A multipurpose fiber fluorescence–reflection spectrometer with multiwave excitation is intended for biomedical studies, including studies in autofluorescence light. For this use, it is equipped with an LED-based illuminating system possessing a number of technical features: fluorescence excitation by radiation with wavelengths 365, 405, and 450 nm, synchronized switching of blocking filters at the excitation wavelength, and rapid switching of illumination with white light and the exciting radiation. This device makes it possible to observe changes of living objects from diffuse reflection and fluorescence spectra recorded virtually simultaneously. The possibilities of the device are illustrated by using it to study how the autofluorescence-excitation conditions affect the metabolic status of myocardium.
© 2014 Optical Society of AmericaPDF Article