Abstract

This paper presents a theoretical and experimental study of the possibilities of using a holographic method of polarization contrast to solve the problem of three-dimensional visualization of anisotropic microobjects. The experimental studies were carried out using a digital holographic interference microscope. It is shown that the polarization-contrast method makes it possible to obtain three-dimensional images of anisotropic microobjects with the maximum contrast and high quality, and this increases the sensitivity and resolution of the method by comparison with other optical methods. The possibilities of combining holographic methods of polarization contrast and interference contrast for the three-dimensional visualization of anisotropic microobjects have been studied for the first time.

© 2012 OSA

PDF Article

References

  • View by:
  • |
  • |
  • |

  1. M. Françon, “Phase-contrast microscopy,” in Progress in Microscopy (Row, Peterson and Company, New York, 1961; Gosizdat Fiz.-Mat. Lit., Moscow, 1961), pp. 64–93.
  2. T. V. Tishko, V. P. Titar’, and D. N. Tishko, “Holographic methods of three-dimensional visualization of microscopic phase objects,” Opt. Zh. 72, No. 2, 48 (2005). [J. Opt. Technol. 72, 203 (2005)].
  3. V. L. Novitskiĭ, N. V. Ryazantseva, A. E. Stepovaya, N. M. Shevtsova, A. A. Miller, B. N. Zaĭtsev, T. V. Tishko, V. P. Titar’, and D. N. Tishko, Theory and Practice of Erythrocyte Microscopy (Pechatnaya Manufaktura, Tomsk, 2008).
  4. D. N. Tishko, T. V. Tishko, and V. P. Titar’, “Using digital holographic microscopy to study transparent thin films,” Opt. Zh. 76, No. 3, 40 (2009). [J. Opt. Technol. 76, 147 (2009)].
  5. T. V. Tishko, V. P. Titar, D. N. Tishko, and K. V. Nosov, “Digital holographic interference microscopy in the study of 3D morphology and functionality of human blood erythrocytes,” Laser Phys. 18, No. 4, 1 (2008).
    [CrossRef]
  6. T. V. Tishko, D. N. Tishko, and V. P. Titar, “Holographic method for blood cells imaging,” Imaging Microsc. No. 3, 46 (2009).
    [CrossRef]
  7. D. N. Tishko, T. V. Tishko, and V. P. Titar, “Application of the digital holographic interference microscopy for thin transparent films investigation,” Prakt. Metallogr. 47, 719 (2010).
  8. T. V. Tishko, D. N. Tishko, and V. P. Titar, “3D morphology of phase microscopic objects by the digital holographic interference microscopy method,” in Geomorphology and Plate Tectonics, eds., D. M. Ferrari and A. R. Guiseppi(Nova Publishers, New York, 2009), pp. 345–357.
  9. T. V. Tishko, D. N. Tishko, and V. P. Titar, “3D imaging of phase microscopic objects by digital holographic method,” in 3D Imaging: Theory, Technology and Application, eds., E. H. Duke and S. R. Aquirre(Nova Publishers, New York, 2010), pp. 51–92.
  10. T. V. Tishko, D. N. Tishko, and V. P. Titar, “Holographic methods for phase micro-objects study,” in Microscopy: Science, Technology, Application and Education, eds., A Mendez-Vilas and J. D. Alvarez(Formatex Research Center, 2010), vol. 3, pp. 2117–2128.
  11. G. S. Safronov, T. V. Tishko, and A. M. Garagataya, “Polarization filtering in holographic microscopy,” Ukr. Fiz. Zh. 28, 1472 (1983).
  12. G. S. Safronov, T. V. Tishko, and A. M. Garagataya, “Holographic polarization microscope,” Prib. Tekh. Éksp. No. 1, 237 (1984).

2010 (1)

D. N. Tishko, T. V. Tishko, and V. P. Titar, “Application of the digital holographic interference microscopy for thin transparent films investigation,” Prakt. Metallogr. 47, 719 (2010).

