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Surface-enhanced resonance Raman scattering in optical tweezers using co-axial second harmonic generation

Pamela Jordan, Jon Cooper, Graeme McNay, Frances T. Docherty, Duncan Graham, W. Ewen Smith, Gavin Sinclair, and Miles J. Padgett

Open Access Open Access

Abstract

Silica particles were partially coated with silver, and a suitable chromophore, such that they could be trapped within an optical tweezers system and simultaneously be excited to emit a surface–enhanced resonance Raman scattering (SERRS) spectrum. A standard 1064 nm TEM00 mode laser was used to trap the bead whilst a frequency doubling crystal inserted into the beam gave several microwatts of 532 nm co-linear light to excite the SERRS emission. The configuration has clear applications in providing apparatus that can manipulate a particle whilst simultaneously obtaining surface sensitive sensory information.

©2005 Optical Society of America

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Figures (4)
Fig. 1.
Fig. 1. Schematic diagram of experimental set-up.

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Fig. 2.
Fig. 2. Photo-degradation of several different individually trapped, silver and dye-coated particles using 436 mW of 1064 nm laser light which, after transmission through the frequency doubling crystal, introduced 10.9 µW of 532 nm excitation light in the trapping plane.

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Fig. 3.
Fig. 3. Comparison of the spectra recorded from the silver and dye coated micro-particle in the optical tweezers to the same dye adsorbed onto a silver colloid and recorded using a dedicated Raman system.

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Fig. 4.
Fig. 4. The spectra of a SERRS active bead being trapped, released and re-trapped. The particle is only active when trapped; when it is not trapped the intensity falls down to noise levels.

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