Abstract

We report on a new method based on speckle size analysis and devoted to particle aggregation measurements. The experimental measurements give the speckle size variation during a salt aggregation process of polystyrene microspheres. The measurements are taken at a fixed monomer concentration, varying the salt concentration. Moreover, we applied this technique to follow blood platelet aggregation, usually monitored with a visible light transmittance photometer (aggregometer). Aggregation process was induced by ADP (adenosine diphosphate) addition, then we measured the speckle size variation versus time at two different ADP concentrations.

© 2004 Optical Society of America

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References

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Appl. Opt.

J. Opt. Soc. Am. A

Opt. Express

Phys. Rev. A

J.P. Wilcoxon, J.E. Martin, D.W. Shaefer, �??Aggregation in colloidal gold,�?? Phys. Rev. A 39, 2675-2688 (1989).
[CrossRef] [PubMed]

M. Carpinetti, F. Ferri, M. Giglio, E. Paganini, U. Perini, �??Salt-induced fast aggregation of polystyrene latex,�?? Phys. Rev. A 42, 7347-7354 (1990).
[CrossRef]

D. Majolino, F. Mallamace, P. Migliardo, N. Micali, C. Vasi, �??Elastic and quasielastic light-scattering studies of the aggregation phenomena in water solutions of polystyrene particles,�?? Phys. Rev. A 40, 4665-4674 (1989).
[CrossRef] [PubMed]

Proc. Natl. Acad. Sci.

Y. Georgalis, E.B. Starikov, B. Hollenbach, R. Lurz, Eberhard Scherzinger, W. Sger, H. Lehrach, E. Wanker, �??Huntingtin aggregation monitored by dynamic light scattering,�?? Proc. Natl. Acad. Sci. 95, 6118-6121 (1998).
[CrossRef] [PubMed]

Sysmex J. Int.

Y. Ozaki, �??Measurement of platelet aggregation and attempts for standardization,�?? Sysmex J. Int. 8, 15-22 (1998).

Other

H.C. Van de Hulst, Light scattering by small particles (New York, Dover, 1981).

J.W. Goodman, �??Statistical Properties of Laser Speckle Patterns,�?? in Laser speckle and related phenomena, Vol. 9 in series Topics in Applied Physics, J.C. Dainty, Ed., (Springer-Verlag, Berlin, Heidelberg New York Tokyo, 1984).

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Figures (3)

Fig. 1.
Fig. 1.

Experimental set-up (top view).

Fig.2.
Fig.2.

dy versus t during aggregation process at several salt concentrations with the respective linear fitting curves:

Fig.3.
Fig.3.

dy versus the time t during platelet aggregation process obtained for two ADP concentration

Equations (5)

Equations on this page are rendered with MathJax. Learn more.

R I ( Δ x , Δ y ) = I x 1 y 1 I x 2 y 2
R I ( Δ x , Δ y ) = R I x y
c I x y = R I x y I x y 2 I x y 2 I x y 2
PS D I υ x υ y = FT [ I x y ] 2
c I x y = F T 1 [ FT [ I x y ] 2 ] I x y 2 I x y 2 I x y 2

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