2009 (2)

D. N. Tishko, T. V. Tishko, and V. P. Titar’, “Using digital holographic microscopy to study transparent thin films,” Opt. Zh. 76, No. 3, 40 (2009). [J. Opt. Technol. 76, 147 (2009)].

T. V. Tishko, D. N. Tishko, and V. P. Titar, “Holographic method for blood cells imaging,” Imaging Microsc. No. 3, 46 (2009).
[CrossRef]

2008 (1)

T. V. Tishko, V. P. Titar, D. N. Tishko, and K. V. Nosov, “Digital holographic interference microscopy in the study of 3D morphology and functionality of human blood erythrocytes,” Laser Phys. 18, No. 4, 1 (2008).
[CrossRef]

2005 (1)

T. V. Tishko, V. P. Titar’, and D. N. Tishko, “Holographic methods of three-dimensional visualization of microscopic phase objects,” Opt. Zh. 72, No. 2, 48 (2005). [J. Opt. Technol. 72, 203 (2005)].

1984 (1)

G. S. Safronov, T. V. Tishko, and A. M. Garagataya, “Holographic polarization microscope,” Prib. Tekh. Éksp. No. 1, 237 (1984).

1983 (1)

G. S. Safronov, T. V. Tishko, and A. M. Garagataya, “Polarization filtering in holographic microscopy,” Ukr. Fiz. Zh. 28, 1472 (1983).

Françon, M.

M. Françon, “Phase-contrast microscopy,” in Progress in Microscopy (Row, Peterson and Company, New York, 1961; Gosizdat Fiz.-Mat. Lit., Moscow, 1961), pp. 64–93.

Garagataya, A. M.

G. S. Safronov, T. V. Tishko, and A. M. Garagataya, “Holographic polarization microscope,” Prib. Tekh. Éksp. No. 1, 237 (1984).

G. S. Safronov, T. V. Tishko, and A. M. Garagataya, “Polarization filtering in holographic microscopy,” Ukr. Fiz. Zh. 28, 1472 (1983).

Miller, A. A.

V. L. Novitskiĭ, N. V. Ryazantseva, A. E. Stepovaya, N. M. Shevtsova, A. A. Miller, B. N. Zaĭtsev, T. V. Tishko, V. P. Titar’, and D. N. Tishko, Theory and Practice of Erythrocyte Microscopy (Pechatnaya Manufaktura, Tomsk, 2008).

Nosov, K. V.

T. V. Tishko, V. P. Titar, D. N. Tishko, and K. V. Nosov, “Digital holographic interference microscopy in the study of 3D morphology and functionality of human blood erythrocytes,” Laser Phys. 18, No. 4, 1 (2008).
[CrossRef]

Novitskii, V. L.

V. L. Novitskiĭ, N. V. Ryazantseva, A. E. Stepovaya, N. M. Shevtsova, A. A. Miller, B. N. Zaĭtsev, T. V. Tishko, V. P. Titar’, and D. N. Tishko, Theory and Practice of Erythrocyte Microscopy (Pechatnaya Manufaktura, Tomsk, 2008).

Ryazantseva, N. V.

V. L. Novitskiĭ, N. V. Ryazantseva, A. E. Stepovaya, N. M. Shevtsova, A. A. Miller, B. N. Zaĭtsev, T. V. Tishko, V. P. Titar’, and D. N. Tishko, Theory and Practice of Erythrocyte Microscopy (Pechatnaya Manufaktura, Tomsk, 2008).

Safronov, G. S.

G. S. Safronov, T. V. Tishko, and A. M. Garagataya, “Holographic polarization microscope,” Prib. Tekh. Éksp. No. 1, 237 (1984).

G. S. Safronov, T. V. Tishko, and A. M. Garagataya, “Polarization filtering in holographic microscopy,” Ukr. Fiz. Zh. 28, 1472 (1983).

Shevtsova, N. M.

