Abstract

Although optical coherence tomography (OCT) can axially resolve and detect reflections from individual cells, there are no reports of imaging cells in the living human retina using OCT. To supplement the axial resolution and sensitivity of OCT with the necessary lateral resolution and speed, we developed a novel spectral domain OCT (SD-OCT) camera based on a free-space parallel illumination architecture and equipped with adaptive optics (AO). Conventional flood illumination, also with AO, was integrated into the camera and provided confirmation of the focus position in the retina with an accuracy of ±10.3 µm. Short bursts of narrow B-scans (100×560 µm) of the living retina were subsequently acquired at 500 Hz during dynamic compensation (up to 14 Hz) that successfully corrected the most significant ocular aberrations across a dilated 6 mm pupil. Camera sensitivity (up to 94 dB) was sufficient for observing reflections from essentially all neural layers of the retina. Signal-to-noise of the detected reflection from the photoreceptor layer was highly sensitive to the level of ocular aberrations and defocus with changes of 11.4 and 13.1 dB (single pass) observed when the ocular aberrations (astigmatism, 3rd order and higher) were corrected and when the focus was shifted by 200 µm (0.54 diopters) in the retina, respectively. The 3D resolution of the B-scans (3.0×3.0×5.7 µm) is the highest reported to date in the living human eye and was sufficient to observe the interface between the inner and outer segments of individual photoreceptor cells, resolved in both lateral and axial dimensions. However, high contrast speckle, which is intrinsic to OCT, was present throughout the AO parallel SD-OCT B-scans and obstructed correlating retinal reflections to cell-sized retinal structures.

©2005 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
High-speed volumetric imaging of cone photoreceptors with adaptive optics spectral-domain optical coherence tomography

Yan Zhang, Barry Cense, Jungtae Rha, Ravi S. Jonnal, Weihua Gao, Robert J. Zawadzki, John S. Werner, Steve Jones, Scot Olivier, and Donald T. Miller
Opt. Express 14(10) 4380-4394 (2006)

Adaptive optics flood-illumination camera for high speed retinal imaging

Jungtae Rha, Ravi S. Jonnal, Karen E. Thorn, Junle Qu, Yan Zhang, and Donald T. Miller
Opt. Express 14(10) 4552-4569 (2006)

Volumetric retinal imaging with ultrahigh-resolution spectral-domain optical coherence tomography and adaptive optics using two broadband light sources

Barry Cense, Eric Koperda, Jeffrey M. Brown, Omer P. Kocaoglu, Weihua Gao, Ravi S. Jonnal, and Donald T. Miller
Opt. Express 17(5) 4095-4111 (2009)

