Abstract

A model-based image reconstruction method, bioluminescence tomography (BLT), is described. BLT has the potential to spatially resolve bioluminescence associated with gene expression in vivo, thus, offering a tomographic molecular imaging method. The three-dimensional spatial map of reporter genes is recovered using a diffusion equation model-based, finite element reconstruction algorithm. The imaging method is demonstrated using both numerical simulations and phantom experiments.

© 2004 Optical Society of America

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References

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Annu. Rev. Biomed. Eng. (1)

C. Contag, M. Bachmann, �??Advances in in vivo bioluminescence imaging of gene express,�?? Annu. Rev. Biomed. Eng. 4, 235-260 (2002).
[CrossRef] [PubMed]

Appl. Opt. (1)

J. Biomed. Opt. (1)

B.W. Rice, M. Cable, M. Nelson, �??In vivo imaging of light-emitting probes,�?? J. Biomed. Opt. 6, 432-440 (2001).
[CrossRef] [PubMed]

J. Cellular Biochem. Suppl. (1)

S. Mandl, C. Schimmelpfennig, M. Edinger, R. Negrin, C. Contag, �??Understanding immune cell trafficking patterns via in vivo bioluminescence imaging,�?? J. Cellular Biochem. Suppl. 39, 239-248 (2002).
[CrossRef]

J. Opt. Soc. Am. A (2)

Med. Phys. (1)

K. Paulsen, H. Jiang, �??Spatially-varying optical property reconstruction using a finite element diffusion equation approximation,�?? Med. Phys. 22, 691-702 (1995).
[CrossRef] [PubMed]

Phys. Today (1)

A. Yodh, B. Chance, �??Spectroscopy and imgaing with diffusing light,�?? Phys. Today 48, 34-40 (1995).
[CrossRef]

Proc. Natl. Acad. Sci. USA (1)

S. Bhaumik, S. Gambhir, �??Optical imaging of Renilla luciferase reporter gene express in living mice,�?? Proc. Natl. Acad. Sci. USA 99, 377-382 (2002).
[CrossRef]

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Figures (2)

Fig 1.
Fig 1.

Source distribution (S(x,y,z)) images reconstructed from 748 simulated measurement data: (a) Exact image of the single target. (b) Reconstructed image of the single target. (c) A cross sectional image of the single target at X=10mm. (d) Exact image of the two targets. (e) Reconstructed image of the two targets. (f) A cross sectional image at Z=10mm of the two targets. The axes illustrate the spatial scale, in

Fig. 2.
Fig. 2.

Source distribution (S(x,y,z)) image of a single target reconstructed from 480 experimental data measured from the luciferase-luciferin containing phantom. (a) Exact image. (b) Reconstructed image. (c) A cross sectional image at X=10mm. The axes illustrate the spatial scale, in millimeters, whereas the color bar records the source coefficient (arbitrary units).

Equations (5)

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· D Φ ( x , y , z ) μ a Φ ( x , y , z ) = S ( x , y , z )
D Φ . i ̂ n = α Φ
[ A ] { Φ } = { b }
[ A ] { Φ S } = { b S }
( T + λ I ) Δ S = T ( Φ ( m ) Φ ( c ) )

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