Abstract

In light microscopy, spatial resolution is limited by diffraction effect. Confocal microscopy has improved resolutions in both lateral and axial directions, but these are still limited by diffraction effect. Confocal self-interference microscopy (CSIM) uses interference between two perpendicularly polarized beams to enhance lateral resolution. In previous research, we proposed a calcite plate with its optic-axis perpendicular to the propagation angle and one of the boundary surfaces of the plate. This type of plate is not widely used to our knowledge. In this paper, we change the calcite plate to more common one, which is commercially available. This calcite plate has its optic axis in the plane of incidence. We analyze the characteristics of this calcite plate and numerically compare the performances of CSIM in previous research and CSIM with the commercial calcite plate. Numerical results show improved performance when using the commercial calcite plate

© 2005 Optical Society of Korea

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  1. J. B. Pawley, Handbook of biological confocal microscopy (Plenum Press, New York, 1995)
  2. M. Vaez-lravani and D. 1. Kavaldjiev, "Resolution beyond the diffraction limit using frequency-domain field confinement in scanning microscopy," Ultramicroscopy vol. 61, pp. 105-110, 1995
    [CrossRef]
  3. C. J. R. Sheppard, "Binary optics and confocal imaging," Opt. Lett. vol. 24, no. 8, pp. 505-507, 1999
    [CrossRef]
  4. M. A. A. Neil, R. Juskaitis, T. Wilson and Z. J. Laczik, "Optimized pupil-plane filters for confocal microscope point- spread function engineering," Opt. Lett. vol. 25, no. 4, pp. 245-247, 2000
    [CrossRef]
  5. L. Liu, X. Deng, L. Yang, G. Wang, and Z. Xu, "Effect of an annular pupil ftIter on differencial confocal microscopy," Opt. Lett. vol. 25, no. 23, pp. 1711-1713, 2000
    [CrossRef]
  6. S. W. Hell, M. Schrader, and H. T. M. Van Der Voort, "Far- field fluorescence microscopy with three-dimentional resolution in the 100-nm range," 1. Microsc.-Oxford, vol. 187, no. 1, pp. 1-7, 1997
    [CrossRef]
  7. D. Kang and D. Gweon, "Enhancement of lateral resolution in confocal self-interference microscopy," Opt. Lett. vol. 28, no. 24, pp. 2470-2472, 2003
    [CrossRef]
  8. M. Born and E. Wolf, Principles of Optics (Cambridge University Press, UK, 1999)

2003

D. Kang and D. Gweon, "Enhancement of lateral resolution in confocal self-interference microscopy," Opt. Lett. vol. 28, no. 24, pp. 2470-2472, 2003
[CrossRef]

2000

M. A. A. Neil, R. Juskaitis, T. Wilson and Z. J. Laczik, "Optimized pupil-plane filters for confocal microscope point- spread function engineering," Opt. Lett. vol. 25, no. 4, pp. 245-247, 2000
[CrossRef]

L. Liu, X. Deng, L. Yang, G. Wang, and Z. Xu, "Effect of an annular pupil ftIter on differencial confocal microscopy," Opt. Lett. vol. 25, no. 23, pp. 1711-1713, 2000
[CrossRef]

1999

M. Born and E. Wolf, Principles of Optics (Cambridge University Press, UK, 1999)

C. J. R. Sheppard, "Binary optics and confocal imaging," Opt. Lett. vol. 24, no. 8, pp. 505-507, 1999
[CrossRef]

1997

S. W. Hell, M. Schrader, and H. T. M. Van Der Voort, "Far- field fluorescence microscopy with three-dimentional resolution in the 100-nm range," 1. Microsc.-Oxford, vol. 187, no. 1, pp. 1-7, 1997
[CrossRef]

1995

J. B. Pawley, Handbook of biological confocal microscopy (Plenum Press, New York, 1995)

M. Vaez-lravani and D. 1. Kavaldjiev, "Resolution beyond the diffraction limit using frequency-domain field confinement in scanning microscopy," Ultramicroscopy vol. 61, pp. 105-110, 1995
[CrossRef]

Journal of Microscopy

S. W. Hell, M. Schrader, and H. T. M. Van Der Voort, "Far- field fluorescence microscopy with three-dimentional resolution in the 100-nm range," 1. Microsc.-Oxford, vol. 187, no. 1, pp. 1-7, 1997
[CrossRef]

Optics Letters

D. Kang and D. Gweon, "Enhancement of lateral resolution in confocal self-interference microscopy," Opt. Lett. vol. 28, no. 24, pp. 2470-2472, 2003
[CrossRef]

C. J. R. Sheppard, "Binary optics and confocal imaging," Opt. Lett. vol. 24, no. 8, pp. 505-507, 1999
[CrossRef]

M. A. A. Neil, R. Juskaitis, T. Wilson and Z. J. Laczik, "Optimized pupil-plane filters for confocal microscope point- spread function engineering," Opt. Lett. vol. 25, no. 4, pp. 245-247, 2000
[CrossRef]

L. Liu, X. Deng, L. Yang, G. Wang, and Z. Xu, "Effect of an annular pupil ftIter on differencial confocal microscopy," Opt. Lett. vol. 25, no. 23, pp. 1711-1713, 2000
[CrossRef]

Ultramicroscopy

M. Vaez-lravani and D. 1. Kavaldjiev, "Resolution beyond the diffraction limit using frequency-domain field confinement in scanning microscopy," Ultramicroscopy vol. 61, pp. 105-110, 1995
[CrossRef]

Other

J. B. Pawley, Handbook of biological confocal microscopy (Plenum Press, New York, 1995)

M. Born and E. Wolf, Principles of Optics (Cambridge University Press, UK, 1999)

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