Abstract

Mid-infrared (MIR) (3-12 um) spectral imaging is a power analytical tool, but difficult in the back-reflectance mode for in-vivo diagnostics. Feasibility of MIR back-reflectance imaging is demonstrated using MIR semiconductor lasers. Transmittance through 500-µm thick films of water and blood showed a capability to resolve more than 6-OD signal dynamic range. Reflectance scanning imaging through a 150-µm thick film of blood showed negligible scattering effect, indicating the feasibility of optical coherent imaging. The result of coherent imaging of a plant leaf shows a MIR sub-surface image that would not be visible in white light. With two wavelengths, a similar result for a chicken skin subcutaneous tissue at different focal depths was obtained, showing blood vessels beneath a lipid layer. These results suggest that advanced multilaser wavelength systems in the fingerprint spectral region can be a useful tool for in-vivo spectral imaging in biomedical research and diagnostic applications.

© 2004 Optical Society of America

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Adv. Exp. Med. Biol.

Y. Chen, X. Intes, D.R. Tailor, R.R. Regatte, H. Ma, V. Ntziachristos, J.S. Leigh, R. Reddy, B. Chance, �??Probing rat brain oxygenation with near-infrared spectroscopy (NIRS) and magnetic resonance imaging (MRI),�?? Adv. Exp. Med. Biol. 510, 199-204, (2003).

Analytical Chemistry

J. L. Koenig, S-Q. Wang, and R. Bhargava, �??FTIR Images,�?? Analytical Chemistry July 1, 360A (2001).

Appl. Opt.

Appl. Spectrosc.

Appl. Spectrosc. Rev.

P. J. Treado and M. D. Morris, �??Infrared and Raman Spectroscopic Imaging,�?? Appl. Spectrosc. Rev. 29, 1 (1994).

Burns

M.G. Sowa, L. Leonardi, J.R. Payette, J.S. Fish, and H.H. Mantsch, �??Near infrared spectroscopic assessment of hemodynamic changes in the early post-burn period,�?? Burns 27, 241-249 (2001).
[CrossRef]

IEEE J. Quantum Electron.

G. Luo, C, Peng, H.-Q. Le, S.-S. Pei, H. Lee, W.-Y. Hwang, B. and J. Zheng, �??Broadly Wavelength-Tunable External Cavity Mid-Infrared Quantum Cascade Lasers,�?? IEEE J. Quantum Electron. 38, 931 (2002).

J. Am. Coll. Cardiol.

J. Wang, Y.J. Geng, B. Guo, T. Klima, B.N. Lal, J.T. Willerson, W. Casscells, �??Near-infrared spectroscopic characterization of human advanced atherosclerotic plaques,�?? J. Am. Coll. Cardiol. 39, 1305 (2002).
[CrossRef]

J. Interv. Cardiol.

P.R. Moreno, J.E. Muller, �??Detection of high-risk atherosclerotic coronary plaques by intravascular spectroscopy,�?? J. Interv. Cardiol. 16, 243 (2003).
[CrossRef]

Microsc. Microanal.

R. A. Crocombe, N. Wright, D. L. Drapcho, W. J. McCarthy, P. Bhandare, E. Y. Jiang, �??FT-IR spectroscopic imaging in the infrared 'fingerprint' region using an MCT array detector,�?? Microsc. Microanal. 3, Supplement 2, 863�??864 (1997).

Phys. Med. Biol.

A.L. McKenzie, �??Physics of Thermal Processes in Laser-Tissue Interaction,�?? Phys. Med. Biol. 35, 1175-1209 (1990).
[CrossRef]

Proc. Natl. Acad. Sci. USA

N.G. Jamin, P. Dumas, J. Moncuit, W.H. Fridman, J.L. Teillaud, G.L. Carr, and G.P. Williams, �??Highly resolved chemical imaging of living cells by using synchrotron infrared microspectrometry,�?? Proc. Natl. Acad. Sci. USA 95, 4837�??40, (1998).

Surgery and Medicine

S.L. Jacques, J.R. Roman, and K. Lee, �??Lasers in Imaging Superficial Tissues with Polarized Light,�?? Surgery and Medicine 26, 119�??129 (2000).

Other

W.K. Purves, G.H. Orians, H.C. Heller, and D. Sadava, Life, The Science of Biology, 5th Ed. (Sinauer Associates, W.H. Frreeman and Company, 1998).

J. Dingle, �??Manufacturing leather from chicken skin,�?? RIRDC Publication No 01/154, Project No. UQ-24E, (Rural Industries Research and Development Corporation, 2001).

P.R. Griffiths and J.M. Olinger, �??Continuum Theories of Diffuse Reflectance�?? in Handbook of Vibrational Spectroscopy, J.M. Chalmers and P.R. Griffiths, ed., pp 1125-1138, Volume II, (John Wiley and Sons, Ltd., UK, 2002).

D.J. Dahm and K.D. Dahm, �??Discontinuum Theory of Diffuse Reflectance,�?? in Handbook of Vibrational Spectroscopy, J.M. Chalmers and P.R. Griffiths, ed., pp1140-1153, Volume II, (John Wiley and Sons, Ltd., UK, 2002).

E. N. Lewis, I. W. Levin, P. J. Treado, U.S. Patent 5,528,368 (1996).

R.G. Messerschmidt, M.A. Harthcock, eds. Infrared Microspectroscopy: Theory and applications. (Marcel Dekker, New York, 1988).

H.H. Mantsch, and D. Chapman, eds. Infrared Spectroscopy of Biomolecules. (Wiley-Liss, New York, 1996).

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