. Becker, K. F. Becker, S. Thalhammer, R. Stark, W. M. Heckl, M. Bohm, and H. Posl, “Cut out or poke in - the key to the world of single genes: Laser micromanipulation as a valuable tool on the look-out for the origin of disease,” Genet. Anal. Biomol. Eng. 14, 1–8 (1997).
[CrossRef]

Bowman, R.

Chu, S.

S. Chu, “Laser Trapping of Neutral Particles,” Sci. Am. 266(2), 71–76 (1992).
[CrossRef]

Cohen-Tannoudji, C. N.

C. N. Cohen-Tannoudji and W. D. Phillips, “New Mechanisms for Laser Cooling,” Phys. Today 43(10), 33–40 (1990).
[CrossRef]

Cui, Y.

Q. Liu, Y. Cui, D. Gardner, X. Li, S. He, and I. I. Smalyukh, “Self-alignment of plasmonic gold nanorods in reconfigurable anisotropic fluids for tunable bulk metamaterial applications,” Nano Lett. 10(4), 1347–1353 (2010).
[CrossRef] [PubMed]

Dam, J. S.

DeLuca, J. G.

Florin, E. L.

A. Pralle, M. Prummer, E. L. Florin, E. H. K. Stelzer, and J. K. H. Hörber, “Three-dimensional high-resolution particle tracking for optical tweezers by forward scattered light,” Microsc. Res. Tech. 44(5), 378–386 (1999).
[CrossRef] [PubMed]

Gardner, D.

Q. Liu, Y. Cui, D. Gardner, X. Li, S. He, and I. I. Smalyukh, “Self-alignment of plasmonic gold nanorods in reconfigurable anisotropic fluids for tunable bulk metamaterial applications,” Nano Lett. 10(4), 1347–1353 (2010).
[CrossRef] [PubMed]

Gibson, G.

R. Bowman, G. Gibson, and M. Padgett, “Particle tracking stereomicroscopy in optical tweezers: control of trap shape,” Opt. Express 18(11), 11785–11790 (2010).
[CrossRef] [PubMed]

S. Keen, J. Leach, G. Gibson, and M. J. Padgett, “Comparison of a high-speed camera and a quadrant detector for measuring displacements in optical tweezers,” J. Opt. A, Pure Appl. Opt. 9(8), S264–S266 (2007).
[CrossRef]

Glückstad, J.

Greengard, A.

S. R. P. Pavani, A. Greengard, and R. Piestun, “Three-dimensional localization with nanometer accuracy using a detector-limited double-helix point spread function system,” Appl. Phys. Lett. 95(2), 021103 (2009).
[CrossRef]

A. Greengard, Y. Y. Schechner, and R. Piestun, “Depth from diffracted rotation,” Opt. Lett. 31(2), 181–183 (2006).
[CrossRef] [PubMed]

Grier, D. G.

S. H. Lee and D. G. Grier, “Holographic microscopy of holographically trapped three-dimensional structures,” Opt. Express 15(4), 1505–1512 (2007).
[CrossRef] [PubMed]

D. G. Grier, “A revolution in optical manipulation,” Nature 424(6950), 810–816 (2003).
[CrossRef] [PubMed]

P. T. Korda, G. C. Spalding, and D. G. Grier, “Evolution of a colloidal critical state in an optical pinning potential landscape,” Phys. Rev. B 66(2), 024504 (2002).
[CrossRef]

D. G. Grier, “Optical tweezers in colloid and interface science,” Curr. Opin. Colloid Interface Sci. 2(3), 264–270 (1997).
[CrossRef]

Grover, G.

Haist, T.

J. Liesener, M. Reicherter, T. Haist, and H. J. Tiziani, “Multi-functional optical tweezers using computer generated holograms,” Opt. Commun. 185(1-3), 77–82 (2000).
[CrossRef]

He, S.

