Abstract

A multipurpose fiber fluorescence–reflection spectrometer with multiwave excitation is intended for biomedical studies, including studies in autofluorescence light. For this use, it is equipped with an LED-based illuminating system possessing a number of technical features: fluorescence excitation by radiation with wavelengths 365, 405, and 450 nm, synchronized switching of blocking filters at the excitation wavelength, and rapid switching of illumination with white light and the exciting radiation. This device makes it possible to observe changes of living objects from diffuse reflection and fluorescence spectra recorded virtually simultaneously. The possibilities of the device are illustrated by using it to study how the autofluorescence-excitation conditions affect the metabolic status of myocardium.

© 2014 Optical Society of America

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  1. V. B.  Loschenov, V. I.  Konov, A. M.  Prokhorov, “Photodynamic therapy and fluorescence diagnostics,” Laser Phys. 10, 1188 (2000).
  2. N. N.  Zharkova, D. N.  Kozlov, Yu. N.  Polivanov, R. L.  Pykhov, V. V.  Smirnov, “Laser-excited fluorescence spectrometric system for tissue diagnostics,” Proc. SPIE 2328, 196 (1994).
    [CrossRef]
  3. K. A.  Horvath, K. T.  Schomacker, C. C.  Lee, L. H.  Cohn, “Intraoperative myocardial ischemia detection with laser-induced fluorescence,” J. Thorac. Cardiovasc. Surg. 107, 220 (1994).
  4. H. A.  Zeng, N.  MacKinnon, R. W.  Cline, C. E.  MacAulay, “In vivo fluorescence spectroscopy of the gastrointestinal tract under multiple wavelength excitation,” Proc. SPIE 2926, 4 (1996).
    [CrossRef]
  5. A.  Zuluaga, U.  Utzinger, A.  Durkin, H.  Fuchs, A.  Gillenwater, R.  Jacob, B.  Kemp, J.  Fan, R.  Richards-Kortum, “Fluorescence excitation-emission matrices of human tissue: a system for in vivo measurement and data analysis,” Appl. Spectrosc. 53, 302 (1999).
    [CrossRef]
  6. K.  Uk, G. V.  Papayan, B. B.  Berezin, N. N.  Petrishchev, M. M.  Galagudza, “Spectrometer for fluorescence–reflection biomedical research,” Opt. Zh. 80, No. 1, 56 (2013) [J. Opt. Technol. 80, 40 (2013)].
  7. B.  Chance, B.  Schoener, R.  Oshino, F.  Itshak, “Oxidation–reduction ratio studies of NADH and flavoprotein fluorescence mitochondria in freeze-trapped samples signals,” J. Biol. Chem. 254, 4764 (1979).
  8. S. M.  Minasyan, M. M.  Galagudza, D. L.  Sonin, D. A.  Zverev, D. V.  Korolyëv, Yu. V.  Dmitriev, M. S.  Vasil’eva, Yu. N.  Grigorova, T. D.  Vlasov, “Technique for perfusing an isolated rat heart,” Region. Krov. Shchenie Mikrotsirk. 8, No. 4, 54 (2009).

2013 (1)

K.  Uk, G. V.  Papayan, B. B.  Berezin, N. N.  Petrishchev, M. M.  Galagudza, “Spectrometer for fluorescence–reflection biomedical research,” Opt. Zh. 80, No. 1, 56 (2013) [J. Opt. Technol. 80, 40 (2013)].

2009 (1)

S. M.  Minasyan, M. M.  Galagudza, D. L.  Sonin, D. A.  Zverev, D. V.  Korolyëv, Yu. V.  Dmitriev, M. S.  Vasil’eva, Yu. N.  Grigorova, T. D.  Vlasov, “Technique for perfusing an isolated rat heart,” Region. Krov. Shchenie Mikrotsirk. 8, No. 4, 54 (2009).

2000 (1)

V. B.  Loschenov, V. I.  Konov, A. M.  Prokhorov, “Photodynamic therapy and fluorescence diagnostics,” Laser Phys. 10, 1188 (2000).

1999 (1)

1996 (1)

H. A.  Zeng, N.  MacKinnon, R. W.  Cline, C. E.  MacAulay, “In vivo fluorescence spectroscopy of the gastrointestinal tract under multiple wavelength excitation,” Proc. SPIE 2926, 4 (1996).
[CrossRef]

1994 (2)

N. N.  Zharkova, D. N.  Kozlov, Yu. N.  Polivanov, R. L.  Pykhov, V. V.  Smirnov, “Laser-excited fluorescence spectrometric system for tissue diagnostics,” Proc. SPIE 2328, 196 (1994).
[CrossRef]

K. A.  Horvath, K. T.  Schomacker, C. C.  Lee, L. H.  Cohn, “Intraoperative myocardial ischemia detection with laser-induced fluorescence,” J. Thorac. Cardiovasc. Surg. 107, 220 (1994).