More Recommended Articles

References

  • View by:
  • Article Order
  • |
  • Year
  • |
  • Author
  • |
  • Publication
  1. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, “Optical coherence tomography,” Science 254, 1178–1181 (1991).
    [Crossref] [PubMed]
  2. W. Drexler, U. Morgner, R. K. Ghanta, F. X. Kärtner, J. S. Schuman, and J. G. Fujimoto, “Ultrahigh-resolution ophthalmic optical coherence tomography,” Nature Med. 7, 502–507 (2001).
    [Crossref] [PubMed]
  3. B. Cense, N. A. Nassif, T. C. Chen, M. C. Pierce, S. Yun, B. H. Park, B. E. Bouma, G. J. Tearney, and J. F. de Boer, “Ultrahigh-resolution high-speed retinal imaging using spectral-domain optical coherence tomography,” Opt. Express 12, 2435–2447 (2004), http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-11-2435.
    [Crossref] [PubMed]
  4. M. Wojtkowski, V. J. Srinivasan, T. H. Ko, J. G. Fujimoto, A. Kowalczyk, and J. S. Duker, “Ultrahigh-resolution, high-speed, Fourier domain optical coherence tomography and methods for dispersion compensation,” Opt. Express 12, 2404–2421 (2004), http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-11-2404.
    [Crossref] [PubMed]
  5. R. A. Leitgeb, W. Drexler, A. Unterhuber, B. Hermann, T. Bajraszewski, T. Le, A. Stingl, and A. F. Fercher, “Ultrahigh resolution Fourier domain optical coherence tomography”, Opt. Express 12, 2156–2165 (2004), http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-10-2156.
    [Crossref] [PubMed]
  6. L. N. Thibos, X. Hong, A. Bradley, and X. Cheng, “Statistical variation of aberration structure and image quality in a normal population of healthy eyes,” J. Opt. Soc. Am. A 19, 2329–2348 (2002).
    [Crossref]
  7. R. K. Tyson, Principles of Adaptive Optics (Academic Press, New York, 1998).
  8. J. Liang, D. R. Williams, and D. T. Miller, “Supernormal vision and high resolution retinal imaging through adaptive optics,” J. Opt. Soc. Am. A 14, 2884–2892 (1997).
    [Crossref]
  9. H. Hofer, L. Chen, G. Y. Yoon, B. Singer, Y. Yamauchi, and D. R. Williams, “Improvement in retinal image quality with dynamic correction of the eye’s aberrations,” Opt. Express 8, 631–643 (2001), http://www.opticsexpress.org/abstract.cfm?URI=OPEX-8-11-631.
    [Crossref] [PubMed]
  10. A. Roorda, F. Romero-Borja, W. J. Donnelly, H. Queener, T. J. Hebert, and M. C. W. Campbell, “Adaptive optics scanning laser ophthalmoscopy,” Opt. Express 10, 405–412 (2002), http://www.opticsexpress.org/abstract.cfm?URI=OPEX-10-9-405.
    [PubMed]
  11. D. T. Miller, J. Qu, R. S. Jonnal, and K. Thorn , “Coherence gating and adaptive optics in the eye”, in Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VII, V. V. Tuchin, J. A. Izatt, and J. G. Fujimoto, eds., Proc. SPIE4956, 65–72 (2003).
  12. N. Ling, Y. Zhang, X. Rao, X. Li, C. Wang, Y. Hu, and W. Jiang, “Small table-top adaptive optical systems for human retinal imaging”, in High-Resolution Wavefront Control: Methods, Devices, and Applications IV, J. D. Gonglewski, M. A. Vorontsov, M. T. Gruneisen, S. R. Restaino, and R. K. Tyson, eds., Proc. SPIE4825, 99–108 (2002).
  13. M. Glanc, E. Gendron, F. Lacombe, D. Lafaille, J.-F. Le Gargasson, and P. Léna, “Towards wide-field retinal imaging with adaptive optics,” Opt. Comm. 230, 225–238 (2004).
    [Crossref]
  14. B. Hermann, E. J. Fernández, A. Unterhuber, H. Sattmann, A. F. Fercher, W. Drexler, P. M. Prieto, and P. Artal, “Adaptive-optics ultrahigh-resolution optical coherence tomography,” Opt. Lett. 29, 2142–2144 (2004).
    [Crossref] [PubMed]
  15. B. Grajciar, M. Pircher, A. F. Fercher, and R. A. Leitgeb, “Parallel Fourier domain optical coherence tomography for in vivo measurement of the human eye,” Opt. Express 13, 1131–1137 (2005), http://www.opticsexpress.org/abstract.cfm?URI=OPEX-13-4-1131.
    [Crossref] [PubMed]
  16. Y. Zhang, J. Rha, R. S. Jonnal, and D. T. Miller, “Single shot retinal imaging with AO spectral OCT,” in Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine IX, V. V. Tuchin, J. A. Izatt, and J. G. Fujimoto, eds. Proc. SPIE5690, 548–555 (2005).
  17. ANSI, American National Standard for the Safe Use of Lasers, ANSI Z136.1 (Laser Institute of America, Orlando, FL, 2000).
  18. K. E. Thorn, J. Qu, R. J. Jonnal, and D. T. Miller, “Adaptive optics flood-illuminated camera for high speed retinal imaging,” Invest. Ophthalmol. Vis. Sci. 44, E-Abstract 1002 (2003).
  19. J. Hecht, Understanding Fiber Optics (Prentice Hall, New Jersey, 1998).
  20. W. Jiang and H. Li, “Hartmann-Shack wavefront sensing and control algorithm,” in Adaptive Optics and Optical Structures, J. J. Schulte-in-den-Baeumen and R. K. Tyson, eds., Proc. SPIE1271, 82–93 (1990).
  21. J. Liang, B. Grimm, S. Goelz, and J. Bille, “Objective measurement of the wave aberrations of the human eye using a Hartmann-Shack wavefront sensor,” J. Opt. Soc. Am. A 11, 1949–1957 (1994).
    [Crossref]
  22. D. Malacara, Optical Shop Testing2nd ed. (John Wiley & Sons, New York, 1992), 112–113.
  23. L. N. Thibos, X. Hong, A. Bradley, and R. A. Applegate, “Accuracy and precision of methods to predict the results of subjective refraction from monochromatic wavefront aberration maps,” J. Vis. 4, 329–351 (2004).
    [PubMed]
  24. R. Leitgeb, C. K. Hitzenberger, and A. F. Fercher, “Performance of Fourier domain versus time domain optical coherence tomography,” Opt. Express 11, 889–894 (2003), http://www.opticsexpress.org/abstract.cfm?URI=OPEX-11-8-889.
    [Crossref] [PubMed]
  25. R. J. Zawadzki, B. A. Bower, M. Zhao, M. Sarunic, S. Laut, J. S. Werner, and J. A. Izatt, “Exposure time dependence of image quality in high-speed retinal in vivo Fourier domain OCT,” in Ophthalmic Technologies XV, F. Manns, P. G. Soederberg, A. Ho, B. E. Stuck, and M. Belkin, eds., Proc. SPIE5688, 45–52 (2005).
  26. C. A. Curcio, K. R. Sloan, R. E. Kalina, and A. E. Hendrickson, “Human photoreceptor topography,” J. Comparitive Neurology 292, 497–523 (1990).
    [Crossref]
  27. D. R. Williams, “Topography of the foveal cone mosaic in the living human eye,” Vision Res. 28, 433–454 (1988).
    [Crossref] [PubMed]
  28. N. Nassif, B. Cense, B. H. Park, S. H. Yun, T. C. Chen, B. E. Bouma, G. J. Tearney, and J. F. de Boer, “In vivo human retinal imaging by ultrahigh-speed spectral domain optical coherence tomography,” Opt. Lett. 29, 480–482 (2004).
    [Crossref] [PubMed]
  29. B. Sander, M Larsen, L. Thrane, J. L. Hougaard, and T. M. Jørgensen, “Enhanced optical coherence tomography imaging by multiple scan averaging,” Br. J. Ophthalmol. 89, 207–212 (2004).
    [Crossref]
  30. L. A. Riggs, J. C. Armington, and J.C., “Motions of the retinal image during fixation,” J. Opt. Soc. Am. 44, 315–321 (1954).
    [Crossref] [PubMed]
  31. D. T. Miller, D. R. Williams, G. M. Morris, and J. Liang, “Images of cone photoreceptors in the living human eye,” Vision Res. 36, 1067–1079 (1996).
    [Crossref] [PubMed]

2005 (1)

2004 (8)

M. Glanc, E. Gendron, F. Lacombe, D. Lafaille, J.-F. Le Gargasson, and P. Léna, “Towards wide-field retinal imaging with adaptive optics,” Opt. Comm. 230, 225–238 (2004).
[Crossref]

B. Hermann, E. J. Fernández, A. Unterhuber, H. Sattmann, A. F. Fercher, W. Drexler, P. M. Prieto, and P. Artal, “Adaptive-optics ultrahigh-resolution optical coherence tomography,” Opt. Lett. 29, 2142–2144 (2004).
[Crossref] [PubMed]

L. N. Thibos, X. Hong, A. Bradley, and R. A. Applegate, “Accuracy and precision of methods to predict the results of subjective refraction from monochromatic wavefront aberration maps,” J. Vis. 4, 329–351 (2004).
[PubMed]

B. Cense, N. A. Nassif, T. C. Chen, M. C. Pierce, S. Yun, B. H. Park, B. E. Bouma, G. J. Tearney, and J. F. de Boer, “Ultrahigh-resolution high-speed retinal imaging using spectral-domain optical coherence tomography,” Opt. Express 12, 2435–2447 (2004), http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-11-2435.
[Crossref] [PubMed]

M. Wojtkowski, V. J. Srinivasan, T. H. Ko, J. G. Fujimoto, A. Kowalczyk, and J. S. Duker, “Ultrahigh-resolution, high-speed, Fourier domain optical coherence tomography and methods for dispersion compensation,” Opt. Express 12, 2404–2421 (2004), http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-11-2404.
[Crossref] [PubMed]

