Abstract

Pronounced separation (750 nm) between two individual fluorescence spots in a novel super-resolution microscopy based on a two-color up-conversion fluorescence depletion technique has been investigated. This microscopy has the potential to achieve a spatial resolution (<300nm) of 1/2 the diffraction limit.

© 2003 Optical Society of America

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References

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Appl. Spectrosc. (1)

IEEE J. Quntum Electronics (1)

E. Sahar and D. Treves, �??Excitation singlet-state absorption in dyes and their effect on dy lasers,�?? IEEE J. Quntum Electronics 13, 962-967 (1977)
[CrossRef]

J. Chem. Phys. (1)

T. Watanabe, Y. Iketaki, T. Omatsu, K. Yamamoto, S. Ishiuchi, M. Sakai and M. Fujii, �??Two color far-field super-resolution microscope using a doughnut beam,�?? J. Chem. Phys. 371, 634-639 (2003).

J. Phys. Chem. (1)

T. Ebata, N. Mizuochi, T. Watanabe and N. Mikami, �??OH stretching vibrations of Phenol-(H2O)1 and Phenol-(H2O)3 in the S1 State,�?? J. Phys. Chem. 100, 546-550 (1996).
[CrossRef]

Nature (2)

P. Davidvits and A.M.D. Egger, �??Scanning Laser Microscope,�?? Nature 48, 831, (1969).
[CrossRef]

E. H. K. Stelzer, �??Beyond the diffraction limit?�?? Nature, 417, 806-807 (2002).
[CrossRef] [PubMed]

Opt. Commun. (1)

G. Machavariani, N. Davidson, E. Hasman, S. Blit, A. A. Ishaaya and A. A. Friesem, �??Efficient conversion of a Gaussian beam to a high purity helical beam,�?? Opt. Commun. 209, 265-271 (2002)
[CrossRef]

Opt. Lett. (4)

Proc. SPIE (1)

Y. Kobayashi, Y. Igasaki, N. Yoshida, N. Fukuchi, H. Toyoda, T. Hara and M. H. Wu, �??Compact highefficiency electrically-addressable phase-only spatial light modulator,�?? in Diffractive/Holographic Technologies and Spatial Light Modulators VII, I. Cindrich, S. H. Lee and R. L. Sutherland, Proc. SPIE 3951, 158-165 (2000).
[CrossRef]

Science (1)

W. Denk, J. H. Strickler and W. W. Webb, �??Two-photon laser scanning fluorescence microscopy,�?? Science 248, 73-76 (1990).
[CrossRef] [PubMed]

Trends in Optics and Photonics (2)

Y. Iketaki, T. Omatsu, O. Sato, T. Suzuki and M. Fujii, �??Super-resolution fluorescence microscopy by upconversion-depletion using two color lasers,�?? in Trends in Optics and Photonics, vol. 39 of OSA Proceedings Series (Optical Society of America, Washington, D.C., 2000), pp.167-168.

T. Watanabe, Y. Iketaki, T. Omatsu, K. Yamamoto, S. Ishiuchi, M. Sakai and M. Fujii, �??Far-field super-resolution scanning fluorescence microscope using up-conversion-depletion technique,�?? in Trends in Optics and Photonics, OSA Proceedings Series (Optical Society of America, Washington, D.C., 2003), in press.

Other (3)

Fujii and M. Ito, Advances in Multiphoton Processes and Spectroscopy, Vol. 4, ed. by S. H. Lin (World Scientific, 1988).

T. Wilson and C. J. R. Sheppard, Theory and Practice of Scanning Optical Microscopy, (Academic, London, 1984).

P. Jaquinot and B. Roizen-Dossier, Progress in Optics, III, ed. by E. Wolf, (North-Holland, 1964)

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Figures (4)

Fig. 1.
Fig. 1.

(a) Excitation scheme of a two-color up-conversion fluorescence depletion technique. (b) Scheme for super-resolution microscopy based on a two-color up-conversion fluorescence depletion technique. The pump and “doughnut-shape” erase light are coaxially introduced to the sample.

Fig. 2.
Fig. 2.

Schematic diagram of the experimental setup for two-color super-resolution microscopy using a doughnut beam. PAL-SLM corresponds to a spatial light modulator.

Fig. 3.
Fig. 3.

Experimental scanning image of the ϕ175 nm micro-sphere obtained by (a) pump and “doughnut” erase beams, and (b) pump light only. The cross section is also shown below the images (c and d).

Fig. 4.
Fig. 4.

Experimental scanning image of micro-spheres obtained by (a) pump and “doughnut” erase beams, and (b) pump light only. The cross section profile along the red line is shown below the image (c and d).

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