1979 (1)

B.  Chance, B.  Schoener, R.  Oshino, F.  Itshak, “Oxidation–reduction ratio studies of NADH and flavoprotein fluorescence mitochondria in freeze-trapped samples signals,” J. Biol. Chem. 254, 4764 (1979).

Berezin, B. B.

K.  Uk, G. V.  Papayan, B. B.  Berezin, N. N.  Petrishchev, M. M.  Galagudza, “Spectrometer for fluorescence–reflection biomedical research,” Opt. Zh. 80, No. 1, 56 (2013) [J. Opt. Technol. 80, 40 (2013)].

Chance, B.

B.  Chance, B.  Schoener, R.  Oshino, F.  Itshak, “Oxidation–reduction ratio studies of NADH and flavoprotein fluorescence mitochondria in freeze-trapped samples signals,” J. Biol. Chem. 254, 4764 (1979).

Cline, R. W.

H. A.  Zeng, N.  MacKinnon, R. W.  Cline, C. E.  MacAulay, “In vivo fluorescence spectroscopy of the gastrointestinal tract under multiple wavelength excitation,” Proc. SPIE 2926, 4 (1996).
[CrossRef]

Cohn, L. H.

K. A.  Horvath, K. T.  Schomacker, C. C.  Lee, L. H.  Cohn, “Intraoperative myocardial ischemia detection with laser-induced fluorescence,” J. Thorac. Cardiovasc. Surg. 107, 220 (1994).

Dmitriev, Yu. V.

S. M.  Minasyan, M. M.  Galagudza, D. L.  Sonin, D. A.  Zverev, D. V.  Korolyëv, Yu. V.  Dmitriev, M. S.  Vasil’eva, Yu. N.  Grigorova, T. D.  Vlasov, “Technique for perfusing an isolated rat heart,” Region. Krov. Shchenie Mikrotsirk. 8, No. 4, 54 (2009).

Durkin, A.

Fan, J.

Fuchs, H.

Galagudza, M. M.

K.  Uk, G. V.  Papayan, B. B.  Berezin, N. N.  Petrishchev, M. M.  Galagudza, “Spectrometer for fluorescence–reflection biomedical research,” Opt. Zh. 80, No. 1, 56 (2013) [J. Opt. Technol. 80, 40 (2013)].

S. M.  Minasyan, M. M.  Galagudza, D. L.  Sonin, D. A.  Zverev, D. V.  Korolyëv, Yu. V.  Dmitriev, M. S.  Vasil’eva, Yu. N.  Grigorova, T. D.  Vlasov, “Technique for perfusing an isolated rat heart,” Region. Krov. Shchenie Mikrotsirk. 8, No. 4, 54 (2009).

Gillenwater, A.

Grigorova, Yu. N.

S. M.  Minasyan, M. M.  Galagudza, D. L.  Sonin, D. A.  Zverev, D. V.  Korolyëv, Yu. V.  Dmitriev, M. S.  Vasil’eva, Yu. N.  Grigorova, T. D.  Vlasov, “Technique for perfusing an isolated rat heart,” Region. Krov. Shchenie Mikrotsirk. 8, No. 4, 54 (2009).

Horvath, K. A.

K. A.  Horvath, K. T.  Schomacker, C. C.  Lee, L. H.  Cohn, “Intraoperative myocardial ischemia detection with laser-induced fluorescence,” J. Thorac. Cardiovasc. Surg. 107, 220 (1994).

Itshak, F.

B.  Chance, B.  Schoener, R.  Oshino, F.  Itshak, “Oxidation–reduction ratio studies of NADH and flavoprotein fluorescence mitochondria in freeze-trapped samples signals,” J. Biol. Chem. 254, 4764 (1979).

Jacob, R.

Kemp, B.

Konov, V. I.

V. B.  Loschenov, V. I.  Konov, A. M.  Prokhorov, “Photodynamic therapy and fluorescence diagnostics,” Laser Phys. 10, 1188 (2000).

Korolyëv, D. V.

S. M.  Minasyan, M. M.  Galagudza, D. L.  Sonin, D. A.  Zverev, D. V.  Korolyëv, Yu. V.  Dmitriev, M. S.  Vasil’eva, Yu. N.  Grigorova, T. D.  Vlasov, “Technique for perfusing an isolated rat heart,” Region. Krov. Shchenie Mikrotsirk. 8, No. 4, 54 (2009).

Kozlov, D. N.

