Abstract

Ultrashort pulse laser drilling of polycarbonate track-etched membrane (pTEM) material was used to fabricate a mouse embryo cell trapping device. Holes with a diameter of <TEX>$2{\mu}m$</TEX> to <TEX>$5{\mu}m$</TEX> were fabricated on a <TEX>$10{\mu}m$</TEX> thick membrane using a femtosecond laser with a 150 fs pulse width and 775 nm wavelength and multiple-pulse irradiation. In cell trapping tests, the overall cell occupancy of the machined holes in the fabricated pTEM was found to be more than 80%. The results of a single pulse and multiple pulse irradiation were compared in terms of the surface quality. It was generally found that a single pulse with high energy was less desirable than irradiation with multiple pulses of lower energy.

© 2009 Optical Society of Korea

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  1. B. Stuart, M. Feit, A. Rubenchik, B. Shore, and M. Perry, "Laser-induced damage in dielectrics with nanosecond to subpicosecond pulses," Physical Review Letters 74, 2248-2252 (1995)
    [CrossRef]
  2. S. Nolte, C. Momma, H. Jacobs, A. Tunnermann, B. Chichkov, B. Wellegehausen, and H. Welling, "Ablation of metals by ultrashort laser pulses," J. Opt. Soc. Am. B 14, 2716-2722 (1997)
    [CrossRef]
  3. J. Acker, "Bio-preservation of cells and engineered tissues," in Tissue Engineering II, T. Scheper, K. Lee, and D. Kaplan, ed. (Springer, Berlin, Germany, 2005)
    [CrossRef]
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    [CrossRef]
  5. X. Liu, D. Du, and G. Mourou, "Laser ablation and micromachining with ultrashort laser pulses," IEEE J. Quantum Electron. 33, 1706-1716 (1997)
    [CrossRef]
  6. D. Du, X. Liu, G. Korn, J. Squier, and G. Mourou, "Laser induced breakdown by impact ionization in SiO2 with pulse widths from 7ns to 150fs," Appl. Phys. Lett. 64, 3071-3073 (1994)
    [CrossRef]
  7. V. Kohli, J. Acker, and A. Elezzabi, "Reversible permeabilization using high-intensity femtosecond laser pulses: applications to biopreservation," Biotechnol. Bioeng. 92, 889-899 (2005)
    [CrossRef]
  8. L. MacQueen, M. Bushmann, and M. Wertheimer, "Gene delivery by electroporation after dielectro-phoretic positioning of cells in a non-uniform electric field," Bioelectrochemistry 72, 141-148 (2008)
    [CrossRef]
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    [CrossRef]
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    [CrossRef]
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  13. http://www.2spi.com/catalog/spec˙prep/filter3.html
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  15. K. Salonitis, A. Stournaras, G. Tsoukantas, P. Stavropoulos, and G. Chryssolouris, "A theoretical and experimental investigation on limitations of pulsed laser drilling," J. Mat. Proc. Technology 183, 96-103 (2007)
    [CrossRef]
  16. H. Choi, J. Johnson, J. Nam, D. Farson, and J. Lannutti, "Structuring electrospun polycaprolactone nanofiber tissue scaffolds by femtosecond laser ablation," J. Laser Applications 19, 225-231 (2007)
    [CrossRef]

2008 (3)

L. MacQueen, M. Bushmann, and M. Wertheimer, "Gene delivery by electroporation after dielectro-phoretic positioning of cells in a non-uniform electric field," Bioelectrochemistry 72, 141-148 (2008)
[CrossRef]

J. Zhang, P. Blackmore, B. Hargrave, S. Xiao, S. Beebe, and K. Schoenbach, "Nanosecond pulse electric field: a novel non-ligand agonist for platelet activation," Archives of Biochemistry and Biophysics 471, 240-248(2008)
[CrossRef]

H. Wang and C. Lu, "Microfluidic electroporation for delivery of small molecules and genes into cells using a common DC power supply," Biotechnol. Bioeng. 100, 579-586 (2008)
[CrossRef]

2007 (3)

A. Garner, G. Chen, N. Chen, V. Sridhara, J. Kolb, R. Swanson, S. Beebe, R. Joshi, and K. Schoenbach, "Ultrashort electric pulse induced changes in cellular dielectric properties," Biochem. Biophy. Research Comm. 362, 139-144 (2007)
[CrossRef]

K. Salonitis, A. Stournaras, G. Tsoukantas, P. Stavropoulos, and G. Chryssolouris, "A theoretical and experimental investigation on limitations of pulsed laser drilling," J. Mat. Proc. Technology 183, 96-103 (2007)
[CrossRef]

H. Choi, J. Johnson, J. Nam, D. Farson, and J. Lannutti, "Structuring electrospun polycaprolactone nanofiber tissue scaffolds by femtosecond laser ablation," J. Laser Applications 19, 225-231 (2007)
[CrossRef]

2005 (2)

J. Acker, "Bio-preservation of cells and engineered tissues," in Tissue Engineering II, T. Scheper, K. Lee, and D. Kaplan, ed. (Springer, Berlin, Germany, 2005)
[CrossRef]

V. Kohli, J. Acker, and A. Elezzabi, "Reversible permeabilization using high-intensity femtosecond laser pulses: applications to biopreservation," Biotechnol. Bioeng. 92, 889-899 (2005)
[CrossRef]