R. A. Leitgeb, W. Drexler, A. Unterhuber, B. Hermann, T. Bajraszewski, T. Le, A. Stingl, and A. F. Fercher, “Ultrahigh resolution Fourier domain optical coherence tomography”, Opt. Express 12, 2156–2165 (2004), http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-10-2156.
[Crossref] [PubMed]

N. Nassif, B. Cense, B. H. Park, S. H. Yun, T. C. Chen, B. E. Bouma, G. J. Tearney, and J. F. de Boer, “In vivo human retinal imaging by ultrahigh-speed spectral domain optical coherence tomography,” Opt. Lett. 29, 480–482 (2004).
[Crossref] [PubMed]

B. Sander, M Larsen, L. Thrane, J. L. Hougaard, and T. M. Jørgensen, “Enhanced optical coherence tomography imaging by multiple scan averaging,” Br. J. Ophthalmol. 89, 207–212 (2004).
[Crossref]

2003 (2)

R. Leitgeb, C. K. Hitzenberger, and A. F. Fercher, “Performance of Fourier domain versus time domain optical coherence tomography,” Opt. Express 11, 889–894 (2003), http://www.opticsexpress.org/abstract.cfm?URI=OPEX-11-8-889.
[Crossref] [PubMed]

K. E. Thorn, J. Qu, R. J. Jonnal, and D. T. Miller, “Adaptive optics flood-illuminated camera for high speed retinal imaging,” Invest. Ophthalmol. Vis. Sci. 44, E-Abstract 1002 (2003).

2002 (2)

2001 (2)

1997 (1)

1996 (1)

D. T. Miller, D. R. Williams, G. M. Morris, and J. Liang, “Images of cone photoreceptors in the living human eye,” Vision Res. 36, 1067–1079 (1996).
[Crossref] [PubMed]

1994 (1)

1991 (1)

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, “Optical coherence tomography,” Science 254, 1178–1181 (1991).
[Crossref] [PubMed]

1990 (1)

C. A. Curcio, K. R. Sloan, R. E. Kalina, and A. E. Hendrickson, “Human photoreceptor topography,” J. Comparitive Neurology 292, 497–523 (1990).
[Crossref]

1988 (1)

D. R. Williams, “Topography of the foveal cone mosaic in the living human eye,” Vision Res. 28, 433–454 (1988).
[Crossref] [PubMed]

1954 (1)

Applegate, R. A.

L. N. Thibos, X. Hong, A. Bradley, and R. A. Applegate, “Accuracy and precision of methods to predict the results of subjective refraction from monochromatic wavefront aberration maps,” J. Vis. 4, 329–351 (2004).
[PubMed]

Armington, J. C.

Artal, P.

Bajraszewski, T.

Bille, J.

Bouma, B. E.

Bower, B. A.

R. J. Zawadzki, B. A. Bower, M. Zhao, M. Sarunic, S. Laut, J. S. Werner, and J. A. Izatt, “Exposure time dependence of image quality in high-speed retinal in vivo Fourier domain OCT,” in Ophthalmic Technologies XV, F. Manns, P. G. Soederberg, A. Ho, B. E. Stuck, and M. Belkin, eds., Proc. SPIE5688, 45–52 (2005).

Bradley, A.

L. N. Thibos, X. Hong, A. Bradley, and R. A. Applegate, “Accuracy and precision of methods to predict the results of subjective refraction from monochromatic wavefront aberration maps,” J. Vis. 4, 329–351 (2004).
[PubMed]

L. N. Thibos, X. Hong, A. Bradley, and X. Cheng, “Statistical variation of aberration structure and image quality in a normal population of healthy eyes,” J. Opt. Soc. Am. A 19, 2329–2348 (2002).
[Crossref]

Campbell, M. C. W.

Cense, B.

Chang, W.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, “Optical coherence tomography,” Science 254, 1178–1181 (1991).
[Crossref] [PubMed]

Chen, L.

Chen, T. C.

Cheng, X.

Curcio, C. A.

C. A. Curcio, K. R. Sloan, R. E. Kalina, and A. E. Hendrickson, “Human photoreceptor topography,” J. Comparitive Neurology 292, 497–523 (1990).
[Crossref]

de Boer, J. F.

Donnelly, W. J.

Drexler, W.

Duker, J. S.

Fercher, A. F.

Fernández, E. J.

Flotte, T.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, “Optical coherence tomography,” Science 254, 1178–1181 (1991).
[Crossref] [PubMed]

Fujimoto, J. G.

M. Wojtkowski, V. J. Srinivasan, T. H. Ko, J. G. Fujimoto, A. Kowalczyk, and J. S. Duker, “Ultrahigh-resolution, high-speed, Fourier domain optical coherence tomography and methods for dispersion compensation,” Opt. Express 12, 2404–2421 (2004), http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-11-2404.
[Crossref] [PubMed]

W. Drexler, U. Morgner, R. K. Ghanta, F. X. Kärtner, J. S. Schuman, and J. G. Fujimoto, “Ultrahigh-resolution ophthalmic optical coherence tomography,” Nature Med. 7, 502–507 (2001).
[Crossref] [PubMed]

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, “Optical coherence tomography,” Science 254, 1178–1181 (1991).
[Crossref] [PubMed]

Gendron, E.

M. Glanc, E. Gendron, F. Lacombe, D. Lafaille, J.-F. Le Gargasson, and P. Léna, “Towards wide-field retinal imaging with adaptive optics,” Opt. Comm. 230, 225–238 (2004).
[Crossref]

Ghanta, R. K.

W. Drexler, U. Morgner, R. K. Ghanta, F. X. Kärtner, J. S. Schuman, and J. G. Fujimoto, “Ultrahigh-resolution ophthalmic optical coherence tomography,” Nature Med. 7, 502–507 (2001).
[Crossref] [PubMed]

Glanc, M.

M. Glanc, E. Gendron, F. Lacombe, D. Lafaille, J.-F. Le Gargasson, and P. Léna, “Towards wide-field retinal imaging with adaptive optics,” Opt. Comm. 230, 225–238 (2004).
[Crossref]

Goelz, S.

Grajciar, B.

Gregory, K.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, “Optical coherence tomography,” Science 254, 1178–1181 (1991).
[Crossref] [PubMed]

Grimm, B.

Hebert, T. J.

Hecht, J.

J. Hecht, Understanding Fiber Optics (Prentice Hall, New Jersey, 1998).

Hee, M. R.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, “Optical coherence tomography,” Science 254, 1178–1181 (1991).
[Crossref] [PubMed]

Hendrickson, A. E.