N. N.  Zharkova, D. N.  Kozlov, Yu. N.  Polivanov, R. L.  Pykhov, V. V.  Smirnov, “Laser-excited fluorescence spectrometric system for tissue diagnostics,” Proc. SPIE 2328, 196 (1994).
[CrossRef]

Lee, C. C.

K. A.  Horvath, K. T.  Schomacker, C. C.  Lee, L. H.  Cohn, “Intraoperative myocardial ischemia detection with laser-induced fluorescence,” J. Thorac. Cardiovasc. Surg. 107, 220 (1994).

Loschenov, V. B.

V. B.  Loschenov, V. I.  Konov, A. M.  Prokhorov, “Photodynamic therapy and fluorescence diagnostics,” Laser Phys. 10, 1188 (2000).

MacAulay, C. E.

H. A.  Zeng, N.  MacKinnon, R. W.  Cline, C. E.  MacAulay, “In vivo fluorescence spectroscopy of the gastrointestinal tract under multiple wavelength excitation,” Proc. SPIE 2926, 4 (1996).
[CrossRef]

MacKinnon, N.

H. A.  Zeng, N.  MacKinnon, R. W.  Cline, C. E.  MacAulay, “In vivo fluorescence spectroscopy of the gastrointestinal tract under multiple wavelength excitation,” Proc. SPIE 2926, 4 (1996).
[CrossRef]

Minasyan, S. M.

S. M.  Minasyan, M. M.  Galagudza, D. L.  Sonin, D. A.  Zverev, D. V.  Korolyëv, Yu. V.  Dmitriev, M. S.  Vasil’eva, Yu. N.  Grigorova, T. D.  Vlasov, “Technique for perfusing an isolated rat heart,” Region. Krov. Shchenie Mikrotsirk. 8, No. 4, 54 (2009).

Oshino, R.

B.  Chance, B.  Schoener, R.  Oshino, F.  Itshak, “Oxidation–reduction ratio studies of NADH and flavoprotein fluorescence mitochondria in freeze-trapped samples signals,” J. Biol. Chem. 254, 4764 (1979).

Papayan, G. V.

K.  Uk, G. V.  Papayan, B. B.  Berezin, N. N.  Petrishchev, M. M.  Galagudza, “Spectrometer for fluorescence–reflection biomedical research,” Opt. Zh. 80, No. 1, 56 (2013) [J. Opt. Technol. 80, 40 (2013)].

Petrishchev, N. N.

K.  Uk, G. V.  Papayan, B. B.  Berezin, N. N.  Petrishchev, M. M.  Galagudza, “Spectrometer for fluorescence–reflection biomedical research,” Opt. Zh. 80, No. 1, 56 (2013) [J. Opt. Technol. 80, 40 (2013)].

Polivanov, Yu. N.

N. N.  Zharkova, D. N.  Kozlov, Yu. N.  Polivanov, R. L.  Pykhov, V. V.  Smirnov, “Laser-excited fluorescence spectrometric system for tissue diagnostics,” Proc. SPIE 2328, 196 (1994).
[CrossRef]

Prokhorov, A. M.

V. B.  Loschenov, V. I.  Konov, A. M.  Prokhorov, “Photodynamic therapy and fluorescence diagnostics,” Laser Phys. 10, 1188 (2000).

Pykhov, R. L.

N. N.  Zharkova, D. N.  Kozlov, Yu. N.  Polivanov, R. L.  Pykhov, V. V.  Smirnov, “Laser-excited fluorescence spectrometric system for tissue diagnostics,” Proc. SPIE 2328, 196 (1994).
[CrossRef]

Richards-Kortum, R.

Schoener, B.

B.  Chance, B.  Schoener, R.  Oshino, F.  Itshak, “Oxidation–reduction ratio studies of NADH and flavoprotein fluorescence mitochondria in freeze-trapped samples signals,” J. Biol. Chem. 254, 4764 (1979).

Schomacker, K. T.

K. A.  Horvath, K. T.  Schomacker, C. C.  Lee, L. H.  Cohn, “Intraoperative myocardial ischemia detection with laser-induced fluorescence,” J. Thorac. Cardiovasc. Surg. 107, 220 (1994).

Smirnov, V. V.

N. N.  Zharkova, D. N.  Kozlov, Yu. N.  Polivanov, R. L.  Pykhov, V. V.  Smirnov, “Laser-excited fluorescence spectrometric system for tissue diagnostics,” Proc. SPIE 2328, 196 (1994).
[CrossRef]

Sonin, D. L.

S. M.  Minasyan, M. M.  Galagudza, D. L.  Sonin, D. A.  Zverev, D. V.  Korolyëv, Yu. V.  Dmitriev, M. S.  Vasil’eva, Yu. N.  Grigorova, T. D.  Vlasov, “Technique for perfusing an isolated rat heart,” Region. Krov. Shchenie Mikrotsirk. 8, No. 4, 54 (2009).