Q. Liu, Y. Cui, D. Gardner, X. Li, S. He, and I. I. Smalyukh, “Self-alignment of plasmonic gold nanorods in reconfigurable anisotropic fluids for tunable bulk metamaterial applications,” Nano Lett. 10(4), 1347–1353 (2010).
[CrossRef] [PubMed]

Heckl, W. M.

K. Schutze, I. Becker, K. F. Becker, S. Thalhammer, R. Stark, W. M. Heckl, M. Bohm, and H. Posl, “Cut out or poke in - the key to the world of single genes: Laser micromanipulation as a valuable tool on the look-out for the origin of disease,” Genet. Anal. Biomol. Eng. 14, 1–8 (1997).
[CrossRef]

Hörber, J. K. H.

A. Pralle, M. Prummer, E. L. Florin, E. H. K. Stelzer, and J. K. H. Hörber, “Three-dimensional high-resolution particle tracking for optical tweezers by forward scattered light,” Microsc. Res. Tech. 44(5), 378–386 (1999).
[CrossRef] [PubMed]

Kachynski, A. V.

I. I. Smalyukh, A. V. Kachynski, A. N. Kuzmin, and P. N. Prasad, “Laser trapping in anisoptropic fluids and polarization-controlled particle dynamics,” Proc. Natl. Acad. Sci. U.S.A. 103(48), 18048–18053 (2006).
[CrossRef] [PubMed]

Keen, S.

S. Keen, J. Leach, G. Gibson, and M. J. Padgett, “Comparison of a high-speed camera and a quadrant detector for measuring displacements in optical tweezers,” J. Opt. A, Pure Appl. Opt. 9(8), S264–S266 (2007).
[CrossRef]

Korda, P. T.

P. T. Korda, G. C. Spalding, and D. G. Grier, “Evolution of a colloidal critical state in an optical pinning potential landscape,” Phys. Rev. B 66(2), 024504 (2002).
[CrossRef]

Kuzmin, A. N.

I. I. Smalyukh, A. V. Kachynski, A. N. Kuzmin, and P. N. Prasad, “Laser trapping in anisoptropic fluids and polarization-controlled particle dynamics,” Proc. Natl. Acad. Sci. U.S.A. 103(48), 18048–18053 (2006).
[CrossRef] [PubMed]

Lahr, G.

K. Schütze, H. Pösl, and G. Lahr, “Laser micromanipulation systems as universal tools in cellular and molecular biology and in medicine,” Cell. Mol. Biol. (Noisy-le-grand) 44(5), 735–746 (1998).

Lapointe, C. P.

C. P. Lapointe, T. G. Mason, and I. I. Smalyukh, “Shape-controlled colloidal interactions in nematic liquid crystals,” Science 326(5956), 1083–1086 (2009).
[CrossRef] [PubMed]

Leach, J.

S. Keen, J. Leach, G. Gibson, and M. J. Padgett, “Comparison of a high-speed camera and a quadrant detector for measuring displacements in optical tweezers,” J. Opt. A, Pure Appl. Opt. 9(8), S264–S266 (2007).
[CrossRef]

Lee, S. H.

Lew, M. D.

M. A. Thompson, M. D. Lew, M. Badieirostami, and W. E. Moerner, “Localizing and tracking single nanoscale emitters in three dimensions with high spatiotemporal resolution using a double-helix point spread function,” Nano Lett. 10, 211–218 (2010).
[CrossRef]

Li, X.

Q. Liu, Y. Cui, D. Gardner, X. Li, S. He, and I. I. Smalyukh, “Self-alignment of plasmonic gold nanorods in reconfigurable anisotropic fluids for tunable bulk metamaterial applications,” Nano Lett. 10(4), 1347–1353 (2010).
[CrossRef] [PubMed]

Liesener, J.

J. Liesener, M. Reicherter, T. Haist, and H. J. Tiziani, “Multi-functional optical tweezers using computer generated holograms,” Opt. Commun. 185(1-3), 77–82 (2000).
[CrossRef]

Liu, N.