C. A. Curcio, K. R. Sloan, R. E. Kalina, and A. E. Hendrickson, “Human photoreceptor topography,” J. Comparitive Neurology 292, 497–523 (1990).
[Crossref]

Hermann, B.

Hitzenberger, C. K.

Hofer, H.

Hong, X.

L. N. Thibos, X. Hong, A. Bradley, and R. A. Applegate, “Accuracy and precision of methods to predict the results of subjective refraction from monochromatic wavefront aberration maps,” J. Vis. 4, 329–351 (2004).
[PubMed]

L. N. Thibos, X. Hong, A. Bradley, and X. Cheng, “Statistical variation of aberration structure and image quality in a normal population of healthy eyes,” J. Opt. Soc. Am. A 19, 2329–2348 (2002).
[Crossref]

Hougaard, J. L.

B. Sander, M Larsen, L. Thrane, J. L. Hougaard, and T. M. Jørgensen, “Enhanced optical coherence tomography imaging by multiple scan averaging,” Br. J. Ophthalmol. 89, 207–212 (2004).
[Crossref]

Hu, Y.

N. Ling, Y. Zhang, X. Rao, X. Li, C. Wang, Y. Hu, and W. Jiang, “Small table-top adaptive optical systems for human retinal imaging”, in High-Resolution Wavefront Control: Methods, Devices, and Applications IV, J. D. Gonglewski, M. A. Vorontsov, M. T. Gruneisen, S. R. Restaino, and R. K. Tyson, eds., Proc. SPIE4825, 99–108 (2002).

Huang, D.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, “Optical coherence tomography,” Science 254, 1178–1181 (1991).
[Crossref] [PubMed]

Izatt, J. A.

R. J. Zawadzki, B. A. Bower, M. Zhao, M. Sarunic, S. Laut, J. S. Werner, and J. A. Izatt, “Exposure time dependence of image quality in high-speed retinal in vivo Fourier domain OCT,” in Ophthalmic Technologies XV, F. Manns, P. G. Soederberg, A. Ho, B. E. Stuck, and M. Belkin, eds., Proc. SPIE5688, 45–52 (2005).

J.C.,

Jiang, W.

W. Jiang and H. Li, “Hartmann-Shack wavefront sensing and control algorithm,” in Adaptive Optics and Optical Structures, J. J. Schulte-in-den-Baeumen and R. K. Tyson, eds., Proc. SPIE1271, 82–93 (1990).

N. Ling, Y. Zhang, X. Rao, X. Li, C. Wang, Y. Hu, and W. Jiang, “Small table-top adaptive optical systems for human retinal imaging”, in High-Resolution Wavefront Control: Methods, Devices, and Applications IV, J. D. Gonglewski, M. A. Vorontsov, M. T. Gruneisen, S. R. Restaino, and R. K. Tyson, eds., Proc. SPIE4825, 99–108 (2002).

Jonnal, R. J.

K. E. Thorn, J. Qu, R. J. Jonnal, and D. T. Miller, “Adaptive optics flood-illuminated camera for high speed retinal imaging,” Invest. Ophthalmol. Vis. Sci. 44, E-Abstract 1002 (2003).

Jonnal, R. S.

Y. Zhang, J. Rha, R. S. Jonnal, and D. T. Miller, “Single shot retinal imaging with AO spectral OCT,” in Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine IX, V. V. Tuchin, J. A. Izatt, and J. G. Fujimoto, eds. Proc. SPIE5690, 548–555 (2005).

D. T. Miller, J. Qu, R. S. Jonnal, and K. Thorn , “Coherence gating and adaptive optics in the eye”, in Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VII, V. V. Tuchin, J. A. Izatt, and J. G. Fujimoto, eds., Proc. SPIE4956, 65–72 (2003).

Jørgensen, T. M.

B. Sander, M Larsen, L. Thrane, J. L. Hougaard, and T. M. Jørgensen, “Enhanced optical coherence tomography imaging by multiple scan averaging,” Br. J. Ophthalmol. 89, 207–212 (2004).
[Crossref]

Kalina, R. E.

C. A. Curcio, K. R. Sloan, R. E. Kalina, and A. E. Hendrickson, “Human photoreceptor topography,” J. Comparitive Neurology 292, 497–523 (1990).
[Crossref]

Kärtner, F. X.

W. Drexler, U. Morgner, R. K. Ghanta, F. X. Kärtner, J. S. Schuman, and J. G. Fujimoto, “Ultrahigh-resolution ophthalmic optical coherence tomography,” Nature Med. 7, 502–507 (2001).
[Crossref] [PubMed]

Ko, T. H.

Kowalczyk, A.

Lacombe, F.

M. Glanc, E. Gendron, F. Lacombe, D. Lafaille, J.-F. Le Gargasson, and P. Léna, “Towards wide-field retinal imaging with adaptive optics,” Opt. Comm. 230, 225–238 (2004).
[Crossref]

Lafaille, D.

M. Glanc, E. Gendron, F. Lacombe, D. Lafaille, J.-F. Le Gargasson, and P. Léna, “Towards wide-field retinal imaging with adaptive optics,” Opt. Comm. 230, 225–238 (2004).
[Crossref]

Larsen, M

B. Sander, M Larsen, L. Thrane, J. L. Hougaard, and T. M. Jørgensen, “Enhanced optical coherence tomography imaging by multiple scan averaging,” Br. J. Ophthalmol. 89, 207–212 (2004).
[Crossref]

Laut, S.

R. J. Zawadzki, B. A. Bower, M. Zhao, M. Sarunic, S. Laut, J. S. Werner, and J. A. Izatt, “Exposure time dependence of image quality in high-speed retinal in vivo Fourier domain OCT,” in Ophthalmic Technologies XV, F. Manns, P. G. Soederberg, A. Ho, B. E. Stuck, and M. Belkin, eds., Proc. SPIE5688, 45–52 (2005).

Le, T.

Le Gargasson, J.-F.

M. Glanc, E. Gendron, F. Lacombe, D. Lafaille, J.-F. Le Gargasson, and P. Léna, “Towards wide-field retinal imaging with adaptive optics,” Opt. Comm. 230, 225–238 (2004).
[Crossref]

Leitgeb, R.

Leitgeb, R. A.

Léna, P.

M. Glanc, E. Gendron, F. Lacombe, D. Lafaille, J.-F. Le Gargasson, and P. Léna, “Towards wide-field retinal imaging with adaptive optics,” Opt. Comm. 230, 225–238 (2004).
[Crossref]

Li, H.

