We have developed a fluorescence optical detection system using a digital
micromirror device (DMD) for monitoring 3D cell culture matrices in situ. Full 3D
imaging with fast scanning speed was implemented by the combined action of a DMD and
a motorized stage. Imaging results with fluorescent microbeads measure the minimum
axial resolution of the system as 6.3 μm, while full
1-mm scanning through 3D alginate-based matrix was demonstrated. For cell imaging,
improved images were obtained by removing background fluorescence although the
scanning distance was reduced because of low intracellular fluorescence efficiency.
The system is expected to be useful to study various dynamics and behaviors of
3-dimensionally cultured cells in microfluidic systems.
© 2012 Optical Society of Korea
OSA participates in Crossref's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.