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A. 106(9), 2995–2999 (2009).
[CrossRef] [PubMed]

Liu, Q.

Q. Liu, Y. Cui, D. Gardner, X. Li, S. He, and I. I. Smalyukh, “Self-alignment of plasmonic gold nanorods in reconfigurable anisotropic fluids for tunable bulk metamaterial applications,” Nano Lett. 10(4), 1347–1353 (2010).
[CrossRef] [PubMed]

Lord, S. J.

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A. 106(9), 2995–2999 (2009).
[CrossRef] [PubMed]

Mason, T. G.

C. P. Lapointe, T. G. Mason, and I. I. Smalyukh, “Shape-controlled colloidal interactions in nematic liquid crystals,” Science 326(5956), 1083–1086 (2009).
[CrossRef] [PubMed]

Moerner, W. E.

M. A. Thompson, M. D. Lew, M. Badieirostami, and W. E. Moerner, “Localizing and tracking single nanoscale emitters in three dimensions with high spatiotemporal resolution using a double-helix point spread function,” Nano Lett. 10, 211–218 (2010).
[CrossRef]

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A. 106(9), 2995–2999 (2009).
[CrossRef] [PubMed]

Padgett, M.

Padgett, M. J.

S. Keen, J. Leach, G. Gibson, and M. J. Padgett, “Comparison of a high-speed camera and a quadrant detector for measuring displacements in optical tweezers,” J. Opt. A, Pure Appl. Opt. 9(8), S264–S266 (2007).
[CrossRef]

Palima, D.

Pavani, S. R.

Pavani, S. R. P.

S. R. P. Pavani and R. Piestun, “3D microscopy with a double-helix point spread function,” Proc. SPIE 7184, 718401 (2009).

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A. 106(9), 2995–2999 (2009).
[CrossRef] [PubMed]

S. R. P. Pavani, J. G. DeLuca, and R. Piestun, “Polarization sensitive, three-dimensional, single-molecule imaging of cells with a double-helix system,” Opt. Express 17(22), 19644–19655 (2009).
[CrossRef] [PubMed]

S. R. P. Pavani, A. Greengard, and R. Piestun, “Three-dimensional localization with nanometer accuracy using a detector-limited double-helix point spread function system,” Appl. Phys. Lett. 95(2), 021103 (2009).
[CrossRef]

S. R. P. Pavani and R. Piestun, “Three dimensional tracking of fluorescent microparticles using a photon-limited double-helix response system,” Opt. Express 16(26), 22048–22057 (2008).
[CrossRef] [PubMed]

Perch-Nielsen, I. R.

Perkins, T. T.

T. T. Perkins, “Optical traps for single molecule biophysics: a primer,” Laser Photonics Rev. 3(1-2), 203–220 (2009).
[CrossRef]

Phillips, W. D.

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Pralle, A.

A. Pralle, M. Prummer, E. L. Florin, E. H. K. Stelzer, and J. K. H. Hörber, “Three-dimensional high-resolution particle tracking for optical tweezers by forward scattered light,” Microsc. Res. Tech. 44(5), 378–386 (1999).
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A. Pralle, M. Prummer, E. L. Florin, E. H. K. Stelzer, and J. K. H. Hörber, “Three-dimensional high-resolution particle tracking for optical tweezers by forward scattered light,” Microsc. Res. Tech. 44(5), 378–386 (1999).
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K. Schutze, I. Becker, K. F. Becker, S. Thalhammer, R. Stark, W. M. Heckl, M. Bohm, and H. Posl, “Cut out or poke in - the key to the world of single genes: Laser micromanipulation as a valuable tool on the look-out for the origin of disease,” Genet. Anal. Biomol. Eng. 14, 1–8 (1997).
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K. Schütze, H. Pösl, and G. Lahr, “Laser micromanipulation systems as universal tools in cellular and molecular biology and in medicine,” Cell. Mol. Biol. (Noisy-le-grand) 44(5), 735–746 (1998).

Smalyukh, I.

