Abstract

Tomographic phase microscopy (TPM) is an emerging optical microscopic technique for bioimaging. TPM uses digital holographic measurements of complex scattered fields to reconstruct three-dimensional refractive index (RI) maps of cells with diffraction-limited resolution by solving inverse scattering problems. In this paper, we review the developments of TPM from the fundamental physics to its applications in bioimaging. We first provide a comprehensive description of the tomographic reconstruction physical models used in TPM. The RI map reconstruction algorithms and various regularization methods are discussed. Selected TPM applications for cellular imaging, particularly in hematology, are reviewed. Finally, we examine the limitations of current TPM systems, propose future solutions, and envision promising directions in biomedical research.

© 2017 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Comparative study of iterative reconstruction algorithms for missing cone problems in optical diffraction tomography

JooWon Lim, KyeoReh Lee, Kyong Hwan Jin, Seungwoo Shin, SeoEun Lee, YongKeun Park, and Jong Chul Ye
Opt. Express 23(13) 16933-16948 (2015)

Three-dimensional quantitative phase imaging via tomographic deconvolution phase microscopy

Micah H. Jenkins and Thomas K. Gaylord
Appl. Opt. 54(31) 9213-9227 (2015)

Label-free characterization of white blood cells by measuring 3D refractive index maps

Jonghee Yoon, Kyoohyun Kim, HyunJoo Park, Chulhee Choi, Seongsoo Jang, and YongKeun Park
Biomed. Opt. Express 6(10) 3865-3875 (2015)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (7)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (18)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription