Abstract

We demonstrated single-molecule fluorescence resonance energy transfer (FRET) from single donor-acceptor dye pair attached to a DNA with a setup based on a confocal microscope. Singlestrand DNAs were immobilized on a glass surface with suitable inter-dye distance. Energy transfer efficiency between the donor and the acceptor dyes attached to the DNA was measured with different lengths of DNA. Photobleaching of single dye molecule was observed and used as a sign of single-molecule detection. We could achieve high enough signal-to-noise ratio to detect the fluorescence from a single-molecule, which allows real-time observation of the distance change between single dye pairs in nanometer scale.

© 2008 Optical Society of Korea

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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
  7. T. Ha, A. Y. Ting, J. Liang, W. B. Caldwell, A. A. Deniz, D. S. Chemla, P. G. Schultz, and S. Weiss, Proc. Natl. Acad. Sci. 96, 893, 1999
    [CrossRef]
  8. T. Ha, D.S. Chemla, Th. Enderle, and S. Weiss, Appl. Phys. Lett. 70, 782, 1997
    [CrossRef]
  9. P. L. Southwick, L. A. Ernst, E. W. Tauriello, S. R. Parker, R. B. Mujumdar, S. R. Mujumdar, H. A. Clever, and A. S. Waggoner, Cytometry 11, 418, 1990
    [CrossRef]
  10. M. C. Murphy, I. Rasnik, W. Cheng, T. M. Lohman, and T. Ha, Biophys. J. 86, 2530, 2004
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    [CrossRef]
  12. I. Rasnik, S. McKinney, and T. Ha, Nat. Meth. 34, 891, 2006
    [CrossRef]

2007

P. V. Cornish, and T. Ha, ACS Chem. Biol. 2, 53, 2007
[CrossRef]

T. Mori, R. D. Vale, and M. Tomishige, Nature 450, 750, 2007
[CrossRef]

2006

I. Rasnik, S. McKinney, and T. Ha, Nat. Meth. 34, 891, 2006
[CrossRef]

2005

X. Zhuang, Annu. Rev. Biophys. Biomole. Strut. 34, 399, 2005
[CrossRef]

2004

T. Ha, Biochemistry 43, 4055, 2004
[CrossRef]

M. C. Murphy, I. Rasnik, W. Cheng, T. M. Lohman, and T. Ha, Biophys. J. 86, 2530, 2004

1999

T. Ha, A. Y. Ting, J. Liang, W. B. Caldwell, A. A. Deniz, D. S. Chemla, P. G. Schultz, and S. Weiss, Proc. Natl. Acad. Sci. 96, 893, 1999
[CrossRef]

1998

G. W. Gordon, G. Berry, X. H. Liang, B. Levine, and B. Herman, Biophys. J. 74, 2702, 1998

1997

T. Ha, D.S. Chemla, Th. Enderle, and S. Weiss, Appl. Phys. Lett. 70, 782, 1997
[CrossRef]

1990

P. L. Southwick, L. A. Ernst, E. W. Tauriello, S. R. Parker, R. B. Mujumdar, S. R. Mujumdar, H. A. Clever, and A. S. Waggoner, Cytometry 11, 418, 1990
[CrossRef]

1953

R. E. Benesch, and R. Benesch, Science 118, 447, 1953
[CrossRef]

1948

T. Forster, Ann. Phys., 2, 55, 1948
[CrossRef]

ACS Chem. Biol.

P. V. Cornish, and T. Ha, ACS Chem. Biol. 2, 53, 2007
[CrossRef]

Annalen der Physik

T. Forster, Ann. Phys., 2, 55, 1948
[CrossRef]

Annual Review of Biophysics and Biomolecular Structure

X. Zhuang, Annu. Rev. Biophys. Biomole. Strut. 34, 399, 2005
[CrossRef]

Appl. Phys. Lett.

T. Ha, D.S. Chemla, Th. Enderle, and S. Weiss, Appl. Phys. Lett. 70, 782, 1997
[CrossRef]

Biochemistry

T. Ha, Biochemistry 43, 4055, 2004
[CrossRef]

Biophys. J.

G. W. Gordon, G. Berry, X. H. Liang, B. Levine, and B. Herman, Biophys. J. 74, 2702, 1998

M. C. Murphy, I. Rasnik, W. Cheng, T. M. Lohman, and T. Ha, Biophys. J. 86, 2530, 2004

Cytometry Part A

P. L. Southwick, L. A. Ernst, E. W. Tauriello, S. R. Parker, R. B. Mujumdar, S. R. Mujumdar, H. A. Clever, and A. S. Waggoner, Cytometry 11, 418, 1990
[CrossRef]

Nature

T. Mori, R. D. Vale, and M. Tomishige, Nature 450, 750, 2007
[CrossRef]

Nature Methods

I. Rasnik, S. McKinney, and T. Ha, Nat. Meth. 34, 891, 2006
[CrossRef]

PNAS

T. Ha, A. Y. Ting, J. Liang, W. B. Caldwell, A. A. Deniz, D. S. Chemla, P. G. Schultz, and S. Weiss, Proc. Natl. Acad. Sci. 96, 893, 1999
[CrossRef]

Science

R. E. Benesch, and R. Benesch, Science 118, 447, 1953
[CrossRef]

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