1997 (1)

X. Liu, D. Du, and G. Mourou, "Laser ablation and micromachining with ultrashort laser pulses," IEEE J. Quantum Electron. 33, 1706-1716 (1997)
[CrossRef]

1995 (1)

B. Stuart, M. Feit, A. Rubenchik, B. Shore, and M. Perry, "Laser-induced damage in dielectrics with nanosecond to subpicosecond pulses," Physical Review Letters 74, 2248-2252 (1995)
[CrossRef]

1994 (1)

D. Du, X. Liu, G. Korn, J. Squier, and G. Mourou, "Laser induced breakdown by impact ionization in SiO2 with pulse widths from 7ns to 150fs," Appl. Phys. Lett. 64, 3071-3073 (1994)
[CrossRef]

1989 (1)

M. Sasnett, "Propagation of multimode laser beams - the M2 factor," in The Physics and Technology of Laser Resonators, D. Hall and P. Jackson, ed. (IOP Publishing, Bristol, UK, 1989), pp. 132-142

1977 (1)

S. Nolte, C. Momma, H. Jacobs, A. Tunnermann, B. Chichkov, B. Wellegehausen, and H. Welling, "Ablation of metals by ultrashort laser pulses," J. Opt. Soc. Am. B 14, 2716-2722 (1997)
[CrossRef]

Appl. Phys. Lett. (1)

D. Du, X. Liu, G. Korn, J. Squier, and G. Mourou, "Laser induced breakdown by impact ionization in SiO2 with pulse widths from 7ns to 150fs," Appl. Phys. Lett. 64, 3071-3073 (1994)
[CrossRef]

Archives of Biochemistry and Biophysics (1)

J. Zhang, P. Blackmore, B. Hargrave, S. Xiao, S. Beebe, and K. Schoenbach, "Nanosecond pulse electric field: a novel non-ligand agonist for platelet activation," Archives of Biochemistry and Biophysics 471, 240-248(2008)
[CrossRef]

Biochemical and Biophysical Research Communications (1)

A. Garner, G. Chen, N. Chen, V. Sridhara, J. Kolb, R. Swanson, S. Beebe, R. Joshi, and K. Schoenbach, "Ultrashort electric pulse induced changes in cellular dielectric properties," Biochem. Biophy. Research Comm. 362, 139-144 (2007)
[CrossRef]

Bioelectrochemistry (1)

L. MacQueen, M. Bushmann, and M. Wertheimer, "Gene delivery by electroporation after dielectro-phoretic positioning of cells in a non-uniform electric field," Bioelectrochemistry 72, 141-148 (2008)
[CrossRef]

Biotechnology and Bioengineering (2)

V. Kohli, J. Acker, and A. Elezzabi, "Reversible permeabilization using high-intensity femtosecond laser pulses: applications to biopreservation," Biotechnol. Bioeng. 92, 889-899 (2005)
[CrossRef]

H. Wang and C. Lu, "Microfluidic electroporation for delivery of small molecules and genes into cells using a common DC power supply," Biotechnol. Bioeng. 100, 579-586 (2008)
[CrossRef]

IEEE J. Quantum Electron. (1)

X. Liu, D. Du, and G. Mourou, "Laser ablation and micromachining with ultrashort laser pulses," IEEE J. Quantum Electron. 33, 1706-1716 (1997)
[CrossRef]

J. Laser Applications (1)

H. Choi, J. Johnson, J. Nam, D. Farson, and J. Lannutti, "Structuring electrospun polycaprolactone nanofiber tissue scaffolds by femtosecond laser ablation," J. Laser Applications 19, 225-231 (2007)
[CrossRef]

JOSA B (1)

S. Nolte, C. Momma, H. Jacobs, A. Tunnermann, B. Chichkov, B. Wellegehausen, and H. Welling, "Ablation of metals by ultrashort laser pulses," J. Opt. Soc. Am. B 14, 2716-2722 (1997)
[CrossRef]

Journal of Materials Processing Technology (1)

K. Salonitis, A. Stournaras, G. Tsoukantas, P. Stavropoulos, and G. Chryssolouris, "A theoretical and experimental investigation on limitations of pulsed laser drilling," J. Mat. Proc. Technology 183, 96-103 (2007)
[CrossRef]

Phys. Rev. Lett. (1)

B. Stuart, M. Feit, A. Rubenchik, B. Shore, and M. Perry, "Laser-induced damage in dielectrics with nanosecond to subpicosecond pulses," Physical Review Letters 74, 2248-2252 (1995)
[CrossRef]

Other (5)

J. Acker, "Bio-preservation of cells and engineered tissues," in Tissue Engineering II, T. Scheper, K. Lee, and D. Kaplan, ed. (Springer, Berlin, Germany, 2005)
[CrossRef]

Wikipedia, http://en.wikipedia.org/wiki/Electroporation

http://www.whatman.com/

http://www.2spi.com/catalog/spec˙prep/filter3.html

M. Sasnett, "Propagation of multimode laser beams - the M2 factor," in The Physics and Technology of Laser Resonators, D. Hall and P. Jackson, ed. (IOP Publishing, Bristol, UK, 1989), pp. 132-142

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