Uk, K.

K.  Uk, G. V.  Papayan, B. B.  Berezin, N. N.  Petrishchev, M. M.  Galagudza, “Spectrometer for fluorescence–reflection biomedical research,” Opt. Zh. 80, No. 1, 56 (2013) [J. Opt. Technol. 80, 40 (2013)].

Utzinger, U.

Vasil’eva, M. S.

S. M.  Minasyan, M. M.  Galagudza, D. L.  Sonin, D. A.  Zverev, D. V.  Korolyëv, Yu. V.  Dmitriev, M. S.  Vasil’eva, Yu. N.  Grigorova, T. D.  Vlasov, “Technique for perfusing an isolated rat heart,” Region. Krov. Shchenie Mikrotsirk. 8, No. 4, 54 (2009).

Vlasov, T. D.

S. M.  Minasyan, M. M.  Galagudza, D. L.  Sonin, D. A.  Zverev, D. V.  Korolyëv, Yu. V.  Dmitriev, M. S.  Vasil’eva, Yu. N.  Grigorova, T. D.  Vlasov, “Technique for perfusing an isolated rat heart,” Region. Krov. Shchenie Mikrotsirk. 8, No. 4, 54 (2009).

Zeng, H. A.

H. A.  Zeng, N.  MacKinnon, R. W.  Cline, C. E.  MacAulay, “In vivo fluorescence spectroscopy of the gastrointestinal tract under multiple wavelength excitation,” Proc. SPIE 2926, 4 (1996).
[CrossRef]

Zharkova, N. N.

N. N.  Zharkova, D. N.  Kozlov, Yu. N.  Polivanov, R. L.  Pykhov, V. V.  Smirnov, “Laser-excited fluorescence spectrometric system for tissue diagnostics,” Proc. SPIE 2328, 196 (1994).
[CrossRef]

Zuluaga, A.

Zverev, D. A.

S. M.  Minasyan, M. M.  Galagudza, D. L.  Sonin, D. A.  Zverev, D. V.  Korolyëv, Yu. V.  Dmitriev, M. S.  Vasil’eva, Yu. N.  Grigorova, T. D.  Vlasov, “Technique for perfusing an isolated rat heart,” Region. Krov. Shchenie Mikrotsirk. 8, No. 4, 54 (2009).

Appl. Spectrosc. (1)

J. Biol. Chem. (1)

B.  Chance, B.  Schoener, R.  Oshino, F.  Itshak, “Oxidation–reduction ratio studies of NADH and flavoprotein fluorescence mitochondria in freeze-trapped samples signals,” J. Biol. Chem. 254, 4764 (1979).

J. Thorac. Cardiovasc. Surg. (1)

K. A.  Horvath, K. T.  Schomacker, C. C.  Lee, L. H.  Cohn, “Intraoperative myocardial ischemia detection with laser-induced fluorescence,” J. Thorac. Cardiovasc. Surg. 107, 220 (1994).

Laser Phys. (1)

V. B.  Loschenov, V. I.  Konov, A. M.  Prokhorov, “Photodynamic therapy and fluorescence diagnostics,” Laser Phys. 10, 1188 (2000).

Opt. Zh. (1)

K.  Uk, G. V.  Papayan, B. B.  Berezin, N. N.  Petrishchev, M. M.  Galagudza, “Spectrometer for fluorescence–reflection biomedical research,” Opt. Zh. 80, No. 1, 56 (2013) [J. Opt. Technol. 80, 40 (2013)].

Proc. SPIE (2)

N. N.  Zharkova, D. N.  Kozlov, Yu. N.  Polivanov, R. L.  Pykhov, V. V.  Smirnov, “Laser-excited fluorescence spectrometric system for tissue diagnostics,” Proc. SPIE 2328, 196 (1994).
[CrossRef]

H. A.  Zeng, N.  MacKinnon, R. W.  Cline, C. E.  MacAulay, “In vivo fluorescence spectroscopy of the gastrointestinal tract under multiple wavelength excitation,” Proc. SPIE 2926, 4 (1996).
[CrossRef]

Region. Krov. Shchenie Mikrotsirk. (1)

S. M.  Minasyan, M. M.  Galagudza, D. L.  Sonin, D. A.  Zverev, D. V.  Korolyëv, Yu. V.  Dmitriev, M. S.  Vasil’eva, Yu. N.  Grigorova, T. D.  Vlasov, “Technique for perfusing an isolated rat heart,” Region. Krov. Shchenie Mikrotsirk. 8, No. 4, 54 (2009).

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