Abstract

This paper discusses a method of constructing a depth map of a scene from a set of microscope images of the scene. For each coordinate of the image field, the number of the layer in which the variance over a window of definite size takes its maximum value is determined. It is proposed to preprocess the images before the stage of computing the variance, and the operation of separating the image of the object from the background is to be carried out by means of an adaptive threshold with respect to the difference of the maximum and minimum variance over all the layers for each pixel separately. Experimental studies show that the proposed method is suitable for constructing depth maps of various microscopic objects.

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  1. A. P. Pentland, “Depth of scene from depth of field,” in Proc. Image-Understanding Workshop, 1982, pp. 253–259.
  2. M. Niederoest, J. Niederoest, and J. Skucka, “Automatic 3D reconstruction and visualization of microscopic objects from a monoscopic multifocus image sequence,” in ISPRS Archives, XXXIV-5/W10 (2003).
  3. G. L. Greenberg and A. Boyde, “Direct-view 3-D microscopy using conventional lenses,” Eur. Microsc. Analysis 5, (1997).
  4. D. Schmitz, D. Seibt, R. Herpers, and W. Heiden, “3D-reconstruction of microscopic translucent silicate-based marine and freshwater organisms,” in Aktuelle Methoden der Laser- und Medizinphysik: Tagungsband der 2. Remagener Physiktage (2004), pp. 43–48.
  5. G. Packroff, J. R. Lawrence, and T. R. Neu, “In-situ confocal laser scanning microscopy of protozoans in cultures and complex biofilm communities,” Acta Protozool. 41, 245 (2002).
  6. V. Gaganov and A. Ignatenko, “Robust shape from focus via Markov random fields,” in Proc. Graphicon Conf., 2009, pp. 74–80.

2002 (1)

G. Packroff, J. R. Lawrence, and T. R. Neu, “In-situ confocal laser scanning microscopy of protozoans in cultures and complex biofilm communities,” Acta Protozool. 41, 245 (2002).

1997 (1)

G. L. Greenberg and A. Boyde, “Direct-view 3-D microscopy using conventional lenses,” Eur. Microsc. Analysis 5, (1997).

Boyde, A.

G. L. Greenberg and A. Boyde, “Direct-view 3-D microscopy using conventional lenses,” Eur. Microsc. Analysis 5, (1997).

Gaganov, V.

V. Gaganov and A. Ignatenko, “Robust shape from focus via Markov random fields,” in Proc. Graphicon Conf., 2009, pp. 74–80.

Greenberg, G. L.

G. L. Greenberg and A. Boyde, “Direct-view 3-D microscopy using conventional lenses,” Eur. Microsc. Analysis 5, (1997).

Heiden, W.

D. Schmitz, D. Seibt, R. Herpers, and W. Heiden, “3D-reconstruction of microscopic translucent silicate-based marine and freshwater organisms,” in Aktuelle Methoden der Laser- und Medizinphysik: Tagungsband der 2. Remagener Physiktage (2004), pp. 43–48.

Herpers, R.

D. Schmitz, D. Seibt, R. Herpers, and W. Heiden, “3D-reconstruction of microscopic translucent silicate-based marine and freshwater organisms,” in Aktuelle Methoden der Laser- und Medizinphysik: Tagungsband der 2. Remagener Physiktage (2004), pp. 43–48.

Ignatenko, A.

V. Gaganov and A. Ignatenko, “Robust shape from focus via Markov random fields,” in Proc. Graphicon Conf., 2009, pp. 74–80.

Lawrence, J. R.

G. Packroff, J. R. Lawrence, and T. R. Neu, “In-situ confocal laser scanning microscopy of protozoans in cultures and complex biofilm communities,” Acta Protozool. 41, 245 (2002).

Neu, T. R.

G. Packroff, J. R. Lawrence, and T. R. Neu, “In-situ confocal laser scanning microscopy of protozoans in cultures and complex biofilm communities,” Acta Protozool. 41, 245 (2002).

Niederoest, J.

M. Niederoest, J. Niederoest, and J. Skucka, “Automatic 3D reconstruction and visualization of microscopic objects from a monoscopic multifocus image sequence,” in ISPRS Archives, XXXIV-5/W10 (2003).

Niederoest, M.

M. Niederoest, J. Niederoest, and J. Skucka, “Automatic 3D reconstruction and visualization of microscopic objects from a monoscopic multifocus image sequence,” in ISPRS Archives, XXXIV-5/W10 (2003).

Packroff, G.

G. Packroff, J. R. Lawrence, and T. R. Neu, “In-situ confocal laser scanning microscopy of protozoans in cultures and complex biofilm communities,” Acta Protozool. 41, 245 (2002).

Pentland, A. P.

A. P. Pentland, “Depth of scene from depth of field,” in Proc. Image-Understanding Workshop, 1982, pp. 253–259.

Schmitz, D.

D. Schmitz, D. Seibt, R. Herpers, and W. Heiden, “3D-reconstruction of microscopic translucent silicate-based marine and freshwater organisms,” in Aktuelle Methoden der Laser- und Medizinphysik: Tagungsband der 2. Remagener Physiktage (2004), pp. 43–48.

Seibt, D.

D. Schmitz, D. Seibt, R. Herpers, and W. Heiden, “3D-reconstruction of microscopic translucent silicate-based marine and freshwater organisms,” in Aktuelle Methoden der Laser- und Medizinphysik: Tagungsband der 2. Remagener Physiktage (2004), pp. 43–48.

Skucka, J.

M. Niederoest, J. Niederoest, and J. Skucka, “Automatic 3D reconstruction and visualization of microscopic objects from a monoscopic multifocus image sequence,” in ISPRS Archives, XXXIV-5/W10 (2003).

Acta Protozool. (1)

G. Packroff, J. R. Lawrence, and T. R. Neu, “In-situ confocal laser scanning microscopy of protozoans in cultures and complex biofilm communities,” Acta Protozool. 41, 245 (2002).

Eur. Microsc. Analysis (1)

G. L. Greenberg and A. Boyde, “Direct-view 3-D microscopy using conventional lenses,” Eur. Microsc. Analysis 5, (1997).

Other (4)

D. Schmitz, D. Seibt, R. Herpers, and W. Heiden, “3D-reconstruction of microscopic translucent silicate-based marine and freshwater organisms,” in Aktuelle Methoden der Laser- und Medizinphysik: Tagungsband der 2. Remagener Physiktage (2004), pp. 43–48.

A. P. Pentland, “Depth of scene from depth of field,” in Proc. Image-Understanding Workshop, 1982, pp. 253–259.

M. Niederoest, J. Niederoest, and J. Skucka, “Automatic 3D reconstruction and visualization of microscopic objects from a monoscopic multifocus image sequence,” in ISPRS Archives, XXXIV-5/W10 (2003).

V. Gaganov and A. Ignatenko, “Robust shape from focus via Markov random fields,” in Proc. Graphicon Conf., 2009, pp. 74–80.

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