Abstract

The effectiveness of the surgical removal of glial tumors of the brain largely depends on the visualization of the malignant growth loci. The amino acid 5-aminolevulinic acid, which induces the accumulation of protoporphyrin IX in tumor cells, is used for these purposes. It is detected by the red fluorescence that characterizes porphyrins. Various methods are used for fluorescence visualization. This paper compares them, using test solutions with various concentrations of coproporphyrin III. The results showed that methods in which the exciting radiation is completely blocked are more sensitive by a factor of 4.3–4.7 by comparison with the traditional method, in which part of the exciting radiation is used to visualize the entire operating field in reflected blue light. Either coherent or incoherent light sources can be used to implement the new approach, with the former being preferable. The advantages are illustrated with examples of clinical studies.

© 2017 Optical Society of America

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