W. Jiang and H. Li, “Hartmann-Shack wavefront sensing and control algorithm,” in Adaptive Optics and Optical Structures, J. J. Schulte-in-den-Baeumen and R. K. Tyson, eds., Proc. SPIE1271, 82–93 (1990).

Li, X.

N. Ling, Y. Zhang, X. Rao, X. Li, C. Wang, Y. Hu, and W. Jiang, “Small table-top adaptive optical systems for human retinal imaging”, in High-Resolution Wavefront Control: Methods, Devices, and Applications IV, J. D. Gonglewski, M. A. Vorontsov, M. T. Gruneisen, S. R. Restaino, and R. K. Tyson, eds., Proc. SPIE4825, 99–108 (2002).

Liang, J.

Lin, C. P.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, “Optical coherence tomography,” Science 254, 1178–1181 (1991).
[Crossref] [PubMed]

Ling, N.

N. Ling, Y. Zhang, X. Rao, X. Li, C. Wang, Y. Hu, and W. Jiang, “Small table-top adaptive optical systems for human retinal imaging”, in High-Resolution Wavefront Control: Methods, Devices, and Applications IV, J. D. Gonglewski, M. A. Vorontsov, M. T. Gruneisen, S. R. Restaino, and R. K. Tyson, eds., Proc. SPIE4825, 99–108 (2002).

Malacara, D.

D. Malacara, Optical Shop Testing2nd ed. (John Wiley & Sons, New York, 1992), 112–113.

Miller, D. T.

K. E. Thorn, J. Qu, R. J. Jonnal, and D. T. Miller, “Adaptive optics flood-illuminated camera for high speed retinal imaging,” Invest. Ophthalmol. Vis. Sci. 44, E-Abstract 1002 (2003).

J. Liang, D. R. Williams, and D. T. Miller, “Supernormal vision and high resolution retinal imaging through adaptive optics,” J. Opt. Soc. Am. A 14, 2884–2892 (1997).
[Crossref]

D. T. Miller, D. R. Williams, G. M. Morris, and J. Liang, “Images of cone photoreceptors in the living human eye,” Vision Res. 36, 1067–1079 (1996).
[Crossref] [PubMed]

Y. Zhang, J. Rha, R. S. Jonnal, and D. T. Miller, “Single shot retinal imaging with AO spectral OCT,” in Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine IX, V. V. Tuchin, J. A. Izatt, and J. G. Fujimoto, eds. Proc. SPIE5690, 548–555 (2005).

D. T. Miller, J. Qu, R. S. Jonnal, and K. Thorn , “Coherence gating and adaptive optics in the eye”, in Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VII, V. V. Tuchin, J. A. Izatt, and J. G. Fujimoto, eds., Proc. SPIE4956, 65–72 (2003).

Morgner, U.

W. Drexler, U. Morgner, R. K. Ghanta, F. X. Kärtner, J. S. Schuman, and J. G. Fujimoto, “Ultrahigh-resolution ophthalmic optical coherence tomography,” Nature Med. 7, 502–507 (2001).
[Crossref] [PubMed]

Morris, G. M.

D. T. Miller, D. R. Williams, G. M. Morris, and J. Liang, “Images of cone photoreceptors in the living human eye,” Vision Res. 36, 1067–1079 (1996).
[Crossref] [PubMed]

Nassif, N.

Nassif, N. A.

Park, B. H.

Pierce, M. C.

Pircher, M.

Prieto, P. M.

Puliafito, C. A.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, “Optical coherence tomography,” Science 254, 1178–1181 (1991).
[Crossref] [PubMed]

Qu, J.

K. E. Thorn, J. Qu, R. J. Jonnal, and D. T. Miller, “Adaptive optics flood-illuminated camera for high speed retinal imaging,” Invest. Ophthalmol. Vis. Sci. 44, E-Abstract 1002 (2003).

D. T. Miller, J. Qu, R. S. Jonnal, and K. Thorn , “Coherence gating and adaptive optics in the eye”, in Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VII, V. V. Tuchin, J. A. Izatt, and J. G. Fujimoto, eds., Proc. SPIE4956, 65–72 (2003).

Queener, H.

Rao, X.

N. Ling, Y. Zhang, X. Rao, X. Li, C. Wang, Y. Hu, and W. Jiang, “Small table-top adaptive optical systems for human retinal imaging”, in High-Resolution Wavefront Control: Methods, Devices, and Applications IV, J. D. Gonglewski, M. A. Vorontsov, M. T. Gruneisen, S. R. Restaino, and R. K. Tyson, eds., Proc. SPIE4825, 99–108 (2002).

Rha, J.

Y. Zhang, J. Rha, R. S. Jonnal, and D. T. Miller, “Single shot retinal imaging with AO spectral OCT,” in Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine IX, V. V. Tuchin, J. A. Izatt, and J. G. Fujimoto, eds. Proc. SPIE5690, 548–555 (2005).

Riggs, L. A.

Romero-Borja, F.

Roorda, A.

Sander, B.

B. Sander, M Larsen, L. Thrane, J. L. Hougaard, and T. M. Jørgensen, “Enhanced optical coherence tomography imaging by multiple scan averaging,” Br. J. Ophthalmol. 89, 207–212 (2004).
[Crossref]

Sarunic, M.

R. J. Zawadzki, B. A. Bower, M. Zhao, M. Sarunic, S. Laut, J. S. Werner, and J. A. Izatt, “Exposure time dependence of image quality in high-speed retinal in vivo Fourier domain OCT,” in Ophthalmic Technologies XV, F. Manns, P. G. Soederberg, A. Ho, B. E. Stuck, and M. Belkin, eds., Proc. SPIE5688, 45–52 (2005).

Sattmann, H.

Schuman, J. S.

W. Drexler, U. Morgner, R. K. Ghanta, F. X. Kärtner, J. S. Schuman, and J. G. Fujimoto, “Ultrahigh-resolution ophthalmic optical coherence tomography,” Nature Med. 7, 502–507 (2001).
[Crossref] [PubMed]

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, “Optical coherence tomography,” Science 254, 1178–1181 (1991).
[Crossref] [PubMed]

Singer, B.

Sloan, K. R.

C. A. Curcio, K. R. Sloan, R. E. Kalina, and A. E. Hendrickson, “Human photoreceptor topography,” J. Comparitive Neurology 292, 497–523 (1990).
[Crossref]

Srinivasan, V. J.