S. Anand, R. Trivedi, G. Stockdale, and I. Smalyukh, “Non-contact optical control of multiple particles and defects using holographic optical trapping with phase-only liquid crystal spatial light modulator,” Proc. SPIE 7232, 723208 (2009).
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Smalyukh, I. I.

Q. Liu, Y. Cui, D. Gardner, X. Li, S. He, and I. I. Smalyukh, “Self-alignment of plasmonic gold nanorods in reconfigurable anisotropic fluids for tunable bulk metamaterial applications,” Nano Lett. 10(4), 1347–1353 (2010).
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C. P. Lapointe, T. G. Mason, and I. I. Smalyukh, “Shape-controlled colloidal interactions in nematic liquid crystals,” Science 326(5956), 1083–1086 (2009).
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I. I. Smalyukh, A. V. Kachynski, A. N. Kuzmin, and P. N. Prasad, “Laser trapping in anisoptropic fluids and polarization-controlled particle dynamics,” Proc. Natl. Acad. Sci. U.S.A. 103(48), 18048–18053 (2006).
[CrossRef] [PubMed]

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P. T. Korda, G. C. Spalding, and D. G. Grier, “Evolution of a colloidal critical state in an optical pinning potential landscape,” Phys. Rev. B 66(2), 024504 (2002).
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K. Schutze, I. Becker, K. F. Becker, S. Thalhammer, R. Stark, W. M. Heckl, M. Bohm, and H. Posl, “Cut out or poke in - the key to the world of single genes: Laser micromanipulation as a valuable tool on the look-out for the origin of disease,” Genet. Anal. Biomol. Eng. 14, 1–8 (1997).
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A. Pralle, M. Prummer, E. L. Florin, E. H. K. Stelzer, and J. K. H. Hörber, “Three-dimensional high-resolution particle tracking for optical tweezers by forward scattered light,” Microsc. Res. Tech. 44(5), 378–386 (1999).
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[CrossRef] [PubMed]

Tiziani, H. J.

J. Liesener, M. Reicherter, T. Haist, and H. J. Tiziani, “Multi-functional optical tweezers using computer generated holograms,” Opt. Commun. 185(1-3), 77–82 (2000).
[CrossRef]

Trivedi, R.

S. Anand, R. Trivedi, G. Stockdale, and I. Smalyukh, “Non-contact optical control of multiple particles and defects using holographic optical trapping with phase-only liquid crystal spatial light modulator,” Proc. SPIE 7232, 723208 (2009).
[CrossRef]

Twieg, R. J.

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A. 106(9), 2995–2999 (2009).
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Annu. Rev. Biophys. Biomol. Struct.

K. Svoboda and S. M. Block, “Biological applications of optical forces,” Annu. Rev. Biophys. Biomol. Struct. 23(1), 247–285 (1994).
[CrossRef] [PubMed]

Appl. Phys. Lett.

S. R. P. Pavani, A. Greengard, and R. Piestun, “Three-dimensional localization with nanometer accuracy using a detector-limited double-helix point spread function system,” Appl. Phys. Lett. 95(2), 021103 (2009).
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Cell. Mol. Biol. (Noisy-le-grand)

K. Schütze, H. Pösl, and G. Lahr, “Laser micromanipulation systems as universal tools in cellular and molecular biology and in medicine,” Cell. Mol. Biol. (Noisy-le-grand) 44(5), 735–746 (1998).

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[CrossRef] [PubMed]

Nano Lett.

M. A. Thompson, M. D. Lew, M. Badieirostami, and W. E. Moerner, “Localizing and tracking single nanoscale emitters in three dimensions with high spatiotemporal resolution using a double-helix point spread function,” Nano Lett. 10, 211–218 (2010).
[CrossRef]

Q. Liu, Y. Cui, D. Gardner, X. Li, S. He, and I. I. Smalyukh, “Self-alignment of plasmonic gold nanorods in reconfigurable anisotropic fluids for tunable bulk metamaterial applications,” Nano Lett. 10(4), 1347–1353 (2010).
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[CrossRef]

Opt. Express

Opt. Lett.

