Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
Journal Home About Issues in Progress Current Issue All Issues Feature Issues

Study of dynamic process of acetic acid induced-whitening in epithelial tissues at cellular level

Tao T. Wu, Jianan Y. Qu, Tak Hong Cheung, So Fan Yim, and Yick Fu Wong

Open Access Open Access

Abstract

Acetic acid, inducing transient whitening (acetowhitening) when applied to epithelial tissues, is a commonly used contrast agent for detecting early cervical cancer. The goals of this research are to investigate the temporal characteristics of acetowhitening process in cervical epithelial tissue at cellular level and develop a clear understanding of the diagnostic information carried in the acetowhitening signal. A system measuring time-resolved reflectance was built to study the rising and decay processes of acetowhitening signal from the monolayered cell cultures of normal and cancerous cervical squamous cells. It is found that the dynamic processes of acetowhitening in normal and cancerous cells are significantly different. The results of this study provide insight valuable to further understand the acetowhitening process in epithelial cells and to encourage the development of an objective procedure to detect the early cervical cancers based on quantitative monitoring of the dynamic process of acetowhitening.

©2005 Optical Society of America

Full Article  |  PDF Article
More Like This
Assessment of the relative contribution of cellular components to the acetowhitening effect in cell cultures and suspensions using elastic light-scattering spectroscopy

Tao T. Wu and Jianan Y. Qu
Appl. Opt. 46(21) 4834-4842 (2007)

Near Real Time Confocal Microscopy of Amelanotic Tissue: Dynamics of Aceto-Whitening Enable Nuclear Segmentation

Tom Collier, Peggy Shen, Benoit de Pradier, Kung-Bin Sung, Rebecca Richards-Kortum, Anais Malpica, and Michele Follen
Opt. Express 6(2) 40-48 (2000)

Optical imaging of cervical precancerous lesions based on active stereo vision and motion tracking

Tao T. Wu, Tak-Hong Cheung, So-Fan Yim, and Jianan Y. Qu
Opt. Express 16(15) 11224-11230 (2008)

View More...

Supplementary Material (3)

Media 1: MOV (1228 KB)     
Media 2: MOV (961 KB)     
Media 3: MOV (203 KB)     

Cited By

Optica participates in Crossref's Cited-By Linking service. Citing articles from Optica Publishing Group journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (8)
Fig. 1.
Fig. 1. Schematic of time-resolved reflectance measurement system. L1: Focus Lens (f=35mm); C1: Fiber Optic Collimator; A1: Aperture Stop; M1, M2: Mirrors.

Download Full Size | PDF

Fig. 2.
Fig. 2. Acetowhitening signals (right) and phase-contrast images (left) recorded from cancer cell samples in rising and decay courses. (a) (1.228MB) Concentration of acetic acid in medium: 0.3%; (b) (962KB) Concentration of acetic acid in medium: 1.2%.

Download Full Size | PDF

Fig. 3.
Fig. 3. Wavelength-dependence of acetowhitening signal. (a) RGB reflectance signals recorded from a cancer cell sample with the application of 0.6% acetic acid solution; (b) Comparison of RGB signals in the rising course; (c) Comparison of signals in the decay course.

Download Full Size | PDF

Fig. 4.
Fig. 4. Acetowhitening signals of cancer cell samples at different acetic acid concentrations. (a) (203KB) Movie of the acetowhitening signals as a function of acetic acid concentration; (b) The peak value of acetowhitening signals as a function of acetic acid concentration.

Download Full Size | PDF

Fig. 5.
Fig. 5. Comparison of the acetowhitening signals of cancer cell samples when applying the medium with different acetic acid concentrations. (a) Normalized signals in the rising courses; (b) Normalized signals in the decay courses.

Download Full Size | PDF

Fig. 6.
Fig. 6. Acetowhitening signals of normal cell samples at different acetic acid concentrations. (a) The acetowhitening signals as a function of acetic acid concentration; (b) The peak value of acetowhitening signals as a function of acetic acid concentration.

Download Full Size | PDF

Fig. 7.
Fig. 7. Comparison of single and dual-exponential models in fitting the decay of acetowhitening signals. (a) Cancer cell sample, 0.6% acetic acid; (b) Normal cell sample, 0.6% acetic acid.

Download Full Size | PDF

Fig. 8.
Fig. 8. (a) pH value and (b) H+ concentration (mol/L) of the acetic acid solution as a function of the concentration of acetic acid at room temperature 25°C.

Download Full Size | PDF

Tables (2)

Tables Icon

Table 1. The decay time constants for normal and cancer cell samples calculated from a single exponential model.

View Table | View all tables in this article
Tables Icon

Table 2. Analyses of decay process of acetowhitening effect in normal and cancer cells using dual-exponential model.

View Table | View all tables in this article
Select as filters
Select Topics Cancel
Top
       
© Copyright 2024 | Optica Publishing Group. All rights reserved, including rights for text and data mining and training of artificial technologies or similar technologies.
Privacy | Terms of Use