V. L. Novitskiĭ, N. V. Ryazantseva, A. E. Stepovaya, N. M. Shevtsova, A. A. Miller, B. N. Zaĭtsev, T. V. Tishko, V. P. Titar’, and D. N. Tishko, Theory and Practice of Erythrocyte Microscopy (Pechatnaya Manufaktura, Tomsk, 2008).

Stepovaya, A. E.

V. L. Novitskiĭ, N. V. Ryazantseva, A. E. Stepovaya, N. M. Shevtsova, A. A. Miller, B. N. Zaĭtsev, T. V. Tishko, V. P. Titar’, and D. N. Tishko, Theory and Practice of Erythrocyte Microscopy (Pechatnaya Manufaktura, Tomsk, 2008).

Tishko, D. N.

D. N. Tishko, T. V. Tishko, and V. P. Titar, “Application of the digital holographic interference microscopy for thin transparent films investigation,” Prakt. Metallogr. 47, 719 (2010).

T. V. Tishko, D. N. Tishko, and V. P. Titar, “Holographic method for blood cells imaging,” Imaging Microsc. No. 3, 46 (2009).
[CrossRef]

D. N. Tishko, T. V. Tishko, and V. P. Titar’, “Using digital holographic microscopy to study transparent thin films,” Opt. Zh. 76, No. 3, 40 (2009). [J. Opt. Technol. 76, 147 (2009)].

T. V. Tishko, V. P. Titar, D. N. Tishko, and K. V. Nosov, “Digital holographic interference microscopy in the study of 3D morphology and functionality of human blood erythrocytes,” Laser Phys. 18, No. 4, 1 (2008).
[CrossRef]

T. V. Tishko, V. P. Titar’, and D. N. Tishko, “Holographic methods of three-dimensional visualization of microscopic phase objects,” Opt. Zh. 72, No. 2, 48 (2005). [J. Opt. Technol. 72, 203 (2005)].

V. L. Novitskiĭ, N. V. Ryazantseva, A. E. Stepovaya, N. M. Shevtsova, A. A. Miller, B. N. Zaĭtsev, T. V. Tishko, V. P. Titar’, and D. N. Tishko, Theory and Practice of Erythrocyte Microscopy (Pechatnaya Manufaktura, Tomsk, 2008).

T. V. Tishko, D. N. Tishko, and V. P. Titar, “3D morphology of phase microscopic objects by the digital holographic interference microscopy method,” in Geomorphology and Plate Tectonics, eds., D. M. Ferrari and A. R. Guiseppi(Nova Publishers, New York, 2009), pp. 345–357.

T. V. Tishko, D. N. Tishko, and V. P. Titar, “Holographic methods for phase micro-objects study,” in Microscopy: Science, Technology, Application and Education, eds., A Mendez-Vilas and J. D. Alvarez(Formatex Research Center, 2010), vol. 3, pp. 2117–2128.

T. V. Tishko, D. N. Tishko, and V. P. Titar, “3D imaging of phase microscopic objects by digital holographic method,” in 3D Imaging: Theory, Technology and Application, eds., E. H. Duke and S. R. Aquirre(Nova Publishers, New York, 2010), pp. 51–92.

Tishko, T. V.

D. N. Tishko, T. V. Tishko, and V. P. Titar, “Application of the digital holographic interference microscopy for thin transparent films investigation,” Prakt. Metallogr. 47, 719 (2010).

T. V. Tishko, D. N. Tishko, and V. P. Titar, “Holographic method for blood cells imaging,” Imaging Microsc. No. 3, 46 (2009).
[CrossRef]

D. N. Tishko, T. V. Tishko, and V. P. Titar’, “Using digital holographic microscopy to study transparent thin films,” Opt. Zh. 76, No. 3, 40 (2009). [J. Opt. Technol. 76, 147 (2009)].

T. V. Tishko, V. P. Titar, D. N. Tishko, and K. V. Nosov, “Digital holographic interference microscopy in the study of 3D morphology and functionality of human blood erythrocytes,” Laser Phys. 18, No. 4, 1 (2008).
[CrossRef]

T. V. Tishko, V. P. Titar’, and D. N. Tishko, “Holographic methods of three-dimensional visualization of microscopic phase objects,” Opt. Zh. 72, No. 2, 48 (2005). [J. Opt. Technol. 72, 203 (2005)].

G. S. Safronov, T. V. Tishko, and A. M. Garagataya, “Holographic polarization microscope,” Prib. Tekh. Éksp. No. 1, 237 (1984).

G. S. Safronov, T. V. Tishko, and A. M. Garagataya, “Polarization filtering in holographic microscopy,” Ukr. Fiz. Zh. 28, 1472 (1983).

T. V. Tishko, D. N. Tishko, and V. P. Titar, “3D morphology of phase microscopic objects by the digital holographic interference microscopy method,” in Geomorphology and Plate Tectonics, eds., D. M. Ferrari and A. R. Guiseppi(Nova Publishers, New York, 2009), pp. 345–357.

T. V. Tishko, D. N. Tishko, and V. P. Titar, “3D imaging of phase microscopic objects by digital holographic method,” in 3D Imaging: Theory, Technology and Application, eds., E. H. Duke and S. R. Aquirre(Nova Publishers, New York, 2010), pp. 51–92.

V. L. Novitskiĭ, N. V. Ryazantseva, A. E. Stepovaya, N. M. Shevtsova, A. A. Miller, B. N. Zaĭtsev, T. V. Tishko, V. P. Titar’, and D. N. Tishko, Theory and Practice of Erythrocyte Microscopy (Pechatnaya Manufaktura, Tomsk, 2008).

T. V. Tishko, D. N. Tishko, and V. P. Titar, “Holographic methods for phase micro-objects study,” in Microscopy: Science, Technology, Application and Education, eds., A Mendez-Vilas and J. D. Alvarez(Formatex Research Center, 2010), vol. 3, pp. 2117–2128.

Titar, V. P.

D. N. Tishko, T. V. Tishko, and V. P. Titar, “Application of the digital holographic interference microscopy for thin transparent films investigation,” Prakt. Metallogr. 47, 719 (2010).

T. V. Tishko, D. N. Tishko, and V. P. Titar, “Holographic method for blood cells imaging,” Imaging Microsc. No. 3, 46 (2009).
[CrossRef]

T. V. Tishko, V. P. Titar, D. N. Tishko, and K. V. Nosov, “Digital holographic interference microscopy in the study of 3D morphology and functionality of human blood erythrocytes,” Laser Phys. 18, No. 4, 1 (2008).
[CrossRef]

T. V. Tishko, D. N. Tishko, and V. P. Titar, “3D morphology of phase microscopic objects by the digital holographic interference microscopy method,” in Geomorphology and Plate Tectonics, eds., D. M. Ferrari and A. R. Guiseppi(Nova Publishers, New York, 2009), pp. 345–357.

T. V. Tishko, D. N. Tishko, and V. P. Titar, “3D imaging of phase microscopic objects by digital holographic method,” in 3D Imaging: Theory, Technology and Application, eds., E. H. Duke and S. R. Aquirre(Nova Publishers, New York, 2010), pp. 51–92.

T. V. Tishko, D. N. Tishko, and V. P. Titar, “Holographic methods for phase micro-objects study,” in Microscopy: Science, Technology, Application and Education, eds., A Mendez-Vilas and J. D. Alvarez(Formatex Research Center, 2010), vol. 3, pp. 2117–2128.

Titar’, V. P.

D. N. Tishko, T. V. Tishko, and V. P. Titar’, “Using digital holographic microscopy to study transparent thin films,” Opt. Zh. 76, No. 3, 40 (2009). [J. Opt. Technol. 76, 147 (2009)].

T. V. Tishko, V. P. Titar’, and D. N. Tishko, “Holographic methods of three-dimensional visualization of microscopic phase objects,” Opt. Zh. 72, No. 2, 48 (2005). [J. Opt. Technol. 72, 203 (2005)].

V. L. Novitskiĭ, N. V. Ryazantseva, A. E. Stepovaya, N. M. Shevtsova, A. A. Miller, B. N. Zaĭtsev, T. V. Tishko, V. P. Titar’, and D. N. Tishko, Theory and Practice of Erythrocyte Microscopy (Pechatnaya Manufaktura, Tomsk, 2008).

Zaitsev, B. N.

V. L. Novitskiĭ, N. V. Ryazantseva, A. E. Stepovaya, N. M. Shevtsova, A. A. Miller, B. N. Zaĭtsev, T. V. Tishko, V. P. Titar’, and D. N. Tishko, Theory and Practice of Erythrocyte Microscopy (Pechatnaya Manufaktura, Tomsk, 2008).

Imaging Microsc. (1)

T. V. Tishko, D. N. Tishko, and V. P. Titar, “Holographic method for blood cells imaging,” Imaging Microsc. No. 3, 46 (2009).
[CrossRef]

Laser Phys. (1)

T. V. Tishko, V. P. Titar, D. N. Tishko, and K. V. Nosov, “Digital holographic interference microscopy in the study of 3D morphology and functionality of human blood erythrocytes,” Laser Phys. 18, No. 4, 1 (2008).
[CrossRef]

Opt. Zh. (2)

T. V. Tishko, V. P. Titar’, and D. N. Tishko, “Holographic methods of three-dimensional visualization of microscopic phase objects,” Opt. Zh. 72, No. 2, 48 (2005). [J. Opt. Technol. 72, 203 (2005)].

D. N. Tishko, T. V. Tishko, and V. P. Titar’, “Using digital holographic microscopy to study transparent thin films,” Opt. Zh. 76, No. 3, 40 (2009). [J. Opt. Technol. 76, 147 (2009)].

Prakt. Metallogr. (1)

D. N. Tishko, T. V. Tishko, and V. P. Titar, “Application of the digital holographic interference microscopy for thin transparent films investigation,” Prakt. Metallogr. 47, 719 (2010).

Prib. Tekh. Éksp. (1)

G. S. Safronov, T. V. Tishko, and A. M. Garagataya, “Holographic polarization microscope,” Prib. Tekh. Éksp. No. 1, 237 (1984).

Ukr. Fiz. Zh. (1)

G. S. Safronov, T. V. Tishko, and A. M. Garagataya, “Polarization filtering in holographic microscopy,” Ukr. Fiz. Zh. 28, 1472 (1983).

Other (5)

T. V. Tishko, D. N. Tishko, and V. P. Titar, “3D morphology of phase microscopic objects by the digital holographic interference microscopy method,” in Geomorphology and Plate Tectonics, eds., D. M. Ferrari and A. R. Guiseppi(Nova Publishers, New York, 2009), pp. 345–357.

T. V. Tishko, D. N. Tishko, and V. P. Titar, “3D imaging of phase microscopic objects by digital holographic method,” in 3D Imaging: Theory, Technology and Application, eds., E. H. Duke and S. R. Aquirre(Nova Publishers, New York, 2010), pp. 51–92.

T. V. Tishko, D. N. Tishko, and V. P. Titar, “Holographic methods for phase micro-objects study,” in Microscopy: Science, Technology, Application and Education, eds., A Mendez-Vilas and J. D. Alvarez(Formatex Research Center, 2010), vol. 3, pp. 2117–2128.

M. Françon, “Phase-contrast microscopy,” in Progress in Microscopy (Row, Peterson and Company, New York, 1961; Gosizdat Fiz.-Mat. Lit., Moscow, 1961), pp. 64–93.

V. L. Novitskiĭ, N. V. Ryazantseva, A. E. Stepovaya, N. M. Shevtsova, A. A. Miller, B. N. Zaĭtsev, T. V. Tishko, V. P. Titar’, and D. N. Tishko, Theory and Practice of Erythrocyte Microscopy (Pechatnaya Manufaktura, Tomsk, 2008).

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.