Stingl, A.

Stinson, W. G.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, “Optical coherence tomography,” Science 254, 1178–1181 (1991).
[Crossref] [PubMed]

Swanson, E. A.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, “Optical coherence tomography,” Science 254, 1178–1181 (1991).
[Crossref] [PubMed]

Tearney, G. J.

Thibos, L. N.

L. N. Thibos, X. Hong, A. Bradley, and R. A. Applegate, “Accuracy and precision of methods to predict the results of subjective refraction from monochromatic wavefront aberration maps,” J. Vis. 4, 329–351 (2004).
[PubMed]

L. N. Thibos, X. Hong, A. Bradley, and X. Cheng, “Statistical variation of aberration structure and image quality in a normal population of healthy eyes,” J. Opt. Soc. Am. A 19, 2329–2348 (2002).
[Crossref]

Thorn, K.

D. T. Miller, J. Qu, R. S. Jonnal, and K. Thorn , “Coherence gating and adaptive optics in the eye”, in Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VII, V. V. Tuchin, J. A. Izatt, and J. G. Fujimoto, eds., Proc. SPIE4956, 65–72 (2003).

Thorn, K. E.

K. E. Thorn, J. Qu, R. J. Jonnal, and D. T. Miller, “Adaptive optics flood-illuminated camera for high speed retinal imaging,” Invest. Ophthalmol. Vis. Sci. 44, E-Abstract 1002 (2003).

Thrane, L.

B. Sander, M Larsen, L. Thrane, J. L. Hougaard, and T. M. Jørgensen, “Enhanced optical coherence tomography imaging by multiple scan averaging,” Br. J. Ophthalmol. 89, 207–212 (2004).
[Crossref]

Tyson, R. K.

R. K. Tyson, Principles of Adaptive Optics (Academic Press, New York, 1998).

Unterhuber, A.

Wang, C.

N. Ling, Y. Zhang, X. Rao, X. Li, C. Wang, Y. Hu, and W. Jiang, “Small table-top adaptive optical systems for human retinal imaging”, in High-Resolution Wavefront Control: Methods, Devices, and Applications IV, J. D. Gonglewski, M. A. Vorontsov, M. T. Gruneisen, S. R. Restaino, and R. K. Tyson, eds., Proc. SPIE4825, 99–108 (2002).

Werner, J. S.

R. J. Zawadzki, B. A. Bower, M. Zhao, M. Sarunic, S. Laut, J. S. Werner, and J. A. Izatt, “Exposure time dependence of image quality in high-speed retinal in vivo Fourier domain OCT,” in Ophthalmic Technologies XV, F. Manns, P. G. Soederberg, A. Ho, B. E. Stuck, and M. Belkin, eds., Proc. SPIE5688, 45–52 (2005).

Williams, D. R.

Wojtkowski, M.

Yamauchi, Y.

Yoon, G. Y.

Yun, S.

Yun, S. H.

Zawadzki, R. J.

R. J. Zawadzki, B. A. Bower, M. Zhao, M. Sarunic, S. Laut, J. S. Werner, and J. A. Izatt, “Exposure time dependence of image quality in high-speed retinal in vivo Fourier domain OCT,” in Ophthalmic Technologies XV, F. Manns, P. G. Soederberg, A. Ho, B. E. Stuck, and M. Belkin, eds., Proc. SPIE5688, 45–52 (2005).

Zhang, Y.

Y. Zhang, J. Rha, R. S. Jonnal, and D. T. Miller, “Single shot retinal imaging with AO spectral OCT,” in Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine IX, V. V. Tuchin, J. A. Izatt, and J. G. Fujimoto, eds. Proc. SPIE5690, 548–555 (2005).

N. Ling, Y. Zhang, X. Rao, X. Li, C. Wang, Y. Hu, and W. Jiang, “Small table-top adaptive optical systems for human retinal imaging”, in High-Resolution Wavefront Control: Methods, Devices, and Applications IV, J. D. Gonglewski, M. A. Vorontsov, M. T. Gruneisen, S. R. Restaino, and R. K. Tyson, eds., Proc. SPIE4825, 99–108 (2002).

Zhao, M.

R. J. Zawadzki, B. A. Bower, M. Zhao, M. Sarunic, S. Laut, J. S. Werner, and J. A. Izatt, “Exposure time dependence of image quality in high-speed retinal in vivo Fourier domain OCT,” in Ophthalmic Technologies XV, F. Manns, P. G. Soederberg, A. Ho, B. E. Stuck, and M. Belkin, eds., Proc. SPIE5688, 45–52 (2005).

Br. J. Ophthalmol. (1)

B. Sander, M Larsen, L. Thrane, J. L. Hougaard, and T. M. Jørgensen, “Enhanced optical coherence tomography imaging by multiple scan averaging,” Br. J. Ophthalmol. 89, 207–212 (2004).
[Crossref]

Invest. Ophthalmol. Vis. Sci. (1)

K. E. Thorn, J. Qu, R. J. Jonnal, and D. T. Miller, “Adaptive optics flood-illuminated camera for high speed retinal imaging,” Invest. Ophthalmol. Vis. Sci. 44, E-Abstract 1002 (2003).

J. Comparitive Neurology (1)

C. A. Curcio, K. R. Sloan, R. E. Kalina, and A. E. Hendrickson, “Human photoreceptor topography,” J. Comparitive Neurology 292, 497–523 (1990).
[Crossref]

J. Opt. Soc. Am. (1)

J. Opt. Soc. Am. A (3)

J. Vis. (1)

L. N. Thibos, X. Hong, A. Bradley, and R. A. Applegate, “Accuracy and precision of methods to predict the results of subjective refraction from monochromatic wavefront aberration maps,” J. Vis. 4, 329–351 (2004).
[PubMed]

Nature Med. (1)

W. Drexler, U. Morgner, R. K. Ghanta, F. X. Kärtner, J. S. Schuman, and J. G. Fujimoto, “Ultrahigh-resolution ophthalmic optical coherence tomography,” Nature Med. 7, 502–507 (2001).
[Crossref] [PubMed]

Opt. Comm. (1)

M. Glanc, E. Gendron, F. Lacombe, D. Lafaille, J.-F. Le Gargasson, and P. Léna, “Towards wide-field retinal imaging with adaptive optics,” Opt. Comm. 230, 225–238 (2004).
[Crossref]

Opt. Express (7)

B. Grajciar, M. Pircher, A. F. Fercher, and R. A. Leitgeb, “Parallel Fourier domain optical coherence tomography for in vivo measurement of the human eye,” Opt. Express 13, 1131–1137 (2005), http://www.opticsexpress.org/abstract.cfm?URI=OPEX-13-4-1131.
[Crossref] [PubMed]

B. Cense, N. A. Nassif, T. C. Chen, M. C. Pierce, S. Yun, B. H. Park, B. E. Bouma, G. J. Tearney, and J. F. de Boer, “Ultrahigh-resolution high-speed retinal imaging using spectral-domain optical coherence tomography,” Opt. Express 12, 2435–2447 (2004), http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-11-2435.
[Crossref] [PubMed]

M. Wojtkowski, V. J. Srinivasan, T. H. Ko, J. G. Fujimoto, A. Kowalczyk, and J. S. Duker, “Ultrahigh-resolution, high-speed, Fourier domain optical coherence tomography and methods for dispersion compensation,” Opt. Express 12, 2404–2421 (2004), http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-11-2404.
[Crossref] [PubMed]

R. A. Leitgeb, W. Drexler, A. Unterhuber, B. Hermann, T. Bajraszewski, T. Le, A. Stingl, and A. F. Fercher, “Ultrahigh resolution Fourier domain optical coherence tomography”, Opt. Express 12, 2156–2165 (2004), http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-10-2156.
[Crossref] [PubMed]

H. Hofer, L. Chen, G. Y. Yoon, B. Singer, Y. Yamauchi, and D. R. Williams, “Improvement in retinal image quality with dynamic correction of the eye’s aberrations,” Opt. Express 8, 631–643 (2001), http://www.opticsexpress.org/abstract.cfm?URI=OPEX-8-11-631.
[Crossref] [PubMed]

A. Roorda, F. Romero-Borja, W. J. Donnelly, H. Queener, T. J. Hebert, and M. C. W. Campbell, “Adaptive optics scanning laser ophthalmoscopy,” Opt. Express 10, 405–412 (2002), http://www.opticsexpress.org/abstract.cfm?URI=OPEX-10-9-405.
[PubMed]

R. Leitgeb, C. K. Hitzenberger, and A. F. Fercher, “Performance of Fourier domain versus time domain optical coherence tomography,” Opt. Express 11, 889–894 (2003), http://www.opticsexpress.org/abstract.cfm?URI=OPEX-11-8-889.
[Crossref] [PubMed]

Opt. Lett. (2)

Science (1)

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, “Optical coherence tomography,” Science 254, 1178–1181 (1991).
[Crossref] [PubMed]

Vision Res. (2)

D. R. Williams, “Topography of the foveal cone mosaic in the living human eye,” Vision Res. 28, 433–454 (1988).
[Crossref] [PubMed]

D. T. Miller, D. R. Williams, G. M. Morris, and J. Liang, “Images of cone photoreceptors in the living human eye,” Vision Res. 36, 1067–1079 (1996).
[Crossref] [PubMed]

Other (9)

R. J. Zawadzki, B. A. Bower, M. Zhao, M. Sarunic, S. Laut, J. S. Werner, and J. A. Izatt, “Exposure time dependence of image quality in high-speed retinal in vivo Fourier domain OCT,” in Ophthalmic Technologies XV, F. Manns, P. G. Soederberg, A. Ho, B. E. Stuck, and M. Belkin, eds., Proc. SPIE5688, 45–52 (2005).

D. Malacara, Optical Shop Testing2nd ed. (John Wiley & Sons, New York, 1992), 112–113.

R. K. Tyson, Principles of Adaptive Optics (Academic Press, New York, 1998).

Y. Zhang, J. Rha, R. S. Jonnal, and D. T. Miller, “Single shot retinal imaging with AO spectral OCT,” in Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine IX, V. V. Tuchin, J. A. Izatt, and J. G. Fujimoto, eds. Proc. SPIE5690, 548–555 (2005).

ANSI, American National Standard for the Safe Use of Lasers, ANSI Z136.1 (Laser Institute of America, Orlando, FL, 2000).

J. Hecht, Understanding Fiber Optics (Prentice Hall, New Jersey, 1998).

W. Jiang and H. Li, “Hartmann-Shack wavefront sensing and control algorithm,” in Adaptive Optics and Optical Structures, J. J. Schulte-in-den-Baeumen and R. K. Tyson, eds., Proc. SPIE1271, 82–93 (1990).

D. T. Miller, J. Qu, R. S. Jonnal, and K. Thorn , “Coherence gating and adaptive optics in the eye”, in Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VII, V. V. Tuchin, J. A. Izatt, and J. G. Fujimoto, eds., Proc. SPIE4956, 65–72 (2003).

N. Ling, Y. Zhang, X. Rao, X. Li, C. Wang, Y. Hu, and W. Jiang, “Small table-top adaptive optical systems for human retinal imaging”, in High-Resolution Wavefront Control: Methods, Devices, and Applications IV, J. D. Gonglewski, M. A. Vorontsov, M. T. Gruneisen, S. R. Restaino, and R. K. Tyson, eds., Proc. SPIE4825, 99–108 (2002).

Supplementary Material (1)

» Media 1: AVI (1732 KB)     

Cited By

OSA participates in Crossref's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (11)

Fig. 1.
Fig. 1.

(A) Concept layout shows the AO system as part of the SD-OCT detection channel. (B) Detailed layout of the AO parallel SD-OCT retina camera. The camera consists of four sub-systems. (1) AO system corrects the ocular aberrations using a 788 nm SLD, Shack-Hartmann wavefront sensor, and Xinetics deformable mirror (short dashed line). (2) Pupil retro-illumination and fixation channels permit alignment of the subject’s eye to the retina camera. (3) Conventional flood-illumination is used to validate focusing in the retina and the physical size of microstructures in the retina (solid line). (4) Parallel SD-OCT acquires single shot B-scan images of the retina (long dashed line). Further details of the camera are given in the text.

Download Full Size | PPT Slide | PDF

Fig. 2.
Fig. 2.

Parallel SD-OCT sensitivity as a function of depth, converted from depth measured in air to tissue by using a refractive index of n=1.38. The four A-scan locations are along the line illumination profile. A-scan location is in microns at the retina, e.g. the 25th A-scan is 25 µm from the central A-scan.

Download Full Size | PPT Slide | PDF

Fig. 3.
Fig. 3.

Total wavefront error over time as measured by the SHWS before and during dynamic correction on one subject. The two traces depict correction with and without the middle five actuators frozen to a fixed correction (the remaining 32 actuators continued to provide dynamic correction). The time at which the actuators are frozen is indicated with no appreciable difference in the traces observed afterward.

Download Full Size | PPT Slide | PDF

Fig. 4.
Fig. 4.

Raw single cone images collected on the subject with the conventional flood-illuminated sub-system as depicted in Fig. 1. The four images represent the sharpest collected at each of two retinal eccentricities (1 and 2.4 deg) with and without AO. Images are 0.67×0.33 deg (200×100 µm) in size and were collected using the 843 nm SLD. Exposure duration was 4 msec, and imaging pupil size was 6 mm.

Download Full Size | PPT Slide | PDF

Fig. 5.
Fig. 5.

(top) Stratus OCT3 and (bottom) scanning SD-OCT B-scans collected in the same subject. Images are centered on the fovea and bisect the superior and inferior retinal fields (Stratus OCT3), and nasal and temporal fields (scanning SD-OCT). B-scans are 4.9 mm (16.3 deg) wide and 0.75 mm in depth. White rectangles depict 100 µm wide by 560 µm deep subsections that are centered at 1 and 2.4 deg eccentricity and imaged with the AO parallel SD-OCT camera. A magnified view of these subsections is shown in Fig. 6 with the corresponding AO parallel SD-OCT B-scans.

Download Full Size | PPT Slide | PDF

Fig. 6.
Fig. 6.

(left two columns) B-scan images acquired with the AO parallel SD-OCT instrument in Fig. 2 with and without AO at 1 and 2.4 deg eccentricity (superior). (right two columns) Stratus OCT3 and scanning SD-OCT B-scans are shown at the same retinal eccentricities (from white rectangular boxes in Fig. 5). All images were acquired on the same subject and are 100 µm wide and 560 µm deep. (bottom) The interface between the inner and outer segments and RPE are enlarged and displayed as amplitude on a linear scale (as opposed to a log scale). Minor thresholding was used to enhance contrast. (far right) Labels for retinal layers are repeated from Fig. 5 and positioned where they should appear in the scanning SD-OCT image. Images without AO are normalized to the corresponding AO images, including the enlarged images. The apparent tilt in the Stratus OCT3 image at 2.4 deg is an eye motion artifact. The Stratus OCT3 images were acquired at 9.6 µm/A-scan and interpolated (bicubic) to 1 µm. The scanning SD-OCT images were acquired at 2 µm/A-scan and interpolated (linear) to 1 µm. The 2 and 9.6 µm sampling intervals are noticeably smaller than their respective lateral resolutions. Depth of focus (dof) is displayed at the left and defined in the text.

Download Full Size | PPT Slide | PDF

Fig. 7.
Fig. 7.

Two collages created by digitally pasting together an alternating sequence of AO parallel SD-OCT images acquired at 1 and 2.4 deg eccentricity. The collages are roughly 3.25 to 3.5 degrees wide. Focus is at the cones. Each set of images was taken from the same short burst videos. The collage at 2.4 deg eccentricity was generated from two images (each 70 A-scans from the central region of a B-scan; dashed white rectangle represents the combined two images and is 140 A-scans) that were repeated about 7 times. The collage at 1 deg eccentricity contains three images (each ~70 A-scans; dashed white rectangle represents 210 A-scans) that were repeated about 5 times. The collages were axially registered to each other by aligning the reflection from the IS/OS segment. Note the enhanced clarity of the major retinal layers compared to that for the highly magnified and narrow AO parallel SD-OCT images in Fig. 6.

Download Full Size | PPT Slide | PDF

Fig. 8.
Fig. 8.

Five-burst B-scan videos collected of the same patch of retina at 2.4 deg eccentricity for three scenarios: (left) focused on the cones and without AO, (middle) focused on the cones and with AO; (right) focus shifted anteriorly 200 µm and with AO. Note that the physical separation between the ILM (upper edge of the topmost bright layer) and anterior side of the photoreceptor outer segments (segmented bright line in lower half) is around 272 µm. Each B-scan is composed of 100 A-scans spaced 1 µm apart. Videos were captured at 500 Hz with 1 msec exposure and run at 4 Hz, which is 125 times slower than the actual acquisition rate. The videos are best viewed in “loop” mode.

Download Full Size | PPT Slide | PDF

Fig. 9.
Fig. 9.

Lateral reflectance profiles through the elongated structures (photoreceptor outer segments) for AO-corrected B-scan images at 2.4 (red) and 1 (blue) deg eccentricities for two short-burst sequences. Frame numbers correspond to the temporal sequence within a single short-burst series where frames 1, 5, and 7 are separated by 8 and 4 msecs, respectively. Profiles are shifted vertically in the plot to facilitate visual comparison. Black arrows indicate the centers of cones whose profiles are stable between frames and insensitive to small retinal motion perpendicular to the slit. Gray arrows indicate the edges of cones and reveal the apparent separation or merging of adjacent cones due to retinal motion perpendicular to the slit. Retinal motion parallel to the slit causes a shift in the entire profile.

Download Full Size | PPT Slide | PDF

Fig. 10.
Fig. 10.

Average power spectra obtained by 1D Fourier transformation (black dashed curves) of the conventional flood illuminated AO images in Fig. 4 after being sampled by the 2.8×100 µm slit and (red and blue curves) of cross sectional slices through the interface between the inner and outer segments, outer plexiform layer, outer nuclear layer, and inner plexiform layer. (left) Power spectra are shown for different depths in the retina at the 2.4 deg eccentricity. (center and right) Power spectra are shown with and without AO at eccentricities of 2.4 and 1 degrees, respectively. All parallel SD-OCT curves are normalized to have the same power at 0 cyc/deg.

Download Full Size | PPT Slide | PDF

Fig. 11.
Fig. 11.

Average profile of 20 contiguous A-scans centered about the brightest region in the B-scan for each of the three imaging scenarios shown in Fig. 8. Images were obtained at 2.4 deg retinal eccentricity.

Download Full Size | PPT Slide | PDF

Tables (2)

Tables Icon

Table 1. Measured wavefront aberrations across a 6.8 mm pupil for one subject with and without AO compensation. RMS wavefront error is shown for the total aberrations, Zernike defocus and astigmatism, and combined 3rd order and higher.

View Table | View all tables in this article
Tables Icon

Table 2. Average SNR for cones and NFL for three imaging scenarios as described in the text.

View Table | View all tables in this article

Metrics