Phys. Rev. B

P. T. Korda, G. C. Spalding, and D. G. Grier, “Evolution of a colloidal critical state in an optical pinning potential landscape,” Phys. Rev. B 66(2), 024504 (2002).
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Proc. Natl. Acad. Sci. U.S.A.

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A. 106(9), 2995–2999 (2009).
[CrossRef] [PubMed]

I. I. Smalyukh, A. V. Kachynski, A. N. Kuzmin, and P. N. Prasad, “Laser trapping in anisoptropic fluids and polarization-controlled particle dynamics,” Proc. Natl. Acad. Sci. U.S.A. 103(48), 18048–18053 (2006).
[CrossRef] [PubMed]

Proc. SPIE

S. Anand, R. Trivedi, G. Stockdale, and I. Smalyukh, “Non-contact optical control of multiple particles and defects using holographic optical trapping with phase-only liquid crystal spatial light modulator,” Proc. SPIE 7232, 723208 (2009).
[CrossRef]

S. R. P. Pavani and R. Piestun, “3D microscopy with a double-helix point spread function,” Proc. SPIE 7184, 718401 (2009).

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C. P. Lapointe, T. G. Mason, and I. I. Smalyukh, “Shape-controlled colloidal interactions in nematic liquid crystals,” Science 326(5956), 1083–1086 (2009).
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Other

S. Quirin, G. Grover, and R. Piestun, “Double-Helix PSF Microscopy with a Phase Mask for Efficient Photon Collection,” to appear in Novel Techniques in Microscopy, OSA Technical Digest (CD) (Optical Society of America, 2011).

S. Quirin, S. R. P. Pavani, and R. Piestun, “Pattern Matching Estimator for Precise 3-D Particle Localization with Engineered Point Spread Functions,” in Digital Holography and Three-Dimensional Imaging, OSA Technical Digest (CD) (Optical Society of America, 2010), paper DMC8.

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Figures (4)

Fig. 1
Fig. 1

(a) Schematic showing the experimental setup of the integrated Holographic Optical Tweezer (HOT) system (red beam) and DH-PSF system (green beam). The HOT uses a phase only SLM to phase modulate incoming light to create multiple optical traps in a volume. The DH-PSF has two lobes which rotate with defocus (b). The DH-PSF darkfield phase mask (c) is encoded on a second phase only SLM placed in a Fourier plane of the image to create the DH image used for tracking. The conventional brightfield image (d) and the corresponding off-axis darkfield DH-PSF image with the undiffracted on-axis light suppressed (e) are also shown. The two-lobed responses have different angular orientation for each particle, corresponding to specific axial positions, as can be seen by image defocus in the conventional image.

Fig. 2
Fig. 2

(a) The random 3D thermal motion of an optically trapped 1.1 μm polystyrene bead in water tracked with the DH-PSF. (b) X, Y, and Z histograms of the particle position.

Fig. 3
Fig. 3

3D tracking of four optically trapped particles using the DH-PSF tracking system. The optical traps of the two particles in the background were moved ~1.3 μm axially over 2.5 seconds, while the foreground particle traps remained stationary. Changes in the hologram to move the background traps caused slight changes to the foreground traps, slightly affecting the foreground particles as well.

Fig. 4
Fig. 4

(a) 3D tracking of a particle displaced laterally from an optical trap. The ‘ + ’ indicates the particle position, while the line segments are proportional to the force vectors at the given position. In this experiment the particles do not stay in the lateral plane as they move into the optical trap, but move axially as well. Plots (b,c) show, respectively, the transverse and axial position of the particle as it moved into the optical trap. The circles indicate the measured position. The line indicates the averaged position used for calculating forces. Plots (d,e) show the transverse and axial forces of the optical trap calculated using Stokes Law. The arrows indicate at which particular time the force was experienced.

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