Abstract

Optical microscopes are widely used for medical imaging these days, but biopsy is a lengthy process that causes many problems during the ex-vivo imaging procedure. The endo-microscope has been studied to increase accessibility to the human body and to get in-vivo images to use for medical diagnosis. This research proposes a multi-modal confocal endo-microscope for bio-medical imaging. We introduce the design process for a small endoscopic probe and a coupling mechanism for the probe to make the multi-modal confocal endo-microscope. The endoscopic probe was designed to decrease chromatic and spherical aberrations, which deteriorate the images obtained with the conventional GRIN lens. Fluorescence and reflectance images of various samples were obtained with the proposed endo-microscope. We evaluated the performance of the proposed endo-microscope by analyzing the acquired images, and demonstrate the possibilities of in-vivo medical imaging for early diagnosis.

© 2011 Optical Society of Korea

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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
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2010 (3)

W.-H. Kim, C.-I. Kim, S.-W. Lee, S.-H. Lim, C.-W. Park, H. Lee, and M.-K. Park, "Particle image velocimetry of the blood flow in a micro-channel using the confocal laser scanning microscope," J. Opt. Soc. Korea 14, 42-48 (2010).
[CrossRef]

C.-S. Rim, "The optical design of probe-type microscope objective for intravital laser scanning CARS microendoscopy," J. Opt. Soc. Korea 14, 431-437 (2010).
[CrossRef]

W. Piyawattanametha and T. D. Wang, "MEMS-based dual-axes confocal microendoscopy," IEEE J. Select. Topics Quantum Electron. 16, 804-814 (2010).
[CrossRef]

2009 (2)

A. A. Tanbakuchi, A. R. Rouse, J. A. Udovich, K. D. Hatch, and A. F. Gmitro, "Clinical confocal microlaparoscope for real-time in vivo optical biopsies," Journal of Biomedical Optics 14, 044030-044012 (2009).
[CrossRef]

J. Tan, M. Quinn, J. Pyman, P. Delaney, and W. McLaren, "Detection of cervical intraepithelial neoplasia in vivo using confocal endomicroscopy," BJOG: An International Journal of Obstetrics & Gynaecology 116, 1663-1670 (2009).
[CrossRef]

2008 (1)

P. Kim, M. Puoris"haag, D. Côté, C. P. Lin, and S. H. Yun, "In vivo confocal and multiphoton microendoscopy," Journal of Biomedical Optics 13, 010501 (2008).

2007 (2)

E. J. Seibel, R. S. Johnston, C. M. Brown, J. A. Dominitz, and M. B. Kimmey, "Novel ultrathin scanning fiber endoscope for cholangioscopy and pancreatoscopy," Gastrointestinal Endoscopy 65, Ab125 (2007).

L. Fu and M. Gu, "Fibre-optic nonlinear optical microscopy and endoscopy," Journal of Microscopy-Oxford 226, 195-206 (2007).
[CrossRef]

2006 (1)

2004 (1)

2003 (1)

D. M. McDonald and P. L. Choyke, "Imaging of angiogenesis: from microscope to clinic," Nature Medicine 9, 713-725 (2003).
[CrossRef]

2002 (1)

K. B. Sung, C. N. Liang, M. Descour, T. Collier, M. Follen, and R. Richards-Kortum, "Fiber-optic confocal reflectance microscope with miniature objective for in vivo imaging of human tissues," IEEE Transactions on Biomedical Engineering 49, 1168-1172 (2002).
[CrossRef]

1999 (1)

D. Ren and J. R. Allington-Smith, "Apochromatic lenses for near-infrared astronomical instruments," Opt. Eng. 38, 537-542 (1999).
[CrossRef]

1998 (1)

J. M. Sasian and M. R. Descour, "Power distribution and symmetry in lens systems," Opt. Eng. 37, 1001-1004 (1998).
[CrossRef]

Appl. Opt. (1)

BJOG: An International Journal of Obstetrics & Gynaecology (1)

J. Tan, M. Quinn, J. Pyman, P. Delaney, and W. McLaren, "Detection of cervical intraepithelial neoplasia in vivo using confocal endomicroscopy," BJOG: An International Journal of Obstetrics & Gynaecology 116, 1663-1670 (2009).
[CrossRef]

Gastrointestinal Endoscopy (1)

E. J. Seibel, R. S. Johnston, C. M. Brown, J. A. Dominitz, and M. B. Kimmey, "Novel ultrathin scanning fiber endoscope for cholangioscopy and pancreatoscopy," Gastrointestinal Endoscopy 65, Ab125 (2007).

IEEE J. Select. Topics Quantum Electron. (1)

W. Piyawattanametha and T. D. Wang, "MEMS-based dual-axes confocal microendoscopy," IEEE J. Select. Topics Quantum Electron. 16, 804-814 (2010).
[CrossRef]

IEEE Transactions on Biomedical Engineering (1)

K. B. Sung, C. N. Liang, M. Descour, T. Collier, M. Follen, and R. Richards-Kortum, "Fiber-optic confocal reflectance microscope with miniature objective for in vivo imaging of human tissues," IEEE Transactions on Biomedical Engineering 49, 1168-1172 (2002).
[CrossRef]

Journal of Biomedical Optics (2)

P. Kim, M. Puoris"haag, D. Côté, C. P. Lin, and S. H. Yun, "In vivo confocal and multiphoton microendoscopy," Journal of Biomedical Optics 13, 010501 (2008).

A. A. Tanbakuchi, A. R. Rouse, J. A. Udovich, K. D. Hatch, and A. F. Gmitro, "Clinical confocal microlaparoscope for real-time in vivo optical biopsies," Journal of Biomedical Optics 14, 044030-044012 (2009).
[CrossRef]

Journal of Microscopy-Oxford (1)

L. Fu and M. Gu, "Fibre-optic nonlinear optical microscopy and endoscopy," Journal of Microscopy-Oxford 226, 195-206 (2007).
[CrossRef]

Journal of the Optical Society of Korea (2)

C.-S. Rim, "The optical design of probe-type microscope objective for intravital laser scanning CARS microendoscopy," J. Opt. Soc. Korea 14, 431-437 (2010).
[CrossRef]

W.-H. Kim, C.-I. Kim, S.-W. Lee, S.-H. Lim, C.-W. Park, H. Lee, and M.-K. Park, "Particle image velocimetry of the blood flow in a micro-channel using the confocal laser scanning microscope," J. Opt. Soc. Korea 14, 42-48 (2010).
[CrossRef]

Nature Medicine (1)

D. M. McDonald and P. L. Choyke, "Imaging of angiogenesis: from microscope to clinic," Nature Medicine 9, 713-725 (2003).
[CrossRef]

Opt. Eng. (2)

J. M. Sasian and M. R. Descour, "Power distribution and symmetry in lens systems," Opt. Eng. 37, 1001-1004 (1998).
[CrossRef]

D. Ren and J. R. Allington-Smith, "Apochromatic lenses for near-infrared astronomical instruments," Opt. Eng. 38, 537-542 (1999).
[CrossRef]

Opt. Lett. (1)

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